BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137825.9 - phase: 0
(319 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87917 homologue to SP|Q03943|IM30_PEA Membrane-associated 30 k... 403 e-163
TC79532 weakly similar to GP|8164030|gb|AAF73960.1| SGS3 {Arabid... 35 0.038
TC86734 similar to PIR|D86257|D86257 hypothetical protein [impor... 35 0.038
TC84723 similar to GP|16945387|emb|CAD11800. conserved hypotheti... 31 0.72
TC77503 weakly similar to GP|16930417|gb|AAL31894.1 At2g24420/T2... 31 0.72
TC77611 SP|P48488|PP1_MEDVA Serine/threonine protein phosphatase... 31 0.72
TC90947 similar to GP|17381164|gb|AAL36394.1 unknown protein {Ar... 30 0.94
TC77855 similar to SP|O04336|WR21_ARATH Probable WRKY transcript... 29 2.1
TC77173 similar to GP|15293109|gb|AAK93665.1 putative yeast pher... 29 2.1
TC87542 weakly similar to GP|22136436|gb|AAM91296.1 putative pro... 29 2.7
TC79135 weakly similar to GP|22136510|gb|AAM91333.1 unknown prot... 29 2.7
BG453901 similar to GP|15983376|gb AT3g59080/F17J16_130 {Arabido... 28 3.6
BQ751178 28 3.6
TC90068 similar to GP|20197988|gb|AAD22321.2 expressed protein {... 28 3.6
TC90398 28 3.6
BF644858 weakly similar to GP|5679842|emb l1332.6 {Oryza sativa}... 28 3.6
TC92243 similar to PIR|T06661|T06661 hypothetical protein T6G15.... 28 4.7
TC89432 weakly similar to GP|9294592|dbj|BAB02873.1 protein kina... 27 8.0
TC78339 similar to PIR|T51937|T51937 probable inositol-1 4 5-tri... 27 8.0
TC91734 weakly similar to GP|16604661|gb|AAL24123.1 unknown prot... 27 8.0
>TC87917 homologue to SP|Q03943|IM30_PEA Membrane-associated 30 kDa protein
chloroplast precursor (M30). [Garden pea] {Pisum
sativum}, partial (92%)
Length = 1291
Score = 403 bits (1035), Expect(3) = e-163
Identities = 213/213 (100%), Positives = 213/213 (100%)
Frame = +1
Query: 1 MATKLQIFTQLPSAPLQSSSSSSSLRKPLATSFFGTRPANTVKFRGMRIAKPVRGGGAIG 60
MATKLQIFTQLPSAPLQSSSSSSSLRKPLATSFFGTRPANTVKFRGMRIAKPVRGGGAIG
Sbjct: 94 MATKLQIFTQLPSAPLQSSSSSSSLRKPLATSFFGTRPANTVKFRGMRIAKPVRGGGAIG 273
Query: 61 AGMNLFERFTRVVKSYANAIVSSFEDPEKILEQAVLEMNDDLTKMRQATAQVLASQKRME 120
AGMNLFERFTRVVKSYANAIVSSFEDPEKILEQAVLEMNDDLTKMRQATAQVLASQKRME
Sbjct: 274 AGMNLFERFTRVVKSYANAIVSSFEDPEKILEQAVLEMNDDLTKMRQATAQVLASQKRME 453
Query: 121 NKYKAATQASEEWYRKAQLALQKGEEDLAREALKRRKSFADNASALKAQLDQQKGVVDSL 180
NKYKAATQASEEWYRKAQLALQKGEEDLAREALKRRKSFADNASALKAQLDQQKGVVDSL
Sbjct: 454 NKYKAATQASEEWYRKAQLALQKGEEDLAREALKRRKSFADNASALKAQLDQQKGVVDSL 633
Query: 181 VSNTRLLESKIQEARSKKDTLKARAQSAKTSTK 213
VSNTRLLESKIQEARSKKDTLKARAQSAKTSTK
Sbjct: 634 VSNTRLLESKIQEARSKKDTLKARAQSAKTSTK 732
Score = 142 bits (359), Expect(3) = e-163
Identities = 72/72 (100%), Positives = 72/72 (100%)
Frame = +3
Query: 248 GQLTSDDLEGKFAMLESSSVDDDLANLKKELSGSSKKGELPPGRSSTRTGTPFRDADIET 307
GQLTSDDLEGKFAMLESSSVDDDLANLKKELSGSSKKGELPPGRSSTRTGTPFRDADIET
Sbjct: 837 GQLTSDDLEGKFAMLESSSVDDDLANLKKELSGSSKKGELPPGRSSTRTGTPFRDADIET 1016
Query: 308 ELEQLRQRSKEF 319
ELEQLRQRSKEF
Sbjct: 1017ELEQLRQRSKEF 1052
Score = 70.1 bits (170), Expect(3) = e-163
Identities = 35/38 (92%), Positives = 37/38 (97%)
Frame = +2
Query: 213 KVSEMLGNVNTSGALSAFEKMEEKVMTMESQAEALGQL 250
+VSEMLGNVNTSGALSAFEKMEEKVMTMESQAEALG +
Sbjct: 731 RVSEMLGNVNTSGALSAFEKMEEKVMTMESQAEALGSV 844
>TC79532 weakly similar to GP|8164030|gb|AAF73960.1| SGS3 {Arabidopsis
thaliana}, partial (47%)
Length = 1558
Score = 35.0 bits (79), Expect = 0.038
Identities = 21/61 (34%), Positives = 31/61 (50%), Gaps = 3/61 (4%)
Frame = -3
Query: 9 TQLPSAPLQSSSSSSSLRKPLATSFFGTRPANTVKFRG---MRIAKPVRGGGAIGAGMNL 65
+ + S QSSSSSSS + SFF + + T+ F + P RGGG +G + L
Sbjct: 722 SSVSSRSSQSSSSSSSSSSSSSPSFFCSNSSGTISFLA*VDFQFQPPCRGGGMMGLEVTL 543
Query: 66 F 66
+
Sbjct: 542 Y 540
>TC86734 similar to PIR|D86257|D86257 hypothetical protein [imported] -
Arabidopsis thaliana, partial (78%)
Length = 1429
Score = 35.0 bits (79), Expect = 0.038
Identities = 20/46 (43%), Positives = 28/46 (60%), Gaps = 2/46 (4%)
Frame = +2
Query: 1 MATKLQ--IFTQLPSAPLQSSSSSSSLRKPLATSFFGTRPANTVKF 44
MA LQ ++ + S+P SSSSSSS+R + SFF + P T+ F
Sbjct: 47 MAATLQTPLYAAISSSPASSSSSSSSIRTTTSFSFFRSTPPPTLLF 184
>TC84723 similar to GP|16945387|emb|CAD11800. conserved hypothetical protein
{Neurospora crassa}, partial (5%)
Length = 999
Score = 30.8 bits (68), Expect = 0.72
Identities = 25/103 (24%), Positives = 50/103 (48%), Gaps = 3/103 (2%)
Frame = +3
Query: 110 AQVLASQKRMENKYKAATQASEEWYRKAQLALQKGEED--LAREALKRRKSFADNASAL- 166
A++ +++ ++ + SEE + K+Q+ LQK +E+ LA +A +R K AD
Sbjct: 594 AELQRAKRDLDEIRNREARISEEKHLKSQVELQKYQEEMRLAEKAKQREKEAADAVERFQ 773
Query: 167 KAQLDQQKGVVDSLVSNTRLLESKIQEARSKKDTLKARAQSAK 209
K + ++ + + ++QE R K+ A+A+ K
Sbjct: 774 KKEQERLAKQEKEKQEREKEFQRRLQEERQKELDRIAKAKKEK 902
>TC77503 weakly similar to GP|16930417|gb|AAL31894.1 At2g24420/T28I24.15
{Arabidopsis thaliana}, partial (54%)
Length = 1787
Score = 30.8 bits (68), Expect = 0.72
Identities = 32/123 (26%), Positives = 56/123 (45%)
Frame = +2
Query: 162 NASALKAQLDQQKGVVDSLVSNTRLLESKIQEARSKKDTLKARAQSAKTSTKVSEMLGNV 221
++SALK QLDQ L S + LES+I E K LK + Q K+ +
Sbjct: 203 DSSALKIQLDQ-------LNSKIQSLESQISE---KTQELKKKDQVIAEKEKLFQ----- 337
Query: 222 NTSGALSAFEKMEEKVMTMESQAEALGQLTSDDLEGKFAMLESSSVDDDLANLKKELSGS 281
LS+ + ++ +V +++ + G L +++ GK A + + + LK EL
Sbjct: 338 ---DKLSSIQSLQNEVASLQKK----GSLDAEEQVGK-AYARAGELQKQVDKLKSELEAQ 493
Query: 282 SKK 284
+ +
Sbjct: 494 NSE 502
>TC77611 SP|P48488|PP1_MEDVA Serine/threonine protein phosphatase PP1 (EC
3.1.3.16). {Medicago varia}, complete
Length = 1509
Score = 30.8 bits (68), Expect = 0.72
Identities = 16/34 (47%), Positives = 21/34 (61%)
Frame = +2
Query: 266 SVDDDLANLKKELSGSSKKGELPPGRSSTRTGTP 299
SVDD L + L S KKG++ G +S+R GTP
Sbjct: 1025 SVDDSLTCSFQILKSSDKKGKVGFGNNSSRPGTP 1126
>TC90947 similar to GP|17381164|gb|AAL36394.1 unknown protein {Arabidopsis
thaliana}, partial (55%)
Length = 676
Score = 30.4 bits (67), Expect = 0.94
Identities = 29/107 (27%), Positives = 47/107 (43%), Gaps = 7/107 (6%)
Frame = +3
Query: 93 QAVLEMNDDLTKMRQATAQVLASQKRMENKYKAATQA-------SEEWYRKAQLALQKGE 145
+A L + D+ + + AQVL N KAA++A +E W + L GE
Sbjct: 249 EAALTLAPDVPALHEQKAQVLLEIGDAWNALKAASRATELKPSWAEAWVTLGRAQLNYGE 428
Query: 146 EDLAREALKRRKSFADNASALKAQLDQQKGVVDSLVSNTRLLESKIQ 192
D A E+ D A ALK ++ + D + +RL++ + Q
Sbjct: 429 PDNAIESF-------DRALALKPDYEEAQ---DDRKTASRLIKKRKQ 539
>TC77855 similar to SP|O04336|WR21_ARATH Probable WRKY transcription factor
21 (WRKY DNA-binding protein 21). [Mouse-ear cress],
partial (38%)
Length = 1507
Score = 29.3 bits (64), Expect = 2.1
Identities = 25/107 (23%), Positives = 45/107 (41%), Gaps = 5/107 (4%)
Frame = +3
Query: 192 QEARSKKDTLKARAQSAKTSTKVSEMLGNVNTSGALSAFEKMEEKVMTMESQAEALGQLT 251
Q+ ++ L + Q + +EM+ NTSG F+K TM S + L+
Sbjct: 612 QQQLQQQRLLMQQQQQQQQMKHQAEMMFRRNTSGISLNFDKATSSTPTMSSTRSFISSLS 791
Query: 252 SD----DLEG-KFAMLESSSVDDDLANLKKELSGSSKKGELPPGRSS 293
D +++G F ++ + D + K++ S +G L SS
Sbjct: 792 IDGSVANVDGSSFHLIGAPISSDQNSQHKRKCSARGDEGSLKCSSSS 932
>TC77173 similar to GP|15293109|gb|AAK93665.1 putative yeast pheromone
receptor protein AR781 {Arabidopsis thaliana}, partial
(20%)
Length = 1380
Score = 29.3 bits (64), Expect = 2.1
Identities = 27/113 (23%), Positives = 50/113 (43%)
Frame = +3
Query: 206 QSAKTSTKVSEMLGNVNTSGALSAFEKMEEKVMTMESQAEALGQLTSDDLEGKFAMLESS 265
+S K+ST+ +E L + G + F E KV+ + Q Q D+ G+ E
Sbjct: 393 ESNKSSTRSAEELFH---GGKIKPFTNEEAKVVVPKKQ-----QKVQDERRGR----ERE 536
Query: 266 SVDDDLANLKKELSGSSKKGELPPGRSSTRTGTPFRDADIETELEQLRQRSKE 318
++ L N GR S+R+ +P+R ++ +ELE+ + ++
Sbjct: 537 RIESSLNN---------------SGRRSSRSHSPYRKSNYISELEEQNSQKQQ 650
>TC87542 weakly similar to GP|22136436|gb|AAM91296.1 putative protein
{Arabidopsis thaliana}, partial (39%)
Length = 2234
Score = 28.9 bits (63), Expect = 2.7
Identities = 34/149 (22%), Positives = 58/149 (38%), Gaps = 1/149 (0%)
Frame = +2
Query: 136 KAQLALQKGEEDLAREALKRRKSFADNASALKAQLDQQKGVVDSLVSNTRLLESKIQEAR 195
K + L E L ++ K ADN S LKA + VV +E K + +
Sbjct: 1142 KRKSVLMDELEKLLALVKQKEKEIADNDSDLKAVEHKINNVVSGFEEIQSTIEVKYGKLQ 1321
Query: 196 SKKDTLKARAQS-AKTSTKVSEMLGNVNTSGALSAFEKMEEKVMTMESQAEALGQLTSDD 254
S +K ++ + K+ +L GA K+ E E +A+ ++
Sbjct: 1322 SVLAQVKLETETLSLKKDKIDNLLVQEERMGA-----KLREFARVSEEEAKGYREIVKLR 1486
Query: 255 LEGKFAMLESSSVDDDLANLKKELSGSSK 283
++L+S L N +++LSG K
Sbjct: 1487 RSLMSSILKSGEDKLRLTNNEEKLSGEVK 1573
>TC79135 weakly similar to GP|22136510|gb|AAM91333.1 unknown protein
{Arabidopsis thaliana}, partial (15%)
Length = 1821
Score = 28.9 bits (63), Expect = 2.7
Identities = 23/74 (31%), Positives = 34/74 (45%)
Frame = +2
Query: 231 EKMEEKVMTMESQAEALGQLTSDDLEGKFAMLESSSVDDDLANLKKELSGSSKKGELPPG 290
E M +K + + E + +L +LEG+ SV D NL L G + E+P G
Sbjct: 1202 EDMNDKSDQLVEEGENVNELA*SELEGE-------SVGDLELNLGL-LLGKQQGKEVPQG 1357
Query: 291 RSSTRTGTPFRDAD 304
S + TP D+D
Sbjct: 1358 DSGIKEATPESDSD 1399
>BG453901 similar to GP|15983376|gb AT3g59080/F17J16_130 {Arabidopsis
thaliana}, partial (21%)
Length = 642
Score = 28.5 bits (62), Expect = 3.6
Identities = 29/125 (23%), Positives = 59/125 (47%), Gaps = 3/125 (2%)
Frame = +2
Query: 121 NKYKAATQASEEWYRKAQLALQKGEEDLAREALKRRKSFADNASALKAQLDQQKGVVD-S 179
+K+ ++SEE K Q KG++ A + F ++K +++ +K V+D S
Sbjct: 71 DKFGTTMKSSEESDDKGQ----KGDDFSAENPQNQSVKFHLKHISMKNEIEPKKSVIDYS 238
Query: 180 LVSNTRL--LESKIQEARSKKDTLKARAQSAKTSTKVSEMLGNVNTSGALSAFEKMEEKV 237
+ TR+ L +++ E + ++T+ +S K T + + A ++ E + V
Sbjct: 239 IRDLTRIQTLHTRVIE-KKNQNTISRLQKSTKKQTNSKQSYKPAVSPVAAASPEYSSQLV 415
Query: 238 MTMES 242
T+ES
Sbjct: 416 ATLES 430
>BQ751178
Length = 630
Score = 28.5 bits (62), Expect = 3.6
Identities = 21/63 (33%), Positives = 31/63 (48%)
Frame = +1
Query: 8 FTQLPSAPLQSSSSSSSLRKPLATSFFGTRPANTVKFRGMRIAKPVRGGGAIGAGMNLFE 67
+ QL S+ Q SS+++ RK ++SF P ++ + RGGGAIG G
Sbjct: 115 YLQLSSSSTQDSSATTPSRKTPSSSF----PNSSNL*ASPQFQVASRGGGAIGQGPATHP 282
Query: 68 RFT 70
R T
Sbjct: 283 RAT 291
>TC90068 similar to GP|20197988|gb|AAD22321.2 expressed protein {Arabidopsis
thaliana}, partial (88%)
Length = 1352
Score = 28.5 bits (62), Expect = 3.6
Identities = 12/37 (32%), Positives = 22/37 (59%)
Frame = -3
Query: 12 PSAPLQSSSSSSSLRKPLATSFFGTRPANTVKFRGMR 48
P LQS+ +++ RKP + FG RP ++ F+ ++
Sbjct: 1116 PRIDLQSNGNTNKSRKP*TITQFGIRPTSSNHFKSLK 1006
>TC90398
Length = 1434
Score = 28.5 bits (62), Expect = 3.6
Identities = 33/143 (23%), Positives = 62/143 (43%), Gaps = 8/143 (5%)
Frame = +3
Query: 153 LKRRKSFADNASALKAQLDQQKGVVDSLVSNTRLLESKIQEARSKKDTLKAR----AQSA 208
L ++ A L +L + V+D L + +LLE IQ+ S+ + ++ R A S
Sbjct: 495 LSESENSKSKAHELGIKLIGSQKVIDDLTTKVKLLEDTIQDRISQPEIVQERSIYEAPSL 674
Query: 209 KTSTKVSEMLGNVNTSGALSAFEKMEEKVMTMESQAEALGQLTSDDLEGKF-AMLESSSV 267
++++E+ ALS + ++ L D+ G+ ++ S+
Sbjct: 675 PAGSEITEVEEGSLGKKALSPVPLAAHVRNMRKGSSDHLAL----DIGGESDQLINSADT 842
Query: 268 DDDLANLKKELSGSS---KKGEL 287
DDD + K L+ S K+G+L
Sbjct: 843 DDDKGHAFKSLNTSGFVPKQGKL 911
>BF644858 weakly similar to GP|5679842|emb l1332.6 {Oryza sativa}, partial
(49%)
Length = 692
Score = 28.5 bits (62), Expect = 3.6
Identities = 34/144 (23%), Positives = 61/144 (41%)
Frame = +2
Query: 100 DDLTKMRQATAQVLASQKRMENKYKAATQASEEWYRKAQLALQKGEEDLAREALKRRKSF 159
+D ++ + +V+ S K ENK + +RK LQK E ++ ++A
Sbjct: 131 EDTQEVEEVQEEVI-SDKTSENKEEETRDEILARHRKEISQLQKKEVEMKKQA------- 286
Query: 160 ADNASALKAQLDQQKGVVDSLVSNTRLLESKIQEARSKKDTLKARAQSAKTSTKVSEMLG 219
A KA+ +K V+ VS L +K++E ++K+ L A S+ + S M
Sbjct: 287 ---ARGSKAEQKAKKRQVEEEVSQ---LSAKLKEKQAKE--LSALGYSSGIGNEKSNMDN 442
Query: 220 NVNTSGALSAFEKMEEKVMTMESQ 243
V +S + E ++ Q
Sbjct: 443 LVKAIAGVSVSSQPENTKVSKAKQ 514
>TC92243 similar to PIR|T06661|T06661 hypothetical protein T6G15.140 -
Arabidopsis thaliana, partial (43%)
Length = 723
Score = 28.1 bits (61), Expect = 4.7
Identities = 16/43 (37%), Positives = 22/43 (50%)
Frame = -3
Query: 3 TKLQIFTQLPSAPLQSSSSSSSLRKPLATSFFGTRPANTVKFR 45
++ F+ S+ SSSSS L P TSF G A+ + FR
Sbjct: 148 SRCDAFSFTSSSASASSSSSGLLLSPFLTSFEGRSHASLIDFR 20
>TC89432 weakly similar to GP|9294592|dbj|BAB02873.1 protein kinase-like
protein {Arabidopsis thaliana}, partial (51%)
Length = 1272
Score = 27.3 bits (59), Expect = 8.0
Identities = 16/37 (43%), Positives = 19/37 (51%)
Frame = -1
Query: 5 LQIFTQLPSAPLQSSSSSSSLRKPLATSFFGTRPANT 41
LQ+ Q+PS PL S S SSL FF R + T
Sbjct: 255 LQLLQQIPSLPLSSLLSVSSLLSVAHHWFFPNRHSQT 145
>TC78339 similar to PIR|T51937|T51937 probable inositol-1 4 5-trisphosphate
5-phosphatase (EC 3.1.3.56) At5P2 [imported] -
Arabidopsis, partial (53%)
Length = 1762
Score = 27.3 bits (59), Expect = 8.0
Identities = 13/56 (23%), Positives = 28/56 (49%)
Frame = +1
Query: 222 NTSGALSAFEKMEEKVMTMESQAEALGQLTSDDLEGKFAMLESSSVDDDLANLKKE 277
++SG L + K ++ + + + + ++ SDD + F L + DD+L K +
Sbjct: 964 HSSGNLGSLLKEQQVIPKVVDSLDDVSEMLSDDGDDAFIELPENQDDDELGTTKSQ 1131
>TC91734 weakly similar to GP|16604661|gb|AAL24123.1 unknown protein
{Arabidopsis thaliana}, partial (61%)
Length = 981
Score = 27.3 bits (59), Expect = 8.0
Identities = 43/192 (22%), Positives = 77/192 (39%), Gaps = 19/192 (9%)
Frame = +2
Query: 109 TAQVLASQKRMENKYKAATQASEEWYRKAQLALQKGEEDLAREALKRRKSFADNAS-ALK 167
T QV+ +EN +A E+W+++ + + G + + L+ KS N S AL+
Sbjct: 296 TKQVMRDPVTLENGQTYEREAIEKWFKECR---ESGRKLVCPLTLRELKSTEMNPSMALR 466
Query: 168 ------------AQLDQQKGVVDS------LVSNTRLLESKIQEARSKKDTLKARAQSAK 209
AQLD + +++ + R ++ Q +RS K ++ SA
Sbjct: 467 NTIEEWTTRNEAAQLDMARRSLNTGSPEKETLQALRYVQHICQRSRSNKHVVR----SAG 634
Query: 210 TSTKVSEMLGNVNTSGALSAFEKMEEKVMTMESQAEALGQLTSDDLEGKFAMLESSSVDD 269
+ +ML + + A E + V + E L + + KF E S +
Sbjct: 635 LIPMIVDMLKSSSRKVRCRALETLRIVVEGDDENKELLAEGDTVRTVVKFLSHELSKERE 814
Query: 270 DLANLKKELSGS 281
+ +L ELS S
Sbjct: 815 EAVSLLYELSKS 850
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.309 0.123 0.316
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,573,632
Number of Sequences: 36976
Number of extensions: 57284
Number of successful extensions: 417
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 409
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 414
length of query: 319
length of database: 9,014,727
effective HSP length: 96
effective length of query: 223
effective length of database: 5,465,031
effective search space: 1218701913
effective search space used: 1218701913
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC137825.9