BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137823.12 + phase: 0 /pseudo
(59 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC76765 homologue to GP|12054977|emb|CAC20725. putative chalcone... 120 8e-29
TC85146 homologue to SP|P30074|CHS2_MEDSA Chalcone synthase 2 (E... 120 8e-29
TC76766 SP|P30075|CHS4_MEDSA Chalcone synthase 4 (EC 2.3.1.74) (... 120 8e-29
TC85138 homologue to SP|P30076|CHS8_MEDSA Chalcone synthase 8 (E... 119 3e-28
TC76769 homologue to SP|P30075|CHS4_MEDSA Chalcone synthase 4 (E... 119 3e-28
TC76767 homologue to SP|P30074|CHS2_MEDSA Chalcone synthase 2 (E... 119 3e-28
TC85186 homologue to SP|P30075|CHS4_MEDSA Chalcone synthase 4 (E... 119 3e-28
TC85150 homologue to SP|P30077|CHS9_MEDSA Chalcone synthase 9 (E... 119 3e-28
TC76768 SP|P30075|CHS4_MEDSA Chalcone synthase 4 (EC 2.3.1.74) (... 119 3e-28
TC85169 SP|P30077|CHS9_MEDSA Chalcone synthase 9 (EC 2.3.1.74) (... 118 5e-28
AW684295 homologue to SP|P30077|CHS9_ Chalcone synthase 9 (EC 2.... 118 5e-28
TC85174 homologue to SP|P30075|CHS4_MEDSA Chalcone synthase 4 (E... 117 7e-28
TC79835 homologue to SP|P51082|CHSB_PEA Chalcone synthase 1B (EC... 105 4e-24
TC83930 homologue to SP|P51081|CHSA_PEA Chalcone synthase 1A (EC... 100 1e-22
TC85145 similar to GP|3288721|dbj|BAA31259.1 chalcone synthase {... 95 5e-21
BQ135372 similar to GP|12054977|em putative chalcone synthase {M... 81 7e-17
TC79323 similar to GP|18376655|dbj|BAB84112. chalcone synthase {... 77 1e-15
BQ135497 similar to GP|12054977|emb putative chalcone synthase {... 65 7e-12
BQ135501 weakly similar to GP|12054977|emb putative chalcone syn... 61 8e-11
BQ135488 weakly similar to GP|12054977|emb putative chalcone syn... 60 1e-10
>TC76765 homologue to GP|12054977|emb|CAC20725. putative chalcone synthase
{Medicago truncatula}, complete
Length = 1418
Score = 120 bits (302), Expect = 8e-29
Identities = 59/59 (100%), Positives = 59/59 (100%)
Frame = +2
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM
Sbjct: 125 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 301
>TC85146 homologue to SP|P30074|CHS2_MEDSA Chalcone synthase 2 (EC 2.3.1.74)
(Naringenin-chalcone synthase 2). [Alfalfa] {Medicago
sativa}, complete
Length = 1406
Score = 120 bits (302), Expect = 8e-29
Identities = 59/59 (100%), Positives = 59/59 (100%)
Frame = +3
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM
Sbjct: 114 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 290
>TC76766 SP|P30075|CHS4_MEDSA Chalcone synthase 4 (EC 2.3.1.74)
(Naringenin-chalcone synthase 4) (CHS12-1). [Alfalfa],
partial (50%)
Length = 731
Score = 120 bits (302), Expect = 8e-29
Identities = 59/59 (100%), Positives = 59/59 (100%)
Frame = +1
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM
Sbjct: 118 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 294
>TC85138 homologue to SP|P30076|CHS8_MEDSA Chalcone synthase 8 (EC 2.3.1.74)
(Naringenin-chalcone synthase 8). [Alfalfa] {Medicago
sativa}, complete
Length = 1479
Score = 119 bits (297), Expect = 3e-28
Identities = 58/59 (98%), Positives = 58/59 (98%)
Frame = +1
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MVSVSEIR AQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM
Sbjct: 82 MVSVSEIRNAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 258
>TC76769 homologue to SP|P30075|CHS4_MEDSA Chalcone synthase 4 (EC 2.3.1.74)
(Naringenin-chalcone synthase 4) (CHS12-1). [Alfalfa],
partial (33%)
Length = 814
Score = 119 bits (297), Expect = 3e-28
Identities = 58/59 (98%), Positives = 58/59 (98%)
Frame = +1
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHK ELKEKFQRM
Sbjct: 100 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKVELKEKFQRM 276
>TC76767 homologue to SP|P30074|CHS2_MEDSA Chalcone synthase 2 (EC 2.3.1.74)
(Naringenin-chalcone synthase 2). [Alfalfa] {Medicago
sativa}, complete
Length = 1436
Score = 119 bits (297), Expect = 3e-28
Identities = 58/59 (98%), Positives = 58/59 (98%)
Frame = +2
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MVSVSEIR AQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM
Sbjct: 128 MVSVSEIRNAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 304
>TC85186 homologue to SP|P30075|CHS4_MEDSA Chalcone synthase 4 (EC 2.3.1.74)
(Naringenin-chalcone synthase 4) (CHS12-1). [Alfalfa],
partial (31%)
Length = 683
Score = 119 bits (297), Expect = 3e-28
Identities = 58/59 (98%), Positives = 58/59 (98%)
Frame = +2
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHK ELKEKFQRM
Sbjct: 104 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKVELKEKFQRM 280
>TC85150 homologue to SP|P30077|CHS9_MEDSA Chalcone synthase 9 (EC 2.3.1.74)
(Naringenin-chalcone synthase 9). [Alfalfa] {Medicago
sativa}, complete
Length = 1389
Score = 119 bits (297), Expect = 3e-28
Identities = 58/59 (98%), Positives = 58/59 (98%)
Frame = +3
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHK ELKEKFQRM
Sbjct: 114 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKVELKEKFQRM 290
>TC76768 SP|P30075|CHS4_MEDSA Chalcone synthase 4 (EC 2.3.1.74)
(Naringenin-chalcone synthase 4) (CHS12-1). [Alfalfa],
partial (52%)
Length = 717
Score = 119 bits (297), Expect = 3e-28
Identities = 58/59 (98%), Positives = 58/59 (98%)
Frame = +3
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHK ELKEKFQRM
Sbjct: 108 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKVELKEKFQRM 284
>TC85169 SP|P30077|CHS9_MEDSA Chalcone synthase 9 (EC 2.3.1.74)
(Naringenin-chalcone synthase 9). [Alfalfa] {Medicago
sativa}, complete
Length = 1612
Score = 118 bits (295), Expect = 5e-28
Identities = 57/59 (96%), Positives = 58/59 (97%)
Frame = +2
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MVSVSEIRKAQRAEGPATI+AIGTANPANCVEQSTYPDFYFKITNSEHK ELKEKFQRM
Sbjct: 311 MVSVSEIRKAQRAEGPATIMAIGTANPANCVEQSTYPDFYFKITNSEHKVELKEKFQRM 487
>AW684295 homologue to SP|P30077|CHS9_ Chalcone synthase 9 (EC 2.3.1.74)
(Naringenin-chalcone synthase 9). [Alfalfa] {Medicago
sativa}, partial (23%)
Length = 459
Score = 118 bits (295), Expect = 5e-28
Identities = 57/59 (96%), Positives = 58/59 (97%)
Frame = +3
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MVSVSEIRKAQRAEGPATI+AIGTANPANCVEQSTYPDFYFKITNSEHK ELKEKFQRM
Sbjct: 54 MVSVSEIRKAQRAEGPATIMAIGTANPANCVEQSTYPDFYFKITNSEHKVELKEKFQRM 230
>TC85174 homologue to SP|P30075|CHS4_MEDSA Chalcone synthase 4 (EC 2.3.1.74)
(Naringenin-chalcone synthase 4) (CHS12-1). [Alfalfa],
partial (82%)
Length = 1072
Score = 117 bits (294), Expect = 7e-28
Identities = 57/59 (96%), Positives = 58/59 (97%)
Frame = +1
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MVSVSEIRKAQRAEGPATILAIGTANP NCVEQSTYPDFYF+ITNSEHKTELKEKFQRM
Sbjct: 103 MVSVSEIRKAQRAEGPATILAIGTANPPNCVEQSTYPDFYFRITNSEHKTELKEKFQRM 279
>TC79835 homologue to SP|P51082|CHSB_PEA Chalcone synthase 1B (EC 2.3.1.74)
(Naringenin-chalcone synthase 1B). [Garden pea] {Pisum
sativum}, complete
Length = 1393
Score = 105 bits (262), Expect = 4e-24
Identities = 48/59 (81%), Positives = 55/59 (92%)
Frame = +2
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MV+ EIR+AQRA+GPAT+LAIGTA P NCV+QSTYPD+YF+ITNSEHKTELKEKFQRM
Sbjct: 122 MVTFDEIRQAQRADGPATVLAIGTATPQNCVDQSTYPDYYFRITNSEHKTELKEKFQRM 298
>TC83930 homologue to SP|P51081|CHSA_PEA Chalcone synthase 1A (EC 2.3.1.74)
(Naringenin-chalcone synthase 1A). [Garden pea] {Pisum
sativum}, partial (51%)
Length = 670
Score = 100 bits (249), Expect = 1e-22
Identities = 45/52 (86%), Positives = 50/52 (95%)
Frame = +2
Query: 8 RKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
R+AQRAEGPAT+ AIGTANP NCV+QSTYPDFYF+ITNSEHKTEL+EKFQRM
Sbjct: 71 RQAQRAEGPATVFAIGTANPQNCVDQSTYPDFYFRITNSEHKTELREKFQRM 226
>TC85145 similar to GP|3288721|dbj|BAA31259.1 chalcone synthase {Vitis
vinifera}, partial (98%)
Length = 1526
Score = 95.1 bits (235), Expect = 5e-21
Identities = 43/59 (72%), Positives = 52/59 (87%)
Frame = +3
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
MV+V EIRKAQR+ GPATILA GTA P++CV Q+ YPD+YF+ITNSEH T+LKEKF+RM
Sbjct: 117 MVTVEEIRKAQRSNGPATILAFGTATPSHCVTQAEYPDYYFRITNSEHMTDLKEKFKRM 293
>BQ135372 similar to GP|12054977|em putative chalcone synthase {Medicago
truncatula}, partial (26%)
Length = 710
Score = 81.3 bits (199), Expect = 7e-17
Identities = 40/50 (80%), Positives = 41/50 (82%)
Frame = +2
Query: 10 AQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
AQRAEGPATILAI TA P C +QSTY D YFKITNSEHKTELKEKF M
Sbjct: 110 AQRAEGPATILAIDTAYPTKCDQQSTYSDSYFKITNSEHKTELKEKFHPM 259
>TC79323 similar to GP|18376655|dbj|BAB84112. chalcone synthase {Vitis
vinifera}, partial (95%)
Length = 1418
Score = 77.0 bits (188), Expect = 1e-15
Identities = 34/59 (57%), Positives = 45/59 (75%)
Frame = +1
Query: 1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
M + IR++QRA GPATILAIGTA P NC+ QS + D+YF++T SEH T+LK K +R+
Sbjct: 40 MAYLEGIRESQRARGPATILAIGTATPVNCIYQSDFTDYYFRVTKSEHMTQLKAKLKRI 216
>BQ135497 similar to GP|12054977|emb putative chalcone synthase {Medicago
truncatula}, partial (13%)
Length = 753
Score = 64.7 bits (156), Expect = 7e-12
Identities = 29/44 (65%), Positives = 36/44 (80%)
Frame = +2
Query: 16 PATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
P TIL+IG A+PANCVEQS Y FYFKIT+ +H +EL++KF RM
Sbjct: 155 PVTILSIGAAHPANCVEQSPYAPFYFKITDKDHISELEKKFLRM 286
>BQ135501 weakly similar to GP|12054977|emb putative chalcone synthase
{Medicago truncatula}, partial (13%)
Length = 784
Score = 61.2 bits (147), Expect = 8e-11
Identities = 27/44 (61%), Positives = 34/44 (76%)
Frame = +1
Query: 16 PATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
P TIL+IG +PANC+EQS Y FYFKIT +H +EL++KF RM
Sbjct: 154 PPTILSIGAVHPANCMEQSPYAPFYFKITGKDHISELEKKFLRM 285
>BQ135488 weakly similar to GP|12054977|emb putative chalcone synthase
{Medicago truncatula}, partial (11%)
Length = 767
Score = 60.5 bits (145), Expect = 1e-10
Identities = 28/44 (63%), Positives = 35/44 (78%)
Frame = +2
Query: 16 PATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRM 59
PATILAIG A+PANC+EQ+ Y FYFKIT +H +EL++KF M
Sbjct: 155 PATILAIGAAHPANCMEQTPYAAFYFKITCKDHISELEKKFLGM 286
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.314 0.127 0.352
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,410,014
Number of Sequences: 36976
Number of extensions: 10759
Number of successful extensions: 92
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 92
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 92
length of query: 59
length of database: 9,014,727
effective HSP length: 35
effective length of query: 24
effective length of database: 7,720,567
effective search space: 185293608
effective search space used: 185293608
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)
Medicago: description of AC137823.12