BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137821.7 - phase: 0
(821 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC88319 similar to GP|19387174|gb|AAL87123.1 SEC10 {Arabidopsis ... 741 0.0
BF650252 similar to GP|14586367|em putative protein {Arabidopsis... 214 4e-75
TC89256 similar to PIR|T47525|T47525 kinesin-related protein-lik... 35 0.089
TC83868 31 2.2
TC82826 similar to GP|9759211|dbj|BAB09653.1 gene_id:MXC17.10~un... 30 2.9
TC88084 similar to GP|13605619|gb|AAK32803.1 AT3g49260/F2K15_120... 30 2.9
AW690313 weakly similar to GP|9802564|gb| F22O13.25 {Arabidopsis... 30 3.8
TC85362 weakly similar to PIR|T45863|T45863 hypothetical protein... 30 4.9
TC77822 weakly similar to GP|21536834|gb|AAM61166.1 unknown {Ara... 29 6.4
BQ751040 similar to GP|21751093|dbj unnamed protein product {Hom... 29 8.4
>TC88319 similar to GP|19387174|gb|AAL87123.1 SEC10 {Arabidopsis thaliana},
partial (52%)
Length = 1313
Score = 741 bits (1913), Expect(2) = 0.0
Identities = 390/390 (100%), Positives = 390/390 (100%)
Frame = +3
Query: 301 PMFIDVEVMNADTRLVLGDQAAQSSPNNVARGLSSLYKEITDTVRKEAATITAVFPSPNE 360
PMFIDVEVMNADTRLVLGDQAAQSSPNNVARGLSSLYKEITDTVRKEAATITAVFPSPNE
Sbjct: 3 PMFIDVEVMNADTRLVLGDQAAQSSPNNVARGLSSLYKEITDTVRKEAATITAVFPSPNE 182
Query: 361 VMSILVQRVLEQRVTALLDKLLVKPSLVNLPSMEEGGLLFYLRMLAVSYEKTQEIARDLR 420
VMSILVQRVLEQRVTALLDKLLVKPSLVNLPSMEEGGLLFYLRMLAVSYEKTQEIARDLR
Sbjct: 183 VMSILVQRVLEQRVTALLDKLLVKPSLVNLPSMEEGGLLFYLRMLAVSYEKTQEIARDLR 362
Query: 421 TVGCGDLDVEGLTESLFSSHKDEYPEYEQASLRQLYKVKMEELRAESQISDSSGTIGRSK 480
TVGCGDLDVEGLTESLFSSHKDEYPEYEQASLRQLYKVKMEELRAESQISDSSGTIGRSK
Sbjct: 363 TVGCGDLDVEGLTESLFSSHKDEYPEYEQASLRQLYKVKMEELRAESQISDSSGTIGRSK 542
Query: 481 GATVASSQQQISVTVVTEFVRWNEEAITRCNLFSSQPSTLATLVKAVFTCLLDQVSQYIA 540
GATVASSQQQISVTVVTEFVRWNEEAITRCNLFSSQPSTLATLVKAVFTCLLDQVSQYIA
Sbjct: 543 GATVASSQQQISVTVVTEFVRWNEEAITRCNLFSSQPSTLATLVKAVFTCLLDQVSQYIA 722
Query: 541 EGLERARDGLTEAANLRERFVLGTSVSRRVAAAAASAAEAAAAAGESSFRSFMVAVQRSG 600
EGLERARDGLTEAANLRERFVLGTSVSRRVAAAAASAAEAAAAAGESSFRSFMVAVQRSG
Sbjct: 723 EGLERARDGLTEAANLRERFVLGTSVSRRVAAAAASAAEAAAAAGESSFRSFMVAVQRSG 902
Query: 601 SSVAIIQQYFSNSISRLLLPVDGAHAAACEEMATAMSSAEAAAYKGLQQCIETVMAEVER 660
SSVAIIQQYFSNSISRLLLPVDGAHAAACEEMATAMSSAEAAAYKGLQQCIETVMAEVER
Sbjct: 903 SSVAIIQQYFSNSISRLLLPVDGAHAAACEEMATAMSSAEAAAYKGLQQCIETVMAEVER 1082
Query: 661 LLSAEQKATDYKSPDDGMAPDHRPTNACTR 690
LLSAEQKATDYKSPDDGMAPDHRPTNACTR
Sbjct: 1083LLSAEQKATDYKSPDDGMAPDHRPTNACTR 1172
Score = 92.4 bits (228), Expect(2) = 0.0
Identities = 45/46 (97%), Positives = 45/46 (97%)
Frame = +1
Query: 691 VVAYLSRVLESAFTALEGLNKQAFLSELGNRLHKVLLNHWQKYTFN 736
VVAYLSRVLESAFTALEGLNKQAFLSELGNRLHK LLNHWQKYTFN
Sbjct: 1174 VVAYLSRVLESAFTALEGLNKQAFLSELGNRLHKGLLNHWQKYTFN 1311
>BF650252 similar to GP|14586367|em putative protein {Arabidopsis thaliana},
partial (17%)
Length = 476
Score = 214 bits (544), Expect(2) = 4e-75
Identities = 109/112 (97%), Positives = 109/112 (97%)
Frame = +1
Query: 401 YLRMLAVSYEKTQEIARDLRTVGCGDLDVEGLTESLFSSHKDEYPEYEQASLRQLYKVKM 460
YLRMLAVSYEKTQEIARDLRTVGCGDLDVEGLTESLFSSHKDEYPEYEQASLRQLYKVKM
Sbjct: 139 YLRMLAVSYEKTQEIARDLRTVGCGDLDVEGLTESLFSSHKDEYPEYEQASLRQLYKVKM 318
Query: 461 EELRAESQISDSSGTIGRSKGATVASSQQQISVTVVTEFVRWNEEAITRCNL 512
EELRAESQISDSSGTIGRSKGATVASS QQISVTVVTEFVRWNE ITRCNL
Sbjct: 319 EELRAESQISDSSGTIGRSKGATVASSXQQISVTVVTEFVRWNEXXITRCNL 474
Score = 87.0 bits (214), Expect(2) = 4e-75
Identities = 45/45 (100%), Positives = 45/45 (100%)
Frame = +3
Query: 351 ITAVFPSPNEVMSILVQRVLEQRVTALLDKLLVKPSLVNLPSMEE 395
ITAVFPSPNEVMSILVQRVLEQRVTALLDKLLVKPSLVNLPSMEE
Sbjct: 3 ITAVFPSPNEVMSILVQRVLEQRVTALLDKLLVKPSLVNLPSMEE 137
>TC89256 similar to PIR|T47525|T47525 kinesin-related protein-like -
Arabidopsis thaliana, partial (27%)
Length = 865
Score = 35.4 bits (80), Expect = 0.089
Identities = 39/205 (19%), Positives = 90/205 (43%), Gaps = 8/205 (3%)
Frame = +1
Query: 55 KLEDLEAEGADAVQNKYSQVATSPLFPEVEKLLSLFKDSCKELLELRKQIDGRLHNLKKD 114
+L+D+E + V K AT L V +L +++ K L L +++ +D
Sbjct: 268 QLKDMEKDMQSFVSTKSE--ATEDLRVRVVELKNMYGSGIKALDNLAEELKSNNQLTYED 441
Query: 115 VSVQDSKHRRTLAELEKGV--------DGLFASFARLDSRISSVGQTAAKIGDHLQSADA 166
+ + +KH L +L KG+ + L S + ++ +++ + H ++ +
Sbjct: 442 LKSEVAKHSSALEDLFKGIALEADSLLNDLQNSLHKQEANVTAFAHQQREA--HSRAVET 615
Query: 167 QRETASQTIELIKYLMEFNSSPGDLMELSPLFSDDSRVAEAASIAQKLRSFAEEDIGRHG 226
R + T++ + + SS ++E F +D ++ E ++ ++ E+ +
Sbjct: 616 TRSVSKITMKFFETIDRHASSLTQIVE-ETQFVNDQKLCELEKKFEECTAYEEKQL-LEK 789
Query: 227 ITAPSAVGNATASRGLEVAVANLQE 251
+ A NA + +++AV +L+E
Sbjct: 790 VAEMLASSNARKKKLVQMAVNDLRE 864
>TC83868
Length = 828
Score = 30.8 bits (68), Expect = 2.2
Identities = 24/76 (31%), Positives = 35/76 (45%), Gaps = 4/76 (5%)
Frame = +3
Query: 60 EAEGADAVQNKY----SQVATSPLFPEVEKLLSLFKDSCKELLELRKQIDGRLHNLKKDV 115
E E D +NK +V ++E L K+S E+ LR+Q+ KKD+
Sbjct: 129 EGEMVDVPENKKVKMDPEVEKDAYLKQIEALNQRLKESELEIESLREQLR------KKDL 290
Query: 116 SVQDSKHRRTLAELEK 131
V DSK + E+EK
Sbjct: 291 QVDDSKEVKVDHEIEK 338
>TC82826 similar to GP|9759211|dbj|BAB09653.1 gene_id:MXC17.10~unknown
protein {Arabidopsis thaliana}, partial (33%)
Length = 1191
Score = 30.4 bits (67), Expect = 2.9
Identities = 22/66 (33%), Positives = 31/66 (46%)
Frame = +1
Query: 563 GTSVSRRVAAAAASAAEAAAAAGESSFRSFMVAVQRSGSSVAIIQQYFSNSISRLLLPVD 622
G+S R AAAA AA AAAAAG ++ + + S++ + S+ PV
Sbjct: 682 GSSSKRAKEAAAAQAAAAAAAAGSAASVQVRIMLDDGTSNMV------TRSVGARSEPVI 843
Query: 623 GAHAAA 628
G H A
Sbjct: 844 GLHGGA 861
>TC88084 similar to GP|13605619|gb|AAK32803.1 AT3g49260/F2K15_120
{Arabidopsis thaliana}, partial (33%)
Length = 1706
Score = 30.4 bits (67), Expect = 2.9
Identities = 20/53 (37%), Positives = 29/53 (53%), Gaps = 4/53 (7%)
Frame = +1
Query: 571 AAAAASAAEAAAAAGESSFRSFMVAVQRSGSSV----AIIQQYFSNSISRLLL 619
AAA A+AAEAA AA +++ R +A S V +IQ Y+ ++R L
Sbjct: 442 AAATAAAAEAAVAAAQAAARVVRLAGHGRNSKVEKAAILIQSYYRGYLARRAL 600
>AW690313 weakly similar to GP|9802564|gb| F22O13.25 {Arabidopsis thaliana},
partial (25%)
Length = 654
Score = 30.0 bits (66), Expect = 3.8
Identities = 15/48 (31%), Positives = 26/48 (53%)
Frame = -3
Query: 753 FVRSFNAPSVDEKFELLGILANVFIVAPESLSTLFEGTPSIRKDAQRF 800
F+ SF + +D+ E+ LA+V + P+ T+F G+P + A F
Sbjct: 454 FITSFPSFEIDDGSEVH*TLASVTFLLPKETLTMFPGSPLLETSADFF 311
>TC85362 weakly similar to PIR|T45863|T45863 hypothetical protein F3A4.180 -
Arabidopsis thaliana, partial (29%)
Length = 1175
Score = 29.6 bits (65), Expect = 4.9
Identities = 18/56 (32%), Positives = 29/56 (51%)
Frame = +1
Query: 371 EQRVTALLDKLLVKPSLVNLPSMEEGGLLFYLRMLAVSYEKTQEIARDLRTVGCGD 426
EQR+ + + + P +LPS++EG L+ L+ A + T +A D V C D
Sbjct: 595 EQRLVQVTLQHPMYPMDYSLPSIDEGWLVINLKKKAKGIKSTTMVAVDCEMVLCED 762
>TC77822 weakly similar to GP|21536834|gb|AAM61166.1 unknown {Arabidopsis
thaliana}, partial (33%)
Length = 2391
Score = 29.3 bits (64), Expect = 6.4
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +2
Query: 103 QIDGRLHNLKKDVSVQDSKHRRTLAELEKGVDGL 136
Q + HN+ K+ + DS+HR T L +G+DG+
Sbjct: 983 QFPQKFHNISKN-DIYDSQHRSTYTVLWQGMDGI 1081
>BQ751040 similar to GP|21751093|dbj unnamed protein product {Homo sapiens},
partial (19%)
Length = 751
Score = 28.9 bits (63), Expect = 8.4
Identities = 22/46 (47%), Positives = 23/46 (49%)
Frame = -1
Query: 545 RARDGLTEAANLRERFVLGTSVSRRVAAAAASAAEAAAAAGESSFR 590
R R L + R R G S RR A A AA AAAAA SSFR
Sbjct: 691 RPRRALRRSTARRARRRSG-STPRRCPRARAPAAAAAAAAAASSFR 557
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.129 0.348
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,444,888
Number of Sequences: 36976
Number of extensions: 202049
Number of successful extensions: 1111
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1055
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1091
length of query: 821
length of database: 9,014,727
effective HSP length: 104
effective length of query: 717
effective length of database: 5,169,223
effective search space: 3706332891
effective search space used: 3706332891
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC137821.7