BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137821.2 + phase: 0 /pseudo
(428 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78089 similar to GP|4522009|gb|AAD21782.1| unknown protein {Ar... 226 1e-59
TC82747 similar to GP|9758202|dbj|BAB08676.1 emb|CAB82688.1~gene... 169 2e-42
BF004365 weakly similar to GP|21539547|gb| unknown protein {Arab... 166 2e-41
TC87842 similar to GP|9758120|dbj|BAB08592.1 gene_id:MCO15.18~pi... 73 5e-16
TC79421 similar to GP|11558256|emb|CAC17796. microtubule-associa... 33 0.28
TC89612 similar to GP|6934300|gb|AAF31706.1| unknown {Euphorbia ... 30 1.8
TC81279 similar to PIR|T47949|T47949 hypothetical protein F2A19.... 30 2.3
AJ501172 similar to GP|20466324|gb| unknown protein {Arabidopsis... 29 3.0
BI311165 similar to GP|16902294|d kinesin-related protein {Arabi... 28 5.2
CA858495 28 6.8
TC83917 similar to PIR|T47972|T47972 hypothetical protein F15G16... 28 6.8
TC86848 similar to GP|10177413|dbj|BAB10544. fibrillarin-like {A... 28 8.9
BI308178 weakly similar to GP|6714444|gb|A putative disease resi... 28 8.9
TC78850 homologue to GP|10177413|dbj|BAB10544. fibrillarin-like ... 28 8.9
TC93421 similar to GP|19912731|dbj|BAB88648. microtubule bundlin... 28 8.9
BQ151752 homologue to PIR|T09555|T09 fibrillarin - Arabidopsis t... 28 8.9
>TC78089 similar to GP|4522009|gb|AAD21782.1| unknown protein {Arabidopsis
thaliana}, partial (85%)
Length = 2853
Score = 226 bits (577), Expect = 1e-59
Identities = 154/472 (32%), Positives = 254/472 (53%), Gaps = 44/472 (9%)
Frame = +1
Query: 1 MLLQIEQEYSEIYHRKVEDTIKHKDYLNKVLDDFQSEIANIASSLGEDYVS----FSRGK 56
ML+++E+E ++Y RKV++ K ++ L ++EIA + ++LGE +S +
Sbjct: 796 MLMELERECLDVYRRKVDEAANIKARFHQSLAAKEAEIATLIAALGEHDMSSPIKMEKRS 975
Query: 57 GTLKQQLANIIPVVEDLRFKKKERVKEILEIKSQISQIRAEKAGCGQSKGVTDQ------ 110
+LK++LA+I P+VE+L+ KK+ER+K++ +IK+QI +I E G V D
Sbjct: 976 ASLKEKLASITPLVEELKKKKEERLKQLADIKTQIEKISGEICGI---HSVNDDVSVSST 1146
Query: 111 -DVDQCDLTMEKLGKLKSHLNELQNEKNIRHQKVKSHISTISELSAVMSIDISEILNGIH 169
VD+ DL++ +L + ++HL LQ EK+ R QKV ++ + L V+ +D + ++ +H
Sbjct: 1147 GGVDEQDLSLRRLNEYQTHLRSLQKEKSDRLQKVLQCVNEVHSLCGVLGLDFGQTVDDVH 1326
Query: 170 PSLNDSSNGAQQSISDETLARLNESVLLLKQEKQQSLQK----VQFLEELWDFMGITTDE 225
PSL+ + +IS+ TL L +++L LK E++ +QK V L ELW+ M + +E
Sbjct: 1327 PSLHGTQVEQSTNISNSTLEGLEKTILKLKTERKARIQKLKDIVANLFELWNLMDTSKEE 1506
Query: 226 QKAVCDDVIGLISASVDEVSIHGSLSNDIVKQVDVEVQRLQVLNPSKMKEFVFKRQKELA 285
+ + +++ S E++ G+LS +I+++ EV+RL L S+M KEL
Sbjct: 1507 RNYFLRN-NSIVTTSESEITERGALSTEIIEKASAEVERLAELKASRM--------KELV 1659
Query: 286 FKRQNELEEIHRGAHMDMDAKAARQILTDLI--GNIDMSELLQGMDKQIRKAKEQAQSRR 343
FK+++ELEEI R H++ D A + + LI G +D ELL ++ QI KAK++A SRR
Sbjct: 1660 FKKRSELEEICRLTHIEPDTSTAAEKASALIDSGLVDPCELLANIEAQIVKAKDEALSRR 1839
Query: 344 DIIDRVAEWKSAAEEEKWLDEYERFI*SVRRKRGFLSPRFHLW*RI*LQK*KHGRQTKKK 403
++ DR+ +W A EEE WLDEY + RG I L++ + R T K
Sbjct: 1840 EVTDRIDKWLFACEEENWLDEYSQDNNRYSAGRGA---------HINLKRAERARVTITK 1992
Query: 404 VSYM---------------------------KSLHEYNVQRQLREEEKRKSR 428
+ M + L +Y + RQ REEEKR+ R
Sbjct: 1993 IPAMVDNLISKTLAWEDEKKTCFLYDGVRLVELLDDYKLTRQQREEEKRRQR 2148
>TC82747 similar to GP|9758202|dbj|BAB08676.1
emb|CAB82688.1~gene_id:K17N15.15~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (45%)
Length = 1329
Score = 169 bits (427), Expect = 2e-42
Identities = 121/392 (30%), Positives = 200/392 (50%), Gaps = 29/392 (7%)
Frame = +3
Query: 66 IIPVVEDLRFKKKERVKEILEIKSQISQIRAEKAGCGQSKGVTDQDVDQCDLTMEKLGKL 125
I+P +E++ K R + E++ QI + E G K VD+ DL++ K L
Sbjct: 6 ILPQLEEMIIYKFYRPNQFTEVQEQIQSLSMEIYG---PKEYGPSVVDETDLSLRKFEDL 176
Query: 126 KSHLNELQNEKNIRHQKVKSHISTISELSAVMSIDISEILNGIHPSLNDSSNGAQQSISD 185
LN LQ+EK R + V+ H+ T++ L V+ D + + GIHPSL D +S+S+
Sbjct: 177 HRQLNALQSEKIGRLKTVQEHLCTLNSLCLVLGFDFKQTVLGIHPSLGDE---GPKSVSN 347
Query: 186 ETLARLNESVLLLKQEKQQSLQKVQFLE----ELWDFMGITTDEQKAVCDDVIGLISASV 241
+T+ +L ++ L++ K Q +QK+Q L ELW+ M +EQ+ + +V I+A
Sbjct: 348 DTIQQLAAAIQQLREIKLQRMQKLQDLATTMLELWNLMDTPIEEQQ-MFQNVTCNIAA*E 524
Query: 242 DEVSIHGSLSNDIVKQVDVEVQRLQVLNPSKMKEFVFKRQKELAFKRQNELEEIHRGAHM 301
DE++ +LS D + V+VEV RL+ L SKM KEL K++ ELEEI R H+
Sbjct: 525 DEITEPNTLSEDFINCVEVEVSRLEELKSSKM--------KELVLKKRTELEEICRKTHL 680
Query: 302 DMDAKAARQILTDLI--GNIDMSELLQGMDKQIRKAKEQAQSRRDIIDRVAEWKSAAEEE 359
+ A + + I G +D + +L+ ++Q+ + KE+A R+DI+++V +W +A EE
Sbjct: 681 VPEIDGAVEYAVEAIESGIVDPACVLEQFERQVAQVKEEALGRKDILEKVEKWLAACNEE 860
Query: 360 KWLDEYER-------------FI*SVRRKRGF----------LSPRFHLW*RI*LQK*KH 396
WL+EY R + + RG L+ + W +K K
Sbjct: 861 SWLEEYNRDDNRYNAGRGAHLTLKRAEKARGLVNKIPGMVDALTSKTIAW-----EKEKG 1025
Query: 397 GRQTKKKVSYMKSLHEYNVQRQLREEEKRKSR 428
T V + + EYN+ R+ +E+E+R+ R
Sbjct: 1026FEFTYDGVRLLSMIEEYNILREEKEQERRRQR 1121
>BF004365 weakly similar to GP|21539547|gb| unknown protein {Arabidopsis
thaliana}, partial (6%)
Length = 649
Score = 166 bits (419), Expect = 2e-41
Identities = 93/137 (67%), Positives = 101/137 (72%), Gaps = 7/137 (5%)
Frame = +1
Query: 299 AHMDMDAKAARQILTDLIGNIDMSELLQGMDKQIRKAKEQAQSRRDIIDRVAEWKSAAEE 358
AHMDMDAKAARQILTDLIGNIDMSELLQGMDKQIRKAKEQAQSRRDIIDRVAEWKSAAEE
Sbjct: 1 AHMDMDAKAARQILTDLIGNIDMSELLQGMDKQIRKAKEQAQSRRDIIDRVAEWKSAAEE 180
Query: 359 EKWLDEYER-------FI*SVRRKRGFLSPRFHLW*RI*LQK*KHGRQTKKKVSYMKSLH 411
EKWLDEYER + + L+ + W + + +KV + SLH
Sbjct: 181 EKWLDEYERRAEKARILVTQIPSMVENLTTKVKTW-----ETNEEKSFLYEKVPLLNSLH 345
Query: 412 EYNVQRQLREEEKRKSR 428
EYNVQRQLREEEKRKSR
Sbjct: 346 EYNVQRQLREEEKRKSR 396
>TC87842 similar to GP|9758120|dbj|BAB08592.1
gene_id:MCO15.18~pir||T04799~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (31%)
Length = 835
Score = 72.8 bits (177), Expect(2) = 5e-16
Identities = 41/102 (40%), Positives = 63/102 (61%), Gaps = 2/102 (1%)
Frame = +2
Query: 1 MLLQIEQEYSEIYHRKVEDTIKHKDYLNKVLDDFQSEIANIASSLGEDYVSF--SRGKGT 58
MLLQ+EQE ++Y RKVE K + L + L D + E++ + S+LGE + GT
Sbjct: 371 MLLQLEQECLDVYKRKVEHAAKSRAQLLQALSDAKLELSTLLSALGEKGFAGIPDNTSGT 550
Query: 59 LKQQLANIIPVVEDLRFKKKERVKEILEIKSQISQIRAEKAG 100
+K+QLA I P +E L +K+ER+K+ +++SQI +I E G
Sbjct: 551 IKEQLAAIAPALEQLWQQKEERIKDFSDVQSQIQKICGEITG 676
Score = 29.3 bits (64), Expect(2) = 5e-16
Identities = 14/34 (41%), Positives = 22/34 (64%)
Frame = +1
Query: 120 EKLGKLKSHLNELQNEKNIRHQKVKSHISTISEL 153
+K + +S L ELQ EK+ R QKV ++T+ +L
Sbjct: 730 KKFEEYQSELQELQKEKSDRLQKVFEFVNTVHDL 831
>TC79421 similar to GP|11558256|emb|CAC17796. microtubule-associated protein
MAP65-1c {Nicotiana tabacum}, partial (30%)
Length = 1030
Score = 32.7 bits (73), Expect = 0.28
Identities = 23/62 (37%), Positives = 31/62 (49%), Gaps = 6/62 (9%)
Frame = +3
Query: 373 RRKRGFLSPRFHLW*RI*LQK*KHGRQTKKK------VSYMKSLHEYNVQRQLREEEKRK 426
+RK G+ +F LW L+K HG +T V + L EY + R REEEKR+
Sbjct: 111 QRKLGYW*IKFPLWLIHWLRKHVHGEETHDMSFTYDGVPLLAMLDEYAMLRHEREEEKRR 290
Query: 427 SR 428
R
Sbjct: 291 MR 296
>TC89612 similar to GP|6934300|gb|AAF31706.1| unknown {Euphorbia esula},
complete
Length = 1504
Score = 30.0 bits (66), Expect = 1.8
Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 4/45 (8%)
Frame = +3
Query: 133 QNEKN----IRHQKVKSHISTISELSAVMSIDISEILNGIHPSLN 173
Q KN +RH+K H+S+ S LS +S I+N PSLN
Sbjct: 30 QTNKNCSYLLRHKKKT*HLSSSSSLSFTISNTSQSIINNTIPSLN 164
>TC81279 similar to PIR|T47949|T47949 hypothetical protein F2A19.170 -
Arabidopsis thaliana, partial (31%)
Length = 1205
Score = 29.6 bits (65), Expect = 2.3
Identities = 19/85 (22%), Positives = 42/85 (49%), Gaps = 5/85 (5%)
Frame = +2
Query: 116 DLTMEKLGKLKSHLNELQNEKNIRHQKVKSHISTISELSAVMSIDISEILNGIHPSLND- 174
D +++L +LK HL E +NE++ + + I + + + + IS + + + +D
Sbjct: 449 DKAVQELTRLKQHLLEKENEESEKMDEDTKVIEELRDSNNYLRAQISHLERALEQATSDQ 628
Query: 175 ----SSNGAQQSISDETLARLNESV 195
S+N ++ S E + LN+ +
Sbjct: 629 EKLKSANNSEILTSREVIDDLNKKL 703
>AJ501172 similar to GP|20466324|gb| unknown protein {Arabidopsis thaliana},
partial (21%)
Length = 653
Score = 29.3 bits (64), Expect = 3.0
Identities = 25/101 (24%), Positives = 42/101 (40%)
Frame = +3
Query: 121 KLGKLKSHLNELQNEKNIRHQKVKSHISTISELSAVMSIDISEILNGIHPSLNDSSNGAQ 180
K+ H N+++N K + + + ++ + A +E+ HP NDS A
Sbjct: 297 KINSKNQHKNKVRN-KAVMEALIARLFAGVTTIKA----SYAELQMAQHPYNNDSIQAAD 461
Query: 181 QSISDETLARLNESVLLLKQEKQQSLQKVQFLEELWDFMGI 221
Q++ DE A LK+E S Q L E+ + I
Sbjct: 462 QAVVDELRAISELKRRFLKKELDLSPQVTIMLAEIQEQQSI 584
>BI311165 similar to GP|16902294|d kinesin-related protein {Arabidopsis
thaliana}, partial (16%)
Length = 657
Score = 28.5 bits (62), Expect = 5.2
Identities = 36/183 (19%), Positives = 77/183 (41%), Gaps = 5/183 (2%)
Frame = +2
Query: 116 DLTMEKLGKLKSHLNELQNEKNIRHQKVKSHISTISELSAVMSIDISEILNGIHPSLNDS 175
++ EKL + HL+E + +++QK+ S EL++ ++++ + +
Sbjct: 17 EIENEKLKLEQVHLSEDNSGLRVQNQKLSEEASYAKELASAAAVELKNLAGEV------- 175
Query: 176 SNGAQQSISDETLARLNESVLLLKQEKQQSLQKVQFLEEL-WDFMGITTDEQKAVCDDVI 234
+ S+ + L E L+ ++ S VQ + + + + + +
Sbjct: 176 ---TKLSLQNAKL----EKELMTVRDLANSRVAVQMVNGVNRKYSDARSGRKGRISSRAN 334
Query: 235 GLISASVDEVSIHGSLSNDIVKQVDVEVQRLQVLNPS-KMKEFV---FKRQKELAFKRQN 290
L A +D+ ++D+ ++ QR L + KEFV F+++ E A KR+
Sbjct: 335 DLSGAGLDDFDSWSLDADDLRLELQARKQREAALESALSEKEFVEEEFRKKAEEAKKREE 514
Query: 291 ELE 293
LE
Sbjct: 515 ALE 523
>CA858495
Length = 763
Score = 28.1 bits (61), Expect = 6.8
Identities = 28/101 (27%), Positives = 46/101 (44%), Gaps = 5/101 (4%)
Frame = +3
Query: 113 DQCDLTMEKLGKLKSHLNELQNEKNIRHQKV---KSHISTISELSAVMSIDISEILNGIH 169
++ +L + + L +N L NEK QKV +S++S+ SE A S+D + +L
Sbjct: 63 EKTNLLLNEKAGLVEKVNMLLNEKEGLEQKVNILESNLSSFSEKEA-GSVDTTNLLLKEK 239
Query: 170 PSLNDSSNGAQQSIS--DETLARLNESVLLLKQEKQQSLQK 208
L N + ++S E L + L+ E LQK
Sbjct: 240 EGLEQKLNILESNLSSFSEKETGLEMRIAQLQSETNSLLQK 362
>TC83917 similar to PIR|T47972|T47972 hypothetical protein F15G16.190 -
Arabidopsis thaliana, partial (29%)
Length = 657
Score = 28.1 bits (61), Expect = 6.8
Identities = 23/61 (37%), Positives = 32/61 (51%), Gaps = 8/61 (13%)
Frame = +2
Query: 45 LGEDYVSFSRGKGTLKQQLANIIPVVEDLRFKKKE---RVKEIL-----EIKSQISQIRA 96
LG DY+ K TLK + NI +E +R ++KE R KEIL +K + S I+
Sbjct: 464 LGYDYL-----KNTLKLKFPNIQANLERIRQERKEREMRSKEILLNKYESLKEKFSSIKG 628
Query: 97 E 97
E
Sbjct: 629 E 631
>TC86848 similar to GP|10177413|dbj|BAB10544. fibrillarin-like {Arabidopsis
thaliana}, partial (92%)
Length = 1250
Score = 27.7 bits (60), Expect = 8.9
Identities = 18/61 (29%), Positives = 28/61 (45%), Gaps = 5/61 (8%)
Frame = +3
Query: 26 YLNKVLDDFQSEIANIASSLGEDY-VSFSRGKG----TLKQQLANIIPVVEDLRFKKKER 80
YL S +++I G Y V FS G + ++ N+IP++ED R K R
Sbjct: 519 YLGAASGTTVSHVSDIVGPTGVVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYR 698
Query: 81 V 81
+
Sbjct: 699 M 701
>BI308178 weakly similar to GP|6714444|gb|A putative disease resistance
protein {Arabidopsis thaliana}, partial (7%)
Length = 602
Score = 27.7 bits (60), Expect = 8.9
Identities = 25/87 (28%), Positives = 42/87 (47%), Gaps = 6/87 (6%)
Frame = +2
Query: 97 EKAGCGQSKG-VTDQDVDQCDLTMEKLGKLKSHLNELQN--EKNIRHQKVKSHI-STISE 152
E C G VT D++ D T GKL S + ELQ+ N+ + + I I
Sbjct: 197 EGISCDNLTGHVTSLDLEAFDYTKALRGKLDSSICELQHLTSLNLHGNQFEGKIPKCIGS 376
Query: 153 LSAVMSIDI--SEILNGIHPSLNDSSN 177
L ++ +++ + +++ I PSL + SN
Sbjct: 377 LDKLIELNLHDNNLVSVIPPSLGNLSN 457
>TC78850 homologue to GP|10177413|dbj|BAB10544. fibrillarin-like
{Arabidopsis thaliana}, partial (87%)
Length = 1177
Score = 27.7 bits (60), Expect = 8.9
Identities = 18/61 (29%), Positives = 28/61 (45%), Gaps = 5/61 (8%)
Frame = +3
Query: 26 YLNKVLDDFQSEIANIASSLGEDY-VSFSRGKG----TLKQQLANIIPVVEDLRFKKKER 80
YL S +++I G Y V FS G + ++ N+IP++ED R K R
Sbjct: 498 YLGAASGTTVSHVSDIVGPTGVVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYR 677
Query: 81 V 81
+
Sbjct: 678 M 680
>TC93421 similar to GP|19912731|dbj|BAB88648. microtubule bundling
polypeptide TMBP200 {Nicotiana tabacum}, partial (6%)
Length = 389
Score = 27.7 bits (60), Expect = 8.9
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +3
Query: 213 EELWDFMGITTDEQKAVCDD 232
E++W F+G TD QK++ DD
Sbjct: 291 EDIWRFVGKLTDAQKSMLDD 350
>BQ151752 homologue to PIR|T09555|T09 fibrillarin - Arabidopsis thaliana,
partial (53%)
Length = 682
Score = 27.7 bits (60), Expect = 8.9
Identities = 18/61 (29%), Positives = 28/61 (45%), Gaps = 5/61 (8%)
Frame = +1
Query: 26 YLNKVLDDFQSEIANIASSLGEDY-VSFSRGKG----TLKQQLANIIPVVEDLRFKKKER 80
YL S +++I G Y V FS G + ++ N+IP++ED R K R
Sbjct: 85 YLGAASGTTVSHVSDIVGPTGVVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYR 264
Query: 81 V 81
+
Sbjct: 265 M 267
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.135 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,558,258
Number of Sequences: 36976
Number of extensions: 100982
Number of successful extensions: 639
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 624
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 630
length of query: 428
length of database: 9,014,727
effective HSP length: 99
effective length of query: 329
effective length of database: 5,354,103
effective search space: 1761499887
effective search space used: 1761499887
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC137821.2