BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137669.1 - phase: 0
(284 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG585571 weakly similar to GP|13235641|em S-adenosyl-L-methionin... 291 3e-79
TC86418 weakly similar to GP|18461100|dbj|BAB84353. S-adenosyl-L... 246 7e-66
AW584129 weakly similar to GP|13235641|em S-adenosyl-L-methionin... 221 3e-58
TC89843 similar to PIR|E85430|E85430 hypothetical protein AT4g36... 174 8e-50
BI271770 weakly similar to GP|13235641|em S-adenosyl-L-methionin... 141 3e-34
BQ123896 weakly similar to PIR|A86285|A86 protein F9L1.6 [import... 120 5e-28
AL365973 similar to GP|13235641|emb S-adenosyl-L-methionine:sali... 98 4e-21
BI271462 similar to GP|6651395|gb|A floral nectary-specific prot... 79 4e-17
TC83132 similar to GP|21593254|gb|AAM65203.1 S-adenosyl-L-methio... 74 5e-14
TC86419 weakly similar to GP|18461100|dbj|BAB84353. S-adenosyl-L... 64 5e-11
TC85117 similar to GP|21593254|gb|AAM65203.1 S-adenosyl-L-methio... 64 9e-11
BM815618 weakly similar to GP|6651395|gb|A floral nectary-specif... 62 2e-10
TC76387 homologue to SP|Q40468|IF4Z_TOBAC Eukaryotic initiation ... 28 5.2
TC93600 27 6.8
TC90734 similar to GP|12322994|gb|AAG51484.1 unknown protein {Ar... 27 6.8
TC87454 similar to SP|P49730|RIR2_TOBAC Ribonucleoside-diphospha... 27 6.8
NP212723 NP212723|AJ132893.1|CAB85497.1 H+-ATPase 27 8.9
CA920867 similar to GP|10798819|dbj putative damage-specific DNA... 27 8.9
>BG585571 weakly similar to GP|13235641|em S-adenosyl-L-methionine:salicylic
acid carboxyl methyltransferase {Stephanotis
floribunda}, partial (54%)
Length = 723
Score = 291 bits (744), Expect = 3e-79
Identities = 134/214 (62%), Positives = 174/214 (80%)
Frame = +2
Query: 17 LHMNGGIGETSYSNNSLLQKKVISLTKEMRDEAIKNLYCKTFPKRLGIADLGCSSGPNTL 76
+HMNGG G+TSY+NNS Q K ISLTK +R+EAI +LY T P+ L IADLGCS G NTL
Sbjct: 2 VHMNGGDGKTSYANNSFFQGKGISLTKHIREEAITSLYSSTLPRSLAIADLGCSCGQNTL 181
Query: 77 LVISEVIKLVDKLCQEHNHESPEYQVFMNDLQGNDFNNIFRLLDRFTEKLNDEVEDGIGG 136
V+SE+I +V+KLCQ+ + SPEY++F NDL GNDFNNIF+ LD F KL DE++ +
Sbjct: 182 SVVSEIIMVVEKLCQQLKYASPEYKIFFNDLSGNDFNNIFKSLDSFKHKLLDEIKTEM-S 358
Query: 137 PIFFYGAPGSFYGRIFPTKTMHFIHSSYSLQWLSQVPKGVENNKGNIYMATTSPANVLNA 196
P +F+G PGSFY R+FP +++HF+H SYSL WLS+VP+G++NNKGNIY++ TSP+NV+ A
Sbjct: 359 PCYFFGVPGSFYDRVFPDRSLHFVHCSYSLHWLSKVPEGIDNNKGNIYISDTSPSNVVKA 538
Query: 197 YHEQFQRDFSLFLKCRAEELVDGGRMVLTIWGRK 230
Y+EQFQRD S+FLKCRA+ELV+GGR+VLT+ GRK
Sbjct: 539 YYEQFQRDLSIFLKCRAKELVEGGRIVLTMVGRK 640
>TC86418 weakly similar to GP|18461100|dbj|BAB84353.
S-adenosyl-L-methionine:salicylic acid calboxyl
methyltransferase-like protein {Cucumis sativus},
partial (39%)
Length = 1306
Score = 246 bits (628), Expect = 7e-66
Identities = 122/230 (53%), Positives = 156/230 (67%), Gaps = 6/230 (2%)
Frame = +2
Query: 11 MKVEQVLHMNGGIGETSYSNNSLLQKKVISLTKEMRDEAIKNLYCKTFPKRLGIADLGCS 70
M VEQVLHMNGG G+TSY+NNS Q+ V+ K + +E+I LYC TFP L +ADLGCS
Sbjct: 89 MAVEQVLHMNGGEGDTSYANNSTFQRMVMLTAKHILEESIMRLYCDTFPNCLKVADLGCS 268
Query: 71 SGPNTLLVISEVIKLVDKLCQEHNHESPEYQVFMNDLQGNDFNNIFRLLDRFTEKLNDEV 130
SGPN LLV S +I +D + Q+ +HESP +Q F+NDL GNDFN F+LL F ++L +E
Sbjct: 269 SGPNALLVASNIINTIDAVSQKLSHESPMFQFFLNDLFGNDFNTTFKLLPDFIKRLQEEK 448
Query: 131 EDGIGGPIFFYGAPGSFYGRIFPTKTMHFIHSSYSLQWLSQVPKGVEN------NKGNIY 184
P FF G PGSFYGR+FP ++HF HSSYSL WLS+ P +++ NKGNIY
Sbjct: 449 GQKF-SPCFFSGTPGSFYGRLFPDNSIHFFHSSYSLHWLSKTPDALQDAAIEPLNKGNIY 625
Query: 185 MATTSPANVLNAYHEQFQRDFSLFLKCRAEELVDGGRMVLTIWGRKSDDK 234
+ SP V Y EQFQ+DFSLFL+ R+ EL+ G MVLT+ GR ++
Sbjct: 626 LTRASPPAVQKTYFEQFQQDFSLFLRSRSSELLPSGAMVLTLIGRDEQNE 775
>AW584129 weakly similar to GP|13235641|em S-adenosyl-L-methionine:salicylic
acid carboxyl methyltransferase {Stephanotis
floribunda}, partial (31%)
Length = 568
Score = 221 bits (562), Expect = 3e-58
Identities = 103/165 (62%), Positives = 130/165 (78%)
Frame = +2
Query: 8 ETRMKVEQVLHMNGGIGETSYSNNSLLQKKVISLTKEMRDEAIKNLYCKTFPKRLGIADL 67
ETRM + QV+HMNGG GETSY+ NS Q +SLTK +RDEAI +LY KT K L IADL
Sbjct: 53 ETRMDLGQVVHMNGGDGETSYAKNSFHQGNAVSLTKPIRDEAITSLYSKTLFKSLAIADL 232
Query: 68 GCSSGPNTLLVISEVIKLVDKLCQEHNHESPEYQVFMNDLQGNDFNNIFRLLDRFTEKLN 127
GCSSGPNTL V+S++I +V+KLCQ+ NH SPEY++F ND+ GNDFNNIF+ LD F EKL
Sbjct: 233 GCSSGPNTLFVVSDIIMVVEKLCQQLNHSSPEYKIFFNDVSGNDFNNIFKSLDNFKEKLQ 412
Query: 128 DEVEDGIGGPIFFYGAPGSFYGRIFPTKTMHFIHSSYSLQWLSQV 172
DE++ + +F+G PGSFY R+FP +++HFIHSS+SLQWLS+V
Sbjct: 413 DEIKTKMSS-CYFFGVPGSFYSRVFPNRSLHFIHSSHSLQWLSKV 544
>TC89843 similar to PIR|E85430|E85430 hypothetical protein AT4g36470
[imported] - Arabidopsis thaliana, partial (54%)
Length = 940
Score = 174 bits (441), Expect(2) = 8e-50
Identities = 87/188 (46%), Positives = 120/188 (63%), Gaps = 6/188 (3%)
Frame = +1
Query: 24 GETSYSNNSLLQKKVISLTKEMRDEAIKNLYCKTFPKRLGIADLGCSSGPNTLLVISEVI 83
G+TSY+ NS LQKK K + E ++ LY +T PK +GIADLGCSSGPNTL +I ++
Sbjct: 1 GKTSYAKNSSLQKKASDKVKHIIIETVEELYIETTPKSIGIADLGCSSGPNTLSIIKDIF 180
Query: 84 KLVDKLCQEHNHESPEYQVFMNDLQGNDFNNIFRLLDRFTEKLNDEVEDGIGGPIFFYGA 143
+ + + H S E++V+ NDL NDFN+IF+ L F + LN + ++G IF G
Sbjct: 181 QTIQVTSHKIMHHSTEFRVYFNDLPTNDFNSIFKALPEFQKLLNQDRKNGFPS-IFMGGY 357
Query: 144 PGSFYGRIFPTKTMHFIHSSYSLQWLSQVPKGVEN------NKGNIYMATTSPANVLNAY 197
PGSFYGR+FP +HF+HSS+ L WLS+VP + + NKG +Y+ SP V AY
Sbjct: 358 PGSFYGRLFPNSYLHFVHSSHCLHWLSRVPPTIYDEQKRSLNKGCVYICDKSPEVVSQAY 537
Query: 198 HEQFQRDF 205
++QFQ DF
Sbjct: 538 YKQFQEDF 561
Score = 40.4 bits (93), Expect(2) = 8e-50
Identities = 19/31 (61%), Positives = 24/31 (77%)
Frame = +3
Query: 203 RDFSLFLKCRAEELVDGGRMVLTIWGRKSDD 233
R FSLFL+ R+EELV GG+MVLT GR+ +
Sbjct: 552 RRFSLFLRSRSEELVVGGKMVLTFLGRRGPE 644
>BI271770 weakly similar to GP|13235641|em S-adenosyl-L-methionine:salicylic
acid carboxyl methyltransferase {Stephanotis
floribunda}, partial (31%)
Length = 676
Score = 141 bits (355), Expect = 3e-34
Identities = 73/162 (45%), Positives = 98/162 (60%), Gaps = 5/162 (3%)
Frame = +1
Query: 76 LLVISEVIKLVDKLCQEHNHESPEYQVFMNDLQGNDFNNIFRLLDRFTEKLNDEVEDGIG 135
LLV+S ++K++DK+ NHE P +Q+++NDL NDFN I +LL F + + E +
Sbjct: 4 LLVVSNIMKVIDKISLSLNHELPAFQIYLNDLYENDFNTILKLLPDFHQSIQQERGEN-H 180
Query: 136 GPIFFYGAPGSFYGRIFPTKTMHFIHSSYSLQWLSQVPKGVENN-----KGNIYMATTSP 190
GP F PGSFYGR+FP + F HSSY + WLSQ PK KGNI + SP
Sbjct: 181 GPCFINATPGSFYGRLFPNNYIDFFHSSYCVHWLSQAPKYSTKKAEPLIKGNICITRMSP 360
Query: 191 ANVLNAYHEQFQRDFSLFLKCRAEELVDGGRMVLTIWGRKSD 232
+V Y EQF RDF FL+ R++EL G MVLT+ GR+ +
Sbjct: 361 PSVYEVYVEQFGRDFKNFLRSRSDELAMHGVMVLTLIGREKN 486
>BQ123896 weakly similar to PIR|A86285|A86 protein F9L1.6 [imported] -
Arabidopsis thaliana, partial (31%)
Length = 537
Score = 120 bits (302), Expect = 5e-28
Identities = 64/161 (39%), Positives = 94/161 (57%), Gaps = 6/161 (3%)
Frame = +1
Query: 60 KRLGIADLGCSSGPNTLLVISEVIKLVDKLCQEHNHESPEYQVFMNDLQGNDFNNIFRLL 119
K++ IADLGCS+GPNT + I +I+ ++ + PE+QVF ND NDFN +F+ L
Sbjct: 97 KQICIADLGCSTGPNTFIAIQCIIEAIELQYKSQGLAIPEFQVFFNDQISNDFNTLFKKL 276
Query: 120 DRFTEKLNDEVEDGIGGPIFFYGAPGSFYGRIFPTKTMHFIHSSYSLQWLSQVPKGVEN- 178
F G PGSFYGR+FP ++++ +HSS SL W+S+VPK + +
Sbjct: 277 PS-------------NRNYFAAGVPGSFYGRLFPKESLNVVHSSASLNWISKVPKEITDR 417
Query: 179 -----NKGNIYMATTSPANVLNAYHEQFQRDFSLFLKCRAE 214
NKG I+ T +P V++AY Q+Q+D +FL RA+
Sbjct: 418 SSAACNKGRIHY-TNAPKEVVDAYANQYQKDMEIFLHARAQ 537
>AL365973 similar to GP|13235641|emb S-adenosyl-L-methionine:salicylic acid
carboxyl methyltransferase {Stephanotis floribunda},
partial (9%)
Length = 437
Score = 97.8 bits (242), Expect = 4e-21
Identities = 49/49 (100%), Positives = 49/49 (100%)
Frame = +3
Query: 1 MSEVGKRETRMKVEQVLHMNGGIGETSYSNNSLLQKKVISLTKEMRDEA 49
MSEVGKRETRMKVEQVLHMNGGIGETSYSNNSLLQKKVISLTKEMRDEA
Sbjct: 21 MSEVGKRETRMKVEQVLHMNGGIGETSYSNNSLLQKKVISLTKEMRDEA 167
>BI271462 similar to GP|6651395|gb|A floral nectary-specific protein
{Brassica rapa subsp. pekinensis}, partial (6%)
Length = 492
Score = 79.0 bits (193), Expect(2) = 4e-17
Identities = 42/88 (47%), Positives = 60/88 (67%), Gaps = 4/88 (4%)
Frame = +2
Query: 24 GETSYSNNSLLQKKVISLTKEMRDEAIKNLYCKT--FP-KRLGIADLGCSSGPNTLLVIS 80
GETSY+ NS +Q+K+ISLT + +AI + C T +P ++GIADLGCSSGPN L VIS
Sbjct: 116 GETSYAMNSFVQRKIISLTNQATKKAIVEILCSTKRWPIMKMGIADLGCSSGPNALRVIS 295
Query: 81 EVIKLVDKLCQEHNHESP-EYQVFMNDL 107
E+++ ++ N +P E ++MNDL
Sbjct: 296 EIVEAINATSSMLNRPAPKELMLYMNDL 379
Score = 26.2 bits (56), Expect(2) = 4e-17
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +3
Query: 110 NDFNNIFRLLDRFTEKLNDE 129
NDFNNIF L F +KL+ +
Sbjct: 387 NDFNNIFASLPSFHKKLSQQ 446
>TC83132 similar to GP|21593254|gb|AAM65203.1
S-adenosyl-L-methionine:salicylic acid carboxyl
methyltransferase-like protein, partial (63%)
Length = 1075
Score = 74.3 bits (181), Expect = 5e-14
Identities = 38/98 (38%), Positives = 56/98 (56%), Gaps = 6/98 (6%)
Frame = +1
Query: 142 GAPGSFYGRIFPTKTMHFIHSSYSLQWLSQVP------KGVENNKGNIYMATTSPANVLN 195
G PGSFY R+FP +++ HS++SL WLS++P K + NKG +++ + + N
Sbjct: 4 GVPGSFYRRLFPARSVDVFHSAFSLHWLSKIPESVLDKKSIAYNKGKVFIHGANESTA-N 180
Query: 196 AYHEQFQRDFSLFLKCRAEELVDGGRMVLTIWGRKSDD 233
AY QF+ D + FL R+ E+ G M L GR S D
Sbjct: 181 AYKRQFKTDLASFLSARSVEMKREGSMFLVCLGRTSVD 294
>TC86419 weakly similar to GP|18461100|dbj|BAB84353.
S-adenosyl-L-methionine:salicylic acid calboxyl
methyltransferase-like protein {Cucumis sativus},
partial (32%)
Length = 879
Score = 64.3 bits (155), Expect = 5e-11
Identities = 34/79 (43%), Positives = 47/79 (59%), Gaps = 6/79 (7%)
Frame = +2
Query: 162 SSYSLQWLSQVPKGVEN------NKGNIYMATTSPANVLNAYHEQFQRDFSLFLKCRAEE 215
+SY+ Q P +++ NKGNIY+ SP V Y EQFQ+DFSLFL+ R+ E
Sbjct: 74 TSYANNSTFQTPDALQDAAIEPLNKGNIYLTRASPPAVQKTYFEQFQQDFSLFLRSRSSE 253
Query: 216 LVDGGRMVLTIWGRKSDDK 234
L+ G MVLT+ GR ++
Sbjct: 254 LLPSGAMVLTLIGRDEQNE 310
Score = 42.7 bits (99), Expect = 2e-04
Identities = 19/25 (76%), Positives = 21/25 (84%)
Frame = +2
Query: 11 MKVEQVLHMNGGIGETSYSNNSLLQ 35
M VEQVLHMNGG G+TSY+NNS Q
Sbjct: 29 MAVEQVLHMNGGEGDTSYANNSTFQ 103
>TC85117 similar to GP|21593254|gb|AAM65203.1
S-adenosyl-L-methionine:salicylic acid carboxyl
methyltransferase-like protein, partial (29%)
Length = 557
Score = 63.5 bits (153), Expect = 9e-11
Identities = 43/134 (32%), Positives = 65/134 (48%), Gaps = 7/134 (5%)
Frame = +1
Query: 27 SYSNNSLLQ----KKVISLTKEMRDEA-IKNLYCKTFPKRLGIADLGCSSGPNTLLVISE 81
+Y+NNS Q K +I +E D+ + K +ADLGCS G NT+ V++
Sbjct: 154 AYANNSQAQAIHAKSMIHFLRETLDKVKLGGGGGGDGDKAFVVADLGCSCGSNTINVVNV 333
Query: 82 VIKLVDKLCQEHNHESPEYQVFMNDLQGNDFNNIFRLLDRFTEKLNDE--VEDGIGGPIF 139
+I + K + PE+ + +DL NDFN +F+LL ++ E + F
Sbjct: 334 IINHIIKRYEALGCNPPEFSAYFSDLPSNDFNTLFQLLPPLANGISMEECLXADNQRSYF 513
Query: 140 FYGAPGSFYGRIFP 153
G PGSFY +FP
Sbjct: 514 VAGVPGSFYRXLFP 555
>BM815618 weakly similar to GP|6651395|gb|A floral nectary-specific protein
{Brassica rapa subsp. pekinensis}, partial (24%)
Length = 664
Score = 62.4 bits (150), Expect = 2e-10
Identities = 30/49 (61%), Positives = 40/49 (81%), Gaps = 1/49 (2%)
Frame = +1
Query: 194 LNAYHEQFQRDFSLFLKCRAEELVDGGRMVLTIWGRKSDDKYS-KECGY 241
+ AY++QF+RDFS+FLKCRAEELV+GG MVLT+ GR+++D K C Y
Sbjct: 1 VKAYYDQFKRDFSVFLKCRAEELVEGGCMVLTMPGRRNEDPCDIKYCCY 147
>TC76387 homologue to SP|Q40468|IF4Z_TOBAC Eukaryotic initiation factor
4A-15 (eIF-4A-15) (eIF4A-15). [Common tobacco]
{Nicotiana tabacum}, partial (56%)
Length = 1562
Score = 27.7 bits (60), Expect = 5.2
Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +2
Query: 144 PGSFYGRIFP-TKTMHFIHSSYSLQWLSQVPKGVENNKGNI 183
PGSF+ + P T ++ F SS WL P+G + GN+
Sbjct: 494 PGSFWETLNPFTNSVTFFRSSTLKLWLDLHPRGHNSMLGNL 616
>TC93600
Length = 703
Score = 27.3 bits (59), Expect = 6.8
Identities = 14/37 (37%), Positives = 21/37 (55%), Gaps = 3/37 (8%)
Frame = +3
Query: 146 SFYGRIFPTKTMHF---IHSSYSLQWLSQVPKGVENN 179
+FY +F + ++F IH SYS+ L PKG+ N
Sbjct: 492 NFYYPLFELELLYFFPSIHISYSIYSLLYFPKGLSKN 602
>TC90734 similar to GP|12322994|gb|AAG51484.1 unknown protein {Arabidopsis
thaliana}, partial (55%)
Length = 675
Score = 27.3 bits (59), Expect = 6.8
Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 5/49 (10%)
Frame = -3
Query: 32 SLLQKKVISLTKEMRDE-----AIKNLYCKTFPKRLGIADLGCSSGPNT 75
S L+ K +K+ DE I N+ +F RLG D+GCS N+
Sbjct: 580 SFLENKSCQSSKKRTDEPDS*WVINNILY*SFISRLGKGDVGCS*SKNS 434
>TC87454 similar to SP|P49730|RIR2_TOBAC Ribonucleoside-diphosphate reductase
small chain (EC 1.17.4.1) (Ribonucleotide reductase),
partial (96%)
Length = 1675
Score = 27.3 bits (59), Expect = 6.8
Identities = 14/41 (34%), Positives = 19/41 (46%)
Frame = -1
Query: 158 HFIHSSYSLQWLSQVPKGVENNKGNIYMATTSPANVLNAYH 198
H HSSYS Q LS P+ A + P+N ++ H
Sbjct: 1042 HHFHSSYS*QMLSDTPQRASQKNWFFPEAKSIPSNQTDSEH 920
>NP212723 NP212723|AJ132893.1|CAB85497.1 H+-ATPase
Length = 798
Score = 26.9 bits (58), Expect = 8.9
Identities = 16/36 (44%), Positives = 22/36 (60%)
Frame = -1
Query: 149 GRIFPTKTMHFIHSSYSLQWLSQVPKGVENNKGNIY 184
GRI K M+F+++S L W+SQ P N G+IY
Sbjct: 231 GRI-ERKKMYFLYTSTCLLWISQHP-----NYGSIY 142
>CA920867 similar to GP|10798819|dbj putative damage-specific DNA binding
protein 2 {Oryza sativa (japonica cultivar-group)},
partial (16%)
Length = 761
Score = 26.9 bits (58), Expect = 8.9
Identities = 16/67 (23%), Positives = 28/67 (40%), Gaps = 10/67 (14%)
Frame = +3
Query: 157 MHFIHSSY----------SLQWLSQVPKGVENNKGNIYMATTSPANVLNAYHEQFQRDFS 206
MH IH Y + ++ PK +GN+YM +T + N + ++ +
Sbjct: 105 MHIIHYYY*YPKREVACILYKIIAPAPKTKRRERGNLYMVST*QFGLENLHAQKTEMHIL 284
Query: 207 LFLKCRA 213
+ CRA
Sbjct: 285 AIILCRA 305
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.137 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,041,751
Number of Sequences: 36976
Number of extensions: 109561
Number of successful extensions: 518
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 504
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 507
length of query: 284
length of database: 9,014,727
effective HSP length: 95
effective length of query: 189
effective length of database: 5,502,007
effective search space: 1039879323
effective search space used: 1039879323
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC137669.1