BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137546.8 + phase: 0 /pseudo
(374 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imp... 162 2e-40
BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgari... 126 1e-29
BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [impo... 123 1e-28
BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-li... 108 4e-24
TC90516 101 4e-22
BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - ... 77 2e-20
BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At... 96 3e-20
AW736247 weakly similar to PIR|T14619|T146 reverse transcriptase... 67 9e-12
TC92645 29 2.6
TC77874 similar to GP|6498433|dbj|BAA87836.1 hypothetical protei... 28 5.7
TC88082 similar to GP|10798790|dbj|BAB16439. replication factor ... 28 5.7
TC88145 similar to PIR|T06092|T06092 hypothetical protein T5J17.... 27 9.8
TC81386 similar to GP|10140689|gb|AAG13524.1 putative non-LTR re... 27 9.8
>TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imported] -
Arabidopsis thaliana, partial (6%)
Length = 951
Score = 162 bits (410), Expect = 2e-40
Identities = 91/155 (58%), Positives = 111/155 (70%), Gaps = 1/155 (0%)
Frame = +2
Query: 207 QFVSEFHRNGKLAKGINTTFIVLISKVDNPQRLNDFRPISLVGSMNKIIAKLLANRLRLV 266
+FVSEFHRN KL KGIN+TFI LI KVDNPQRLNDFRPISLVGS+ KI+ KLLANRLR+V
Sbjct: 5 RFVSEFHRNRKLFKGINSTFIALIPKVDNPQRLNDFRPISLVGSLYKILGKLLANRLRVV 184
Query: 267 IGSVVSETQSAFVKKRQILDGILIANEVVDEAFKLKKDLMLFKVDFEKTYDSVDWGYLGE 326
IGSV+S+ QSAFVK RQIL+ + + + + + + K ++ G +
Sbjct: 185 IGSVISDAQSAFVKNRQILEMVFL*QMRLWMRLRN*RKIFCCLRWILKRLITLSIGLIWI 364
Query: 327 VL-KCMSFPVLWRKWIRECVGTATASVLVNGSPTD 360
+ MSF VLWRKWI+ECV TAT SVLVNGSPT+
Sbjct: 365 LF*VGMSFLVLWRKWIKECVSTATTSVLVNGSPTN 469
>BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgaris}, partial
(69%)
Length = 616
Score = 126 bits (317), Expect = 1e-29
Identities = 70/199 (35%), Positives = 113/199 (56%), Gaps = 1/199 (0%)
Frame = +3
Query: 162 LIKPFSVDEVKEAICDYDSFKSSGPDGVNFGFIKEFWIVLKDDIMQFVSEFHRNGKLAKG 221
L F+ EVK A+ DS K+ G DG N F K W ++ D ++ + +F + G + K
Sbjct: 12 LCSEFTAVEVKNALFSMDSSKAPGIDGYNVHFFKCSWNIIGDSVIDAILDFFKTGFMPKI 191
Query: 222 INTTFIVLISKVDNPQRLNDFRPISLVGSMNKIIAKLLANRLRLVIGSVVSETQSAFVKK 281
IN T++ L+ K N + +FRPI+ + KII+K+L +R++ V+ SVVSE QSAFVK
Sbjct: 192 INCTYVTLLPKEVNVTSVKNFRPIACCSVIYKIISKILTSRMQGVLNSVVSENQSAFVKG 371
Query: 282 RQILDGILIANEVVDE-AFKLKKDLMLFKVDFEKTYDSVDWGYLGEVLKCMSFPVLWRKW 340
R I D I++++E+V + K + K+D K YDS +W ++ ++ + FP + W
Sbjct: 372 RVIFDNIILSHELVKSYSRKGISPRCMVKIDLXKAYDSXEWPFIKHLMLELGFPYKFVNW 551
Query: 341 IRECVGTATASVLVNGSPT 359
+ + TA+ + NG T
Sbjct: 552 VMAXLTTASYTFNXNGDLT 608
>BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [imported] -
Arabidopsis thaliana, partial (7%)
Length = 687
Score = 123 bits (308), Expect = 1e-28
Identities = 80/216 (37%), Positives = 116/216 (53%), Gaps = 8/216 (3%)
Frame = +2
Query: 84 WLREGDMNSKFFHSLMSSRRRFNTICSVLVEDVR-IEGVEPVRNAIFTHFAQHFMSHNVV 142
WL++GD N+KFFHS S RR+ N I + E +G E V + T+F F S N
Sbjct: 5 WLKDGDKNTKFFHSKASQRRKVNEIKKLKDETGNWCKGEENVERLLITYFNNLFTSSN-- 178
Query: 143 RPSVSNLQFQALSVTEG----EGLI---KPFSVDEVKEAICDYDSFKSSGPDGVNFGFIK 195
P+ + V +G E ++ K F+ +EV EAI K+ GPDG+ F +
Sbjct: 179 -PTAIE---ETCEVVKGKLSHEHIVWCEKEFTEEEVLEAINQMHPVKAPGPDGLPALFFQ 346
Query: 196 EFWIVLKDDIMQFVSEFHRNGKLAKGINTTFIVLISKVDNPQRLNDFRPISLVGSMNKII 255
++W ++ ++ Q V + N + +N TFIVLI K NP D+RPISL + KII
Sbjct: 347 KYWHIVGKEVQQMVLQVLNNSMETEELNKTFIVLIPKGKNPNTPKDYRPISLCNVVMKII 526
Query: 256 AKLLANRLRLVIGSVVSETQSAFVKKRQILDGILIA 291
K++ANR++ + V+ QSAFV+ R I D LIA
Sbjct: 527 TKVIANRVKQTLPDVIDVEQSAFVQGRLITDNALIA 634
>BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-like protein
{Arabidopsis thaliana}, partial (18%)
Length = 789
Score = 108 bits (269), Expect = 4e-24
Identities = 56/141 (39%), Positives = 85/141 (59%), Gaps = 3/141 (2%)
Frame = -3
Query: 237 QRLNDFRPISLVGSMNKIIAKLLANRLRLVIGSVVSETQSAFVKKRQILDGILIANEVVD 296
+R++++R I+ + KIIAK+L+ R++ ++ S++S +QSAFV R I D +LI ++++
Sbjct: 784 KRVSEYRTIAPCNTQYKIIAKILSKRMQPLLRSIISPSQSAFVPGRAISDNVLITHKILH 605
Query: 297 ---EAFKLKKDLMLFKVDFEKTYDSVDWGYLGEVLKCMSFPVLWRKWIRECVGTATASVL 353
++ K M K D K YD + W +L EVL + F +W WI ECV T + S L
Sbjct: 604 YLRQSGAKKHVSMAVKTDMTKAYDRIAWNFLREVLTRLGFHGIWISWIMECVSTVSYSFL 425
Query: 354 VNGSPTDEFPLKRGLRQGDPL 374
+NG P RGLRQGDPL
Sbjct: 424 INGGPQGRVLPSRGLRQGDPL 362
>TC90516
Length = 983
Score = 101 bits (252), Expect = 4e-22
Identities = 67/210 (31%), Positives = 110/210 (51%)
Frame = -2
Query: 86 REGDMNSKFFHSLMSSRRRFNTICSVLVEDVRIEGVEPVRNAIFTHFAQHFMSHNVVRPS 145
REG N+ +F + + +R N+I + VE ++ V + AI F HF RP+
Sbjct: 754 REGH-NTTYFLACVKNRGMRNSISAPRVERW-MDDVAKNKQAIVNFFTHHFSDP*TYRPT 581
Query: 146 VSNLQFQALSVTEGEGLIKPFSVDEVKEAICDYDSFKSSGPDGVNFGFIKEFWIVLKDDI 205
+ ++ F +S + L F V +++ + D +S PDG N F +LK DI
Sbjct: 580 MGDIDFFHISNLDNVLLSAQFLVSKMELVVSSLDGNESPRPDGFNLNFFIRLRNMLKADI 401
Query: 206 MQFVSEFHRNGKLAKGINTTFIVLISKVDNPQRLNDFRPISLVGSMNKIIAKLLANRLRL 265
+F+ L K + F+ LISKV+ P L DF +S +GS+ K++AK+LA RL
Sbjct: 400 EIMFEQFYTPANLLKIFS*YFLTLISKVEYPILLGDFSLMSFLGSL*KLMAKVLALRLAH 221
Query: 266 VIGSVVSETQSAFVKKRQILDGILIANEVV 295
++ ++ QS FV+ RQ +DG++ NE++
Sbjct: 220 IMEKIIFVNQSTFVRGRQHVDGVVAINEII 131
>BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - Arabidopsis
thaliana, partial (17%)
Length = 677
Score = 76.6 bits (187), Expect(2) = 2e-20
Identities = 39/87 (44%), Positives = 57/87 (64%)
Frame = +3
Query: 193 FIKEFWIVLKDDIMQFVSEFHRNGKLAKGINTTFIVLISKVDNPQRLNDFRPISLVGSMN 252
F + W ++K D+++ V+ F +GKL +NTT I LI K P R+ + RPISL
Sbjct: 411 FFQHSWHIIKMDLLKMVNSFLASGKLDTRLNTTNICLIPKKKRPTRMTELRPISLCNVGY 590
Query: 253 KIIAKLLANRLRLVIGSVVSETQSAFV 279
KII+K+L RL++ + S++SETQSAFV
Sbjct: 591 KIISKVLCQRLKVCLPSLISETQSAFV 671
Score = 39.7 bits (91), Expect(2) = 2e-20
Identities = 33/118 (27%), Positives = 51/118 (42%), Gaps = 5/118 (4%)
Frame = +2
Query: 77 WQQ-SRNHWLREGDMNSKFFHSLMSSRRRFNTICSVLVED----VRIEGVEPVRNAIFTH 131
WQQ SRN W GD+N KF+H+L R N I + D +GVE V F
Sbjct: 53 WQQKSRNMWHISGDLNKKFYHALTKQRHARNRIVGLYDYDGNWITEEQGVEKVAVDYFED 232
Query: 132 FAQHFMSHNVVRPSVSNLQFQALSVTEGEGLIKPFSVDEVKEAICDYDSFKSSGPDGV 189
Q + +++ + L++ + +EV+ A+ K+ GPDG+
Sbjct: 233 LFQRTTPTGF--DGFLDEITSSITPQMNQRLLRLATEEEVRLALFIMHPEKAPGPDGM 400
>BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (16%)
Length = 794
Score = 95.5 bits (236), Expect = 3e-20
Identities = 62/180 (34%), Positives = 91/180 (50%), Gaps = 3/180 (1%)
Frame = +3
Query: 72 NTSICW-QQSRNHWLREGDMNSKFFHSLMSSRRRFNTICSVLVEDVRIEGVEPVRNAIFT 130
N I W Q+SR +WLR GD N+KFFH++ +RR N I S++ +D + VE +
Sbjct: 120 NEEIFWMQKSRLNWLRSGDRNTKFFHAVTKNRRAQNRILSLIDDDDKEWFVEEDLGRLAD 299
Query: 131 HFAQHFMSHNVVRPSVSNLQFQALSVTEGEG--LIKPFSVDEVKEAICDYDSFKSSGPDG 188
+ S V ++ + VTE + L+ S +EV+EA+ D + K GPDG
Sbjct: 300 SHFKLLYSSEDVGITLEDWNSIPAIVTEEQNAQLMAQISREEVREAVFDINPHKCPGPDG 479
Query: 189 VNFGFIKEFWIVLKDDIMQFVSEFHRNGKLAKGINTTFIVLISKVDNPQRLNDFRPISLV 248
+N F ++FW + DD+ EF R GKL +GIN T I SK + + P V
Sbjct: 480 MNVFFFQQFWDTMGDDLTSMAQEFLRTGKLEEGINKTNIWFGSKKAGGKEASGISPHKFV 659
Score = 54.3 bits (129), Expect = 7e-08
Identities = 28/64 (43%), Positives = 45/64 (69%)
Frame = +1
Query: 229 LISKVDNPQRLNDFRPISLVGSMNKIIAKLLANRLRLVIGSVVSETQSAFVKKRQILDGI 288
L+ K +RL +FRPISL KI++K+L+ RL+ V+ +++ETQ+AF +++ I D I
Sbjct: 601 LVPKKLEAKRLVEFRPISLCNVAYKIVSKVLSKRLKSVLPWIITETQAAFGRRQLISDNI 780
Query: 289 LIAN 292
LIA+
Sbjct: 781 LIAH 792
>AW736247 weakly similar to PIR|T14619|T146 reverse transcriptase - beet
retrotransposon (fragment), partial (4%)
Length = 305
Score = 67.4 bits (163), Expect = 9e-12
Identities = 32/58 (55%), Positives = 42/58 (72%), Gaps = 2/58 (3%)
Frame = +1
Query: 166 FSVDEVKEAICDYDSFKS--SGPDGVNFGFIKEFWIVLKDDIMQFVSEFHRNGKLAKG 221
FS +EV++A+ D DS S GPDGVNF F+KE+W ++K D ++ V EFH NGKL KG
Sbjct: 121 FSEEEVRKAVWDCDSSHS*NPGPDGVNFTFVKEYWELIKVDFLRVVMEFHTNGKLGKG 294
>TC92645
Length = 561
Score = 29.3 bits (64), Expect = 2.6
Identities = 28/85 (32%), Positives = 38/85 (43%), Gaps = 5/85 (5%)
Frame = +3
Query: 162 LIKPFSVDEVKEAICDYDSFKSSGPDGVNFGFIKEFWIVLKDDIMQFVSEFHRNG----- 216
L PFSVDE+ AI K+ G DG+N F ++ D +V + G
Sbjct: 306 LTAPFSVDEIWTAIFQMHHDKAFGLDGLNLAVYYRF*NLVGGD-TNYVGLYSLVG*R*VA 482
Query: 217 KLAKGINTTFIVLISKVDNPQRLND 241
L G N IVL+ K D+P + D
Sbjct: 483 *LYWGTN---IVLVPKCDSPSTMWD 548
>TC77874 similar to GP|6498433|dbj|BAA87836.1 hypothetical protein {Oryza
sativa (japonica cultivar-group)}, partial (42%)
Length = 1747
Score = 28.1 bits (61), Expect = 5.7
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 122 EPVRNAIFTHFAQHFMSH 139
EP+ IF HF +HFM H
Sbjct: 1534 EPLSQLIFLHFMKHFMKH 1481
>TC88082 similar to GP|10798790|dbj|BAB16439. replication factor C 36kDa
subunit {Oryza sativa (japonica cultivar-group)},
partial (88%)
Length = 1354
Score = 28.1 bits (61), Expect = 5.7
Identities = 11/24 (45%), Positives = 16/24 (65%)
Frame = -2
Query: 163 IKPFSVDEVKEAICDYDSFKSSGP 186
IKPFS+D + +++ D DS K P
Sbjct: 696 IKPFSIDNMFQSLSDMDSIKRGKP 625
>TC88145 similar to PIR|T06092|T06092 hypothetical protein T5J17.30 -
Arabidopsis thaliana, partial (61%)
Length = 1329
Score = 27.3 bits (59), Expect = 9.8
Identities = 21/89 (23%), Positives = 36/89 (39%)
Frame = -1
Query: 191 FGFIKEFWIVLKDDIMQFVSEFHRNGKLAKGINTTFIVLISKVDNPQRLNDFRPISLVGS 250
F FI W+ + + F SE LA I ++N R +FRP +GS
Sbjct: 348 FHFIP*AWLAFGERLSFFFSELVAIENLADSIRRLTRAGGGGINNNGRRRNFRPSEEIGS 169
Query: 251 MNKIIAKLLANRLRLVIGSVVSETQSAFV 279
++ + A+ + + V E S ++
Sbjct: 168 VSVCFSHRSASHCWMDVNVYVFEIHSLYL 82
>TC81386 similar to GP|10140689|gb|AAG13524.1 putative non-LTR retroelement
reverse transcriptase {Oryza sativa (japonica
cultivar-group)}, partial (1%)
Length = 798
Score = 27.3 bits (59), Expect = 9.8
Identities = 11/31 (35%), Positives = 19/31 (60%)
Frame = -1
Query: 78 QQSRNHWLREGDMNSKFFHSLMSSRRRFNTI 108
++S+ +W EGD N+ FFH + +R + I
Sbjct: 96 EKSKVNWHCEGDRNTAFFHRVAKIKRTSSLI 4
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.333 0.145 0.461
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,828,655
Number of Sequences: 36976
Number of extensions: 174618
Number of successful extensions: 1519
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1501
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1515
length of query: 374
length of database: 9,014,727
effective HSP length: 98
effective length of query: 276
effective length of database: 5,391,079
effective search space: 1487937804
effective search space used: 1487937804
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.5 bits)
S2: 59 (27.3 bits)
Medicago: description of AC137546.8