BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137077.10 + phase: 0
(247 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77005 similar to SP|P21653|TIP1_TOBAC Tonoplast intrinsic prot... 484 e-137
TC77007 similar to PIR|T10804|T10804 tonoplast intrinsic protein... 382 e-107
TC77711 similar to PIR|T46160|T46160 caffeic acid O-methyltransf... 366 e-102
TC85305 aquaporin 307 3e-84
TC77008 similar to PIR|A84653|A84653 hypothetical protein At2g25... 257 3e-69
TC78780 similar to PIR|JQ1106|JQ1106 tonoplast intrinsic protein... 253 4e-68
TC77651 similar to PIR|JQ1106|JQ1106 tonoplast intrinsic protein... 248 2e-66
AW736472 homologue to SP|P21653|TIP1 Tonoplast intrinsic protein... 226 5e-60
BI266516 similar to PIR|JQ2288|JQ2 SPCP2 protein - soybean, part... 149 1e-36
TC76539 aquaporin [Medicago truncatula] 148 2e-36
TC78388 homologue to GP|8071620|gb|AAF71816.1| putative aquapori... 148 2e-36
TC85263 homologue to GP|5139539|emb|CAB45651.1 putative plasma m... 142 9e-35
TC85260 homologue to GP|3158476|gb|AAC17529.1| aquaporin 2 {Sama... 137 4e-33
TC85259 homologue to PIR|T09260|T09260 aquaporin-like transmembr... 129 8e-31
TC76601 aquaporin-like protein PIP2 [Medicago truncatula] 126 7e-30
TC88670 similar to GP|535780|dbj|BAA05654.1| transmembrane prote... 121 3e-28
TC89494 homologue to GP|13447793|gb|AAK26754.1 plasma membrane i... 120 6e-28
TC86110 multifunctional aquaporin [Medicago truncatula] 114 3e-26
TC77955 similar to GP|5139541|emb|CAB45652.1 nodulin26-like majo... 114 3e-26
TC93480 similar to PIR|B96840|B96840 hypothetical protein F23A5.... 75 7e-23
>TC77005 similar to SP|P21653|TIP1_TOBAC Tonoplast intrinsic protein
root-specific RB7-5A (RT-TIP). [Common tobacco]
{Nicotiana tabacum}, partial (97%)
Length = 1055
Score = 484 bits (1247), Expect = e-137
Identities = 247/247 (100%), Positives = 247/247 (100%)
Frame = +1
Query: 1 MVKIGFGTFDDSFSAASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVA 60
MVKIGFGTFDDSFSAASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVA
Sbjct: 112 MVKIGFGTFDDSFSAASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVA 291
Query: 61 VAHAFALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLN 120
VAHAFALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLN
Sbjct: 292 VAHAFALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLN 471
Query: 121 YVTAKSVPTHGVAAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFVV 180
YVTAKSVPTHGVAAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFVV
Sbjct: 472 YVTAKSVPTHGVAAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFVV 651
Query: 181 GANILAAGPFSGGSMNPARSFGPAVVSGNFADNWIYWVGPLIGGGLAGLIYGDVFIGSYT 240
GANILAAGPFSGGSMNPARSFGPAVVSGNFADNWIYWVGPLIGGGLAGLIYGDVFIGSYT
Sbjct: 652 GANILAAGPFSGGSMNPARSFGPAVVSGNFADNWIYWVGPLIGGGLAGLIYGDVFIGSYT 831
Query: 241 PAPASEF 247
PAPASEF
Sbjct: 832 PAPASEF 852
>TC77007 similar to PIR|T10804|T10804 tonoplast intrinsic protein delta
type - upland cotton, complete
Length = 1041
Score = 382 bits (980), Expect = e-107
Identities = 193/248 (77%), Positives = 212/248 (84%), Gaps = 1/248 (0%)
Frame = +2
Query: 1 MVKIGFGTFDDSFSAASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVA 60
M I FG DDSFS S+KAY++EFI+TL+FVFAGVGSAIAY LTSDAALDPAGL+AVA
Sbjct: 77 MAGIAFGRLDDSFSFGSIKAYIAEFISTLLFVFAGVGSAIAYGKLTSDAALDPAGLLAVA 256
Query: 61 VAHAFALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLN 120
V H FALFV VA+ ANISGGH+NPAVTFGLA+GG ITILTG+FYWIAQLLGSIVA LL
Sbjct: 257 VCHGFALFVAVAVGANISGGHVNPAVTFGLAVGGQITILTGIFYWIAQLLGSIVACFLLQ 436
Query: 121 YVTAK-SVPTHGVAAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFV 179
+VT P H VAA + PI G+V EIIITFGLVYTVYATAADPKKGSIGTIAPIAIGF+
Sbjct: 437 FVTGGLETPIHSVAAEVGPIGGVVTEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFI 616
Query: 180 VGANILAAGPFSGGSMNPARSFGPAVVSGNFADNWIYWVGPLIGGGLAGLIYGDVFIGSY 239
VGANILAAGPFSGGSMNPARSFGPAVVSGNF DNWIYW GPLIGGGLAGLIYG+VF+ +
Sbjct: 617 VGANILAAGPFSGGSMNPARSFGPAVVSGNFHDNWIYWAGPLIGGGLAGLIYGNVFMHTE 796
Query: 240 TPAPASEF 247
+S+F
Sbjct: 797 HAPLSSDF 820
>TC77711 similar to PIR|T46160|T46160 caffeic acid O-methyltransferase-like
protein - Arabidopsis thaliana, partial (86%)
Length = 2361
Score = 366 bits (939), Expect = e-102
Identities = 186/245 (75%), Positives = 210/245 (84%), Gaps = 1/245 (0%)
Frame = +2
Query: 4 IGFGTFDDSFSAASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAH 63
I FG FDDSFS +S++AY++EFI+TLIFVFAGVGSAIAY LTS AALDPAGLVAVAV H
Sbjct: 80 IAFGRFDDSFSVSSIRAYVAEFISTLIFVFAGVGSAIAYAKLTSGAALDPAGLVAVAVCH 259
Query: 64 AFALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYVT 123
FALFV V++ ANISGGH+NPAVTFGLAIGG ITILTG+FYWIAQLLGSIVA LL Y T
Sbjct: 260 GFALFVAVSVGANISGGHVNPAVTFGLAIGGQITILTGIFYWIAQLLGSIVACFLLKYAT 439
Query: 124 -AKSVPTHGVAAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFVVGA 182
++P H VA+G+ G+V EIIITFGLVYTVYATAADPKKGS+GTIAPIAIG +VGA
Sbjct: 440 GGLTIPIHSVASGVGAGEGVVTEIIITFGLVYTVYATAADPKKGSLGTIAPIAIGLIVGA 619
Query: 183 NILAAGPFSGGSMNPARSFGPAVVSGNFADNWIYWVGPLIGGGLAGLIYGDVFIGSYTPA 242
NILAAGPFSGGSMNPARSFGPAV+SG++ +NWIYWVGPLIGGGLAG+IY VF+ S
Sbjct: 620 NILAAGPFSGGSMNPARSFGPAVLSGDYHNNWIYWVGPLIGGGLAGVIYSYVFMPSDHVP 799
Query: 243 PASEF 247
AS+F
Sbjct: 800 LASDF 814
>TC85305 aquaporin
Length = 1221
Score = 307 bits (786), Expect = 3e-84
Identities = 153/235 (65%), Positives = 187/235 (79%)
Frame = +2
Query: 4 IGFGTFDDSFSAASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAH 63
I GT ++ +LKA L+EFI+T IFVFAG GS IAYN LT+D A PAGL++ ++AH
Sbjct: 113 IAVGTPQEATHPDTLKAGLAEFISTFIFVFAGSGSGIAYNKLTNDGAATPAGLISASIAH 292
Query: 64 AFALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYVT 123
AFALFV V++ ANISGGH+NPAVTFG +GGNIT+L G+ Y IAQLLGSIVAS LL +VT
Sbjct: 293 AFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIVYIIAQLLGSIVASALLVFVT 472
Query: 124 AKSVPTHGVAAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFVVGAN 183
A SVP G++ G+ LV EI++TFGLVYTVYATA DPKKG+IG IAPIAIGF+VGAN
Sbjct: 473 ASSVPAFGLSEGVGVGPALVLEIVMTFGLVYTVYATAVDPKKGNIGIIAPIAIGFIVGAN 652
Query: 184 ILAAGPFSGGSMNPARSFGPAVVSGNFADNWIYWVGPLIGGGLAGLIYGDVFIGS 238
IL G F+G SMNPA SFGPAVVS +++++W+YW GPLIGGG+AGL+Y +FI S
Sbjct: 653 ILVGGAFTGASMNPAVSFGPAVVSWSWSNHWVYWAGPLIGGGIAGLVYEVLFINS 817
>TC77008 similar to PIR|A84653|A84653 hypothetical protein At2g25810
[imported] - Arabidopsis thaliana, partial (97%)
Length = 967
Score = 257 bits (656), Expect = 3e-69
Identities = 130/237 (54%), Positives = 172/237 (71%), Gaps = 1/237 (0%)
Frame = +2
Query: 1 MVKIGFGTFDDSFSAASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVA 60
M KI GT ++ ++A + EFIAT +FVFAGVGSA+ + L+ DA + GL VA
Sbjct: 23 MAKIALGTTREATQPDCIQALIVEFIATFLFVFAGVGSAMTADKLSGDALV---GLFFVA 193
Query: 61 VAHAFALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLN 120
+AHA + V ++ AA+ISGGHLNPAVT GL +GG+ITI+ + YWI QL+ S A LL+
Sbjct: 194 IAHALVVAVMIS-AAHISGGHLNPAVTLGLLVGGHITIVRSILYWIDQLIASAAACYLLH 370
Query: 121 YVTAK-SVPTHGVAAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFV 179
Y++ + P H +A+G+ G+V+EI++TF L++TVYAT DPKKG++ + P +GFV
Sbjct: 371 YLSGGLTTPAHTLASGVGYTQGVVWEIVLTFSLLFTVYATMVDPKKGALAGLGPTLVGFV 550
Query: 180 VGANILAAGPFSGGSMNPARSFGPAVVSGNFADNWIYWVGPLIGGGLAGLIYGDVFI 236
VGANILA G FS SMNPARSFGPA+VSGN+ D+W+YWVGPLIGGGLAG IY + FI
Sbjct: 551 VGANILAGGAFSAASMNPARSFGPALVSGNWTDHWVYWVGPLIGGGLAGFIYENFFI 721
>TC78780 similar to PIR|JQ1106|JQ1106 tonoplast intrinsic protein alpha -
kidney bean, partial (95%)
Length = 999
Score = 253 bits (647), Expect = 4e-68
Identities = 128/240 (53%), Positives = 169/240 (70%), Gaps = 1/240 (0%)
Frame = +1
Query: 3 KIGFGTFDDSFSAASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVA 62
+ FG D+++ S++A ++EF +T IFVFAG GS +A + D+A L+AVA+A
Sbjct: 31 RYAFGRVDEAYHPDSIRATIAEFASTFIFVFAGEGSGLALVKIYQDSAFSAGELLAVALA 210
Query: 63 HAFALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYV 122
HAFALF V+ + ++SGGH+NPAVTFG IGG I++L ++YWIAQLLG+IVA+LLL V
Sbjct: 211 HAFALFAAVSSSMHVSGGHVNPAVTFGALIGGRISVLRAVYYWIAQLLGAIVAALLLRLV 390
Query: 123 TAKSVPT-HGVAAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFVVG 181
T P +A G+ GL+ EII+TFGL+YTVYATA DPK+GSIG IAP+AIG +VG
Sbjct: 391 TNNMRPGGFHLARGIGVGHGLILEIIMTFGLMYTVYATAIDPKRGSIGAIAPLAIGLIVG 570
Query: 182 ANILAAGPFSGGSMNPARSFGPAVVSGNFADNWIYWVGPLIGGGLAGLIYGDVFIGSYTP 241
ANIL GPF G MNPA +FGP++V + +WI+WVGP IG LA LIY + I + P
Sbjct: 571 ANILVGGPFDGACMNPALAFGPSLVGWRWHYHWIFWVGPFIGAALAALIYEYLVIPTEPP 750
>TC77651 similar to PIR|JQ1106|JQ1106 tonoplast intrinsic protein alpha -
kidney bean, partial (98%)
Length = 1154
Score = 248 bits (632), Expect = 2e-66
Identities = 121/235 (51%), Positives = 163/235 (68%), Gaps = 1/235 (0%)
Frame = +3
Query: 3 KIGFGTFDDSFSAASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVA 62
+ FG D++ S++A ++EF +T IFVFAG GSA+A + DA L+ +A+A
Sbjct: 138 RYAFGRMDEANHPDSIRATVAEFFSTCIFVFAGEGSALALRKIYKDAGASSGELLVLAIA 317
Query: 63 HAFALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYV 122
HAF+LF ++ + ++SGGH+NPAVTFG +GG I++L L+YW+AQLLGS VA+LLL V
Sbjct: 318 HAFSLFAAISSSMHVSGGHINPAVTFGALLGGRISVLRALYYWVAQLLGSFVAALLLRLV 497
Query: 123 TAKSVP-THGVAAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFVVG 181
T P VA G+ LV EI +TFGL+Y VYATA DPK+G+IGTIAP+AI VVG
Sbjct: 498 TNNMRPQAFNVAFGVGAGNALVLEIAMTFGLMYVVYATAIDPKRGTIGTIAPLAIALVVG 677
Query: 182 ANILAAGPFSGGSMNPARSFGPAVVSGNFADNWIYWVGPLIGGGLAGLIYGDVFI 236
ANILA GPF G MNPAR+FGPA+V + +WI+WVGP +G +A L+Y + +
Sbjct: 678 ANILAGGPFDGACMNPARAFGPALVGWRWHYHWIFWVGPFLGAAIAALLYEYIMV 842
Score = 40.8 bits (94), Expect = 5e-04
Identities = 29/84 (34%), Positives = 39/84 (45%), Gaps = 3/84 (3%)
Frame = +3
Query: 50 ALDPA-GLVAVAVAHAFALFVGVAIAAN--ISGGHLNPAVTFGLAIGGNITILTGLFYWI 106
A+DP G + A AL VG I A G +NPA FG A+ G +F W+
Sbjct: 612 AIDPKRGTIGTIAPLAIALVVGANILAGGPFDGACMNPARAFGPALVGWRWHYHWIF-WV 788
Query: 107 AQLLGSIVASLLLNYVTAKSVPTH 130
LG+ +A+LL Y+ P H
Sbjct: 789 GPFLGAAIAALLYEYIMVPVEPPH 860
>AW736472 homologue to SP|P21653|TIP1 Tonoplast intrinsic protein
root-specific RB7-5A (RT-TIP). [Common tobacco]
{Nicotiana tabacum}, partial (43%)
Length = 691
Score = 226 bits (577), Expect = 5e-60
Identities = 115/131 (87%), Positives = 124/131 (93%)
Frame = -2
Query: 26 IATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHAFALFVGVAIAANISGGHLNPA 85
+ATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHAFALFVGVAIAANISGGHLNPA
Sbjct: 597 VATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHAFALFVGVAIAANISGGHLNPA 418
Query: 86 VTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYVTAKSVPTHGVAAGLNPIAGLVFE 145
VTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYVTAKSVPTHGVAAGLNPIA + +
Sbjct: 417 VTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYVTAKSVPTHGVAAGLNPIARIRYS 238
Query: 146 IIITFGLVYTV 156
+++ L+ ++
Sbjct: 237 VLVPIWLLLSI 205
Score = 38.9 bits (89), Expect = 0.002
Identities = 29/101 (28%), Positives = 44/101 (42%), Gaps = 3/101 (2%)
Frame = -2
Query: 133 AAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGSIGTIA-PIAIGFVVGANILAAGPFS 191
A G +A L+F + G +D G +A +A F + + A S
Sbjct: 615 ATGARCVATLIF-VFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHAFALFVGVAIAANIS 439
Query: 192 GGSMNPARSFGPAVVSGNFA--DNWIYWVGPLIGGGLAGLI 230
GG +NPA +FG A + GN YW+ L+G +A L+
Sbjct: 438 GGHLNPAVTFGLA-IGGNITILTGLFYWIAQLLGSIVASLL 319
>BI266516 similar to PIR|JQ2288|JQ2 SPCP2 protein - soybean, partial (56%)
Length = 549
Score = 149 bits (375), Expect(2) = 1e-36
Identities = 75/121 (61%), Positives = 93/121 (75%)
Frame = +2
Query: 3 KIGFGTFDDSFSAASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVA 62
+I G + A +LKA L+EFI+ LIFVFAG GS +AYN LT++ A PAGLVA +++
Sbjct: 95 RIAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVAASLS 274
Query: 63 HAFALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYV 122
HAFALFV V++ ANISGGH+NPAVTFG IGG+IT++ GL YWIAQLLGS+VA LLL
Sbjct: 275 HAFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLKIA 454
Query: 123 T 123
T
Sbjct: 455 T 457
Score = 34.7 bits (78), Expect = 0.035
Identities = 25/75 (33%), Positives = 37/75 (49%), Gaps = 2/75 (2%)
Frame = +2
Query: 158 ATAADPKKGSIGTIAPIAIGFVVGANILAAGPFSGGSMNPARSFGPAVVSGNFA--DNWI 215
AT A S+ + + VGANI SGG +NPA +FG A + G+ +
Sbjct: 239 ATPAGLVAASLSHAFALFVAVSVGANI------SGGHVNPAVTFG-AFIGGHITLIRGLL 397
Query: 216 YWVGPLIGGGLAGLI 230
YW+ L+G +A L+
Sbjct: 398 YWIAQLLGSVVACLL 442
Score = 21.6 bits (44), Expect(2) = 1e-36
Identities = 9/11 (81%), Positives = 10/11 (90%)
Frame = +3
Query: 142 LVFEIIITFGL 152
LVFEI +TFGL
Sbjct: 516 LVFEIXMTFGL 548
>TC76539 aquaporin [Medicago truncatula]
Length = 1423
Score = 148 bits (373), Expect = 2e-36
Identities = 92/244 (37%), Positives = 136/244 (55%), Gaps = 17/244 (6%)
Frame = +1
Query: 19 KAYLSEFIATLIFVFAGVGSAIAYN-----DLTSDAALDPAGLVAVAVAHAFALFVGVAI 73
+A ++EF+ATL+F++ V + I Y+ + + D G++ +A A +F+ V
Sbjct: 271 RAVIAEFVATLLFLYITVLTIIGYSRQSDTTIKGNTECDGVGVLGIAWAFGGMIFILVYC 450
Query: 74 AANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLL-----LNYVTAKSVP 128
A ISGGH+NPAVTFGL +G ++++ Y +AQ LG+I + L +Y
Sbjct: 451 TAGISGGHINPAVTFGLFVGRKVSLIRAFLYIVAQCLGAICGAGLAKGFQTSYYNRYKGG 630
Query: 129 THGVAAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGS----IGTIAPIAIGFVVGANI 184
+ V+ G + L EII TF LVYTV+ +A DPK+ + + +AP+ IGF V
Sbjct: 631 VNTVSDGYSKGTALGAEIIGTFVLVYTVF-SATDPKRSARDSHVPVLAPLPIGFAVFMVH 807
Query: 185 LAAGPFSGGSMNPARSFGPAVVSGN---FADNWIYWVGPLIGGGLAGLIYGDVFIGSYTP 241
LA P +G +NPARSFGPAV+ N + D WIYWVGP IG +A + + + GS
Sbjct: 808 LATIPVTGTGINPARSFGPAVIFNNEKAWDDQWIYWVGPFIGAAVAAIYHQYILRGSAIK 987
Query: 242 APAS 245
A S
Sbjct: 988 ALGS 999
>TC78388 homologue to GP|8071620|gb|AAF71816.1| putative aquaporin PIP2-1
{Vitis berlandieri x Vitis rupestris}, partial (91%)
Length = 1120
Score = 148 bits (373), Expect = 2e-36
Identities = 90/229 (39%), Positives = 131/229 (56%), Gaps = 16/229 (6%)
Frame = +2
Query: 19 KAYLSEFIATLIFVFAGVGSAIAYNDLTSDAA----LDPAGLVAVAVAHAFALFVGVAIA 74
+A ++EF+ATL+F++ V + I YN T A D G++ +A A +FV V
Sbjct: 146 RALIAEFVATLLFLYVTVLTVIGYNAQTDPAHNGTNCDGVGILGIAWAFGGMIFVLVYCT 325
Query: 75 ANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSI-----VASLLLNYVTAKSVPT 129
A ISGGH+NPAVTFGL + ++++ + Y +AQ LG+I V + Y
Sbjct: 326 AGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGLVKAFQKGYYNRYKGGA 505
Query: 130 HGVAAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGS----IGTIAPIAIGFVVGANIL 185
+ ++AG + GL EII TF LVYTV+ +A DPK+ + + +AP+ IGF V L
Sbjct: 506 NMLSAGYSKGTGLGAEIIGTFVLVYTVF-SATDPKRNARDSHVPVLAPLPIGFAVFMVHL 682
Query: 186 AAGPFSGGSMNPARSFGPAVVSGN---FADNWIYWVGPLIGGGLAGLIY 231
A P +G +NPARSFG AV+ N + D WI+WVGP IG +A + +
Sbjct: 683 ATIPITGTGINPARSFGAAVIYNNEKAWDDQWIFWVGPFIGAAIAAIYH 829
>TC85263 homologue to GP|5139539|emb|CAB45651.1 putative plasma membrane
intrinsic protein {Pisum sativum}, complete
Length = 1359
Score = 142 bits (359), Expect = 9e-35
Identities = 91/243 (37%), Positives = 133/243 (54%), Gaps = 16/243 (6%)
Frame = +3
Query: 19 KAYLSEFIATLIFVFAGVGSAIAYNDLTSDAA----LDPAGLVAVAVAHAFALFVGVAIA 74
+A ++EF+ATL+F++ V + I Y+ + A D G++ +A A +F+ V
Sbjct: 162 RALIAEFVATLLFLYVTVLTIIGYSHQSDPKAGGTDCDGVGILGIAWAFGGMIFILVYCT 341
Query: 75 ANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYVTAKSVPTHGVAA 134
A ISGGH+NPAVTFGL +G ++++ +FY AQ G+I + L +G A
Sbjct: 342 AGISGGHINPAVTFGLFVGRKVSLIRAVFYMAAQSAGAICGTGLAKGFQKAYFDRYGGGA 521
Query: 135 -----GLNPIAGLVFEIIITFGLVYTVYATAADPKKGS----IGTIAPIAIGFVVGANIL 185
G N L EII TF LVYTV+ +A DPK+ + + +AP+ IGF V L
Sbjct: 522 NFVHDGYNKGTALGAEIIGTFVLVYTVF-SATDPKRNARDSHVPVLAPLPIGFAVFMVHL 698
Query: 186 AAGPFSGGSMNPARSFGPAVVSGN---FADNWIYWVGPLIGGGLAGLIYGDVFIGSYTPA 242
A P +G +NPARSFG AV+ + + D WI+WVGP IG +A + + + GS A
Sbjct: 699 ATIPVTGTGINPARSFGSAVIYNDGKIWDDQWIFWVGPFIGAAVAAIYHQYILRGSAIKA 878
Query: 243 PAS 245
S
Sbjct: 879 LGS 887
>TC85260 homologue to GP|3158476|gb|AAC17529.1| aquaporin 2 {Samanea saman},
complete
Length = 1282
Score = 137 bits (345), Expect = 4e-33
Identities = 87/229 (37%), Positives = 128/229 (54%), Gaps = 16/229 (6%)
Frame = +3
Query: 19 KAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPA----GLVAVAVAHAFALFVGVAIA 74
+A ++EFIATL+F++ V + I Y+ T A A G++ +A A +FV V
Sbjct: 201 RALIAEFIATLLFLYVTVLTVIGYSIQTDVKAGGDACGGVGILGIAWAFGGMIFVLVYCT 380
Query: 75 ANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYVTAKSVPTHGVAA 134
A ISGGH+NPAVTFGL + ++++ + Y +AQ LG+I L+ + +G A
Sbjct: 381 AGISGGHINPAVTFGLFLARKVSLIRAIMYMVAQCLGAIAGVGLVKAFQSAYFDRYGGGA 560
Query: 135 -----GLNPIAGLVFEIIITFGLVYTVYATAADPKKGS----IGTIAPIAIGFVVGANIL 185
G + GL EI+ TF LVYTV+ +A DPK+ + + +AP+ IGF V L
Sbjct: 561 NFLHDGYSTGVGLGAEIVGTFVLVYTVF-SATDPKRSARDSHVPVLAPLPIGFAVFMVHL 737
Query: 186 AAGPFSGGSMNPARSFGPAVVSGN---FADNWIYWVGPLIGGGLAGLIY 231
A P +G +NPARS G AV+ + D+WI+WVGP IG +A +
Sbjct: 738 ATIPVTGTGINPARSLGSAVIYNKDKPWDDHWIFWVGPFIGAAIAAFYH 884
>TC85259 homologue to PIR|T09260|T09260 aquaporin-like transmembrane channel
protein - alfalfa, complete
Length = 1502
Score = 129 bits (325), Expect = 8e-31
Identities = 80/226 (35%), Positives = 126/226 (55%), Gaps = 13/226 (5%)
Frame = +3
Query: 19 KAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHAFALFVGVAIAANIS 78
+A ++EF+AT +F++ + + + N S++ G+ +A + +F V A IS
Sbjct: 243 RAGIAEFVATFLFLYITILTVMGVN--RSESKCKTVGIQGIAWSFGGMIFALVYCTAGIS 416
Query: 79 GGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLL------NYVTAKSVPTHGV 132
GGH+NPAVTFGL + +++ +FY + Q LG+I + ++ + T K+ + V
Sbjct: 417 GGHINPAVTFGLFLARKLSLTRAVFYMVMQTLGAICGAGVVKGFEGKTFYTNKNGGANFV 596
Query: 133 AAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGS----IGTIAPIAIGFVVGANILAAG 188
A G GL EI+ TF LVYTV+ +A D K+ + + +AP+ IGF V LA
Sbjct: 597 APGYTKGDGLGAEIVGTFVLVYTVF-SATDAKRSARDSHVPILAPLPIGFAVFLVHLATI 773
Query: 189 PFSGGSMNPARSFGPAVVSG---NFADNWIYWVGPLIGGGLAGLIY 231
P +G +NPARS G A++ + D+WI+WVGP IG LA L +
Sbjct: 774 PITGTGINPARSLGAAIIFNKDLGWDDHWIFWVGPFIGAALAALYH 911
>TC76601 aquaporin-like protein PIP2 [Medicago truncatula]
Length = 1497
Score = 126 bits (317), Expect = 7e-30
Identities = 81/226 (35%), Positives = 124/226 (54%), Gaps = 13/226 (5%)
Frame = +2
Query: 19 KAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHAFALFVGVAIAANIS 78
+A ++EF+AT +F++ V + + + +D+ G+ +A A +F V A IS
Sbjct: 470 RAGIAEFVATFLFLYITVLTVMGV--VRADSKCKTVGIQGIAWAFGGMIFALVYCTAGIS 643
Query: 79 GGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYVTAKS------VPTHGV 132
GGH+NPAVTFGL + +++ LFY + Q LG+I + ++ K+ + V
Sbjct: 644 GGHINPAVTFGLFLARKLSLTRALFYMVMQCLGAICGAGVVKGFEGKTRFGDLKGGANFV 823
Query: 133 AAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGS----IGTIAPIAIGFVVGANILAAG 188
A G GL EI+ TF LVYTV+ +A D K+ + + +AP+ IGF V LA
Sbjct: 824 APGYTKGDGLGAEIVGTFILVYTVF-SATDAKRSARDSHVPILAPLPIGFAVFLVHLATI 1000
Query: 189 PFSGGSMNPARSFGPAVVSG---NFADNWIYWVGPLIGGGLAGLIY 231
P +G +NPARS G A++ + D+WI+WVGP IG LA L +
Sbjct: 1001PITGTGINPARSLGAAIIFNKDLGWDDHWIFWVGPFIGAALAALYH 1138
>TC88670 similar to GP|535780|dbj|BAA05654.1| transmembrane protein
{Arabidopsis thaliana}, complete
Length = 1106
Score = 121 bits (303), Expect = 3e-28
Identities = 80/225 (35%), Positives = 121/225 (53%), Gaps = 12/225 (5%)
Frame = +1
Query: 19 KAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHAFALFVGVAIAANIS 78
+A ++EF+AT +F++ V + + N ++ + G+ +A A +F V A IS
Sbjct: 208 RAGIAEFVATFLFLYITVLTVMGVNKSSNKCS--SVGVQGIAWAFGGMIFALVYCTAGIS 381
Query: 79 GGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYVTAKSVPTHGVAA---- 134
GGH+NPAVTFGL + +++ LFY I Q LG+I + ++ G A
Sbjct: 382 GGHINPAVTFGLFLARKLSLTRTLFYIIMQCLGAICGAAVVKGFQPHQYERLGGGANTLN 561
Query: 135 -GLNPIAGLVFEIIITFGLVYTVYATAADPKKGS----IGTIAPIAIGFVVGANILAAGP 189
G + GL EI+ TF LVYTV+ +A D K+ + + +AP+ IGF V LA P
Sbjct: 562 KGYSIGDGLGAEIVGTFVLVYTVF-SATDAKRNARDSHVPILAPLPIGFAVFLVHLATIP 738
Query: 190 FSGGSMNPARSFGPAVVSGN---FADNWIYWVGPLIGGGLAGLIY 231
+G +NPARS G A++ + D+WI+WVGP G LA L +
Sbjct: 739 ITGTGINPARSLGAALIYNKDQAWDDHWIFWVGPFTGAALAALYH 873
>TC89494 homologue to GP|13447793|gb|AAK26754.1 plasma membrane integral
protein ZmPIP1-3 {Zea mays}, partial (93%)
Length = 1132
Score = 120 bits (300), Expect = 6e-28
Identities = 82/226 (36%), Positives = 127/226 (55%), Gaps = 13/226 (5%)
Frame = +1
Query: 19 KAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHAFALFVGVAIAANIS 78
+A ++EF+AT +F++ + + + N TS + G+ +A + +F V A IS
Sbjct: 196 RAGIAEFVATFLFLYITILTVMGVNRSTSKCS--SVGIQGIAWSFGGMIFALVYCTAGIS 369
Query: 79 GGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYV--TAKSVPTHGVAAGL 136
GGH+NPAVTFGL + +++ +FY Q LG+I + ++ A+ G A +
Sbjct: 370 GGHINPAVTFGLFLARKLSLTRAIFYIGMQCLGAICGAGVVKGFEGNARYELFKGGANFV 549
Query: 137 NP----IAGLVFEIIITFGLVYTVYATAADPKKGS----IGTIAPIAIGFVVGANILAAG 188
NP GL EI+ TF LVYTV+ +A D K+ + + +AP+ IGF V LA
Sbjct: 550 NPGYTKGDGLGAEIVGTFVLVYTVF-SATDAKRNARDSHVPILAPLPIGFAVFLVHLATI 726
Query: 189 PFSGGSMNPARSFGPAVV-SGNFA--DNWIYWVGPLIGGGLAGLIY 231
P +G +NPARS G A++ + +FA D+WI+WVGP IG LA + +
Sbjct: 727 PITGTGINPARSLGAAIIFNRDFAWDDHWIFWVGPFIGAALAAVYH 864
>TC86110 multifunctional aquaporin [Medicago truncatula]
Length = 1411
Score = 114 bits (285), Expect = 3e-26
Identities = 80/246 (32%), Positives = 116/246 (46%), Gaps = 4/246 (1%)
Frame = +3
Query: 5 GFGTFDDSFSAASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHA 64
G G+F S L+ ++E + T +FAG S + D + L +A+
Sbjct: 150 GSGSF---VSVPFLQKLIAEMVGTYFLIFAGCASIVVNKDNDNVVTLP-----GIAIVWG 305
Query: 65 FALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYVTA 124
L V + +ISG H NPAVT A +L Y AQLLG+ +AS L + +
Sbjct: 306 LTLLVLIYSLGHISGAHFNPAVTIAFATTRRFPLLQVPAYISAQLLGATLASGTLKLIFS 485
Query: 125 KSVPTHGVAAGLNP----IAGLVFEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFVV 180
+ H +G P + V E I TF L++T+ A D + +IG +A IAIG +
Sbjct: 486 GA---HDHFSGTLPSGSNLQAFVLEFITTFYLMFTISGVATDTR--AIGELAGIAIGSTL 650
Query: 181 GANILAAGPFSGGSMNPARSFGPAVVSGNFADNWIYWVGPLIGGGLAGLIYGDVFIGSYT 240
N++ AGP +G SMNP R+ GPA V + WIY + P++G +Y V YT
Sbjct: 651 LLNVMIAGPVTGASMNPVRTLGPAFVHNEYRGIWIYLLSPILGAIAGAWVYNTV---RYT 821
Query: 241 PAPASE 246
P E
Sbjct: 822 NKPLRE 839
>TC77955 similar to GP|5139541|emb|CAB45652.1 nodulin26-like major intrinsic
protein {Pisum sativum}, partial (97%)
Length = 1272
Score = 114 bits (285), Expect = 3e-26
Identities = 76/234 (32%), Positives = 113/234 (47%), Gaps = 1/234 (0%)
Frame = +1
Query: 14 SAASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHAFALFVGVAI 73
+A+ L+ ++E I T +FAG S + + + L +++ A+ V V
Sbjct: 241 TASFLQKLVAEVIGTYFLIFAGCASVLVNKNNENVVTLP-----GISIVWGLAVMVLVYS 405
Query: 74 AANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYV-TAKSVPTHGV 132
+ISG H NPAVT A + Y AQ+ GS +AS L + T K G
Sbjct: 406 LGHISGAHFNPAVTIAFASTKRFPLKQVPAYVAAQVFGSTLASGTLRLIFTGKHNQFVGT 585
Query: 133 AAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFVVGANILAAGPFSG 192
+ + V E IITF L++ + A D + +IG +A IA+G V N++ AGP +G
Sbjct: 586 LPAGSDLQAFVIEFIITFYLMFIISGVATDNR--AIGELAGIAVGSTVLLNVMFAGPITG 759
Query: 193 GSMNPARSFGPAVVSGNFADNWIYWVGPLIGGGLAGLIYGDVFIGSYTPAPASE 246
SMNPARS GPA++ + WIY V P++G +Y + YT P E
Sbjct: 760 ASMNPARSIGPALLHSEYRGIWIYLVSPILGAVAGAWVYNVI---RYTDKPVRE 912
>TC93480 similar to PIR|B96840|B96840 hypothetical protein F23A5.11
[imported] - Arabidopsis thaliana, partial (78%)
Length = 902
Score = 74.7 bits (182), Expect(2) = 7e-23
Identities = 34/79 (43%), Positives = 52/79 (65%)
Frame = +1
Query: 145 EIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFVVGANILAAGPFSGGSMNPARSFGPA 204
+ II+F L++ V A A D + ++G +A IA+G V NIL AGP +G SMNP R+ GPA
Sbjct: 664 KFIISFNLMFVVTAVATDTR--AVGELAGIAVGATVMLNILIAGPITGASMNPVRTLGPA 837
Query: 205 VVSGNFADNWIYWVGPLIG 223
+ + N+ W+Y + P++G
Sbjct: 838 IAANNYKAIWVYLLAPILG 894
Score = 49.3 bits (116), Expect(2) = 7e-23
Identities = 36/100 (36%), Positives = 49/100 (49%)
Frame = +3
Query: 23 SEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHAFALFVGVAIAANISGGHL 82
+EFI TLI +FAG +AI N T + L+ A + A+ + + +ISG HL
Sbjct: 312 AEFIGTLILMFAGAATAIV-NQKTQGSET----LIGCATSTGLAVMIIILSTGHISGAHL 476
Query: 83 NPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYV 122
NPAVT A + Y AQ+L SI A+ L V
Sbjct: 477 NPAVTISFAALKHFPWKHVPMYIGAQILASICAAFSLKAV 596
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.141 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,390,638
Number of Sequences: 36976
Number of extensions: 80047
Number of successful extensions: 518
Number of sequences better than 10.0: 87
Number of HSP's better than 10.0 without gapping: 436
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 448
length of query: 247
length of database: 9,014,727
effective HSP length: 94
effective length of query: 153
effective length of database: 5,538,983
effective search space: 847464399
effective search space used: 847464399
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC137077.10