BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136953.11 + phase: 0 /pseudo
(615 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW980456 64 1e-10
BG585866 62 5e-10
TC83226 weakly similar to PIR|G86419|G86419 probable reverse tra... 60 2e-09
BG587113 weakly similar to PIR|A84888|A8 hypothetical protein At... 55 6e-08
BG584442 53 4e-07
TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non... 52 9e-07
BG586862 47 3e-05
CA919100 homologue to PIR|G90291|G902 endoglucanase precursor [i... 44 1e-04
BG456581 41 0.001
AW686588 41 0.002
BG585499 39 0.004
TC81772 39 0.006
BF520135 38 0.010
TC82520 37 0.017
TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarot... 36 0.038
BE941032 35 0.085
AL382240 35 0.11
TC79628 similar to GP|17104799|gb|AAL34288.1 putative 2-nitropro... 33 0.32
BE999296 32 0.94
BQ149324 29 6.1
>AW980456
Length = 779
Score = 64.3 bits (155), Expect = 1e-10
Identities = 33/65 (50%), Positives = 40/65 (60%), Gaps = 2/65 (3%)
Frame = -2
Query: 262 KFERDGAYSVKSAYTNILNH--DVAVVHHRVPDNWTCIWSLKFPSKVKIFLWRACRNCLP 319
K E+ G Y VKSAY + D + +H P NW+ IW LK P KV+ +WR CR CLP
Sbjct: 193 KDEKHGKYYVKSAYRFCVEELFDSSYLHR--PGNWSGIWKLKVPPKVQNLVWRMCRGCLP 20
Query: 320 TRIRL 324
TRIRL
Sbjct: 19 TRIRL 5
>BG585866
Length = 828
Score = 62.4 bits (150), Expect = 5e-10
Identities = 33/98 (33%), Positives = 48/98 (48%)
Frame = +3
Query: 266 DGAYSVKSAYTNILNHDVAVVHHRVPDNWTCIWSLKFPSKVKIFLWRACRNCLPTRIRLQ 325
+G Y+ KS Y+ IL+ V ++ +W+ IW LK P K K FLW AC N +PT L
Sbjct: 375 NGVYTAKSGYSWILSQTETVNYNN--SSWSWIWRLKIPEKYKFFLWLACHNAVPTLSLLN 548
Query: 326 SKGVQCTDKCAVRDDFAEDSTHLFFMCNKSMICWQRSG 363
+ + + C+ + E H C S I W + G
Sbjct: 549 HRNMVNSAICSRCGEHEESFFHCVRDCRFSKIIWHKIG 662
>TC83226 weakly similar to PIR|G86419|G86419 probable reverse transcriptase
100033-105622 [imported] - Arabidopsis thaliana, partial
(2%)
Length = 885
Score = 60.5 bits (145), Expect = 2e-09
Identities = 35/105 (33%), Positives = 46/105 (43%), Gaps = 5/105 (4%)
Frame = +3
Query: 267 GAYSVKSAYTNILNHDVAVVHHRVPDN-----WTCIWSLKFPSKVKIFLWRACRNCLPTR 321
G YSVKS Y + +++ + W IWSL + K+ LWR + LP R
Sbjct: 24 GIYSVKSGYNTLRTWQTQQINNTSTSSDETLIWKKIWSLHTIPRHKVLLWRILNDSLPVR 203
Query: 322 IRLQSKGVQCTDKCAVRDDFAEDSTHLFFMCNKSMICWQRSGLWI 366
L+ +G+QC C E THLF C S W S L I
Sbjct: 204 SSLRKRGIQCYPLCPRCHSKTETITHLFMSCPLSKRVWFGSNLCI 338
>BG587113 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (10%)
Length = 767
Score = 55.5 bits (132), Expect = 6e-08
Identities = 35/121 (28%), Positives = 53/121 (42%), Gaps = 6/121 (4%)
Frame = -3
Query: 262 KFERDGAYSVKSAY---TNIL---NHDVAVVHHRVPDNWTCIWSLKFPSKVKIFLWRACR 315
++ + G YSVKS Y TNI+ N V + D + +W KV+ FLWR
Sbjct: 411 EYSKSGHYSVKSGYYVQTNIIAAANQRGTVDQPSLDDLYQRVWKYNTSPKVRHFLWRCIS 232
Query: 316 NCLPTRIRLQSKGVQCTDKCAVRDDFAEDSTHLFFMCNKSMICWQRSGLWILLMAIFYID 375
N LPT ++S+ + C+ +E H+ F C + + W S + I Y
Sbjct: 231 NSLPTAANMRSRHISKDGSCSRCGMESETVNHILFQCPYARLIWATSPIHAPPYGIMYDS 52
Query: 376 L 376
L
Sbjct: 51 L 49
>BG584442
Length = 775
Score = 52.8 bits (125), Expect = 4e-07
Identities = 39/109 (35%), Positives = 55/109 (49%)
Frame = +3
Query: 20 F*IIKDRIWKKNQLLK*SLSLASWKGGSH*VCFAIYPFLCDECFPSFGIPY**DRENVKL 79
F IKD I KN L+ + + S+ G+ *VC + FLCDE F *+ ++ +
Sbjct: 351 FSYIKDEI**KN*FLEKQMFVKSYVRGND*VCTPKHIFLCDEYFSPSKFSSR*N*KDYEY 530
Query: 80 LLMGS*FSEFSRIALAILGTSVSS*GFWRYVF*GS*GFQYSYGWQAGLE 128
MGS + + R AL +LG V +* WR+ F QYS W GL+
Sbjct: 531 FFMGSCWRKSQRNALDVLGEIVCT*KLWRHGFYRLHDVQYSNAW*TGLK 677
>TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non-LTR
retroelement reverse transcriptase {Oryza sativa
(japonica cultivar-group)}, partial (2%)
Length = 1262
Score = 51.6 bits (122), Expect = 9e-07
Identities = 34/100 (34%), Positives = 43/100 (43%), Gaps = 2/100 (2%)
Frame = +2
Query: 269 YSVKSAYTNILNHDVAVVHHRVPDNWTCIWSLKFPSKVKIFLWRACRNCLPTRIRLQSKG 328
YSVK Y I + V D +W PSKV +F+WR RN LPT+ L +G
Sbjct: 554 YSVKVFYRYITSTGHISDRSLVDD----VWHKHIPSKVSLFVWRLLRNRLPTKDNLVHRG 721
Query: 329 VQCTDK--CAVRDDFAEDSTHLFFMCNKSMICWQRSGLWI 366
V C +E +THLF CN W W+
Sbjct: 722 VLLATNAACVCGCVDSESTTHLFLHCNVFCSLWSLVRNWL 841
Score = 28.5 bits (62), Expect = 7.9
Identities = 14/49 (28%), Positives = 24/49 (48%)
Frame = -3
Query: 320 TRIRLQSKGVQCTDKCAVRDDFAEDSTHLFFMCNKSMICWQRSGLWILL 368
TR + + K +QC +C V + TH F+ ++ C + S + LL
Sbjct: 828 TRDQSEQKTLQCKKRCVVDSESTHPHTHAAFVARRTPRCTRLSFVGSLL 682
>BG586862
Length = 804
Score = 46.6 bits (109), Expect = 3e-05
Identities = 23/70 (32%), Positives = 33/70 (46%)
Frame = -1
Query: 297 IWSLKFPSKVKIFLWRACRNCLPTRIRLQSKGVQCTDKCAVRDDFAEDSTHLFFMCNKSM 356
+W +K + K FLWR N LP + L +G++C+ C + E HLF C +
Sbjct: 654 VWGIKTIPRHKSFLWRLLHNALPVKDELHKRGIRCSLLCPRCESKIETVQHLFLNCEVTQ 475
Query: 357 ICWQRSGLWI 366
W S L I
Sbjct: 474 KEWFGSQLGI 445
>CA919100 homologue to PIR|G90291|G902 endoglucanase precursor [imported] -
Sulfolobus solfataricus, partial (3%)
Length = 789
Score = 44.3 bits (103), Expect = 1e-04
Identities = 25/72 (34%), Positives = 33/72 (45%), Gaps = 2/72 (2%)
Frame = -2
Query: 297 IWSLKFPSKVKIFLWRACRNCLPTRIRLQSKGVQCTDK--CAVRDDFAEDSTHLFFMCNK 354
IW + P KV +F WR R+ LPT+ L +GV T+ C E + HLF C+
Sbjct: 584 IWHRQVPLKVSVFAWRLLRDRLPTKSNLIYRGVIPTEAGLCVSGCGALESAQHLFLSCSY 405
Query: 355 SMICWQRSGLWI 366
W WI
Sbjct: 404 FASLWSLVRDWI 369
>BG456581
Length = 683
Score = 41.2 bits (95), Expect = 0.001
Identities = 30/133 (22%), Positives = 51/133 (37%), Gaps = 2/133 (1%)
Frame = +2
Query: 267 GAYSVKSAYTNILNHDVAVVHHRVPDNW-TCIWSLKFPSKVKIFLWRACRNCLPTRIRLQ 325
G ++K Y+ + +H W + IW+ P WR + LPT L
Sbjct: 8 GKLTIKLVYSFLTSHTSCAP-------WASTIWNSCIPPSHSFICWRLAHDRLPTDDNLS 166
Query: 326 SKGVQCTDKCAVRDDFAEDSTHLFFMCNKSMICWQRSGLWILLMAIFYIDL-SFPTNVFA 384
S+G C+ + E S HLF C + W W+ +D SF + +
Sbjct: 167 SRGCALVSMCSFCLEQVETSDHLFLRCKFVVTLWS----WLCSQLRVGLDFSSFKALLSS 334
Query: 385 ILQHLDQQQKQVF 397
+ +H Q + ++
Sbjct: 335 LPRHCSSQVRDLY 373
>AW686588
Length = 567
Score = 40.8 bits (94), Expect = 0.002
Identities = 23/66 (34%), Positives = 28/66 (41%), Gaps = 2/66 (3%)
Frame = +1
Query: 303 PSKVKIFLWRACRNCLPTRIRLQSKGVQCTDK--CAVRDDFAEDSTHLFFMCNKSMICWQ 360
P KV I WR R+ LPT+ L + + C V AE + HLF C WQ
Sbjct: 145 PLKVSILAWRLIRDRLPTKANLVRRRCLAVEAAGCVVGCGIAETANHLFLHCATFGAVWQ 324
Query: 361 RSGLWI 366
WI
Sbjct: 325 HIRAWI 342
>BG585499
Length = 792
Score = 39.3 bits (90), Expect = 0.004
Identities = 30/116 (25%), Positives = 51/116 (43%)
Frame = +3
Query: 269 YSVKSAYTNILNHDVAVVHHRVPDNWTCIWSLKFPSKVKIFLWRACRNCLPTRIRLQSKG 328
+ +KS+Y N+L D ++V +W +W + P + + F+W C+ T R G
Sbjct: 165 FKIKSSY-NLLV*DQSIVDC----DWKMLWGWRGPHRTQTFMWLVAHGCILTNYRRSRWG 329
Query: 329 VQCTDKCAVRDDFAEDSTHLFFMCNKSMICWQRSGLWILLMAIFYIDLSFPTNVFA 384
+ C + D T L +C+ C S +WI L+ +I TN F+
Sbjct: 330 TRVLATCPCCGN--ADETVLHVLCD----CRPASQVWIRLVPSDWI-----TNFFS 464
>TC81772
Length = 982
Score = 38.9 bits (89), Expect = 0.006
Identities = 26/70 (37%), Positives = 36/70 (51%), Gaps = 1/70 (1%)
Frame = -1
Query: 290 VPDNWTCIWSLKFPSKVKIFLWRACRNCLPTRIRLQSKGVQCTDKCAVRDDFAE-DSTHL 348
V + W+ SLKF K K F+WR NCLP LQ++GV V + A +S +L
Sbjct: 352 VQNTWSI*RSLKF--KEKFFMWRFGSNCLPN*ANLQTRGVVTCPTVRVCCE*AT*NS*YL 179
Query: 349 FFMCNKSMIC 358
+ C +S C
Sbjct: 178 YLECTESAKC 149
>BF520135
Length = 202
Score = 38.1 bits (87), Expect = 0.010
Identities = 20/59 (33%), Positives = 27/59 (44%), Gaps = 2/59 (3%)
Frame = +3
Query: 297 IWSLKFPSKVKIFLWRACRNCLPTRIRLQSKGVQCTD--KCAVRDDFAEDSTHLFFMCN 353
+W + SKV IF WR LPT+ + +G+ D C R E HLF C+
Sbjct: 15 LWRKEDLSKVSIFAWRLFHGRLPTKANVFKRGIVHHDAHMCVTRCRLIESDVHLFLHCD 191
>TC82520
Length = 833
Score = 37.4 bits (85), Expect = 0.017
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +3
Query: 297 IWSLKFPSKVKIFLWRACRNCLPTRIRLQSKGV 329
+W PSKV +F+WR N LPT++ L + V
Sbjct: 186 VWQKNIPSKVSMFVWRLFHNRLPTKVNLMQRHV 284
>TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarotenoid
dioxygenase1 {Pisum sativum}, partial (43%)
Length = 1865
Score = 36.2 bits (82), Expect = 0.038
Identities = 28/104 (26%), Positives = 47/104 (44%), Gaps = 7/104 (6%)
Frame = -2
Query: 257 DVPV*KFERDGAYSVKSAYTNILNHDVAVVHHRVPDNWTCI----WSLKFPSKVKIFLWR 312
D+ V K +++G +SV S Y L ++ ++ R+ I W K P+KV F W
Sbjct: 448 DIWVWKPDKEGVFSVNSCY--FLLQNLRLLEDRLSYEEEVIFRELWKSKAPAKVLAFSWT 275
Query: 313 ACRNCLPTRIRLQSK---GVQCTDKCAVRDDFAEDSTHLFFMCN 353
+ +PT + L + V+ + +C E HLF C+
Sbjct: 274 LFLDRIPTMVNLGKRRLLRVEDSKRCVFCGCQDETVVHLFLHCD 143
>BE941032
Length = 435
Score = 35.0 bits (79), Expect = 0.085
Identities = 22/76 (28%), Positives = 31/76 (39%), Gaps = 6/76 (7%)
Frame = +2
Query: 308 IFLWRACRNCLPTRIRLQSKGV------QCTDKCAVRDDFAEDSTHLFFMCNKSMICWQR 361
IFLW N PT+ L +GV C +C D+THLF CN W
Sbjct: 149 IFLWCVLLNRFPTKDNLLKRGVISAIYQSCVGECGN----LYDATHLFLHCNFFRQIWIN 316
Query: 362 SGLWILLMAIFYIDLS 377
W+ + + + +S
Sbjct: 317 VSDWLSFVMVTLLRIS 364
>AL382240
Length = 320
Score = 34.7 bits (78), Expect = 0.11
Identities = 19/44 (43%), Positives = 24/44 (54%)
Frame = +1
Query: 456 GLNQVLADLNVMLMRPFLKLGIVLALVCVFEMMKVVMC*RRLNG 499
G NQ LA N +LM PF L LA CV M +++C + NG
Sbjct: 130 GRNQHLAXTNAILMHPFQLLLT*LAWACVLGMRMMLLCLQEQNG 261
>TC79628 similar to GP|17104799|gb|AAL34288.1 putative 2-nitropropane
dioxygenase {Arabidopsis thaliana}, partial (97%)
Length = 1560
Score = 33.1 bits (74), Expect = 0.32
Identities = 29/90 (32%), Positives = 44/90 (48%), Gaps = 1/90 (1%)
Frame = +2
Query: 482 VCVFEMMKVVMC*RRLNG*LRSLMWIWVRL*VYYQLCTGFVICSWVLWTLSLISKLS*IV 541
VCVFEM VMC +G + I RL* + C+G + W T I S +
Sbjct: 1160 VCVFEMQPEVMCLVEQHGLHHCVQ*ILTRL*DFVTRCSG*LTSVWTTSTSLWIQSSSLTL 1339
Query: 542 FMVVKMASL-F*RSNK*LYTPINF*FSNL* 570
+V+ +A+L F + + LY ++ F+N *
Sbjct: 1340 SVVIIVATLDFGKYH*LLYQLLSTHFTNF* 1429
>BE999296
Length = 384
Score = 31.6 bits (70), Expect = 0.94
Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 1/58 (1%)
Frame = -1
Query: 265 RDGAYS-VKSAYTNILNHDVAVVHHRVPDNWTCIWSLKFPSKVKIFLWRACRNCLPTR 321
R YS + AY +++ D + + +W P KV F+ R RNCLPT+
Sbjct: 327 RTATYS*IHGAYQFLMSADAPLDREYIDS----VWHNHIPLKVCFFVLRVLRNCLPTK 166
>BQ149324
Length = 374
Score = 28.9 bits (63), Expect = 6.1
Identities = 15/47 (31%), Positives = 24/47 (50%)
Frame = +3
Query: 318 LPTRIRLQSKGVQCTDKCAVRDDFAEDSTHLFFMCNKSMICWQRSGL 364
LPTR+RL+ K V C C + +ED+ F K + C + + +
Sbjct: 3 LPTRVRLKDKRVTCPMDCTLCTVGSEDTPSFNFSMLK*LKCLEYASI 143
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.364 0.161 0.620
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,045,041
Number of Sequences: 36976
Number of extensions: 383007
Number of successful extensions: 5715
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 1721
Number of HSP's successfully gapped in prelim test: 242
Number of HSP's that attempted gapping in prelim test: 3912
Number of HSP's gapped (non-prelim): 2152
length of query: 615
length of database: 9,014,727
effective HSP length: 102
effective length of query: 513
effective length of database: 5,243,175
effective search space: 2689748775
effective search space used: 2689748775
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.5 bits)
S2: 61 (28.1 bits)
Medicago: description of AC136953.11