BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136838.2 - phase: 2
(90 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BE204873 similar to GP|18767124|gb| unknown {Saccharomyces cerev... 190 9e-50
TC76421 homologue to PIR|T02995|T02995 unspecific monooxygenase ... 190 9e-50
TC76736 homologue to GP|22725683|gb|AAN04890.1 ribosomal protein... 49 1e-12
TC77668 similar to GP|18767124|gb|AAL79277.1 unknown {Saccharomy... 47 1e-10
TC76739 similar to GP|18767124|gb|AAL79277.1 unknown {Saccharomy... 54 1e-08
TC76322 similar to PIR|T02955|T02955 probable cytochrome P450 mo... 47 1e-06
TC76738 45 6e-06
AJ500300 similar to GP|18767124|gb| unknown {Saccharomyces cerev... 33 0.014
AW736496 weakly similar to PIR|G72580|G725 hypothetical protein ... 32 0.055
TC76888 similar to PIR|D75542|D75542 hypothetical protein - Dein... 27 1.4
TC78774 similar to GP|15292907|gb|AAK92824.1 unknown protein {Ar... 27 1.8
TC87635 27 1.8
AW685533 similar to GP|15081723|gb T10F18_70/T10F18_70 {Arabidop... 26 2.3
BF005386 26 3.0
TC90484 similar to GP|19310458|gb|AAL84964.1 AT4g17300/dl4685w {... 26 3.0
TC83642 similar to PIR|S52769|S52769 subtilisin-like proteinase ... 25 3.9
TC91551 similar to GP|16323095|gb|AAL15282.1 AT4g39900/T5J17_70 ... 25 3.9
AJ500810 25 5.1
TC82284 weakly similar to GP|11119510|gb|AAF37724.2 diaphanous p... 25 5.1
>BE204873 similar to GP|18767124|gb| unknown {Saccharomyces cerevisiae},
partial (30%)
Length = 574
Score = 190 bits (482), Expect = 9e-50
Identities = 88/90 (97%), Positives = 88/90 (97%)
Frame = -2
Query: 1 LGRNLPPDWGCIPKQPDSPTTPRGATGSEHNGALTLSGAPFQGTWARSAAEDASPYYNSN 60
LGRNLPPDWGCIPKQPDSPT PRGATGSEHNGALTLSGAPFQGTWARSAAEDASP YNSN
Sbjct: 282 LGRNLPPDWGCIPKQPDSPTAPRGATGSEHNGALTLSGAPFQGTWARSAAEDASPDYNSN 103
Query: 61 AEGDRFSWWAYPGSLAVTKGILVSFFSSAY 90
AEGDRFSWWAYPGSLAVTKGILVSFFSSAY
Sbjct: 102 AEGDRFSWWAYPGSLAVTKGILVSFFSSAY 13
>TC76421 homologue to PIR|T02995|T02995 unspecific monooxygenase (EC
1.14.14.1) - common tobacco, partial (44%)
Length = 5080
Score = 190 bits (482), Expect = 9e-50
Identities = 88/90 (97%), Positives = 88/90 (97%)
Frame = -3
Query: 1 LGRNLPPDWGCIPKQPDSPTTPRGATGSEHNGALTLSGAPFQGTWARSAAEDASPYYNSN 60
LGRNLPPDWGCIPKQPDSPT PRGATGSEHNGALTLSGAPFQGTWARSAAEDASP YNSN
Sbjct: 2507 LGRNLPPDWGCIPKQPDSPTAPRGATGSEHNGALTLSGAPFQGTWARSAAEDASPDYNSN 2328
Query: 61 AEGDRFSWWAYPGSLAVTKGILVSFFSSAY 90
AEGDRFSWWAYPGSLAVTKGILVSFFSSAY
Sbjct: 2327 AEGDRFSWWAYPGSLAVTKGILVSFFSSAY 2238
>TC76736 homologue to GP|22725683|gb|AAN04890.1 ribosomal protein L14 {Vigna
angularis}, partial (38%)
Length = 566
Score = 49.3 bits (116), Expect(2) = 1e-12
Identities = 29/60 (48%), Positives = 33/60 (54%)
Frame = +2
Query: 1 LGRNLPPDWGCIPKQPDSPTTPRGATGSEHNGALTLSGAPFQGTWARSAAEDASPYYNSN 60
L RNLPP C PKQ DS R A+ + NG LTL Q WA AE+AS YNS+
Sbjct: 233 LRRNLPPILSCNPKQLDS*RAYRRAS*GQPNGILTLHDTLSQRIWANRDAENASSNYNSD 412
Score = 37.7 bits (86), Expect(2) = 1e-12
Identities = 18/26 (69%), Positives = 21/26 (80%)
Frame = +1
Query: 65 RFSWWAYPGSLAVTKGILVSFFSSAY 90
RF A+P SLAVT+GI+V FFSSAY
Sbjct: 427 RFKI*AFPSSLAVTRGIIVIFFSSAY 504
>TC77668 similar to GP|18767124|gb|AAL79277.1 unknown {Saccharomyces
cerevisiae}, partial (26%)
Length = 362
Score = 47.4 bits (111), Expect(2) = 1e-10
Identities = 25/37 (67%), Positives = 27/37 (72%)
Frame = +1
Query: 54 SPYYNSNAEGDRFSWWAYPGSLAVTKGILVSFFSSAY 90
S YNS E RF A+P SLAVT+GILVSFFSSAY
Sbjct: 208 SSKYNSTWEPGRFKI*AFPSSLAVTRGILVSFFSSAY 318
Score = 32.7 bits (73), Expect(2) = 1e-10
Identities = 20/44 (45%), Positives = 23/44 (51%)
Frame = +3
Query: 4 NLPPDWGCIPKQPDSPTTPRGATGSEHNGALTLSGAPFQGTWAR 47
+LPP+ CIPKQ DS T G +G LTL FQ T R
Sbjct: 57 SLPPNLSCIPKQLDS*KTNIKYLGLIMHGILTLHDVLFQRT*TR 188
>TC76739 similar to GP|18767124|gb|AAL79277.1 unknown {Saccharomyces
cerevisiae}, partial (29%)
Length = 371
Score = 53.9 bits (128), Expect = 1e-08
Identities = 42/94 (44%), Positives = 48/94 (50%), Gaps = 4/94 (4%)
Frame = +3
Query: 1 LGRNLPPDWGCIPKQPDSPTTPRGAT-GSEHNGALTLSGAPFQGTWARSAAEDASPYYNS 59
L RNLPP C PKQ DS T R A+ NG LTL Q A ++
Sbjct: 39 LRRNLPPILSCNPKQLDSWKTYRRASLRLTMNGILTLHDTLSQRI*ANPGCRKR--FFKI 212
Query: 60 N---AEGDRFSWWAYPGSLAVTKGILVSFFSSAY 90
E +RF AYP SLAVT+GI+V FFSSAY
Sbjct: 213 QLC*VEPNRFKI*AYPSSLAVTEGIIVIFFSSAY 314
>TC76322 similar to PIR|T02955|T02955 probable cytochrome P450 monooxygenase -
maize (fragment), partial (60%)
Length = 3098
Score = 47.0 bits (110), Expect = 1e-06
Identities = 33/65 (50%), Positives = 35/65 (53%), Gaps = 1/65 (1%)
Frame = +1
Query: 27 GSEHNGALTLSGAPFQGTWARSAAEDASPYY-NSNAEGDRFSWWAYPGSLAVTKGILVSF 85
G + GALTL G PFQGT R A P + A G RF A SLAVT I V F
Sbjct: 2629 GVQPYGALTLYGVPFQGT--RKAPRPKHPLQITTRALGARFQI*AVAASLAVTGAIPVGF 2802
Query: 86 FSSAY 90
FSSAY
Sbjct: 2803 FSSAY 2817
>TC76738
Length = 368
Score = 44.7 bits (104), Expect = 6e-06
Identities = 27/61 (44%), Positives = 34/61 (55%), Gaps = 1/61 (1%)
Frame = +2
Query: 31 NGALTLSGAPFQGTWA-RSAAEDASPYYNSNAEGDRFSWWAYPGSLAVTKGILVSFFSSA 89
+G +TL A F TWA + + + DRF A P SLAVT+GI+V FFSSA
Sbjct: 128 DGIITLHDASFNRTWADQKRRKRFFKLQLDRVKPDRFKI*ACPSSLAVTRGIIVIFFSSA 307
Query: 90 Y 90
Y
Sbjct: 308 Y 310
Score = 30.0 bits (66), Expect = 0.16
Identities = 25/60 (41%), Positives = 27/60 (44%), Gaps = 1/60 (1%)
Frame = +3
Query: 1 LGRNLPPDWGCIPKQPDSPTTPRGATGSEHNGALTLSGAPFQ-GTWARSAAEDASPYYNS 59
L RNLPP C PKQ DS R G L+ S P G AE+AS YNS
Sbjct: 39 LRRNLPPILSCNPKQLDS*KAYRKTQMINRTG-LSPSMMPHSIGLGLTKNAENASSNYNS 215
>AJ500300 similar to GP|18767124|gb| unknown {Saccharomyces cerevisiae},
partial (16%)
Length = 388
Score = 33.5 bits (75), Expect = 0.014
Identities = 15/21 (71%), Positives = 17/21 (80%)
Frame = +3
Query: 70 AYPGSLAVTKGILVSFFSSAY 90
A+P SLAVTKGI+ FFSS Y
Sbjct: 273 AFPSSLAVTKGIIFIFFSSPY 335
>AW736496 weakly similar to PIR|G72580|G725 hypothetical protein APE1926 -
Aeropyrum pernix (strain K1), partial (20%)
Length = 645
Score = 31.6 bits (70), Expect = 0.055
Identities = 15/22 (68%), Positives = 15/22 (68%)
Frame = -1
Query: 69 WAYPGSLAVTKGILVSFFSSAY 90
WA P SLA T GI FFSSAY
Sbjct: 195 WANPRSLATTYGITFVFFSSAY 130
>TC76888 similar to PIR|D75542|D75542 hypothetical protein - Deinococcus
radiodurans (strain R1), partial (57%)
Length = 1749
Score = 26.9 bits (58), Expect = 1.4
Identities = 13/21 (61%), Positives = 14/21 (65%)
Frame = +2
Query: 70 AYPGSLAVTKGILVSFFSSAY 90
A P SLA T GI +FFSS Y
Sbjct: 1511 AVPISLAATTGIAFAFFSSGY 1573
>TC78774 similar to GP|15292907|gb|AAK92824.1 unknown protein {Arabidopsis
thaliana}, partial (61%)
Length = 1375
Score = 26.6 bits (57), Expect = 1.8
Identities = 22/76 (28%), Positives = 29/76 (37%), Gaps = 11/76 (14%)
Frame = +1
Query: 13 PKQPDSPTTPRGATGSEHNGALTLSGA------PFQGTWARSAAEDASPYYNS-----NA 61
P P PTT +G +G L SGA P + T + A P +N N
Sbjct: 688 PLPPVLPTTGLITSGPISSGPLNSSGAPRKVSGPLEATGSMKLQGSAVPVHNQAVTVLNQ 867
Query: 62 EGDRFSWWAYPGSLAV 77
F+W Y G A+
Sbjct: 868 VAASFTWNTYWGRRAI 915
>TC87635
Length = 806
Score = 26.6 bits (57), Expect = 1.8
Identities = 13/44 (29%), Positives = 19/44 (42%)
Frame = -3
Query: 5 LPPDWGCIPKQPDSPTTPRGATGSEHNGALTLSGAPFQGTWARS 48
+ P C P + + + PRG H+ L L P +W RS
Sbjct: 432 MTPGLSCTPLEGRAESRPRGEEELPHHANLMLQCLPHLLSWQRS 301
>AW685533 similar to GP|15081723|gb T10F18_70/T10F18_70 {Arabidopsis
thaliana}, partial (19%)
Length = 407
Score = 26.2 bits (56), Expect = 2.3
Identities = 12/12 (100%), Positives = 12/12 (100%)
Frame = -2
Query: 77 VTKGILVSFFSS 88
VTKGILVSFFSS
Sbjct: 61 VTKGILVSFFSS 26
>BF005386
Length = 504
Score = 25.8 bits (55), Expect = 3.0
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +3
Query: 17 DSPTTPRGATGSEHNGALTLSGAPFQGTWARSAAEDASPY 56
D PTT ++ +S P QGTW + +++ +PY
Sbjct: 123 DQPTTSYNLMNTD-----AISNLPEQGTWDQYNSDNCAPY 227
>TC90484 similar to GP|19310458|gb|AAL84964.1 AT4g17300/dl4685w {Arabidopsis
thaliana}, partial (24%)
Length = 722
Score = 25.8 bits (55), Expect = 3.0
Identities = 21/58 (36%), Positives = 26/58 (44%)
Frame = -1
Query: 31 NGALTLSGAPFQGTWARSAAEDASPYYNSNAEGDRFSWWAYPGSLAVTKGILVSFFSS 88
N LTL+ AP AR A + N+ F W GSL T IL+SF S+
Sbjct: 653 NAFLTLATAPKVFVLARRCA-----FVLKNSLLTLFFWMGKEGSLLPTSTILLSFNST 495
>TC83642 similar to PIR|S52769|S52769 subtilisin-like proteinase ag12 (EC
3.4.21.-) - alder, partial (30%)
Length = 1177
Score = 25.4 bits (54), Expect = 3.9
Identities = 12/49 (24%), Positives = 21/49 (42%)
Frame = +2
Query: 37 SGAPFQGTWARSAAEDASPYYNSNAEGDRFSWWAYPGSLAVTKGILVSF 85
S FQ T+ + A+ YY+S + W P +A +L ++
Sbjct: 422 SSIKFQQTFVGTKPAPAAAYYSSRGPSHSYPWILKPDIMAPGSRVLAAY 568
>TC91551 similar to GP|16323095|gb|AAL15282.1 AT4g39900/T5J17_70
{Arabidopsis thaliana}, partial (22%)
Length = 1089
Score = 25.4 bits (54), Expect = 3.9
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +3
Query: 17 DSPTTPRGATGSEHNGALTLSGAPFQGTWARSAA 50
+ + PRG GS+ ++S P +G ARSA+
Sbjct: 261 EETSLPRGLHGSQAQNVYSISMVPKEGENARSAS 362
>AJ500810
Length = 355
Score = 25.0 bits (53), Expect = 5.1
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = +3
Query: 74 SLAVTKGILVSFF 86
SLAVTK ILVSFF
Sbjct: 144 SLAVTKDILVSFF 182
>TC82284 weakly similar to GP|11119510|gb|AAF37724.2 diaphanous protein
{Entamoeba histolytica}, partial (4%)
Length = 671
Score = 25.0 bits (53), Expect = 5.1
Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 3/40 (7%)
Frame = +3
Query: 31 NGALTLSGAPFQGTWARSAAEDASPYYNS---NAEGDRFS 67
N + T G TW +A+ ASP+++ NA RFS
Sbjct: 180 NASTTSYGCKRS*TWCDAASSTASPHWDQTSINATSTRFS 299
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.313 0.132 0.435
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,038,967
Number of Sequences: 36976
Number of extensions: 36433
Number of successful extensions: 170
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of query: 90
length of database: 9,014,727
effective HSP length: 66
effective length of query: 24
effective length of database: 6,574,311
effective search space: 157783464
effective search space used: 157783464
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC136838.2