BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136504.7 + phase: 0
(261 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BE998749 similar to PIR|T04703|T047 hypothetical protein F4B14.2... 432 e-130
TC77451 weakly similar to PIR|T05159|T05159 hypothetical protein... 66 2e-11
TC77452 homologue to PIR|T05159|T05159 hypothetical protein F18E... 33 0.11
TC76341 similar to GP|7528270|gb|AAF63202.1| poly(A)-binding pro... 31 0.42
BQ137253 similar to GP|18308125|gb bromodomain-containing protei... 31 0.55
TC87707 similar to PIR|B84632|B84632 probable chloroplast initia... 29 1.6
BQ750926 similar to PIR|T51049|T510 related to nucleolar phospho... 29 2.1
BQ144502 weakly similar to GP|6523547|emb hydroxyproline-rich gl... 29 2.1
TC79771 similar to GP|20466574|gb|AAM20604.1 putative protein {A... 28 2.7
BQ144482 similar to GP|6523547|emb| hydroxyproline-rich glycopro... 28 2.7
TC77430 similar to GP|13676413|dbj|BAB41197. hypothetical protei... 28 3.6
TC79502 similar to GP|19881712|gb|AAM01113.1 Unknown protein {Or... 28 4.6
CA917370 similar to GP|15010620|gb AT4g08960/T3H13_2 {Arabidopsi... 27 6.1
AW696997 similar to GP|21593548|gb unknown {Arabidopsis thaliana... 27 6.1
TC80433 similar to GP|8778710|gb|AAF79718.1| T1N15.2 {Arabidopsi... 27 6.1
TC88220 similar to GP|15293201|gb|AAK93711.1 unknown protein {Ar... 27 6.1
TC79659 similar to GP|15010620|gb|AAK73969.1 AT4g08960/T3H13_2 {... 27 6.1
BE324230 27 6.1
BQ751412 similar to GP|16944504|emb| putative protein {Neurospor... 27 7.9
BE997678 27 7.9
>BE998749 similar to PIR|T04703|T047 hypothetical protein F4B14.200 -
Arabidopsis thaliana, partial (30%)
Length = 818
Score = 432 bits (1110), Expect(2) = e-130
Identities = 214/217 (98%), Positives = 215/217 (98%)
Frame = +1
Query: 1 MTTTEKRKGRRKLKKSGTKYLKPGTLAQLRCSKYLGSGSGAGRVCNDIGKKRVELLNLEK 60
MTTTEKRKGRRKLKKSGTKYLKPGTLAQLRCSKYLGSGSGAGRVCNDIGKKRVELLNLEK
Sbjct: 106 MTTTEKRKGRRKLKKSGTKYLKPGTLAQLRCSKYLGSGSGAGRVCNDIGKKRVELLNLEK 285
Query: 61 NEDHEGKVFDKSPLMLSPVNLVKQSSSVGTPKTPRIDECQSESRLESLPIDLLVKILCCL 120
NEDHEGKVFDKSPLMLSPVNLVKQSSSVGTPKTPRIDECQSESRLESLPIDLLVKILCCL
Sbjct: 286 NEDHEGKVFDKSPLMLSPVNLVKQSSSVGTPKTPRIDECQSESRLESLPIDLLVKILCCL 465
Query: 121 HHDQLRAVFHVSQRIRKAVIVARQFHFNYTTPDRSRLEMLSTMTPRPSEHWPFLCRGDGK 180
HHDQLRAVFHVSQRIRKAVIVARQFHFNYTTPDRSRLEMLSTMTPRPSEHWPFL GDGK
Sbjct: 466 HHDQLRAVFHVSQRIRKAVIVARQFHFNYTTPDRSRLEMLSTMTPRPSEHWPFLW-GDGK 642
Query: 181 GLRIPSPHTPKAPRHGPRPPSRLKVSEMRQVTAVLFK 217
GLRIPSPHTPKAPRHGPRPPSRLKVSEMRQVTAVLF+
Sbjct: 643 GLRIPSPHTPKAPRHGPRPPSRLKVSEMRQVTAVLFR 753
Score = 51.6 bits (122), Expect(2) = e-130
Identities = 23/24 (95%), Positives = 24/24 (99%)
Frame = +3
Query: 216 FKESAFASRCMVPSVIPKPQCKSV 239
F+ESAFASRCMVPSVIPKPQCKSV
Sbjct: 747 FQESAFASRCMVPSVIPKPQCKSV 818
>TC77451 weakly similar to PIR|T05159|T05159 hypothetical protein F18E5.130
- Arabidopsis thaliana, partial (28%)
Length = 1049
Score = 65.9 bits (159), Expect = 2e-11
Identities = 40/114 (35%), Positives = 65/114 (56%)
Frame = +1
Query: 48 IGKKRVELLNLEKNEDHEGKVFDKSPLMLSPVNLVKQSSSVGTPKTPRIDECQSESRLES 107
+G+KRV + N ++ F+ +P+ V L + S R D +SRLE+
Sbjct: 280 LGRKRVVVSNNDE-------AFESNPVT---VPLKRMCSG-------RFDTISEKSRLEA 408
Query: 108 LPIDLLVKILCCLHHDQLRAVFHVSQRIRKAVIVARQFHFNYTTPDRSRLEMLS 161
LP DLLV++LC + HD L +F+VS I++A +A+Q HF ++TP +S + + S
Sbjct: 409 LPQDLLVRVLCGVDHDDLEQLFNVSTTIKEAGEIAKQMHFEFSTPKKSSVAVRS 570
>TC77452 homologue to PIR|T05159|T05159 hypothetical protein F18E5.130 -
Arabidopsis thaliana, partial (7%)
Length = 886
Score = 33.1 bits (74), Expect = 0.11
Identities = 34/129 (26%), Positives = 52/129 (39%), Gaps = 35/129 (27%)
Frame = +2
Query: 48 IGKKRVELLNLEKNEDHEGKVFDKSPLMLSPVNLVKQSSSVGTPKTPRIDECQSESRLES 107
+G+KRV + N ++ F+ +P+ V L + S R D +SRLE+
Sbjct: 239 LGRKRVVVSNNDE-------AFESNPVT---VPLKRMCSG-------RFDTISEKSRLEA 367
Query: 108 LPIDLLV-----------------------------------KILCCLHHDQLRAVFHVS 132
LP DLLV ++LC + HD L +F+VS
Sbjct: 368 LPQDLLVSFAINFTV*FTIFHFRFSI*FC*IFYFIFLIFLQVRVLCGVDHDDLEQLFNVS 547
Query: 133 QRIRKAVIV 141
I++AV V
Sbjct: 548 TTIKEAVSV 574
>TC76341 similar to GP|7528270|gb|AAF63202.1| poly(A)-binding protein {Cucumis
sativus}, partial (90%)
Length = 2501
Score = 31.2 bits (69), Expect = 0.42
Identities = 18/61 (29%), Positives = 29/61 (47%)
Frame = -1
Query: 66 GKVFDKSPLMLSPVNLVKQSSSVGTPKTPRIDECQSESRLESLPIDLLVKILCCLHHDQL 125
G+ F K + L P+ V+ + KT EC S PID +++L L +DQ+
Sbjct: 2486 GEFFFKMMIYLRPITWVQSKHIPKSTKTVN*KECVSIQE*NKQPIDP*IQLLVILKYDQI 2307
Query: 126 R 126
+
Sbjct: 2306 K 2304
>BQ137253 similar to GP|18308125|gb bromodomain-containing protein BRD4 long
variant {Mus musculus}, partial (1%)
Length = 1033
Score = 30.8 bits (68), Expect = 0.55
Identities = 30/100 (30%), Positives = 36/100 (36%), Gaps = 11/100 (11%)
Frame = -1
Query: 173 FLCRGDGKGLRIPSPHTPKAPRHGPRPP------SRLKVSEMRQVTAVLFKESAFASRCM 226
FLC GD P P TP PR P PP L + E V +L+ S
Sbjct: 328 FLC-GDPLFTPFPPPSTPLLPRPSPPPPPPGPQTGALSMGESFSVILLLWTGSVIWIPGG 152
Query: 227 VPSVIPKPQC-----KSVASNRVLFCEDELCKAVAQNKLL 261
P +P S + R LFC L + Q LL
Sbjct: 151 TPMSAQRPNATEG*PPSSSFRRCLFCFVSLGRVSGQEALL 32
>TC87707 similar to PIR|B84632|B84632 probable chloroplast initiation factor
3 [imported] - Arabidopsis thaliana, partial (55%)
Length = 1249
Score = 29.3 bits (64), Expect = 1.6
Identities = 13/42 (30%), Positives = 23/42 (53%)
Frame = +2
Query: 75 MLSPVNLVKQSSSVGTPKTPRIDECQSESRLESLPIDLLVKI 116
+L P V S + KTP +D+ + L +P+DLL+++
Sbjct: 194 VLPPTVAVATSEELLLRKTPTMDKLSTSPHLVRIPLDLLIRV 319
>BQ750926 similar to PIR|T51049|T510 related to nucleolar phosphoprotein
[imported] - Neurospora crassa, partial (9%)
Length = 779
Score = 28.9 bits (63), Expect = 2.1
Identities = 12/20 (60%), Positives = 15/20 (75%), Gaps = 1/20 (5%)
Frame = +1
Query: 184 IPSPHTPKAPRH-GPRPPSR 202
IPSPH P++ +H PRPP R
Sbjct: 499 IPSPHLPRSLKHMHPRPPQR 558
>BQ144502 weakly similar to GP|6523547|emb hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (39%)
Length = 1358
Score = 28.9 bits (63), Expect = 2.1
Identities = 18/39 (46%), Positives = 18/39 (46%), Gaps = 3/39 (7%)
Frame = -3
Query: 165 PRPSEHWP--FLC-RGDGKGLRIPSPHTPKAPRHGPRPP 200
PRP H P C RG R P P P P GPRPP
Sbjct: 264 PRPRPHSP*QAACGRGHPPRHRPPPPGPPPPPPLGPRPP 148
>TC79771 similar to GP|20466574|gb|AAM20604.1 putative protein {Arabidopsis
thaliana}, partial (30%)
Length = 1431
Score = 28.5 bits (62), Expect = 2.7
Identities = 18/56 (32%), Positives = 28/56 (49%)
Frame = +1
Query: 31 CSKYLGSGSGAGRVCNDIGKKRVELLNLEKNEDHEGKVFDKSPLMLSPVNLVKQSS 86
C K L +G ++I + +V LLN+++N E K PL+L V+ SS
Sbjct: 529 CKKDLFEKAGMESPLSEILEIKVPLLNIDENIIQENKPLPDMPLLLKSVSSGSLSS 696
>BQ144482 similar to GP|6523547|emb| hydroxyproline-rich glycoprotein DZ-HRGP
{Volvox carteri f. nagariensis}, partial (16%)
Length = 993
Score = 28.5 bits (62), Expect = 2.7
Identities = 20/55 (36%), Positives = 22/55 (39%), Gaps = 3/55 (5%)
Frame = +3
Query: 164 TPRPSEHWPFLCRGDGKGLRIPSPHTPKAPRHGPRP---PSRLKVSEMRQVTAVL 215
+P P P L G GLR P P P P P P PSR V R+ L
Sbjct: 267 SPPPPPPQPLL----GPGLRPPGPRPPAPPPPAPAPRPAPSRPSVGRPRRAAPSL 419
>TC77430 similar to GP|13676413|dbj|BAB41197. hypothetical protein {Glycine
max}, partial (71%)
Length = 2529
Score = 28.1 bits (61), Expect = 3.6
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -2
Query: 185 PSPHTPKAPRHGPRPP 200
P P PRHGPRPP
Sbjct: 1964 PRARPPPPPRHGPRPP 1917
>TC79502 similar to GP|19881712|gb|AAM01113.1 Unknown protein {Oryza sativa}
[Oryza sativa (japonica cultivar-group)], partial (8%)
Length = 720
Score = 27.7 bits (60), Expect = 4.6
Identities = 12/24 (50%), Positives = 13/24 (54%)
Frame = -1
Query: 165 PRPSEHWPFLCRGDGKGLRIPSPH 188
P P H+ LC G G IPSPH
Sbjct: 303 PSPHLHYHSLCYQIGHGRHIPSPH 232
>CA917370 similar to GP|15010620|gb AT4g08960/T3H13_2 {Arabidopsis thaliana},
partial (26%)
Length = 604
Score = 27.3 bits (59), Expect = 6.1
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +3
Query: 185 PSPHTPKAPRHGPRPPSRLKVS 206
P P +PK+P H P PS L+ S
Sbjct: 192 PPPTSPKSPHHQPTVPSALRRS 257
>AW696997 similar to GP|21593548|gb unknown {Arabidopsis thaliana}, partial
(21%)
Length = 742
Score = 27.3 bits (59), Expect = 6.1
Identities = 22/66 (33%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = -1
Query: 182 LRIPSPHTPKAPRHGPRPPSRLKVSEMRQVTAVLFKESAFASRCMVPS--VIPKPQCKSV 239
L++PSPH + RP +R S Q+ +A ASR PS P P S
Sbjct: 661 LKLPSPHMACSRHALRRPRTRHGRSRSTQLLVCATHLAAAASRTTSPSPHKSPTPHAASA 482
Query: 240 ASNRVL 245
AS+ L
Sbjct: 481 ASSLYL 464
>TC80433 similar to GP|8778710|gb|AAF79718.1| T1N15.2 {Arabidopsis
thaliana}, partial (13%)
Length = 794
Score = 27.3 bits (59), Expect = 6.1
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = -2
Query: 172 PFLCRGDGKGLRIPSPHTPKAPRHGPRPP 200
P+ C G + R P P P+ P PRPP
Sbjct: 97 PYCCWGIPRPPRPPPP*APRPPP*SPRPP 11
>TC88220 similar to GP|15293201|gb|AAK93711.1 unknown protein {Arabidopsis
thaliana}, partial (26%)
Length = 1388
Score = 27.3 bits (59), Expect = 6.1
Identities = 20/74 (27%), Positives = 31/74 (41%), Gaps = 3/74 (4%)
Frame = -3
Query: 134 RIRKAVIVARQFHFNYTTPDRSRLEMLSTMTPRPSEHWPFLCRGDGKGLRIPSPHTPKAP 193
R + AVI+ + T PD+S L LS++T P +C +P AP
Sbjct: 726 RFKSAVILLKSCDIA*T*PDKSCLTALSSLTS------PRICCSSALATSVPRSSASNAP 565
Query: 194 R---HGPRPPSRLK 204
+ P P R++
Sbjct: 564 AFSYNSPHPHLRIR 523
>TC79659 similar to GP|15010620|gb|AAK73969.1 AT4g08960/T3H13_2 {Arabidopsis
thaliana}, partial (82%)
Length = 1507
Score = 27.3 bits (59), Expect = 6.1
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +1
Query: 185 PSPHTPKAPRHGPRPPSRLKVS 206
P P +PK+P H P PS L+ S
Sbjct: 163 PPPTSPKSPHHQPTVPSALRRS 228
>BE324230
Length = 313
Score = 27.3 bits (59), Expect = 6.1
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +1
Query: 19 KYLKPGTLAQLRCSK 33
K+LKPGTLA+LR SK
Sbjct: 121 KFLKPGTLARLRDSK 165
>BQ751412 similar to GP|16944504|emb| putative protein {Neurospora crassa},
partial (72%)
Length = 704
Score = 26.9 bits (58), Expect = 7.9
Identities = 14/49 (28%), Positives = 24/49 (48%)
Frame = +1
Query: 194 RHGPRPPSRLKVSEMRQVTAVLFKESAFASRCMVPSVIPKPQCKSVASN 242
RHG P L + +RQ + +SA A RCM +++ + ++N
Sbjct: 304 RHGSHRPQPLLLRALRQDGRLQMSDSAAAYRCMEWTLLNNVNMNNTSTN 450
>BE997678
Length = 454
Score = 26.9 bits (58), Expect = 7.9
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -1
Query: 29 LRCSKYLGSGSGAGRVCNDIG 49
+RC Y G G+G GR D+G
Sbjct: 340 IRCQVYKGRGTGGGRSTPDVG 278
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.135 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,870,629
Number of Sequences: 36976
Number of extensions: 140687
Number of successful extensions: 1034
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 983
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1028
length of query: 261
length of database: 9,014,727
effective HSP length: 94
effective length of query: 167
effective length of database: 5,538,983
effective search space: 925010161
effective search space used: 925010161
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC136504.7