BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136504.6 - phase: 0 /pseudo
(417 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CA990754 similar to GP|21397269|g Unknown protein {Oryza sativa ... 324 3e-89
BG645929 similar to GP|22137178|gb AT4g35920/F4B14_190 {Arabidop... 94 6e-36
TC80164 similar to PIR|A84670|A84670 probable nucleotide-binding... 32 0.35
TC87503 similar to SP|Q43111|PME3_PHAVU Pectinesterase 3 precurs... 32 0.59
AW208057 similar to PIR|F96745|F967 unknown protein T9N14.4 [imp... 32 0.59
TC89448 similar to PIR|C84864|C84864 hypothetical protein At2g43... 32 0.59
BG584702 weakly similar to PIR|T04811|T048 STIG1 protein homolog... 31 1.0
BF520164 weakly similar to GP|21593762|gb| unknown {Arabidopsis ... 26 2.2
BG454439 similar to GP|14517399|gb At2g32810/F24L7.5 {Arabidopsi... 30 2.3
TC87372 similar to GP|14091469|gb|AAK53758.1 putative potassium ... 30 2.3
TC76625 similar to SP|O81363|RS4_PRUAR 40S ribosomal protein S4.... 29 2.9
TC83638 similar to GP|19698943|gb|AAL91207.1 unknown protein {Ar... 29 2.9
TC89297 weakly similar to GP|9294431|dbj|BAB02551.1 gene_id:MMB1... 29 3.8
TC83396 similar to GP|22858664|gb|AAN05792.1 unknown {Gossypium ... 28 5.0
TC88969 similar to GP|17381224|gb|AAL36424.1 unknown protein {Ar... 28 6.6
TC80576 similar to GP|11139266|gb|AAG31651.1 PRLI-interacting fa... 28 8.6
>CA990754 similar to GP|21397269|g Unknown protein {Oryza sativa (japonica
cultivar-group)}, partial (15%)
Length = 700
Score = 324 bits (831), Expect = 3e-89
Identities = 168/223 (75%), Positives = 188/223 (83%)
Frame = +3
Query: 52 LIGNLLDQLKISELKKYPETREPLEQLEDALRRSYILVNSCQDRSYLYLLAMGWNIVYQF 111
LIGNLL+QLKISELK+YPETREPLEQLEDALRRSY+LV+SCQDRSYLYLLAMGWNIVYQF
Sbjct: 3 LIGNLLEQLKISELKRYPETREPLEQLEDALRRSYMLVHSCQDRSYLYLLAMGWNIVYQF 182
Query: 112 RKAQNEIDRYLRLVPLITLVDNARATRVRERLEVIEKDQCAYTLDDEDQKVQTVFMKPEH 171
RKAQNEIDRYLRLVPLITLVDNAR VRERLEVIE DQ YTLDDEDQ+ TV +KP+
Sbjct: 183 RKAQNEIDRYLRLVPLITLVDNAR---VRERLEVIEHDQREYTLDDEDQQAHTVILKPDP 353
Query: 172 DKEDTVVLKKTLSCSYPNFSFTEALKKENEKLQLELHHSQANMDINQCEFIQRLLDVTNF 231
DKEDT VLKKTLSCSYPN +F EA+KKE EKL LEL SQAN+D+NQCE IQRLL+VT
Sbjct: 354 DKEDTAVLKKTLSCSYPNCTFPEAIKKEKEKLNLELQRSQANLDMNQCEVIQRLLEVTKV 533
Query: 232 AAYSLPEKLSPEDNYQIVEYSNNSHSDANGSKGHSSNEKHHKK 274
A YSLP+K SPE ++ + N S+SD+N K HSS+E +K
Sbjct: 534 AEYSLPDKCSPEKSH---KKENYSYSDSNDDKVHSSDENTIRK 653
>BG645929 similar to GP|22137178|gb AT4g35920/F4B14_190 {Arabidopsis
thaliana}, partial (24%)
Length = 676
Score = 94.4 bits (233), Expect(2) = 6e-36
Identities = 49/60 (81%), Positives = 51/60 (84%), Gaps = 7/60 (11%)
Frame = -2
Query: 257 SDANGSKGHSSNEKHHKK-------KPKFQKRIRCQLEVHISRKIGILIYLLVVLNLIFI 309
SDANGSKGHS NEK HKK +PKFQKRIRCQLEVHISRKIGILIYLLVVLNLIF+
Sbjct: 675 SDANGSKGHSFNEKPHKKSDIFHHQEPKFQKRIRCQLEVHISRKIGILIYLLVVLNLIFV 496
Score = 74.7 bits (182), Expect(2) = 6e-36
Identities = 36/36 (100%), Positives = 36/36 (100%)
Frame = -3
Query: 337 IFCRSMQ*LYCIFVGFILLLLYLLHKEEA*EDDEHS 372
IFCRSMQ*LYCIFVGFILLLLYLLHKEEA*EDDEHS
Sbjct: 428 IFCRSMQ*LYCIFVGFILLLLYLLHKEEA*EDDEHS 321
Score = 55.8 bits (133), Expect = 3e-08
Identities = 39/90 (43%), Positives = 43/90 (47%), Gaps = 21/90 (23%)
Frame = -1
Query: 315 IKTFFYPCGTFSKIATVATNRPI---------FCRSMQ*LYCIFVGFILLLLYLLHKEEA 365
IKTFFYPCGTFSKIATVATNRPI S+ C + I L +
Sbjct: 496 IKTFFYPCGTFSKIATVATNRPISSAEVCNDFIAYSLVLSCCCYTCCIRRKLRKMMNIPG 317
Query: 366 *EDDEH------------SEWREVQIRGVY 383
D+ EWREVQIRGVY
Sbjct: 316 GYVDDFLSHLMCCSCALVQEWREVQIRGVY 227
>TC80164 similar to PIR|A84670|A84670 probable nucleotide-binding protein
[imported] - Arabidopsis thaliana, partial (31%)
Length = 1215
Score = 32.3 bits (72), Expect = 0.35
Identities = 23/113 (20%), Positives = 43/113 (37%)
Frame = +3
Query: 169 PEHDKEDTVVLKKTLSCSYPNFSFTEALKKENEKLQLELHHSQANMDINQCEFIQRLLDV 228
P+ + +LK + P++ L + + H Q N ++ I+ V
Sbjct: 378 PDETRASRQILKDYIDGKLPHYEMPPGLSTQELASEDSNEHDQVNPHVSDASDIEDSSVV 557
Query: 229 TNFAAYSLPEKLSPEDNYQIVEYSNNSHSDANGSKGHSSNEKHHKKKPKFQKR 281
A L L D+ + +N S+ K ++KHH+K P+ + R
Sbjct: 558 ETELAPKLEHVL---DDLSSFDMANGLASNKVAPKKTKESQKHHRKPPRTKNR 707
>TC87503 similar to SP|Q43111|PME3_PHAVU Pectinesterase 3 precursor (EC
3.1.1.11) (Pectin methylesterase 3) (PE 3)., partial
(93%)
Length = 2088
Score = 31.6 bits (70), Expect = 0.59
Identities = 18/72 (25%), Positives = 37/72 (51%), Gaps = 2/72 (2%)
Frame = +1
Query: 197 KKENEKLQLELHHSQANMDINQCEFIQRLLDVTNFAAYSLPEK--LSPEDNYQIVEYSNN 254
K+++ KLQL+ H++ N + E QRL S+P+K + ++ ++ +S++
Sbjct: 1 KQQHNKLQLQKRHTKQNNNNGYYEIFQRLWQSRRNRTTSIPKKDT*THHNHNHLLHHSSS 180
Query: 255 SHSDANGSKGHS 266
SH + H+
Sbjct: 181 SHHSCSCRNPHT 216
>AW208057 similar to PIR|F96745|F967 unknown protein T9N14.4 [imported] -
Arabidopsis thaliana, partial (8%)
Length = 421
Score = 31.6 bits (70), Expect = 0.59
Identities = 32/120 (26%), Positives = 50/120 (41%)
Frame = +2
Query: 172 DKEDTVVLKKTLSCSYPNFSFTEALKKENEKLQLELHHSQANMDINQCEFIQRLLDVTNF 231
++E ++ L+ TL YP FT K++ L L S+AN D + F
Sbjct: 77 ERERSMALEPTLL--YPQDPFTYGFNKDHYNYNL-LTDSEANFDYTNFNLQN---EEQGF 238
Query: 232 AAYSLPEKLSPEDNYQIVEYSNNSHSDANGSKGHSSNEKHHKKKPKFQKRIRCQLEVHIS 291
+ L K +NY +SNN S + S ++K K K ++ I Q HI+
Sbjct: 239 VSTFL--KNQQTENYPYENWSNNCPSLDSSISTRSRSKKRRVKTSKNKEEIENQRMTHIA 412
>TC89448 similar to PIR|C84864|C84864 hypothetical protein At2g43280
[imported] - Arabidopsis thaliana, partial (80%)
Length = 1177
Score = 31.6 bits (70), Expect = 0.59
Identities = 19/82 (23%), Positives = 41/82 (49%)
Frame = +2
Query: 145 VIEKDQCAYTLDDEDQKVQTVFMKPEHDKEDTVVLKKTLSCSYPNFSFTEALKKENEKLQ 204
V+ + T+D++D+K+Q + ++ K V ++ L C + +++ ++KL
Sbjct: 611 VVSPREARQTMDEKDKKIQELTVELRIKKRLCVTYQEQLKC------YMNIVEEYSDKLS 772
Query: 205 LELHHSQANMDINQCEFIQRLL 226
+HH N + + E I+ LL
Sbjct: 773 ARIHHILDN--LKEYESIEELL 832
>BG584702 weakly similar to PIR|T04811|T048 STIG1 protein homolog F10M23.220
- Arabidopsis thaliana, partial (43%)
Length = 719
Score = 30.8 bits (68), Expect = 1.0
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +1
Query: 249 VEYSNNSHSDANGSKGHSSNEKHHKKK 275
V SNN+HS+ +G H NE HH K+
Sbjct: 73 VPKSNNNHSNNHGFVPHYHNENHHTKR 153
>BF520164 weakly similar to GP|21593762|gb| unknown {Arabidopsis thaliana},
partial (31%)
Length = 585
Score = 26.2 bits (56), Expect(2) = 2.2
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = +3
Query: 271 HHKKKPKFQKRIRCQLEVHISRKI 294
HH+K+P + R+RC H SR I
Sbjct: 297 HHRKRPFSRPRMRCLPRRHNSRTI 368
Score = 21.9 bits (45), Expect(2) = 2.2
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +2
Query: 297 LIYLLVVLNLIFIFSF 312
L L V LNLI +FSF
Sbjct: 440 LFVLFVELNLILLFSF 487
>BG454439 similar to GP|14517399|gb At2g32810/F24L7.5 {Arabidopsis thaliana},
partial (29%)
Length = 669
Score = 29.6 bits (65), Expect = 2.3
Identities = 14/32 (43%), Positives = 20/32 (61%), Gaps = 2/32 (6%)
Frame = -3
Query: 222 IQRLLDVTNF--AAYSLPEKLSPEDNYQIVEY 251
I R L + +F AA+S K+SP + Y +VEY
Sbjct: 520 ISRTLSIASFG*AAFSFQNKISPSETYILVEY 425
>TC87372 similar to GP|14091469|gb|AAK53758.1 putative potassium transporter
HAK1p {Mesembryanthemum crystallinum}, partial (38%)
Length = 1660
Score = 29.6 bits (65), Expect = 2.3
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = +3
Query: 379 IRGVYGMTSSSIFLKHCHIENVYLSKF 405
I GVY S S+F+ HC N+YL F
Sbjct: 108 IIGVYVSVSYSVFMCHCQCRNLYLLMF 188
>TC76625 similar to SP|O81363|RS4_PRUAR 40S ribosomal protein S4. [Apricot]
{Prunus armeniaca}, partial (13%)
Length = 1172
Score = 29.3 bits (64), Expect = 2.9
Identities = 20/70 (28%), Positives = 32/70 (45%)
Frame = -1
Query: 179 LKKTLSCSYPNFSFTEALKKENEKLQLELHHSQANMDINQCEFIQRLLDVTNFAAYSLPE 238
L KTL+ YP +K +N LQL ++ C+ L D +F + + +
Sbjct: 626 LSKTLTHYYPFAGRLSWIKPQNHLLQLHCNNKGVQFLEATCDIT--LKDFGSFDSTHVVQ 453
Query: 239 KLSPEDNYQI 248
KL P+ NY +
Sbjct: 452 KLVPKINYDV 423
>TC83638 similar to GP|19698943|gb|AAL91207.1 unknown protein {Arabidopsis
thaliana}, partial (16%)
Length = 663
Score = 29.3 bits (64), Expect = 2.9
Identities = 22/81 (27%), Positives = 33/81 (40%), Gaps = 2/81 (2%)
Frame = +2
Query: 213 NMDINQCEFIQRLLD--VTNFAAYSLPEKLSPEDNYQIVEYSNNSHSDANGSKGHSSNEK 270
N + NQ + L D V A P +PE I +Y + D GHS+
Sbjct: 104 NPEANQLPEVDSLPDGLVEGTAEPVAPATPTPEQQKPISDYKEDGFIDC----GHSNELS 271
Query: 271 HHKKKPKFQKRIRCQLEVHIS 291
+ + +FQ C +E HI+
Sbjct: 272 NMPGEKEFQNNHDCSVEAHIT 334
>TC89297 weakly similar to GP|9294431|dbj|BAB02551.1
gene_id:MMB12.13~unknown protein {Arabidopsis thaliana},
partial (56%)
Length = 891
Score = 28.9 bits (63), Expect = 3.8
Identities = 16/42 (38%), Positives = 21/42 (49%), Gaps = 3/42 (7%)
Frame = -3
Query: 216 INQCEFIQRLLDVTNFAAYSL---PEKLSPEDNYQIVEYSNN 254
IN CEFI L N + SL PE + P D ++ Y N+
Sbjct: 604 INDCEFIVVFLMKYNLGSSSLPTKPEMIKPRDTNKLFRYINS 479
>TC83396 similar to GP|22858664|gb|AAN05792.1 unknown {Gossypium hirsutum},
partial (40%)
Length = 921
Score = 28.5 bits (62), Expect = 5.0
Identities = 17/57 (29%), Positives = 30/57 (51%)
Frame = +3
Query: 167 MKPEHDKEDTVVLKKTLSCSYPNFSFTEALKKENEKLQLELHHSQANMDINQCEFIQ 223
+K EH+K+ V L + L ++ TE KKE EKL+ ++ + +D E ++
Sbjct: 90 IKMEHEKK--VELVRKLEMRRADYVKTEKAKKEVEKLESQMMVASQTIDSTSAEIVK 254
>TC88969 similar to GP|17381224|gb|AAL36424.1 unknown protein {Arabidopsis
thaliana}, partial (7%)
Length = 772
Score = 28.1 bits (61), Expect = 6.6
Identities = 15/39 (38%), Positives = 23/39 (58%)
Frame = -1
Query: 353 ILLLLYLLHKEEA*EDDEHSEWREVQIRGVYGMTSSSIF 391
ILL L+K EA + DEH +++ ++GM +SS F
Sbjct: 691 ILLCSIFLYKREATQIDEHIHQELKRLKPLFGMRTSS*F 575
>TC80576 similar to GP|11139266|gb|AAG31651.1 PRLI-interacting factor K
{Arabidopsis thaliana}, partial (41%)
Length = 933
Score = 27.7 bits (60), Expect = 8.6
Identities = 23/70 (32%), Positives = 33/70 (46%), Gaps = 3/70 (4%)
Frame = -3
Query: 296 ILIYLLVVLNLIFIFSFAGIKTFFYPCGTFSKIATVATNRPIFCRSMQ*LY---CIFVGF 352
+ ++LLV L L SF+GI T + S + N F R + L C F+ F
Sbjct: 274 VFLHLLVSLEL----SFSGINT----TRSLSSFNGFSLNTSFFFRFLFLLEFQSCFFLSF 119
Query: 353 ILLLLYLLHK 362
+L LYLL +
Sbjct: 118 LLFFLYLLSR 89
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.139 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,701,322
Number of Sequences: 36976
Number of extensions: 205773
Number of successful extensions: 1531
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1509
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1526
length of query: 417
length of database: 9,014,727
effective HSP length: 99
effective length of query: 318
effective length of database: 5,354,103
effective search space: 1702604754
effective search space used: 1702604754
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC136504.6