BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136472.8 + phase: 0
(182 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86577 PIR|T51183|T51183 actin isoform B [imported] - Mimosa pu... 298 1e-81
TC85697 homologue to SP|P53496|ACTB_ARATH Actin 11. [Mouse-ear c... 296 3e-81
TC86486 PIR|T51179|T51179 actin [imported] - garden pea, complete 293 2e-80
TC89940 homologue to PIR|T51175|T51175 actin [imported] - upland... 293 3e-80
TC85698 homologue to GP|7960247|gb|AAF71265.1| actin-like protei... 156 4e-39
TC93753 homologue to GP|9279623|dbj|BAB01081.1 ARP2 (actin-relat... 141 1e-34
TC91842 similar to GP|21427461|gb|AAM53243.1 actin-related prote... 98 1e-24
TC79646 similar to GP|21593375|gb|AAM65324.1 unknown {Arabidopsi... 103 3e-23
AL369486 similar to GP|12321978|gb actin-like protein; 86009-894... 55 2e-08
BF641907 similar to GP|22136342|gb actin -like protein {Arabidop... 53 6e-08
BE248168 homologue to GP|9279623|dbj ARP2 (actin-related protein... 53 8e-08
BI264438 similar to GP|21427467|gb actin-related protein 6 {Arab... 47 3e-06
TC82569 similar to GP|21593375|gb|AAM65324.1 unknown {Arabidopsi... 43 8e-05
BE204789 similar to PIR|C86318|C86 protein F15H18.8 [imported] -... 30 0.41
TC80175 similar to GP|14582255|gb|AAK69429.1 ZIP-like zinc trans... 29 1.2
TC80719 similar to GP|22136818|gb|AAM91753.1 unknown protein {Ar... 28 2.0
CB894531 homologue to PIR|F84833|F84 probable SNF2/SWI2 family t... 27 5.9
TC84623 similar to GP|9759369|dbj|BAB09828.1 gene_id:MYJ24.10~re... 26 10.0
AW689211 weakly similar to PIR|D86258|D86 protein F5O11.10 [impo... 26 10.0
BQ141768 weakly similar to GP|11036868|gb| PxORF73 peptide {Plut... 26 10.0
>TC86577 PIR|T51183|T51183 actin isoform B [imported] - Mimosa pudica,
complete
Length = 1590
Score = 298 bits (762), Expect = 1e-81
Identities = 144/146 (98%), Positives = 145/146 (98%)
Frame = +3
Query: 1 MADAEDIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEA 60
MADAEDIQPLV DNGTGMVKAGFAGDDAPRAVFPSI+GRPRHTGVMVGMGQKDAYVGDEA
Sbjct: 102 MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 281
Query: 61 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 120
QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK
Sbjct: 282 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 461
Query: 121 MTQIMFETFNVPAMYVAIQAVLSLYA 146
MTQIMFETFNVPAMYVAIQAVLSLYA
Sbjct: 462 MTQIMFETFNVPAMYVAIQAVLSLYA 539
>TC85697 homologue to SP|P53496|ACTB_ARATH Actin 11. [Mouse-ear cress]
{Arabidopsis thaliana}, complete
Length = 1528
Score = 296 bits (758), Expect = 3e-81
Identities = 143/146 (97%), Positives = 144/146 (97%)
Frame = +3
Query: 1 MADAEDIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEA 60
MADAEDIQPLV DNGTGMVKAGFAGDDAPRAVFPSI+GRPRHTGVMVGMGQKDAYVGDEA
Sbjct: 102 MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 281
Query: 61 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 120
QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK
Sbjct: 282 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 461
Query: 121 MTQIMFETFNVPAMYVAIQAVLSLYA 146
MTQIMFETFN PAMYVAIQAVLSLYA
Sbjct: 462 MTQIMFETFNTPAMYVAIQAVLSLYA 539
>TC86486 PIR|T51179|T51179 actin [imported] - garden pea, complete
Length = 1536
Score = 293 bits (751), Expect = 2e-80
Identities = 141/146 (96%), Positives = 144/146 (98%)
Frame = +2
Query: 1 MADAEDIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEA 60
MA++EDIQPLV DNGTGMVKAGFAGDDAPRAVFPSI+GRPRHTGVMVGMGQKDAYVGDEA
Sbjct: 71 MAESEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 250
Query: 61 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 120
QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK
Sbjct: 251 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 430
Query: 121 MTQIMFETFNVPAMYVAIQAVLSLYA 146
MTQIMFETFN PAMYVAIQAVLSLYA
Sbjct: 431 MTQIMFETFNTPAMYVAIQAVLSLYA 508
>TC89940 homologue to PIR|T51175|T51175 actin [imported] - upland cotton,
complete
Length = 1429
Score = 293 bits (749), Expect = 3e-80
Identities = 142/146 (97%), Positives = 143/146 (97%)
Frame = +1
Query: 1 MADAEDIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEA 60
MADAEDI PLV DNGTGMVKAGFAGDDAPRAVFPSI+GRPRHTGVMVGMGQKDAYVGDEA
Sbjct: 49 MADAEDI*PLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 228
Query: 61 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 120
QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK
Sbjct: 229 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 408
Query: 121 MTQIMFETFNVPAMYVAIQAVLSLYA 146
MTQIMFETFN PAMYVAIQAVLSLYA
Sbjct: 409 MTQIMFETFNAPAMYVAIQAVLSLYA 486
>TC85698 homologue to GP|7960247|gb|AAF71265.1| actin-like protein
{Phalaenopsis sp. 'True Lady'}, partial (80%)
Length = 1459
Score = 156 bits (395), Expect = 4e-39
Identities = 74/74 (100%), Positives = 74/74 (100%)
Frame = +1
Query: 73 IEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVP 132
IEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVP
Sbjct: 1 IEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVP 180
Query: 133 AMYVAIQAVLSLYA 146
AMYVAIQAVLSLYA
Sbjct: 181 AMYVAIQAVLSLYA 222
>TC93753 homologue to GP|9279623|dbj|BAB01081.1 ARP2 (actin-related protein
2) {Arabidopsis thaliana}, partial (49%)
Length = 688
Score = 141 bits (356), Expect = 1e-34
Identities = 66/139 (47%), Positives = 91/139 (64%), Gaps = 2/139 (1%)
Frame = +1
Query: 10 LVVDNGTGMVKAGFAGDDAPRAVFPSIIGRP--RHTGVMVGMGQKDAYVGDEAQSKRGIL 67
+V DNGTG VK GFAG++ P +VFP ++GRP R+ + KD VG+ R L
Sbjct: 124 VVCDNGTGYVKCGFAGENFPTSVFPCVVGRPMLRYEESLTEQELKDIVVGEGCADLRHQL 303
Query: 68 TLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 127
+ YP+ +GIV NWDDM +W H FYNEL++ P++ +LLT+ PLNP NREKM + M E
Sbjct: 304 DISYPVNNGIVQNWDDMCHVWDHAFYNELKINPQDCKILLTDPPLNPSKNREKMVETMLE 483
Query: 128 TFNVPAMYVAIQAVLSLYA 146
+N +++ IQA L+LYA
Sbjct: 484 KYNFAGVFIQIQAGLTLYA 540
>TC91842 similar to GP|21427461|gb|AAM53243.1 actin-related protein 3
{Arabidopsis thaliana}, partial (78%)
Length = 1021
Score = 98.2 bits (243), Expect(2) = 1e-24
Identities = 48/101 (47%), Positives = 63/101 (61%), Gaps = 3/101 (2%)
Frame = +3
Query: 49 MGQKDAYVGDEAQSKR---GILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPV 105
M D ++GD+A SK L YPI+HG V NWD ME+ W +N LR PE+H
Sbjct: 168 MADLDFFIGDDALSKSRSSSTYNLTYPIKHGQVENWDAMERFWQXCIFNYLRCDPEDHYF 347
Query: 106 LLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYA 146
LLT +PL +RE +IMFETFNVP +Y+A+ +VL+L A
Sbjct: 348 LLTXSPLTAPESREYTGEIMFETFNVPGLYIAVNSVLALAA 470
Score = 30.8 bits (68), Expect(2) = 1e-24
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +2
Query: 11 VVDNGTGMVKAGFAGDDAPRAVFPSII 37
V+ NG+G K GFAG+ P + PS++
Sbjct: 8 VIXNGSGYTKMGFAGNVEPCFIVPSVV 88
>TC79646 similar to GP|21593375|gb|AAM65324.1 unknown {Arabidopsis
thaliana}, partial (38%)
Length = 706
Score = 103 bits (258), Expect = 3e-23
Identities = 56/172 (32%), Positives = 93/172 (53%), Gaps = 26/172 (15%)
Frame = +1
Query: 1 MADAEDIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIG---------------------- 38
M +++ +V+D G+ KAG+AG+DAP+AVFPS++G
Sbjct: 97 MYGGDEVSAIVIDLGSHTCKAGYAGEDAPKAVFPSVVGAIDQMDVDESDNAEKNSGSGES 276
Query: 39 --RPRHTGVMVGMGQKDAYVGDEAQS-KRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNE 95
R+ G++ YVG ++ +R + + P++ GIV++WD ++ IW H F E
Sbjct: 277 KNNIRNVDGDKAKGKRKLYVGSQSLGYRRDFMEVVSPLKDGIVADWDIVDSIWDHAFSRE 456
Query: 96 -LRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYA 146
L + P+EHP+LL E N + RE+ ++MFE + PA++ A AVL+ +A
Sbjct: 457 CLLIDPKEHPMLLAEPSSNAQQQRERAAELMFEKYKAPALFXAKNAVLTSFA 612
>AL369486 similar to GP|12321978|gb actin-like protein; 86009-89485
{Arabidopsis thaliana}, partial (18%)
Length = 427
Score = 54.7 bits (130), Expect = 2e-08
Identities = 40/119 (33%), Positives = 55/119 (45%), Gaps = 6/119 (5%)
Frame = +3
Query: 9 PLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDE--AQSKRGI 66
P+V+ NG + G+AG+ PR VF +I+ RPRH G+ VGD A K
Sbjct: 78 PIVIHNGASYFRIGWAGESEPRVVFRNIVQRPRHKAT----GETVTIVGDHDPALLKYFD 245
Query: 67 LTLKYP---IEHGIVSNWDDMEKIWHHTFYNELRVAPE-EHPVLLTEAPLNPKANREKM 121
T P E +V ++ ME I F +HP+L+TE NP +R KM
Sbjct: 246 CTRSGPRSAFESDVVFQFEIMEYILDFAFDRMGATGSGIDHPILITECVCNPVQSRSKM 422
>BF641907 similar to GP|22136342|gb actin -like protein {Arabidopsis
thaliana}, partial (53%)
Length = 621
Score = 53.1 bits (126), Expect = 6e-08
Identities = 24/45 (53%), Positives = 32/45 (70%)
Frame = +2
Query: 102 EHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYA 146
E +L T+ PKAN+E++ Q+MFETFN+ Y + QAVLSLYA
Sbjct: 56 EGQILFTDPLCTPKANKEQLVQLMFETFNISGFYASEQAVLSLYA 190
>BE248168 homologue to GP|9279623|dbj ARP2 (actin-related protein 2)
{Arabidopsis thaliana}, partial (26%)
Length = 329
Score = 52.8 bits (125), Expect = 8e-08
Identities = 23/40 (57%), Positives = 30/40 (74%)
Frame = +1
Query: 107 LTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYA 146
LT+ PLNP NREKM + MFE +N +++ IQAVL+LYA
Sbjct: 1 LTDPPLNPSKNREKMVETMFEKYNFAGVFIQIQAVLTLYA 120
>BI264438 similar to GP|21427467|gb actin-related protein 6 {Arabidopsis
thaliana}, partial (43%)
Length = 695
Score = 47.4 bits (111), Expect = 3e-06
Identities = 22/70 (31%), Positives = 40/70 (56%)
Frame = +3
Query: 68 TLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 127
+++ PI+ G + N D IW H F + LR+ P + +LLT+ + + + Q++FE
Sbjct: 78 SVRRPIDRGYLINPDLQRDIWSHLFTSILRINPSQSSLLLTQPLFTLPSIQHSLHQLVFE 257
Query: 128 TFNVPAMYVA 137
FN +++VA
Sbjct: 258 DFNFSSLFVA 287
>TC82569 similar to GP|21593375|gb|AAM65324.1 unknown {Arabidopsis
thaliana}, partial (13%)
Length = 687
Score = 42.7 bits (99), Expect = 8e-05
Identities = 31/103 (30%), Positives = 50/103 (48%), Gaps = 13/103 (12%)
Frame = +3
Query: 7 IQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEAQ----- 61
+ +V+D G+ KA +AG+DAP+AVFPS++G + Q D D+A+
Sbjct: 216 VSAIVIDLGSHTCKAVYAGEDAPKAVFPSVVG---------AIDQMDVDESDDAEKTLAP 368
Query: 62 -SKRGILTLKYPIEHG-------IVSNWDDMEKIWHHTFYNEL 96
++R IL + I+ +S W +E +W F N L
Sbjct: 369 GNQRTILEMLMGIKPRERENCM*DLSPWATVEILWR*PFCNVL 497
Score = 26.6 bits (57), Expect = 5.9
Identities = 9/21 (42%), Positives = 15/21 (70%)
Frame = +3
Query: 72 PIEHGIVSNWDDMEKIWHHTF 92
P++ GIV++WD ++ I H F
Sbjct: 585 PLKDGIVADWDIVDSIXDHAF 647
>BE204789 similar to PIR|C86318|C86 protein F15H18.8 [imported] - Arabidopsis
thaliana, partial (14%)
Length = 567
Score = 30.4 bits (67), Expect = 0.41
Identities = 18/71 (25%), Positives = 31/71 (43%)
Frame = +1
Query: 76 GIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMY 135
GIV++WD ++ I H F + ++MFE + PA++
Sbjct: 1 GIVADWDIVDSI*DHAF---------------------------RAAELMFEKYKAPALF 99
Query: 136 VAIQAVLSLYA 146
+A AVL+ +A
Sbjct: 100 LAKNAVLTSFA 132
>TC80175 similar to GP|14582255|gb|AAK69429.1 ZIP-like zinc transporter
{Thlaspi caerulescens}, partial (69%)
Length = 1223
Score = 28.9 bits (63), Expect = 1.2
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -3
Query: 71 YPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEH 103
YP H + N +D + + HH F + VA EH
Sbjct: 639 YPHHHALQMNENDHDHVHHHGFDVNVHVATHEH 541
>TC80719 similar to GP|22136818|gb|AAM91753.1 unknown protein {Arabidopsis
thaliana}, partial (41%)
Length = 1280
Score = 28.1 bits (61), Expect = 2.0
Identities = 12/28 (42%), Positives = 16/28 (56%)
Frame = -2
Query: 87 IWHHTFYNELRVAPEEHPVLLTEAPLNP 114
+WH FY +LR+ P HP+ PL P
Sbjct: 1039 LWH--FYEDLRLMPMHHPLQRQLNPLQP 962
>CB894531 homologue to PIR|F84833|F84 probable SNF2/SWI2 family transcription
factor [imported] - Arabidopsis thaliana, partial (2%)
Length = 267
Score = 26.6 bits (57), Expect = 5.9
Identities = 22/87 (25%), Positives = 35/87 (39%)
Frame = +2
Query: 38 GRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELR 97
GR HT + G ++ G E + I L I+HG N L
Sbjct: 50 GRKAHTAISQFRGIQNGTKGCEGEEPISIQVLLLLIQHGA----------------NGLN 181
Query: 98 VAPEEHPVLLTEAPLNPKANREKMTQI 124
+ +H V+L E LNP A + ++++
Sbjct: 182 LLEAQH-VVLVEPLLNPAAEAQAISRV 259
>TC84623 similar to GP|9759369|dbj|BAB09828.1
gene_id:MYJ24.10~ref|NP_055178.1~similar to unknown
protein {Arabidopsis thaliana}, partial (3%)
Length = 634
Score = 25.8 bits (55), Expect = 10.0
Identities = 16/52 (30%), Positives = 22/52 (41%)
Frame = -2
Query: 51 QKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEE 102
+K + +AQ +T + HG VS D EK W H + AP E
Sbjct: 366 RKQHHYKPKAQVLATAITNRTATTHGRVSGGQDREKRW*HRHHRRSE*AP*E 211
>AW689211 weakly similar to PIR|D86258|D86 protein F5O11.10 [imported] -
Arabidopsis thaliana, partial (12%)
Length = 366
Score = 25.8 bits (55), Expect = 10.0
Identities = 12/36 (33%), Positives = 18/36 (49%)
Frame = -2
Query: 82 DDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKAN 117
+ +EK TFY +L + H + T +NPK N
Sbjct: 227 NQLEKEKLETFYAQLLTKKQNHNIHKTNQQVNPKQN 120
>BQ141768 weakly similar to GP|11036868|gb| PxORF73 peptide {Plutella
xylostella granulovirus}, partial (29%)
Length = 958
Score = 25.8 bits (55), Expect = 10.0
Identities = 17/55 (30%), Positives = 23/55 (40%)
Frame = +1
Query: 60 AQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNP 114
A S G L P+ HG + N H LRV+P HP + +P+ P
Sbjct: 31 APSSGGPLPPTPPLRHGPLFNKPRPPSPSH------LRVSPPSHPFFIPSSPIPP 177
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.136 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,753,500
Number of Sequences: 36976
Number of extensions: 43413
Number of successful extensions: 239
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 233
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 236
length of query: 182
length of database: 9,014,727
effective HSP length: 90
effective length of query: 92
effective length of database: 5,686,887
effective search space: 523193604
effective search space used: 523193604
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC136472.8