BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136287.2 + phase: 0
(365 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83530 similar to GP|11994783|dbj|BAB03173. gene_id:T19N8.5~pir... 337 e-130
TC89481 similar to GP|13605619|gb|AAK32803.1 AT3g49260/F2K15_120... 33 0.13
TC79072 30 1.5
TC84389 weakly similar to GP|10177368|dbj|BAB10659. contains sim... 28 4.3
TC83850 weakly similar to GP|7300071|gb|AAF55241.1| CG4225 gene ... 28 5.6
TC81205 similar to GP|20161237|dbj|BAB90164. putative receptor-l... 28 5.6
CB891912 similar to GP|2739382|gb| unknown protein {Arabidopsis ... 28 5.6
TC92042 similar to PIR|T49263|T49263 hypothetical protein F12M12... 28 7.3
TC84716 homologue to GP|17064160|gb|AAL35287.1 WRKY transcriptio... 28 7.3
TC91131 weakly similar to GP|8843864|dbj|BAA97390.1 contains sim... 28 7.3
AW695045 27 9.5
TC89506 similar to GP|21537298|gb|AAM61639.1 unknown {Arabidopsi... 27 9.5
>TC83530 similar to GP|11994783|dbj|BAB03173.
gene_id:T19N8.5~pir||H69176~similar to unknown protein
{Arabidopsis thaliana}, partial (52%)
Length = 816
Score = 337 bits (863), Expect(2) = e-130
Identities = 175/190 (92%), Positives = 178/190 (93%), Gaps = 2/190 (1%)
Frame = +3
Query: 68 KEILDAQNKKVATIFDVSTPEELERLRPEDEQAENIVVNLLDWQVIPAENIIAAFQNSQK 127
+ ILDAQNKKVATIFDVSTPEELERLRPEDEQAENIVVNLLDWQVIPAENIIAAFQNSQK
Sbjct: 246 RSILDAQNKKVATIFDVSTPEELERLRPEDEQAENIVVNLLDWQVIPAENIIAAFQNSQK 425
Query: 128 TVFAISDNASEAQTFLEALEHGLDGIVLKVEDVEPMLELKEYFDRRTEESNVLNLTKATV 187
TVFAISDNASEAQTFLEALEHGLDGIVLKVEDVEPMLELKEYFDRRTEESNVLNLTKATV
Sbjct: 426 TVFAISDNASEAQTFLEALEHGLDGIVLKVEDVEPMLELKEYFDRRTEESNVLNLTKATV 605
Query: 188 TQIQVAGMGDRVCVDLCSLMRPGEGLLVGSFARGLFLVHSECLESKLHCKQTF--SGECV 245
TQIQVAGMGDRVCVDLCSLMRPGEGLLVGSFARGLFLVHSECLES + F + V
Sbjct: 606 TQIQVAGMGDRVCVDLCSLMRPGEGLLVGSFARGLFLVHSECLESNYIASRPFRVNAGPV 785
Query: 246 HAYIAVPGGR 255
HAYIAVPGGR
Sbjct: 786 HAYIAVPGGR 815
Score = 146 bits (369), Expect(2) = e-130
Identities = 68/69 (98%), Positives = 68/69 (98%)
Frame = +2
Query: 1 MSSSSWTPLEDSGNKKSKKVWIWTKNKQVMTAAVERGWNTFIFPSNLPHLANDWSSIAVI 60
MSSSSWTP EDSGNKKSKKVWIWTKNKQVMTAAVERGWNTFIFPSNLPHLANDWSSIAVI
Sbjct: 44 MSSSSWTPSEDSGNKKSKKVWIWTKNKQVMTAAVERGWNTFIFPSNLPHLANDWSSIAVI 223
Query: 61 CPLFASEKE 69
CPLFASEKE
Sbjct: 224 CPLFASEKE 250
>TC89481 similar to GP|13605619|gb|AAK32803.1 AT3g49260/F2K15_120
{Arabidopsis thaliana}, partial (25%)
Length = 828
Score = 33.5 bits (75), Expect = 0.13
Identities = 26/114 (22%), Positives = 47/114 (40%), Gaps = 1/114 (0%)
Frame = +2
Query: 64 FASEKEILDAQNKKVATIFDVSTPEELERLRPEDEQAENIVVNLLDWQVIPAENIIAAFQ 123
F+S K++ + + T VSTP + E+ + E+ E+ V + ++ PAE+
Sbjct: 176 FSSVKKVFKSSSSSKDTSVPVSTPVQPEKKKENKEKLEHEVAEEVSFEHFPAESSPDDVT 355
Query: 124 NSQKTVFA-ISDNASEAQTFLEALEHGLDGIVLKVEDVEPMLELKEYFDRRTEE 176
N T + D+ + A EA V+ + ++ L Y EE
Sbjct: 356 NEGSTTSTPVRDDRNHAIAVAEATAAAASAAVVAAQAAARVVRLAGYGRHNKEE 517
>TC79072
Length = 2401
Score = 30.0 bits (66), Expect = 1.5
Identities = 11/46 (23%), Positives = 24/46 (51%)
Frame = +2
Query: 15 KKSKKVWIWTKNKQVMTAAVERGWNTFIFPSNLPHLANDWSSIAVI 60
K++ ++ W +N + AVE GW+ F F S ++ + + ++
Sbjct: 323 KRTTNLFSWIENPSMAADAVENGWSRFAFTSYKSYMPSPSKKVTLL 460
>TC84389 weakly similar to GP|10177368|dbj|BAB10659. contains similarity to
heat shock transcription factor HSF30~gene_id:K16L22.13,
partial (8%)
Length = 692
Score = 28.5 bits (62), Expect = 4.3
Identities = 12/44 (27%), Positives = 26/44 (58%)
Frame = +1
Query: 41 FIFPSNLPHLANDWSSIAVICPLFASEKEILDAQNKKVATIFDV 84
F+F ++ W+ +A++CPLF S + +L ++ +T+ +V
Sbjct: 385 FLFFTSKTKPFKTWNPLAILCPLFFSYEILLYPLDRSTSTVQNV 516
>TC83850 weakly similar to GP|7300071|gb|AAF55241.1| CG4225 gene product
{Drosophila melanogaster}, partial (23%)
Length = 816
Score = 28.1 bits (61), Expect = 5.6
Identities = 21/85 (24%), Positives = 39/85 (45%)
Frame = +2
Query: 261 ELKSGKEVIVVDQQGRQRIAIVGRVKIESRPLILVEAKTDSDNQTISILLQNAETVALVC 320
E K G+ + + RQRIAI + ++R L+L EA D+ T + E V
Sbjct: 359 ETKVGERGLKLSGGERQRIAIARAILKDARILLLDEATASLDSHTERQIQDALERV---- 526
Query: 321 PVQGNKLLKTAFPVTSLKVGDEVLL 345
G + A ++++ D++++
Sbjct: 527 -TAGRTTITIAHRLSTITTSDQIVV 598
>TC81205 similar to GP|20161237|dbj|BAB90164. putative receptor-like protein
kinase {Oryza sativa (japonica cultivar-group)}, partial
(12%)
Length = 961
Score = 28.1 bits (61), Expect = 5.6
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +2
Query: 219 ARGLFLVHSECLESKLHC 236
A+GL +H ECLE LHC
Sbjct: 119 AKGLAYLHEECLEWVLHC 172
>CB891912 similar to GP|2739382|gb| unknown protein {Arabidopsis thaliana},
partial (22%)
Length = 589
Score = 28.1 bits (61), Expect = 5.6
Identities = 19/70 (27%), Positives = 37/70 (52%)
Frame = +2
Query: 71 LDAQNKKVATIFDVSTPEELERLRPEDEQAENIVVNLLDWQVIPAENIIAAFQNSQKTVF 130
L A+ A + + EEL++++ E EQA+ V+ L+ +++ A+ I A + S+K
Sbjct: 146 LTAEEANQANLLAQAAREELQKVKAEAEQAK-AGVSTLESRLLAAQKEIEAAKASEKLAI 322
Query: 131 AISDNASEAQ 140
A E++
Sbjct: 323 AAIKALQESE 352
>TC92042 similar to PIR|T49263|T49263 hypothetical protein F12M12.190 -
Arabidopsis thaliana, partial (29%)
Length = 992
Score = 27.7 bits (60), Expect = 7.3
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = +1
Query: 118 IIAAFQNSQKTVFAISDNASEAQTFLEALEHGLDGIVLKVEDVEPMLELKE 168
II+ + +K +F DNA + L++L+ LD L ++ E LEL E
Sbjct: 34 IISTWMEKKKALF--KDNAERMKQLLDSLQKKLDESFLNMQLYEKALELFE 180
>TC84716 homologue to GP|17064160|gb|AAL35287.1 WRKY transcription factor 29
{Arabidopsis thaliana}, partial (21%)
Length = 524
Score = 27.7 bits (60), Expect = 7.3
Identities = 20/77 (25%), Positives = 35/77 (44%), Gaps = 3/77 (3%)
Frame = +3
Query: 119 IAAFQNSQKTVFAISDNASEAQTFLEALEH---GLDGIVLKVEDVEPMLELKEYFDRRTE 175
+ FQNSQK + ++ N + L H L + L ++ + +LK+ + +
Sbjct: 84 LEVFQNSQKLLMSLFMNLKSFVNLISILFHLKPSLQVL*LPLKSLNKSKKLKQLLIKICK 263
Query: 176 ESNVLNLTKATVTQIQV 192
LNL KA T+ +V
Sbjct: 264 FQQYLNLKKARKTRTRV 314
>TC91131 weakly similar to GP|8843864|dbj|BAA97390.1 contains similarity to
receptor-like protein kinase~gene_id:MWD22.22
{Arabidopsis thaliana}, partial (11%)
Length = 1413
Score = 27.7 bits (60), Expect = 7.3
Identities = 16/28 (57%), Positives = 16/28 (57%)
Frame = +2
Query: 2 SSSSWTPLEDSGNKKSKKVWIWTKNKQV 29
SSSS L GNKKSK V W NK V
Sbjct: 224 SSSSVVALAIKGNKKSKYVVQWALNKFV 307
>AW695045
Length = 525
Score = 27.3 bits (59), Expect = 9.5
Identities = 11/39 (28%), Positives = 19/39 (48%)
Frame = -2
Query: 11 DSGNKKSKKVWIWTKNKQVMTAAVERGWNTFIFPSNLPH 49
D + K K+ W W + + +E W++F+ P PH
Sbjct: 122 DRIDMKMKR*WYWWCSNPQTSLELE*QWHSFLLPRFFPH 6
>TC89506 similar to GP|21537298|gb|AAM61639.1 unknown {Arabidopsis
thaliana}, partial (52%)
Length = 796
Score = 27.3 bits (59), Expect = 9.5
Identities = 11/39 (28%), Positives = 19/39 (48%)
Frame = -3
Query: 11 DSGNKKSKKVWIWTKNKQVMTAAVERGWNTFIFPSNLPH 49
D + K K+ W W + + +E W++F+ P PH
Sbjct: 122 DRIDMKMKR*WYWWCSNPQTSLELE*QWHSFLLPRFFPH 6
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.133 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,904,907
Number of Sequences: 36976
Number of extensions: 124097
Number of successful extensions: 615
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 611
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 614
length of query: 365
length of database: 9,014,727
effective HSP length: 97
effective length of query: 268
effective length of database: 5,428,055
effective search space: 1454718740
effective search space used: 1454718740
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC136287.2