BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136286.14 - phase: 0
(159 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC90880 similar to GP|15027941|gb|AAK76501.1 unknown protein {Ar... 303 3e-83
TC76431 similar to GP|8132441|gb|AAF73291.1| extensin {Pisum sat... 39 0.001
TC80732 similar to GP|21553690|gb|AAM62783.1 unknown {Arabidopsi... 33 0.049
TC78356 weakly similar to GP|18092161|gb|AAL59140.1 cGMP-gated c... 32 0.083
AW695267 similar to GP|15450731|gb At2g40130/T7M7.2 {Arabidopsis... 32 0.11
TC86669 similar to SP|P82244|RK34_SPIOL 50S ribosomal protein L3... 32 0.14
TC79871 similar to GP|19310486|gb|AAL84977.1 AT5g13970/MAC12_6 {... 32 0.14
BQ141909 weakly similar to GP|14253159|emb VMP3 protein {Volvox ... 31 0.19
TC93063 similar to PIR|T08852|T08852 lustrin A - California red ... 31 0.19
TC86789 similar to PIR|A84594|A84594 hypothetical protein At2g20... 31 0.19
TC77641 similar to GP|21595125|gb|AAM66074.1 endosperm-specific ... 31 0.24
TC79671 similar to GP|21537305|gb|AAM61646.1 unknown {Arabidopsi... 31 0.24
BE204494 homologue to GP|10179298|dbj cytochrome oxydase subunit... 30 0.32
TC89313 homologue to PIR|T48412|T48412 RIBOSOMAL PROTEIN S28-lik... 30 0.32
TC83965 weakly similar to GP|21592320|gb|AAM64271.1 unknown {Ara... 30 0.32
BI271192 30 0.32
BE324536 similar to GP|7299263|gb| CG9461 gene product {Drosophi... 30 0.32
TC82543 similar to GP|9294225|dbj|BAB02127.1 gene_id:K17E12.14~u... 30 0.41
BF647189 similar to GP|8885590|dbj| gene_id:F24B18.9~pir||T05755... 30 0.54
TC77478 similar to GP|9884651|dbj|BAB11979.1 MGDG synthase type ... 30 0.54
>TC90880 similar to GP|15027941|gb|AAK76501.1 unknown protein {Arabidopsis
thaliana}, partial (36%)
Length = 1051
Score = 303 bits (775), Expect = 3e-83
Identities = 150/150 (100%), Positives = 150/150 (100%)
Frame = +3
Query: 10 TLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEP 69
TLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEP
Sbjct: 3 TLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEP 182
Query: 70 KPELNESELASELKKAMQERKEQEGNNFWNGVVSEIGEIEWPEFGKVLGTTGVVLSVIFG 129
KPELNESELASELKKAMQERKEQEGNNFWNGVVSEIGEIEWPEFGKVLGTTGVVLSVIFG
Sbjct: 183 KPELNESELASELKKAMQERKEQEGNNFWNGVVSEIGEIEWPEFGKVLGTTGVVLSVIFG 362
Query: 130 SSAVLLTLNAILAELSDKVFAGKGVQDFFT 159
SSAVLLTLNAILAELSDKVFAGKGVQDFFT
Sbjct: 363 SSAVLLTLNAILAELSDKVFAGKGVQDFFT 452
>TC76431 similar to GP|8132441|gb|AAF73291.1| extensin {Pisum sativum},
partial (30%)
Length = 890
Score = 38.5 bits (88), Expect = 0.001
Identities = 24/62 (38%), Positives = 31/62 (49%)
Frame = +3
Query: 12 TLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKP 71
++SP TP PPST +P P LPS LT T + ++ T+NP P P P P
Sbjct: 30 SISPLSTPPPPSTPTP--------PPLPSSPLTSPIT-*KTIQVSISTTTNPPPLPSPPP 182
Query: 72 EL 73
L
Sbjct: 183SL 188
>TC80732 similar to GP|21553690|gb|AAM62783.1 unknown {Arabidopsis
thaliana}, partial (16%)
Length = 760
Score = 33.1 bits (74), Expect = 0.049
Identities = 27/105 (25%), Positives = 47/105 (44%), Gaps = 13/105 (12%)
Frame = +2
Query: 5 AKTSFTLTLSPHFTPSPPSTLSPQFFNFKLKPSLP---------SLTLTRRCTLPRAVEE 55
+ +SF++ + HF + ST+ KL P P S ++ P++
Sbjct: 11 SSSSFSIGFASHFLHA--STVFASLIRSKLLPPKP*HFKMSEPESQNESQPSPEPQSQNG 184
Query: 56 KDQPTSN----PEPEPEPKPELNESELASELKKAMQERKEQEGNN 96
+P SN P+P+PE +PE ES+ +L+ R + E N+
Sbjct: 185 APEPESNSNHEPDPQPETEPEQPESQEQVQLEPESGSRPDPEVNS 319
>TC78356 weakly similar to GP|18092161|gb|AAL59140.1 cGMP-gated channel
{Oncorhynchus mykiss}, partial (4%)
Length = 854
Score = 32.3 bits (72), Expect = 0.083
Identities = 26/88 (29%), Positives = 36/88 (40%), Gaps = 1/88 (1%)
Frame = +1
Query: 29 FFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELNESELASELKKAMQE 88
FFNF + S+ C E +P S PEP P P + E+K E
Sbjct: 226 FFNFLIFFQY*SIFNMGNCGSNPKTNEGPEPVSVPEPAPVP--------ITEEVKVGATE 381
Query: 89 RKEQEGNNFWNGVVSE-IGEIEWPEFGK 115
E N N +++E + E + PE GK
Sbjct: 382 ETPVEDNKSLNTLLNENVEEAKKPEEGK 465
>AW695267 similar to GP|15450731|gb At2g40130/T7M7.2 {Arabidopsis
thaliana}, partial (29%)
Length = 625
Score = 32.0 bits (71), Expect = 0.11
Identities = 19/54 (35%), Positives = 28/54 (51%), Gaps = 3/54 (5%)
Frame = +2
Query: 4 QAKTSFTLTLSPHFTPSPPSTLS---PQFFNFKLKPSLPSLTLTRRCTLPRAVE 54
Q KT+F L PH PS S L P F+ +KL+ ++ R+C P A++
Sbjct: 20 QHKTTF-LIYKPHLLPSLISKLFSVIPSFYEYKLQTMPTPVSSARQCLTPEAIQ 178
>TC86669 similar to SP|P82244|RK34_SPIOL 50S ribosomal protein L34
chloroplast precursor. [Spinach] {Spinacia oleracea},
partial (51%)
Length = 705
Score = 31.6 bits (70), Expect = 0.14
Identities = 20/54 (37%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = -2
Query: 33 KLKPSLPSLTLTRR--CTLPRAVEEKDQPTSNPEPEPEPKPELNESELASELKK 84
K + LP+ T+TRR C L + K SNP +PE + EL E E+ E+ +
Sbjct: 305 KQRACLPAATITRRPFCLLGPTLGFK----SNPSDKPEMEEELKEREVGEEVNE 156
>TC79871 similar to GP|19310486|gb|AAL84977.1 AT5g13970/MAC12_6 {Arabidopsis
thaliana}, partial (6%)
Length = 1596
Score = 31.6 bits (70), Expect = 0.14
Identities = 26/93 (27%), Positives = 39/93 (40%), Gaps = 7/93 (7%)
Frame = +1
Query: 8 SFTLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEE--KDQPTSNPEP 65
SF L L S + F + + + SL + +L EE K +P P+P
Sbjct: 40 SFFLILERANQKSRIESTMEDSFRVRAERAFGSLPIPSSASLWSLSEEEIKPEPKPKPKP 219
Query: 66 EPEPKPELNESELAS-----ELKKAMQERKEQE 93
+ EPKP S +S ELK + + +E E
Sbjct: 220 DSEPKPYPTSSSSSSSGFKVELKNELDDEEEDE 318
>BQ141909 weakly similar to GP|14253159|emb VMP3 protein {Volvox carteri f.
nagariensis}, partial (13%)
Length = 1223
Score = 31.2 bits (69), Expect = 0.19
Identities = 23/69 (33%), Positives = 27/69 (38%), Gaps = 3/69 (4%)
Frame = -2
Query: 8 SFTLTLSPHFTPSPPSTLSPQFFNFKLKP--SLPSLTLTRR-CTLPRAVEEKDQPTSNPE 64
+F SP P PP P + P S PSLTL C+ P + P S P
Sbjct: 436 AFLSPFSPSTFPPPPPCPIPSLYFSLAAPLRSSPSLTLPPSFCSSPPFLPPPPPPPSPPP 257
Query: 65 PEPEPKPEL 73
P P P L
Sbjct: 256 PPPPPSSPL 230
>TC93063 similar to PIR|T08852|T08852 lustrin A - California red abalone,
partial (4%)
Length = 1058
Score = 31.2 bits (69), Expect = 0.19
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = -1
Query: 54 EEKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQE 93
E + +P + PEPE EP+PE A +A E +++E
Sbjct: 617 EPEPEPEAEPEPEAEPEPEAEPEPEAEPEPEAEPEPEDEE 498
Score = 30.4 bits (67), Expect = 0.32
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = -1
Query: 54 EEKDQPTSNPEPEPEPKPELNESE 77
E + +P + PEPE EP+PE E E
Sbjct: 563 EAEPEPEAEPEPEAEPEPEDEEEE 492
Score = 30.0 bits (66), Expect = 0.41
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = -1
Query: 54 EEKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQE 93
E D+P PEPEPE +PE E+E E + + E E
Sbjct: 641 EADDEPEPEPEPEPEAEPE-PEAEPEPEAEPEPEAEPEPE 525
Score = 29.6 bits (65), Expect = 0.54
Identities = 17/40 (42%), Positives = 24/40 (59%)
Frame = -1
Query: 54 EEKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQE 93
E + +P + PEPE EP+PE E E +E + E +EQE
Sbjct: 599 EAEPEPEAEPEPEAEPEPEA-EPEPEAE-PEPEDEEEEQE 486
Score = 27.7 bits (60), Expect = 2.1
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = -1
Query: 52 AVEEKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQE 93
A E++ ++ EPEPEP+PE E+E E + + E E
Sbjct: 665 AAEDEADAEADDEPEPEPEPE-PEAEPEPEAEPEPEAEPEPE 543
>TC86789 similar to PIR|A84594|A84594 hypothetical protein At2g20840
[imported] - Arabidopsis thaliana, partial (93%)
Length = 1421
Score = 31.2 bits (69), Expect = 0.19
Identities = 33/106 (31%), Positives = 42/106 (39%)
Frame = +3
Query: 12 TLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKP 71
T +P PS S LSP LP R T+ D P + + +
Sbjct: 288 TTNPGSVPSAASRLSP----------LPHEPYDRNATV-------DIPLDSSKDVKAKEK 416
Query: 72 ELNESELASELKKAMQERKEQEGNNFWNGVVSEIGEIEWPEFGKVL 117
EL E SELKK QE K +E G+V E E WP F ++
Sbjct: 417 ELQARE--SELKKREQELKRREDAIARAGIVIE--EKNWPPFFPII 542
>TC77641 similar to GP|21595125|gb|AAM66074.1 endosperm-specific
protein-like protein {Arabidopsis thaliana}, partial
(82%)
Length = 1488
Score = 30.8 bits (68), Expect = 0.24
Identities = 24/75 (32%), Positives = 30/75 (40%), Gaps = 1/75 (1%)
Frame = +3
Query: 14 SPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSN-PEPEPEPKPE 72
+PH PSPP TLSP K S PS + + T P S P +P P
Sbjct: 210 TPHSLPSPPPTLSP-----SSKKSSPS*SSSTTSTTLNFTNSPMAPLSPLPSSKPPATPS 374
Query: 73 LNESELASELKKAMQ 87
L S KA++
Sbjct: 375 TTSDPLTSPT*KAVK 419
>TC79671 similar to GP|21537305|gb|AAM61646.1 unknown {Arabidopsis
thaliana}, partial (36%)
Length = 947
Score = 30.8 bits (68), Expect = 0.24
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = +1
Query: 55 EKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQEGNNF 97
E P PEPEPEPKP+ E +L + + A+ + +GN F
Sbjct: 76 ENYTPPPPPEPEPEPKPQFTE-DLVNLREDAV--TADDQGNRF 195
>BE204494 homologue to GP|10179298|dbj cytochrome oxydase subunit 1 {Euhadra
quaesita}, partial (7%)
Length = 573
Score = 30.4 bits (67), Expect = 0.32
Identities = 26/79 (32%), Positives = 35/79 (43%), Gaps = 4/79 (5%)
Frame = -3
Query: 8 SFTLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEP 67
SF L +S + PST +FF KL L R TLP + P S +P P
Sbjct: 502 SFILFISSSLS*LLPSTEFSRFF*SKLNSIHFINLLLRSLTLPGLLVHHSYPKSLSKPSP 323
Query: 68 EPKPE----LNESELASEL 82
+ PE L+ + ASE+
Sbjct: 322 KGGPESTSWLSATPFASEI 266
>TC89313 homologue to PIR|T48412|T48412 RIBOSOMAL PROTEIN S28-like -
Arabidopsis thaliana, partial (78%)
Length = 1013
Score = 30.4 bits (67), Expect = 0.32
Identities = 27/93 (29%), Positives = 37/93 (39%)
Frame = +2
Query: 17 FTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELNES 76
+ P PP + FN L P P L L A + +NP+P P P P L E
Sbjct: 83 YIPVPPQ----KPFNIPLTP--PLLPDFSPSPLQHAASMSEIKINNPDPRPMP*PILEEE 244
Query: 77 ELASELKKAMQERKEQEGNNFWNGVVSEIGEIE 109
+ EL+ RK + N G V+ +E
Sbjct: 245 D-DEELENEGSLRKRRSNRNVGVGGVNSSRRLE 340
>TC83965 weakly similar to GP|21592320|gb|AAM64271.1 unknown {Arabidopsis
thaliana}, partial (6%)
Length = 745
Score = 30.4 bits (67), Expect = 0.32
Identities = 15/38 (39%), Positives = 24/38 (62%)
Frame = +3
Query: 8 SFTLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTR 45
+++L+LSP T SP T++ F +KPSLP L ++
Sbjct: 135 TWSLSLSPPNTSSPSPTVTNSLSLFLIKPSLPFLASSK 248
>BI271192
Length = 503
Score = 30.4 bits (67), Expect = 0.32
Identities = 15/43 (34%), Positives = 24/43 (54%)
Frame = +2
Query: 46 RCTLPRAVEEKDQPTSNPEPEPEPKPELNESELASELKKAMQE 88
RCTL ++ E T + E + ELN+SEL+S + ++ E
Sbjct: 74 RCTLIHSISESQNLTPTIGAQTETETELNQSELSSLNRNSISE 202
>BE324536 similar to GP|7299263|gb| CG9461 gene product {Drosophila
melanogaster}, partial (1%)
Length = 361
Score = 30.4 bits (67), Expect = 0.32
Identities = 20/93 (21%), Positives = 36/93 (38%)
Frame = +1
Query: 9 FTLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPE 68
F + +P F T F N + P L L T + P + + NP EP+
Sbjct: 76 FWMLRNPSFPQPNLHTADQLFVNGVILP-LHLLPTTSKTDPPPQTPTSNPSSHNPVSEPD 252
Query: 69 PKPELNESELASELKKAMQERKEQEGNNFWNGV 101
P+P+ + E + + ++ G+ W +
Sbjct: 253 PEPDSSHPESSPVITESSSSASTFSGSKRWKDI 351
>TC82543 similar to GP|9294225|dbj|BAB02127.1 gene_id:K17E12.14~unknown
protein {Arabidopsis thaliana}, partial (20%)
Length = 718
Score = 30.0 bits (66), Expect = 0.41
Identities = 17/60 (28%), Positives = 26/60 (43%), Gaps = 1/60 (1%)
Frame = +2
Query: 39 PSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQ-EGNNF 97
P +L+ R LP++ +P S +P P P P + S KE+ +GN F
Sbjct: 449 PFTSLSIRIFLPQSALSPPEPNSKSKPNPNPNPNYEGGSVNSRRNSYGPPYKEEVKGNGF 628
>BF647189 similar to GP|8885590|dbj| gene_id:F24B18.9~pir||T05755~similar to
unknown protein {Arabidopsis thaliana}, partial (27%)
Length = 467
Score = 29.6 bits (65), Expect = 0.54
Identities = 15/43 (34%), Positives = 19/43 (43%)
Frame = +3
Query: 35 KPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELNESE 77
KP + RC + EE PT P P P P P LN ++
Sbjct: 294 KPPVLGSVTRFRCVIDAPAEENSTPT--PTPTPTPVPALNNND 416
>TC77478 similar to GP|9884651|dbj|BAB11979.1 MGDG synthase type A {Glycine
max}, partial (85%)
Length = 2229
Score = 29.6 bits (65), Expect = 0.54
Identities = 24/75 (32%), Positives = 31/75 (41%)
Frame = -2
Query: 2 VLQAKTSFTLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTS 61
+++ KT F SP F P+P S+ +P F F S PS PT
Sbjct: 896 LIKIKTFFAFFPSPLFNPNPSSS-TPTPFPFP-PTSFPS------------------PTP 777
Query: 62 NPEPEPEPKPELNES 76
P P P P LNE+
Sbjct: 776 PPPPPSPPSPTLNEA 732
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.312 0.132 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,849,630
Number of Sequences: 36976
Number of extensions: 76262
Number of successful extensions: 1028
Number of sequences better than 10.0: 120
Number of HSP's better than 10.0 without gapping: 844
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 964
length of query: 159
length of database: 9,014,727
effective HSP length: 88
effective length of query: 71
effective length of database: 5,760,839
effective search space: 409019569
effective search space used: 409019569
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC136286.14