BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136286.10 + phase: 0
(449 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77114 similar to GP|6686786|emb|CAB64720.1 AKIN gamma {Arabido... 258 3e-69
TC77118 similar to GP|6686786|emb|CAB64720.1 AKIN gamma {Arabido... 141 4e-34
TC77116 similar to GP|9294182|dbj|BAB02084.1 protein kinases-lik... 64 9e-11
CA989491 similar to PIR|T10971|T109 Pv42 protein - kidney bean, ... 39 0.003
TC76819 similar to SP|Q9LEV3|UMP3_ARATH Protein At5g10860 mitoc... 36 0.026
TC89183 weakly similar to PIR|F70591|F70591 probable kefB protei... 35 0.077
TC78567 similar to PIR|T04143|T04143 CLB1 protein - tomato, part... 32 0.50
TC87439 weakly similar to GP|5139695|dbj|BAA81686.1 expressed in... 32 0.50
TC77143 similar to GP|559557|gb|AAA51649.1|| arabinogalactan-pro... 32 0.65
TC85251 homologue to PIR|JQ2128|JQ2128 metallothionein - soybean... 30 1.5
BQ144502 weakly similar to GP|6523547|emb hydroxyproline-rich gl... 29 4.2
TC82303 weakly similar to GP|18073451|emb|CAC83345. GAS-2 homolo... 29 4.2
TC87262 similar to GP|9759069|dbj|BAB09547.1 gene_id:MGN6.11~pir... 28 5.5
TC81701 similar to SP|Q9C0T1|RL37_EMENI 60S ribosomal protein L3... 28 7.2
TC86849 similar to GP|14190431|gb|AAK55696.1 AT5g02770/F9G14_80 ... 28 9.4
TC85985 similar to GP|5139695|dbj|BAA81686.1 expressed in cucumb... 28 9.4
AW980936 similar to GP|14190431|gb| AT5g02770/F9G14_80 {Arabidop... 28 9.4
TC88156 similar to GP|17132837|dbj|BAB75402. ORF_ID:alr3703~hypo... 28 9.4
>TC77114 similar to GP|6686786|emb|CAB64720.1 AKIN gamma {Arabidopsis
thaliana}, partial (85%)
Length = 1417
Score = 258 bits (659), Expect = 3e-69
Identities = 150/427 (35%), Positives = 241/427 (56%), Gaps = 5/427 (1%)
Frame = +2
Query: 4 VQNMAHEQEVRTSTQLSKCDRYFETIQSRKKLPQTLQETLTDSFAKIPVSSFPGVPGGKV 63
V MA +E+ S + +K E + ++ + + L F IPVS+FP +
Sbjct: 23 VMAMATMREMPRSPE-AKLGMRVEDLWDVQEAQLSPNDKLNACFESIPVSAFPLPAKNQE 199
Query: 64 VEILADTPVGEAVKILSESNILAAPVKDPDAGIGSDWRDRYLGIIDYSAIILWVMESAEL 123
+EI +D + EAVKIL+ NIL+APV D DA + W DRY+GI++++ I++W++ +E
Sbjct: 200 IEIKSDATLAEAVKILARHNILSAPVVDVDAPEDATWIDRYIGIVEFAGIVVWILHQSEP 379
Query: 124 AAVALSAGTATAAGVGAGTVGALGAIALGATGPAAIAGLTAAAVGAAVVGGVAADKTMAK 183
+ S T T+A AIA A G L A +G+A
Sbjct: 380 PS-PRSPSTPTSA----------SAIAAAANGNTFARELEALGLGSAA------------ 490
Query: 184 DAPQAANNLGEDFYKVILQEEPFKSTTVRSILKSYRWAPFVPVAKNSAMLTVLLLLSKYR 243
+F++ + E +K+T V+ I ++RWAPF+ + ++++ LT+LLLLSKY+
Sbjct: 491 -------TTSGNFFEDLTSSELYKNTKVQDISGTFRWAPFLALERSNSFLTMLLLLSKYK 649
Query: 244 LRNVPVIEPGKADIVNFITQSAVIQGLEGCRGRDWFDCIAARPMADLGLPFMSADKVISI 303
+++VPV++ G I N ITQ AVI L C G WF+ + ++D+GLP ++ ++I +
Sbjct: 650 MKSVPVVDLGSGTIDNIITQPAVIHMLAECAGLQWFESWGTKELSDVGLPLVTPKQIIKV 829
Query: 304 QSNELILEAFKIMRDNQIGGLPVVEGPAKTIVGNLSIRDIRYLLLKPEIFSNFRNLTVMD 363
+E +L+AFK MR ++GG+PV++ T VGN+S+RD+++LL PEI+ ++R +TV D
Sbjct: 830 YEDEPVLQAFKEMRKKRVGGVPVIKRGGTTAVGNISLRDVQFLLTAPEIYHDYRTITVKD 1009
Query: 364 FMKKIVSASYESGKVTRP-----ITCKPDATLQSVIHTLASQSIHRIYTVNGQDQVVGVI 418
F+ V + E K P ITCK D T++ +I L + IHR+Y V+ + G+I
Sbjct: 1010FLTS-VRSYLEKNKNAFPMSSEFITCKRDCTVKELIQLLDKEQIHRVYVVDDDGNLEGLI 1186
Query: 419 TLRDVIS 425
TLRD+IS
Sbjct: 1187TLRDIIS 1207
>TC77118 similar to GP|6686786|emb|CAB64720.1 AKIN gamma {Arabidopsis
thaliana}, partial (52%)
Length = 1534
Score = 141 bits (356), Expect = 4e-34
Identities = 62/147 (42%), Positives = 105/147 (71%)
Frame = +3
Query: 211 VRSILKSYRWAPFVPVAKNSAMLTVLLLLSKYRLRNVPVIEPGKADIVNFITQSAVIQGL 270
V+ I +++RWAPF+ + ++++ LT+LLLLSKY++++VPV++ G I N ITQ AVI L
Sbjct: 138 VQDISRTFRWAPFLALERSNSFLTMLLLLSKYKMKSVPVVDLGSGTIDNIITQPAVIHML 317
Query: 271 EGCRGRDWFDCIAARPMADLGLPFMSADKVISIQSNELILEAFKIMRDNQIGGLPVVEGP 330
C G WF+ + ++D+GLP ++ ++I + +E +L+AFK MR ++GG+PV++
Sbjct: 318 AECAGLQWFESWGTKELSDVGLPLVTPKQIIKVYEDEPVLQAFKEMRKKKVGGVPVIKRG 497
Query: 331 AKTIVGNLSIRDIRYLLLKPEIFSNFR 357
T VGN+S+RD+++LL PEI+ ++R
Sbjct: 498 GTTAVGNISLRDVQFLLTAPEIYHDYR 578
Score = 62.4 bits (150), Expect(2) = 1e-10
Identities = 34/88 (38%), Positives = 50/88 (56%), Gaps = 5/88 (5%)
Frame = +2
Query: 357 RNLTVMDFMKKIVSASYESGKVTRP-----ITCKPDATLQSVIHTLASQSIHRIYTVNGQ 411
R +TV DF+ + S E K P ITCK D T++ +I L + IHR+Y V+
Sbjct: 836 RTITVKDFLTSVRSY-LEKNKNAFPMSSEFITCKRDCTVKELIQLLDKEQIHRVYVVDDD 1012
Query: 412 DQVVGVITLRDVISCFITEPDYHFDDYY 439
+ G+ITLRD+IS + EP +F D++
Sbjct: 1013GNLEGLITLRDIISRLVHEPHGYFGDFF 1096
Score = 21.2 bits (43), Expect(2) = 1e-10
Identities = 6/18 (33%), Positives = 16/18 (88%)
Frame = +1
Query: 337 NLSIRDIRYLLLKPEIFS 354
++++RDI+ L+ +PE+++
Sbjct: 667 SITVRDIKNLVSRPELWT 720
>TC77116 similar to GP|9294182|dbj|BAB02084.1 protein kinases-like protein
{Arabidopsis thaliana}, partial (28%)
Length = 724
Score = 64.3 bits (155), Expect = 9e-11
Identities = 41/113 (36%), Positives = 60/113 (52%)
Frame = +2
Query: 4 VQNMAHEQEVRTSTQLSKCDRYFETIQSRKKLPQTLQETLTDSFAKIPVSSFPGVPGGKV 63
V MA +E+ S + +K E + ++ + + L F IPVS+FP +
Sbjct: 5 VMAMATMREMPRSPE-AKLGMRVEDLWDVQEAQLSPNDKLNACFESIPVSAFPLPAKNQE 181
Query: 64 VEILADTPVGEAVKILSESNILAAPVKDPDAGIGSDWRDRYLGIIDYSAIILW 116
+EI +D + EAVKIL+ NIL+APV D DA + W DRY+GI +I W
Sbjct: 182 IEIKSDATLAEAVKILARHNILSAPVVDVDAPEDATWIDRYIGIC*EIKVICW 340
>CA989491 similar to PIR|T10971|T109 Pv42 protein - kidney bean, partial
(14%)
Length = 352
Score = 39.3 bits (90), Expect = 0.003
Identities = 16/43 (37%), Positives = 28/43 (64%)
Frame = +3
Query: 382 ITCKPDATLQSVIHTLASQSIHRIYTVNGQDQVVGVITLRDVI 424
+TC ++TL VI + +HR++ V+ + +VGV++L DVI
Sbjct: 39 VTCYAESTLSEVIDKAVANHVHRVWVVDQEGLLVGVVSLTDVI 167
>TC76819 similar to SP|Q9LEV3|UMP3_ARATH Protein At5g10860 mitochondrial
precursor. [Mouse-ear cress] {Arabidopsis thaliana},
partial (82%)
Length = 975
Score = 36.2 bits (82), Expect = 0.026
Identities = 34/152 (22%), Positives = 72/152 (47%), Gaps = 9/152 (5%)
Frame = +1
Query: 201 LQEEPFKSTTVRSILK--------SYRWAPFVPVAKNSAMLTVLLLLSKYRLRNVPVIEP 252
++E F+STT+ ILK S+ W + + + +++ + + V++P
Sbjct: 235 IEEHGFESTTISDILKGKGKGADGSWLWC-----TTDDTVYDAVKSMTQNNVGALVVVKP 399
Query: 253 GKA-DIVNFITQSAVIQGLEGCRGRDWFDCIAARPMADLGLPFMSADKVISIQSNELILE 311
G+ I IT+ ++ + +GR + +G +K+I++ + +L
Sbjct: 400 GEEKSIAGIITERDYLRKII-VQGR-------SSKSTKVGDIMTEENKLITVTPDTKVLR 555
Query: 312 AFKIMRDNQIGGLPVVEGPAKTIVGNLSIRDI 343
A ++M DN+I +PV+ K ++G +SI D+
Sbjct: 556 AMQLMTDNRIRHIPVIND--KGMLGMVSIGDV 645
>TC89183 weakly similar to PIR|F70591|F70591 probable kefB protein -
Mycobacterium tuberculosis (strain H37RV), partial (7%)
Length = 1137
Score = 34.7 bits (78), Expect = 0.077
Identities = 22/54 (40%), Positives = 30/54 (54%), Gaps = 3/54 (5%)
Frame = +1
Query: 123 LAAVALSAGTATAAG---VGAGTVGALGAIALGATGPAAIAGLTAAAVGAAVVG 173
+AAVA +AG ATAAG VG G V A G +AL + G+ + A+ +G
Sbjct: 646 VAAVASTAGAATAAGAVLVGTGVVAAAGVLALDLLALHLVEGIARSVNVASGLG 807
>TC78567 similar to PIR|T04143|T04143 CLB1 protein - tomato, partial (53%)
Length = 1159
Score = 32.0 bits (71), Expect = 0.50
Identities = 20/51 (39%), Positives = 25/51 (48%)
Frame = +3
Query: 119 ESAELAAVALSAGTATAAGVGAGTVGALGAIALGATGPAAIAGLTAAAVGA 169
E +L A + T A G G G VG+ I L TG A AGL + +GA
Sbjct: 612 ERKKLKAAGVIGSTMDAVGSGVGLVGS--GIGLVGTGIGAGAGLVGSGIGA 758
>TC87439 weakly similar to GP|5139695|dbj|BAA81686.1 expressed in cucumber
hypocotyls {Cucumis sativus}, partial (57%)
Length = 897
Score = 32.0 bits (71), Expect = 0.50
Identities = 25/87 (28%), Positives = 34/87 (38%), Gaps = 10/87 (11%)
Frame = -3
Query: 94 AGIGSDWRDRYLGIIDYSAIILWVMESAELAAVALSAGTATAAGV----------GAGTV 143
AG+G D+ G ++ V + AA ++ G A V GAG V
Sbjct: 361 AGVGGDFTGTGAG----GGVLTGVTAGGDEAAGGVAVGAAALGDVAGGDDLGEATGAGVV 194
Query: 144 GALGAIALGATGPAAIAGLTAAAVGAA 170
G +G A G AG+ VGAA
Sbjct: 193 GVVGGAATGDETGVVAAGVDGEVVGAA 113
>TC77143 similar to GP|559557|gb|AAA51649.1|| arabinogalactan-protein {Pyrus
communis}, partial (30%)
Length = 956
Score = 31.6 bits (70), Expect = 0.65
Identities = 19/41 (46%), Positives = 22/41 (53%)
Frame = -2
Query: 136 AGVGAGTVGALGAIALGATGPAAIAGLTAAAVGAAVVGGVA 176
+GVGA VG G A +AG+ AAA G VGGVA
Sbjct: 385 SGVGASEVGVGAG---GEVAGAGVAGVGAAAGGGVAVGGVA 272
Score = 30.0 bits (66), Expect = 1.9
Identities = 19/45 (42%), Positives = 23/45 (50%), Gaps = 1/45 (2%)
Frame = -2
Query: 133 ATAAGVGAG-TVGALGAIALGATGPAAIAGLTAAAVGAAVVGGVA 176
A+ GVGAG V G +GA +A + AVG VGGVA
Sbjct: 373 ASEVGVGAGGEVAGAGVAGVGAAAGGGVA-VGGVAVGGVAVGGVA 242
Score = 27.7 bits (60), Expect = 9.4
Identities = 26/67 (38%), Positives = 31/67 (45%), Gaps = 4/67 (5%)
Frame = -2
Query: 126 VALSAGTATAAGVGAGTVGAL--GAIALG--ATGPAAIAGLTAAAVGAAVVGGVAADKTM 181
V + AG AG G VGA G +A+G A G A+ G+ A GA V G AD
Sbjct: 364 VGVGAG-GEVAGAGVAGVGAAAGGGVAVGGVAVGGVAVGGV---ATGAGVGGVGVADGGC 197
Query: 182 AKDAPQA 188
AP A
Sbjct: 196 VGAAPGA 176
>TC85251 homologue to PIR|JQ2128|JQ2128 metallothionein - soybean, partial
(93%)
Length = 1554
Score = 30.4 bits (67), Expect = 1.5
Identities = 22/47 (46%), Positives = 25/47 (52%), Gaps = 1/47 (2%)
Frame = +1
Query: 130 AGT-ATAAGVGAGTVGALGAIALGATGPAAIAGLTAAAVGAAVVGGV 175
AGT A+ AGVGAG G + +GA A AG A GA GGV
Sbjct: 430 AGTGASIAGVGAGIGGEVAGAGVGACFAGAGAGTGVAFTGAG-AGGV 567
Score = 28.1 bits (61), Expect = 7.2
Identities = 15/38 (39%), Positives = 19/38 (49%)
Frame = +1
Query: 139 GAGTVGALGAIALGATGPAAIAGLTAAAVGAAVVGGVA 176
GAGT ++ + G G A AG+ A GA GVA
Sbjct: 427 GAGTGASIAGVGAGIGGEVAGAGVGACFAGAGAGTGVA 540
>BQ144502 weakly similar to GP|6523547|emb hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (39%)
Length = 1358
Score = 28.9 bits (63), Expect = 4.2
Identities = 20/50 (40%), Positives = 24/50 (48%)
Frame = +1
Query: 124 AAVALSAGTATAAGVGAGTVGALGAIALGATGPAAIAGLTAAAVGAAVVG 173
A + AG A A GVG G GA G A G A+AG +A G + G
Sbjct: 100 ACGGVGAGGAAAGGVG-GGAGAEGGRRRRARGGGAVAGGMPSAAGGLLRG 246
>TC82303 weakly similar to GP|18073451|emb|CAC83345. GAS-2 homologue
{Candida glabrata}, partial (7%)
Length = 846
Score = 28.9 bits (63), Expect = 4.2
Identities = 23/60 (38%), Positives = 32/60 (53%)
Frame = -2
Query: 121 AELAAVALSAGTATAAGVGAGTVGALGAIALGATGPAAIAGLTAAAVGAAVVGGVAADKT 180
A + VA SAG + A+G+G GT G G+ A+G G +GL A G G+A + T
Sbjct: 428 ATSSGVAGSAGGSAASGIG-GTGGG-GSDAVGG-GSGGESGLARAVAGGGAGLGLAVEVT 258
>TC87262 similar to GP|9759069|dbj|BAB09547.1
gene_id:MGN6.11~pir||T08938~strong similarity to unknown
protein {Arabidopsis thaliana}, partial (20%)
Length = 1481
Score = 28.5 bits (62), Expect = 5.5
Identities = 13/60 (21%), Positives = 32/60 (52%), Gaps = 2/60 (3%)
Frame = +1
Query: 370 SASYESGKVTRP--ITCKPDATLQSVIHTLASQSIHRIYTVNGQDQVVGVITLRDVISCF 427
S+SY + V + I C P ++L +V+ + ++ ++ + +VG++T +++ F
Sbjct: 1024 SSSYSAKYVRKAEAIVCHPKSSLIAVMMQAIANRVNYLWVIEDDWSLVGIVTFSNILKVF 1203
>TC81701 similar to SP|Q9C0T1|RL37_EMENI 60S ribosomal protein L37.
[Aspergillus nidulans] {Emericella nidulans}, partial
(92%)
Length = 720
Score = 28.1 bits (61), Expect = 7.2
Identities = 25/70 (35%), Positives = 27/70 (37%)
Frame = -3
Query: 121 AELAAVALSAGTATAAGVGAGTVGALGAIALGATGPAAIAGLTAAAVGAAVVGGVAADKT 180
A L AV +AG A GA G ALG GP +AGL GV D
Sbjct: 325 AGLEAVLEAAGDVLEV---AHATGAGGLSALGLLGPVVLAGLGRGVTAGR--AGVLLDVK 161
Query: 181 MAKDAPQAAN 190
A A A N
Sbjct: 160 RAATAATAQN 131
>TC86849 similar to GP|14190431|gb|AAK55696.1 AT5g02770/F9G14_80
{Arabidopsis thaliana}, partial (30%)
Length = 1788
Score = 27.7 bits (60), Expect = 9.4
Identities = 19/60 (31%), Positives = 31/60 (51%)
Frame = -2
Query: 117 VMESAELAAVALSAGTATAAGVGAGTVGALGAIALGATGPAAIAGLTAAAVGAAVVGGVA 176
V S + ++ LS+ + +AG+G+ + LG + + + AGL V AAV GVA
Sbjct: 260 VSGSVGVVSLDLSSSASLSAGIGSDSEWGLGEVGSRVSRGFSSAGLPLVVV-AAVAEGVA 84
>TC85985 similar to GP|5139695|dbj|BAA81686.1 expressed in cucumber
hypocotyls {Cucumis sativus}, partial (42%)
Length = 892
Score = 27.7 bits (60), Expect = 9.4
Identities = 24/67 (35%), Positives = 28/67 (40%), Gaps = 1/67 (1%)
Frame = -1
Query: 131 GTATAAGVGAGTVGA-LGAIALGATGPAAIAGLTAAAVGAAVVGGVAADKTMAKDAPQAA 189
G T G G GA G + GAT AG A GAA G A T+A +A
Sbjct: 412 GELTGTGAGGELTGAGFGGVLTGATTGDDAAGDLATGTGAA---GETAGVTVAAVGDEAG 242
Query: 190 NNLGEDF 196
G+DF
Sbjct: 241 ---GDDF 230
>AW980936 similar to GP|14190431|gb| AT5g02770/F9G14_80 {Arabidopsis
thaliana}, partial (25%)
Length = 565
Score = 27.7 bits (60), Expect = 9.4
Identities = 19/60 (31%), Positives = 31/60 (51%)
Frame = -3
Query: 117 VMESAELAAVALSAGTATAAGVGAGTVGALGAIALGATGPAAIAGLTAAAVGAAVVGGVA 176
V S + ++ LS+ + +AG+G+ + LG + + + AGL V AAV GVA
Sbjct: 236 VSGSVGVVSLDLSSSASLSAGIGSDSEWGLGEVGSRVSRGFSSAGLPLVVV-AAVAEGVA 60
>TC88156 similar to GP|17132837|dbj|BAB75402. ORF_ID:alr3703~hypothetical
protein {Nostoc sp. PCC 7120}, partial (5%)
Length = 673
Score = 27.7 bits (60), Expect = 9.4
Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 1/48 (2%)
Frame = +2
Query: 125 AVALSAGTATAAGVGAGTVGALGAIALGATGPAAIAGLTAA-AVGAAV 171
AV SAG A V G G +G + +GP + G A ++G A+
Sbjct: 368 AVLASAGKALIVRVPVGVAGIVGIGTVAKSGPVLLGGFVGAYSLGVAL 511
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.135 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,742,937
Number of Sequences: 36976
Number of extensions: 121660
Number of successful extensions: 681
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 654
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 678
length of query: 449
length of database: 9,014,727
effective HSP length: 99
effective length of query: 350
effective length of database: 5,354,103
effective search space: 1873936050
effective search space used: 1873936050
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC136286.10