BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135959.2 - phase: 0
(479 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB065382 similar to PIR|T09671|T096 RPE15 protein - alfalfa (fra... 322 2e-88
TC89839 homologue to SP|P23466|CYAA_SACKL Adenylate cyclase (EC ... 83 3e-16
AW684517 similar to GP|9927273|dbj Similar to Arabidopsis thalia... 75 6e-14
AL375832 72 5e-13
BE124481 65 7e-11
TC82044 similar to PIR|B86285|B86285 hypothetical protein AAD396... 35 0.049
BQ144376 homologue to GP|15419013|gb| subtilisin-like protein {T... 35 0.083
TC90796 similar to GP|3395431|gb|AAC28763.1| unknown protein {Ar... 35 0.083
TC81537 similar to GP|7630037|emb|CAB88331.1 putative protein {A... 34 0.14
TC80046 similar to SP|P22699|GUN6_DICDI Endoglucanase precursor ... 33 0.18
TC89797 GP|13775543|gb|AAK39351.1 Hypothetical protein Y73B3A.17... 33 0.18
BQ141932 weakly similar to GP|6523547|emb hydroxyproline-rich gl... 33 0.24
TC78495 homologue to PIR|S53504|S53504 extensin-like protein S3 ... 33 0.24
CA859989 similar to GP|18308125|gb bromodomain-containing protei... 33 0.24
TC83704 similar to PIR|T05005|T05005 hypothetical protein T19P19... 32 0.41
TC79093 similar to GP|4102190|gb|AAD01431.1| 35 kDa seed maturat... 32 0.41
BQ141911 similar to GP|11036868|gb| PxORF73 peptide {Plutella xy... 32 0.54
BQ141909 weakly similar to GP|14253159|emb VMP3 protein {Volvox ... 32 0.54
TC91076 similar to PIR|H96747|H96747 unknown protein T10D10.14 [... 32 0.54
BQ752151 similar to GP|1945155|emb MN1 {Homo sapiens}, partial (1%) 32 0.70
>CB065382 similar to PIR|T09671|T096 RPE15 protein - alfalfa (fragment),
partial (9%)
Length = 624
Score = 322 bits (826), Expect = 2e-88
Identities = 158/189 (83%), Positives = 165/189 (86%), Gaps = 1/189 (0%)
Frame = -2
Query: 190 QPQVPVNAVVQQMQQQPPA-QQQQHQQARPTFPPIPMLYAELLPTLLLRGHYTTRQGKPP 248
+P +P PP QQ+Q QQARPTFP IPMLYAE LPTLLLRGH T RQGKPP
Sbjct: 569 RPMIP*LLC*PPRNNHPPVHQQRQ*QQARPTFPLIPMLYAE*LPTLLLRGHCTIRQGKPP 390
Query: 249 PDPLPPRFRSDLKCDFHQGALGHDVEGCYALKYIVKKLINQGKLTFENNIPHVLDNPLPN 308
PDPLPPRFRSDLKCDFHQGALGHDVEGCYALK+IVKKLINQGKLTFENN+PHVLDNPLPN
Sbjct: 389 PDPLPPRFRSDLKCDFHQGALGHDVEGCYALKHIVKKLINQGKLTFENNVPHVLDNPLPN 210
Query: 309 HAAVNMIEVYEEAPRLDVRNVTTPLVPLHIKLCQASLFDHDHASCPECFCNPLGYCVVQN 368
HAAVNMIEVYEEAP LDVRNVTTPLVPLHIKLCQASLFDHDHA+C E F NPLG CVVQN
Sbjct: 209 HAAVNMIEVYEEAPGLDVRNVTTPLVPLHIKLCQASLFDHDHANCQE*FYNPLGCCVVQN 30
Query: 369 DIQSLMNDN 377
DIQSLMN+N
Sbjct: 29 DIQSLMNNN 3
Score = 68.9 bits (167), Expect = 4e-12
Identities = 35/42 (83%), Positives = 38/42 (90%)
Frame = -1
Query: 3 EENAQLRTELASLREELAKANNTMTALLAAQEQSATAIPVAT 44
EENAQLRTELAS+REELAKAN+TMTALLAAQEQS+T P T
Sbjct: 624 EENAQLRTELASMREELAKANDTMTALLAAQEQSSTGSPTTT 499
>TC89839 homologue to SP|P23466|CYAA_SACKL Adenylate cyclase (EC 4.6.1.1)
(ATP pyrophosphate-lyase) (Adenylyl cyclase). [Yeast],
partial (0%)
Length = 665
Score = 82.8 bits (203), Expect = 3e-16
Identities = 52/132 (39%), Positives = 62/132 (46%), Gaps = 7/132 (5%)
Frame = +3
Query: 178 PPFYHQYPLPSGQPQVPVNAVVQQMQQQPPAQQ-------QQHQQARPTFPPIPMLYAEL 230
PP Q P QP P Q +Q P Q Q Q PIP+ YA+L
Sbjct: 228 PPLVQQRPQSPYQPMCPQKPRQQAPRQSIPQNQVPQKFIPQNQVQKASQCDPIPVKYADL 407
Query: 231 LPTLLLRGHYTTRQGKPPPDPLPPRFRSDLKCDFHQGALGHDVEGCYALKYIVKKLINQG 290
LP LL + T P+ LPP +R DL C FHQGA GHD E CY LK V+KLI
Sbjct: 408 LPILLKKNLIQTLPLPRVPNSLPPWYRPDLNCVFHQGAPGHDTEQCYPLKEEVQKLIENN 587
Query: 291 KLTFENNIPHVL 302
+F++ VL
Sbjct: 588 VWSFDDQDIKVL 623
>AW684517 similar to GP|9927273|dbj Similar to Arabidopsis thaliana
chromosome II BAC F26H6; putative retroelement pol
polyprotein, partial (1%)
Length = 488
Score = 75.1 bits (183), Expect = 6e-14
Identities = 32/49 (65%), Positives = 40/49 (81%)
Frame = +1
Query: 430 ATGIFYGAEFVNAITEEATGFGPRPVFVIPGGIARNWDAIDIPSIMHVS 478
+TG F+ A FVN + EE F PRP+FVIPGGIA++WDA+D+PSIMHVS
Sbjct: 319 STGTFHSAGFVNTLAEEVARFVPRPLFVIPGGIAKDWDAVDVPSIMHVS 465
>AL375832
Length = 535
Score = 72.0 bits (175), Expect = 5e-13
Identities = 34/41 (82%), Positives = 36/41 (86%)
Frame = +3
Query: 439 FVNAITEEATGFGPRPVFVIPGGIARNWDAIDIPSIMHVSE 479
FVNAI+EEATG G RP FV PGGIA +WDAIDIPSIMHVSE
Sbjct: 3 FVNAISEEATGSGLRPAFVTPGGIASDWDAIDIPSIMHVSE 125
>BE124481
Length = 538
Score = 64.7 bits (156), Expect = 7e-11
Identities = 40/102 (39%), Positives = 46/102 (44%), Gaps = 7/102 (6%)
Frame = +3
Query: 178 PPFYHQYPLPSGQPQVPVNAVVQQMQQQPPAQQ-------QQHQQARPTFPPIPMLYAEL 230
PP Q P QP P Q +Q P Q Q Q PIP+ YA+L
Sbjct: 231 PPLVQQRPQSPYQPMCPQKPRQQAPRQSIPQNQVPQKFIPQNQVQKASQCDPIPVKYADL 410
Query: 231 LPTLLLRGHYTTRQGKPPPDPLPPRFRSDLKCDFHQGALGHD 272
LP LL + T P+ LPP +R DL C FHQGA GHD
Sbjct: 411 LPILLKKNLIQTLPLPRVPNSLPPWYRPDLNCVFHQGAPGHD 536
>TC82044 similar to PIR|B86285|B86285 hypothetical protein AAD39642.1
[imported] - Arabidopsis thaliana, partial (16%)
Length = 950
Score = 35.4 bits (80), Expect = 0.049
Identities = 32/98 (32%), Positives = 38/98 (38%), Gaps = 6/98 (6%)
Frame = +1
Query: 160 AQLPPSYPYAQYSQHPFFPPFYHQYPLPSGQPQVPVNAVVQQMQQQPPAQQQQHQQARPT 219
A PPSY ++ + PP +Q P S P P Q QQQPPA H +P
Sbjct: 553 AHPPPSYGHSHHQ-----PPPPYQIPPTSTAPYPPP----QVHQQQPPA---NHDYGQPA 696
Query: 220 FPPIPMLYAELLPTLLL------RGHYTTRQGKPPPDP 251
+P Y PT R YT PPP P
Sbjct: 697 YPGWRAPYYNNAPTHPQQQQPGPRPPYTIPSPYPPPSP 810
>BQ144376 homologue to GP|15419013|gb| subtilisin-like protein {Toxoplasma
gondii}, partial (3%)
Length = 744
Score = 34.7 bits (78), Expect = 0.083
Identities = 25/73 (34%), Positives = 31/73 (42%), Gaps = 2/73 (2%)
Frame = +3
Query: 180 FYHQYPLPSGQPQVPVNAVVQQMQQQPPAQQ-QQHQQARPTFPPIPMLYAELLPTLLLRG 238
F+H P PS P +P+ PP Q H + P FPP P + P LL R
Sbjct: 456 FFHDTPPPS--PLLPL----------PPRQLLNNHSRLLPPFPPSPHPFLSFPPRLLCRF 599
Query: 239 HYT-TRQGKPPPD 250
H+ PPPD
Sbjct: 600 HFPFFSTFSPPPD 638
>TC90796 similar to GP|3395431|gb|AAC28763.1| unknown protein {Arabidopsis
thaliana}, partial (20%)
Length = 965
Score = 34.7 bits (78), Expect = 0.083
Identities = 25/66 (37%), Positives = 30/66 (44%), Gaps = 8/66 (12%)
Frame = +2
Query: 165 SYPYAQYSQHPFFPPFYHQYPLPSGQPQVPVNAVVQQMQQQ--------PPAQQQQHQQA 216
SY YA + +P P Y+ Y P QPQ QQ QQ PP+ QQ Q
Sbjct: 773 SYSYAPQA-YPGNSP-YNSYVAPWAQPQSKSEFQTQQPQQHMYQPQPTTPPSPQQHMYQP 946
Query: 217 RPTFPP 222
+PT PP
Sbjct: 947 QPTTPP 964
>TC81537 similar to GP|7630037|emb|CAB88331.1 putative protein {Arabidopsis
thaliana}, partial (34%)
Length = 751
Score = 33.9 bits (76), Expect = 0.14
Identities = 22/76 (28%), Positives = 34/76 (43%), Gaps = 2/76 (2%)
Frame = -1
Query: 149 LITFQVMDINAAQLPPSYPYAQYSQHPFFPPFYHQYP--LPSGQPQVPVNAVVQQMQQQP 206
LI+FQ + ++ P +YP + H F + QYP PS P ++ + + P
Sbjct: 751 LISFQTIHCTVSKTPYTYPN---THHQFQF*HHFQYPPTSPSLPPSYALHE*TRALHDPP 581
Query: 207 PAQQQQHQQARPTFPP 222
P + A PT PP
Sbjct: 580 PTLSAANPSAAPTSPP 533
>TC80046 similar to SP|P22699|GUN6_DICDI Endoglucanase precursor (EC
3.2.1.4) (Endo-1 4-beta-glucanase) (Spore germination
protein 270-6), partial (11%)
Length = 643
Score = 33.5 bits (75), Expect = 0.18
Identities = 30/97 (30%), Positives = 38/97 (38%)
Frame = +1
Query: 161 QLPPSYPYAQYSQHPFFPPFYHQYPLPSGQPQVPVNAVVQQMQQQPPAQQQQHQQARPTF 220
Q PP P + Q P P Q P P PQ P + Q++ Q+PP + H Q
Sbjct: 325 QRPPQKPPQRPPQRP--PQKLPQRP-PQKLPQRPPQKLPQKLPQKPPQRPLPHPQK---- 483
Query: 221 PPIPMLYAELLPTLLLRGHYTTRQGKPPPDPLPPRFR 257
PP+ L P L H +P P P P R
Sbjct: 484 PPLRPLPHPQKPPLRPLPHPQKPPLRPLPHPQKPPLR 594
Score = 31.2 bits (69), Expect = 0.92
Identities = 32/120 (26%), Positives = 44/120 (36%), Gaps = 6/120 (5%)
Frame = +1
Query: 140 PITIDPETFL-----ITFQVMDINAAQLPPSYPYAQYSQHP-FFPPFYHQYPLPSGQPQV 193
P+ + P+ +L I Q + Q PP P + Q P PP P PQ
Sbjct: 199 PLPL*PQPYLLLL*PIPLQTRSLPLPQRPPQRPPQRPPQKPPQRPPQKPPQRPPQRPPQK 378
Query: 194 PVNAVVQQMQQQPPAQQQQHQQARPTFPPIPMLYAELLPTLLLRGHYTTRQGKPPPDPLP 253
Q++ Q+PP + Q +P P+P L L KPP PLP
Sbjct: 379 LPQRPPQKLPQRPPQKLPQKLPQKPPQRPLPHPQKPPLRPL-------PHPQKPPLRPLP 537
>TC89797 GP|13775543|gb|AAK39351.1 Hypothetical protein Y73B3A.17
{Caenorhabditis elegans}, partial (1%)
Length = 958
Score = 33.5 bits (75), Expect = 0.18
Identities = 34/120 (28%), Positives = 42/120 (34%), Gaps = 11/120 (9%)
Frame = +3
Query: 163 PPSYPYAQYSQHPFFPPFYH----QYPLPSGQPQVPVNAVVQQMQQ------QPPAQQQQ 212
PPS+P+ Q FPPF+ PL S P A Q+Q + + Q
Sbjct: 219 PPSFPFPSPEQPQ*FPPFHESSHASQPLHSASSSKPH*AAATQVQDWKS*RPEYYSHPQH 398
Query: 213 HQQARPTFPPIPMLYAELLPTLLLRGHYTTRQGKPPPDPLP-PRFRSDLKCDFHQGALGH 271
H P P + PT T+ Q P P P P F S FHQ H
Sbjct: 399 HSSPSPPNQSSPSTHQSPSPT-----QSTSSQPNPYPSQAPQPYFLSQ---PFHQTVSVH 554
>BQ141932 weakly similar to GP|6523547|emb hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (30%)
Length = 1338
Score = 33.1 bits (74), Expect = 0.24
Identities = 27/91 (29%), Positives = 34/91 (36%)
Frame = -3
Query: 164 PSYPYAQYSQHPFFPPFYHQYPLPSGQPQVPVNAVVQQMQQQPPAQQQQHQQARPTFPPI 223
P +P + HP PP P+PS P P A PP+ ++ P
Sbjct: 538 PRHPEPTHQPHPCLPP-----PIPSAPP--PTRAFALPPSAPPPSDLPPPPRSPTVIDPR 380
Query: 224 PMLYAELLPTLLLRGHYTTRQGKPPPDPLPP 254
P A +P L LR Y R P P PP
Sbjct: 379 PPA-ASAVP-LPLRPLYPLRDSPPRRPPPPP 293
>TC78495 homologue to PIR|S53504|S53504 extensin-like protein S3 - alfalfa,
partial (97%)
Length = 1049
Score = 33.1 bits (74), Expect = 0.24
Identities = 24/95 (25%), Positives = 30/95 (31%)
Frame = +3
Query: 160 AQLPPSYPYAQYSQHPFFPPFYHQYPLPSGQPQVPVNAVVQQMQQQPPAQQQQHQQARPT 219
A PP+ P A PP P P P + +Q PP QQ T
Sbjct: 288 ANTPPTTPQASPPPVQSSPPPVQSSPPPLQSSPPPAQSTPPPVQSSPPPVQQSPPPTPLT 467
Query: 220 FPPIPMLYAELLPTLLLRGHYTTRQGKPPPDPLPP 254
PP+ P ++ PPP PP
Sbjct: 468 PPPVQSTPPPASPPPASPPPFSPPPATPPPATPPP 572
>CA859989 similar to GP|18308125|gb bromodomain-containing protein BRD4 long
variant {Mus musculus}, partial (1%)
Length = 375
Score = 33.1 bits (74), Expect = 0.24
Identities = 21/61 (34%), Positives = 28/61 (45%), Gaps = 7/61 (11%)
Frame = +3
Query: 198 VVQQMQQQPPAQQQQHQ-------QARPTFPPIPMLYAELLPTLLLRGHYTTRQGKPPPD 250
V QQ QQQPP +QQ+++ Q RP P Y + P Y +Q +P P
Sbjct: 48 VTQQQQQQPPPEQQRYE*PASPYHQQRPQPTPEHQRYGQPAPP------YQQQQQQPQPP 209
Query: 251 P 251
P
Sbjct: 210 P 212
>TC83704 similar to PIR|T05005|T05005 hypothetical protein T19P19.70 -
Arabidopsis thaliana, partial (15%)
Length = 716
Score = 32.3 bits (72), Expect = 0.41
Identities = 30/109 (27%), Positives = 42/109 (38%), Gaps = 7/109 (6%)
Frame = +1
Query: 154 VMDINAAQLPPSYPYAQYSQHPFFPPFYHQYPLPSGQPQVPVNAVVQQMQQQPPAQQ--- 210
V + LP PY QH PP P P ++P A +Q+ PP +
Sbjct: 268 VPPVATTSLPEPLPY----QHKEQPPIPVTLPPPPPLSKLPP-ATREQLPPPPPLSKLPP 432
Query: 211 --QQHQQARPTFPPIPMLYAELLPTL--LLRGHYTTRQGKPPPDPLPPR 255
++H P +P E LP L + R+ PPP PLP +
Sbjct: 433 AAREHPPPPPPSAKLPPAARERLPLPPPLSKLPPAARERLPPPSPLPEK 579
>TC79093 similar to GP|4102190|gb|AAD01431.1| 35 kDa seed maturation protein
{Glycine max}, partial (57%)
Length = 1127
Score = 32.3 bits (72), Expect = 0.41
Identities = 22/74 (29%), Positives = 31/74 (41%), Gaps = 2/74 (2%)
Frame = -3
Query: 164 PSYPYAQYSQHP--FFPPFYHQYPLPSGQPQVPVNAVVQQMQQQPPAQQQQHQQARPTFP 221
P+ PY Q+S + P + P PS Q + + Q PP+ QH
Sbjct: 717 PASPYQQHSSYTPQLCSPLHPSLPSPSLQHSLYTPLLYSQWYLLPPSLSLQHSLC----- 553
Query: 222 PIPMLYAELLPTLL 235
IP LY +L P+ L
Sbjct: 552 -IPRLYLQLCPSFL 514
>BQ141911 similar to GP|11036868|gb| PxORF73 peptide {Plutella xylostella
granulovirus}, partial (16%)
Length = 1151
Score = 32.0 bits (71), Expect = 0.54
Identities = 23/77 (29%), Positives = 30/77 (38%), Gaps = 13/77 (16%)
Frame = -2
Query: 163 PPSYPYAQYSQHPFFPPFYHQYPLP-----SGQPQVPVNAVVQQM--------QQQPPAQ 209
PPS+P Y+ PP H + SG P P N QQ Q PP
Sbjct: 289 PPSHPPTTYT-----PPHKHMFKTQPHVFRSGSPTQPPNQT*QQPTKHPLPQNQITPPLS 125
Query: 210 QQQHQQARPTFPPIPML 226
+ + + P PP+P L
Sbjct: 124 KHDSKTSTPNSPPLPPL 74
>BQ141909 weakly similar to GP|14253159|emb VMP3 protein {Volvox carteri f.
nagariensis}, partial (13%)
Length = 1223
Score = 32.0 bits (71), Expect = 0.54
Identities = 28/107 (26%), Positives = 43/107 (40%), Gaps = 2/107 (1%)
Frame = -3
Query: 132 NVLGEIDLPITIDPETFLITF--QVMDINAAQLPPSYPYAQYSQHPFFPPFYHQYPLPSG 189
N L + P + P + F + + +PP P +++ PF PP +PLP
Sbjct: 441 NSLFSLPFPPPLSPRLLPVPFLPSIFPLPPPSVPP--PLSRFPL-PFVPPPPF-FPLPLL 274
Query: 190 QPQVPVNAVVQQMQQQPPAQQQQHQQARPTFPPIPMLYAELLPTLLL 236
P +P ++ PP+ P FP P+L LP LL
Sbjct: 273 PPPLPPPPLLPLPLSDPPSAPPPPSPPSPRFPLSPLLLILPLPLPLL 133
>TC91076 similar to PIR|H96747|H96747 unknown protein T10D10.14 [imported] -
Arabidopsis thaliana, partial (9%)
Length = 834
Score = 32.0 bits (71), Expect = 0.54
Identities = 28/72 (38%), Positives = 30/72 (40%), Gaps = 4/72 (5%)
Frame = +3
Query: 153 QVMDINAAQLPPSYPYA-QYSQHPFFPPFYHQYPLPSGQPQVPVNAVVQQMQQQPPAQQQ 211
Q + NA LPP P Q SQ Q P Q AV+QQ QQQ QQ
Sbjct: 366 QSLVANARMLPPGNPQGLQMSQALLSGVSMAQRPQQLDSQQ----AVLQQQQQQQQLQQN 533
Query: 212 QH---QQARPTF 220
QH QQ P F
Sbjct: 534 QHSLLQQQNPQF 569
>BQ752151 similar to GP|1945155|emb MN1 {Homo sapiens}, partial (1%)
Length = 650
Score = 31.6 bits (70), Expect = 0.70
Identities = 27/95 (28%), Positives = 36/95 (37%), Gaps = 1/95 (1%)
Frame = +3
Query: 163 PPSYPYAQYSQHPFFPPFYHQYPLPSGQPQ-VPVNAVVQQMQQQPPAQQQQHQQARPTFP 221
PPS+P PP +P P Q +P +QQ QQQ QQA P P
Sbjct: 327 PPSHPPPPKQPQQQAPPPQQAWPSPHVSAQKIPPGGHLQQQTWASAPPQQQPQQAPP--P 500
Query: 222 PIPMLYAELLPTLLLRGHYTTRQGKPPPDPLPPRF 256
P M + + G+P P +P R+
Sbjct: 501 PHHMSMRD--------DRGASMYGQPHPHAMPGRY 581
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.137 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,291,857
Number of Sequences: 36976
Number of extensions: 266844
Number of successful extensions: 2321
Number of sequences better than 10.0: 93
Number of HSP's better than 10.0 without gapping: 2104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2241
length of query: 479
length of database: 9,014,727
effective HSP length: 100
effective length of query: 379
effective length of database: 5,317,127
effective search space: 2015191133
effective search space used: 2015191133
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC135959.2