BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135801.7 - phase: 0
(189 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC93217 similar to GP|13486661|dbj|BAB39898. contains EST AU0813... 54 3e-08
TC88000 weakly similar to GP|13486661|dbj|BAB39898. contains EST... 50 5e-07
AW690594 similar to GP|23498163|emb hypothetical protein {Plasmo... 35 0.023
AW257087 homologue to GP|6683126|dbj KIAA0339 protein {Homo sapi... 34 0.030
TC87549 similar to PIR|T01734|T01734 hypothetical protein A_IG00... 34 0.039
TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding... 33 0.086
TC85722 similar to SP|P08283|H1_PEA Histone H1. [Garden pea] {Pi... 32 0.11
TC92834 similar to GP|16209669|gb|AAL14393.1 AT4g02500/T10P11_20... 32 0.11
TC90105 weakly similar to GP|10241636|emb|CAC09475. putative zin... 31 0.25
TC87119 similar to GP|20197209|gb|AAM14973.1 expressed protein {... 31 0.25
TC85721 similar to SP|P08283|H1_PEA Histone H1. [Garden pea] {Pi... 31 0.25
BQ752445 similar to GP|18376387|em putative protein {Neurospora ... 31 0.25
TC84050 similar to GP|6273383|gb|AAF06346.1| SCUTL1 {Vitis vinif... 31 0.33
TC93070 weakly similar to GP|17065230|gb|AAL32769.1 Unknown prot... 31 0.33
TC89697 similar to GP|4886756|gb|AAD32031.1| cellulose synthase ... 30 0.43
AW774261 weakly similar to EGAD|146423|156 vitellogenin {Anolis ... 30 0.56
BQ137953 weakly similar to GP|22136724|gb unknown protein {Arabi... 30 0.73
BG644352 homologue to GP|12231300|gb ripening regulated protein ... 30 0.73
TC88868 30 0.73
BF638600 similar to SP|O14405|GUN4_ Endoglucanase IV precursor (... 30 0.73
>TC93217 similar to GP|13486661|dbj|BAB39898. contains EST
AU081362(R10239)~unknown protein {Oryza sativa (japonica
cultivar-group)}, partial (12%)
Length = 741
Score = 54.3 bits (129), Expect = 3e-08
Identities = 42/124 (33%), Positives = 53/124 (41%), Gaps = 1/124 (0%)
Frame = +2
Query: 33 VPFKWEMQPGIAKESSPKEVLPPLTPPPKNLSLGLPKPSILELKKPASTMSKLKFWKKRV 92
+PF WE QPG K S + LPPLTPPP KP +
Sbjct: 164 IPFVWESQPGTPKHRSNQNSLPPLTPPPSYFQNANKKP---------------------I 280
Query: 93 VKIKSKKPQEVCFHEDFDILSRLDCSSDSESMVSPRGSSFSSSSSSMSL-MKSTRSSLHS 151
K K + FH+ ++ DC D S S+SSSSSS SL + S R + +S
Sbjct: 281 TKAKKNFFLQSLFHKR---TTKKDCVLDRSPAASSTFVSYSSSSSSSSLSVSSPRPTSYS 451
Query: 152 VCSS 155
V SS
Sbjct: 452 VPSS 463
>TC88000 weakly similar to GP|13486661|dbj|BAB39898. contains EST
AU081362(R10239)~unknown protein {Oryza sativa (japonica
cultivar-group)}, partial (20%)
Length = 1096
Score = 50.1 bits (118), Expect = 5e-07
Identities = 45/135 (33%), Positives = 59/135 (43%)
Frame = +1
Query: 10 KILSRRSSVGCSSRISYYRSGEGVPFKWEMQPGIAKESSPKEVLPPLTPPPKNLSLGLPK 69
++L++ SS+ S +R VPF WE QPG K + ++ LPPLTPPP S
Sbjct: 208 RLLTKESSISNPS----FRVAVTVPFVWESQPGTPKYTFSEKNLPPLTPPPSYYSC---- 363
Query: 70 PSILELKKPASTMSKLKFWKKRVVKIKSKKPQEVCFHEDFDILSRLDCSSDSESMVSPRG 129
K P S+ + K+ KK +I+S SS S S SP
Sbjct: 364 -----KKTPIKKNSRSNIFLALFPKLNLKK----------NIMSS-SSSSPSNSPSSPYY 495
Query: 130 SSFSSSSSSMSLMKS 144
SS S SSS S S
Sbjct: 496 SSASCSSSDSSSSSS 540
>AW690594 similar to GP|23498163|emb hypothetical protein {Plasmodium
falciparum 3D7}, partial (10%)
Length = 633
Score = 34.7 bits (78), Expect = 0.023
Identities = 28/78 (35%), Positives = 36/78 (45%)
Frame = -3
Query: 111 ILSRLDCSSDSESMVSPRGSSFSSSSSSMSLMKSTRSSLHSVCSSCSEGNTKQVSRKPST 170
++ + SS S S S SS SSSSSS S S+ SS S SS S ++ S S+
Sbjct: 265 LILSISSSSPSSSSSSSSSSSSSSSSSSSSSCSSSSSSSSSSSSSSSCSSSSSSSCSSSS 86
Query: 171 LGCFPMHISRVLVSITRR 188
P S + S RR
Sbjct: 85 SSTSPSSSSSLCSSTFRR 32
>AW257087 homologue to GP|6683126|dbj KIAA0339 protein {Homo sapiens},
partial (2%)
Length = 466
Score = 34.3 bits (77), Expect = 0.030
Identities = 47/170 (27%), Positives = 63/170 (36%), Gaps = 1/170 (0%)
Frame = +1
Query: 8 SNKILSRRSSVGCSSRISYYRSGEGVPFKWEMQPGIAKESSPK-EVLPPLTPPPKNLSLG 66
S+ S SS S S + + VP + P AK +S K EV + S
Sbjct: 19 SSSSSSSSSSDSDSDSESEAPAKKAVPVVTKTAPTHAKSASSKMEVDSDSDSDSDSSSSS 198
Query: 67 LPKPSILELKKPASTMSKLKFWKKRVVKIKSKKPQEVCFHEDFDILSRLDCSSDSESMVS 126
S+ +KKPA + +K KK D SDS+S S
Sbjct: 199 STSSSVSSVKKPAVSAAKATPAKKE----------------------ESDSDSDSDSSSS 312
Query: 127 PRGSSFSSSSSSMSLMKSTRSSLHSVCSSCSEGNTKQVSRKPSTLGCFPM 176
SS SSSSSS S S+ SS +S E V + P+ P+
Sbjct: 313 SSSSSSSSSSSSSSSSSSSSSSSNSDDEEEEEEAKPVVKKTPAKSASTPV 462
>TC87549 similar to PIR|T01734|T01734 hypothetical protein A_IG002N01.30 -
Arabidopsis thaliana (fragment), partial (38%)
Length = 1766
Score = 33.9 bits (76), Expect = 0.039
Identities = 23/51 (45%), Positives = 28/51 (54%)
Frame = -3
Query: 105 FHEDFDILSRLDCSSDSESMVSPRGSSFSSSSSSMSLMKSTRSSLHSVCSS 155
F+ D S +S S S + SS SSSSSS SL+KS+ S HS SS
Sbjct: 1698 FNSDVSASSSSKSGGESSSSSSIQSSSISSSSSSSSLIKSSASD-HSCSSS 1549
Score = 27.3 bits (59), Expect = 3.6
Identities = 23/71 (32%), Positives = 31/71 (43%)
Frame = -3
Query: 118 SSDSESMVSPRGSSFSSSSSSMSLMKSTRSSLHSVCSSCSEGNTKQVSRKPSTLGCFPMH 177
SS S S+ S SS SSSSS + S S S SSC + PS + P+
Sbjct: 1650 SSSSSSIQSSSISSSSSSSSLIKSSASDHSCSSSPLSSCLFSISFSPIPSPSXVRPAPLP 1471
Query: 178 ISRVLVSITRR 188
+ + +S R
Sbjct: 1470 LGKRGISTNER 1438
>TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding protein -
Arabidopsis thaliana, partial (74%)
Length = 1909
Score = 32.7 bits (73), Expect = 0.086
Identities = 23/58 (39%), Positives = 31/58 (52%), Gaps = 3/58 (5%)
Frame = -2
Query: 118 SSDSESMVSPRGSSFSSSSSSMSLMKSTRSSLHSVCSSCSEGNTKQVSR---KPSTLG 172
SS S+ ++S SS SSSSSS S ST +S +S SS + SR P ++G
Sbjct: 387 SSSSKGLLSSSTSSSSSSSSSSSTSSSTSTSSYSTSSSLDSS*SLSPSRSTCSPESVG 214
Score = 28.1 bits (61), Expect = 2.1
Identities = 24/61 (39%), Positives = 27/61 (43%), Gaps = 2/61 (3%)
Frame = -2
Query: 117 CSSDSESMVSPRG--SSFSSSSSSMSLMKSTRSSLHSVCSSCSEGNTKQVSRKPSTLGCF 174
C S S S +G SS +SSSSS S ST SS + S S S PS C
Sbjct: 408 CFFLSASSSSSKGLLSSSTSSSSSSSSSSSTSSSTSTSSYSTSSSLDSS*SLSPSRSTCS 229
Query: 175 P 175
P
Sbjct: 228 P 226
>TC85722 similar to SP|P08283|H1_PEA Histone H1. [Garden pea] {Pisum
sativum}, partial (61%)
Length = 897
Score = 32.3 bits (72), Expect = 0.11
Identities = 29/133 (21%), Positives = 55/133 (40%)
Frame = +3
Query: 43 IAKESSPKEVLPPLTPPPKNLSLGLPKPSILELKKPASTMSKLKFWKKRVVKIKSKKPQE 102
+AKES PK+V P P + K +I+ LK+ + + + K +K ++
Sbjct: 195 VAKESKPKKVSKPRNPASHPTYEEMIKDAIVSLKERTGSS------QYAIAKFIEEKHKQ 356
Query: 103 VCFHEDFDILSRLDCSSDSESMVSPRGSSFSSSSSSMSLMKSTRSSLHSVCSSCSEGNTK 162
+ + +L L + S +V +GS SS++ + + + + + TK
Sbjct: 357 LPSNFKKLLLQNLKKNVASGKLVKVKGSFKLSSATKPAAKVKAKPAAKPKAKAVVKPRTK 536
Query: 163 QVSRKPSTLGCFP 175
V+ KP P
Sbjct: 537 SVTAKPKAAAAKP 575
>TC92834 similar to GP|16209669|gb|AAL14393.1 AT4g02500/T10P11_20
{Arabidopsis thaliana}, partial (46%)
Length = 910
Score = 32.3 bits (72), Expect = 0.11
Identities = 19/62 (30%), Positives = 32/62 (50%)
Frame = +2
Query: 104 CFHEDFDILSRLDCSSDSESMVSPRGSSFSSSSSSMSLMKSTRSSLHSVCSSCSEGNTKQ 163
C+ D+ + CS+ S + +SP S S SSSS++ + S L + S+ S N+
Sbjct: 125 CWSGVSDLAVSVRCSAHSAAALSPSSVSSSPSSSSVAQLAPANSELQNKTSTRSVNNSTH 304
Query: 164 VS 165
V+
Sbjct: 305 VA 310
>TC90105 weakly similar to GP|10241636|emb|CAC09475. putative zinc finger
protein {Oryza sativa}, partial (21%)
Length = 653
Score = 31.2 bits (69), Expect = 0.25
Identities = 21/62 (33%), Positives = 27/62 (42%), Gaps = 2/62 (3%)
Frame = +1
Query: 41 PGIAKESSPKEVLPPLTPPPKNLS--LGLPKPSILELKKPASTMSKLKFWKKRVVKIKSK 98
P +SSP +P TPPP L+ P PSIL P+ WK + K+ S
Sbjct: 223 PPSPTKSSPSSTIP*STPPPSFLASPSSPPPPSILSTTTPSPP------WKIPIAKLWSS 384
Query: 99 KP 100
P
Sbjct: 385 TP 390
>TC87119 similar to GP|20197209|gb|AAM14973.1 expressed protein {Arabidopsis
thaliana}, complete
Length = 1424
Score = 31.2 bits (69), Expect = 0.25
Identities = 37/111 (33%), Positives = 53/111 (47%), Gaps = 6/111 (5%)
Frame = -1
Query: 67 LPKPSILE--LKKPASTMSKLKFWKKRVVKIKSKKPQEVCFHEDFDILSRLDCSSDSESM 124
+P+P IL + PAS++SK +K+P V F+ L+ L+ S+
Sbjct: 794 VPRPYILSGGIVIPASSLSKAS----------AKRPCFVVTMYTFE-LANLNASATPG*N 648
Query: 125 VSP---RGSSFSSSSSSMSLMKSTRSSLHSVCSSCSEG-NTKQVSRKPSTL 171
+ P SFS+ SS +S RSSL S SS + G N Q S PST+
Sbjct: 647 LFP*IQPAKSFSNQSSLTFPNRSIRSSLFSFHSSMTIGFNLNQSSNMPSTV 495
>TC85721 similar to SP|P08283|H1_PEA Histone H1. [Garden pea] {Pisum
sativum}, partial (60%)
Length = 995
Score = 31.2 bits (69), Expect = 0.25
Identities = 28/126 (22%), Positives = 54/126 (42%)
Frame = +1
Query: 43 IAKESSPKEVLPPLTPPPKNLSLGLPKPSILELKKPASTMSKLKFWKKRVVKIKSKKPQE 102
+AKES PK+V P P + K +I+ LK+ + + + K +K ++
Sbjct: 229 VAKESKPKKVSKPRNPASHPTYEEMIKDAIVSLKERTGSS------QYAIAKFIEEKHKQ 390
Query: 103 VCFHEDFDILSRLDCSSDSESMVSPRGSSFSSSSSSMSLMKSTRSSLHSVCSSCSEGNTK 162
+ + +L L + S +V +GS SS++ + + + + + TK
Sbjct: 391 LPSNFKKLLLQNLKKNVASGKLVKVKGSFKLSSATKPAAKVKAKPAAKPKAKAVVKPKTK 570
Query: 163 QVSRKP 168
V+ KP
Sbjct: 571 SVTAKP 588
>BQ752445 similar to GP|18376387|em putative protein {Neurospora crassa},
partial (40%)
Length = 693
Score = 31.2 bits (69), Expect = 0.25
Identities = 22/51 (43%), Positives = 27/51 (52%)
Frame = +3
Query: 118 SSDSESMVSPRGSSFSSSSSSMSLMKSTRSSLHSVCSSCSEGNTKQVSRKP 168
SS S S S + SS SSS S S S+RSS HS S G+ +R+P
Sbjct: 543 SSSSSSKSSSKPSSKSSSKSHSSSHSSSRSSRHS--SRTRTGSRAATARRP 689
>TC84050 similar to GP|6273383|gb|AAF06346.1| SCUTL1 {Vitis vinifera},
partial (49%)
Length = 659
Score = 30.8 bits (68), Expect = 0.33
Identities = 23/85 (27%), Positives = 33/85 (38%), Gaps = 8/85 (9%)
Frame = -1
Query: 33 VPFKWEMQPGIAKESSPKEVLPPLTPPPKNLSLGLPKPSILELKKPASTMSKLKFWKKRV 92
V FK QP + P + PPL PP +S+G+ PS +S + K V
Sbjct: 650 VAFKSTGQPPLRSTRQPVGLQPPLVPPFATISIGIL*PSTKLTS*KSSPSAPFKVNSANV 471
Query: 93 --------VKIKSKKPQEVCFHEDF 109
+ +PQ HE+F
Sbjct: 470 AGGLAPPPAQATLPEPQSAVIHENF 396
>TC93070 weakly similar to GP|17065230|gb|AAL32769.1 Unknown protein
{Arabidopsis thaliana}, partial (19%)
Length = 679
Score = 30.8 bits (68), Expect = 0.33
Identities = 20/41 (48%), Positives = 23/41 (55%)
Frame = -3
Query: 119 SDSESMVSPRGSSFSSSSSSMSLMKSTRSSLHSVCSSCSEG 159
S S S S SS SSSSSS S S+ S+ S+ SS S G
Sbjct: 335 SSSSSSSSSSSSSSSSSSSSSSSSSSSSKSISSISSSESYG 213
Score = 27.3 bits (59), Expect = 3.6
Identities = 22/56 (39%), Positives = 27/56 (47%), Gaps = 2/56 (3%)
Frame = -3
Query: 108 DFDILSRL--DCSSDSESMVSPRGSSFSSSSSSMSLMKSTRSSLHSVCSSCSEGNT 161
DF++LS S + S S SS SSSSSS S S+ SS SS S +
Sbjct: 386 DFNLLSLALRSVGSANPSSSSSSSSSSSSSSSSSSSSSSSSSSSSKSISSISSSES 219
>TC89697 similar to GP|4886756|gb|AAD32031.1| cellulose synthase catalytic
subunit {Arabidopsis thaliana}, partial (26%)
Length = 1011
Score = 30.4 bits (67), Expect = 0.43
Identities = 29/74 (39%), Positives = 37/74 (49%), Gaps = 5/74 (6%)
Frame = -1
Query: 104 CFHED-FDILSRLDCSSDSESMVSPRGSS-FSSSSSSMSLMKSTRSSLH---SVCSSCSE 158
CFH F +LS S + RG S + SSSSM + S SL+ S+ +SCS
Sbjct: 498 CFHSSVFSLLSNF-AP*FSPIL*EYRGCSLYFVSSSSMLFILS*CISLYFSSSILNSCSI 322
Query: 159 GNTKQVSRKPSTLG 172
+T S PSTLG
Sbjct: 321 SSTSSSSSSPSTLG 280
>AW774261 weakly similar to EGAD|146423|156 vitellogenin {Anolis pulchellus},
partial (40%)
Length = 495
Score = 30.0 bits (66), Expect = 0.56
Identities = 25/70 (35%), Positives = 37/70 (52%), Gaps = 7/70 (10%)
Frame = -2
Query: 109 FDILSRLDCSSDS---ESMVSPRGSSFSSSSSSMSLMKSTRS----SLHSVCSSCSEGNT 161
F ++S SS S S S SSFSS+SSS+S S+ S S+ S+ SS S ++
Sbjct: 476 FCMVSSSSTSSSSFSYSSSSSSASSSFSSTSSSISSTSSSSSTSSYSISSISSSGSFSSS 297
Query: 162 KQVSRKPSTL 171
+ + ST+
Sbjct: 296 RSIFSSDSTV 267
>BQ137953 weakly similar to GP|22136724|gb unknown protein {Arabidopsis
thaliana}, partial (21%)
Length = 657
Score = 29.6 bits (65), Expect = 0.73
Identities = 21/61 (34%), Positives = 27/61 (43%)
Frame = -1
Query: 113 SRLDCSSDSESMVSPRGSSFSSSSSSMSLMKSTRSSLHSVCSSCSEGNTKQVSRKPSTLG 172
S LDCSS S S G FSSS S + L+ S + S S +R+ T+
Sbjct: 294 SSLDCSSSSSFSASSLGFIFSSSWSFIFLLSSR*EVVGGGALSSSSSLVVATNRRRVTIV 115
Query: 173 C 173
C
Sbjct: 114 C 112
>BG644352 homologue to GP|12231300|gb ripening regulated protein DDTFR10
{Lycopersicon esculentum}, partial (98%)
Length = 726
Score = 29.6 bits (65), Expect = 0.73
Identities = 27/86 (31%), Positives = 41/86 (47%), Gaps = 3/86 (3%)
Frame = -3
Query: 56 LTPPPKNLSLGLP--KPSILELKKPASTMSKLKFWKKRVVKIKSKK-PQEVCFHEDFDIL 112
L P P+ SL P PSI L++ +S+ + ++++ P F E F
Sbjct: 667 LIPYPQGTSLDAP*VNPSI*TLRQLSSSFFISVSSSHGLTSMRTEDLPDSFFFPEAFIAA 488
Query: 113 SRLDCSSDSESMVSPRGSSFSSSSSS 138
+R + S S+ SP S+ SSSSSS
Sbjct: 487 ARSSAAFFSSSVSSPNRSTSSSSSSS 410
>TC88868
Length = 742
Score = 29.6 bits (65), Expect = 0.73
Identities = 36/153 (23%), Positives = 59/153 (38%), Gaps = 14/153 (9%)
Frame = +2
Query: 33 VPFKWEMQPGIAKES----------SPKEVLPPLTPPPKNLSLG--LPKPSILELKKPAS 80
+ F WE +PGI KES E LP L PP + G P+ + + + P
Sbjct: 107 ISFSWEKKPGIPKESVNTNGENLIQRENEFLPKLPLPPISSLEGATTPRKLVHDFQIPLP 286
Query: 81 TMSKLKFWKKRVVKIKSKKPQEVCFHEDFDILSRLDCSSDSESMVSPRGSSFSSSSSSMS 140
+ ++ + SKK V + F + + +C+ +S + S SSS
Sbjct: 287 PCA----FQPPYYRTSSKKGIWVQDDDPF-LAAYKECTKSQKSGKRDKKMSRPGSSSFCE 451
Query: 141 LMKSTRSSLHSVCSSCS--EGNTKQVSRKPSTL 171
S S SC+ + N ++S P +
Sbjct: 452 SRLVKNMSFFSCKRSCNVRDNNLVRISHSPQNI 550
>BF638600 similar to SP|O14405|GUN4_ Endoglucanase IV precursor (EC 3.2.1.4)
(Endo-1 4-beta-glucanase IV) (Cellulase IV) (EGIV).,
partial (10%)
Length = 690
Score = 29.6 bits (65), Expect = 0.73
Identities = 40/169 (23%), Positives = 73/169 (42%), Gaps = 4/169 (2%)
Frame = +3
Query: 24 ISYYRSGEGVPFKWEMQPGIAK--ESSPKEVLPPLTPPPKNLSLGLPKPSILELKKPAST 81
+ YY S + + QP ++ S P+ PPL P L PS L P++T
Sbjct: 102 VGYYHSLQTTRPTFLTQPPFSRCLLSPPRLFSPPLPVPWALLPTVTFPPSTL--LAPSTT 275
Query: 82 MSKLKFWKKRVVKIKSKKPQEVCFHEDFDILSRLDCSSDSESMVSPRGSSFSSSSSSMSL 141
+S L ++ ++ +P S L ++ SP S ++SS+ ++
Sbjct: 276 VSMLAL----LLTTRTPRP------------SSLGPPPLPTTVSSPLMPSAPATSSATAM 407
Query: 142 MKSTRSSLHS--VCSSCSEGNTKQVSRKPSTLGCFPMHISRVLVSITRR 188
+++ R + S V S S G Q + + +L C+P+ + + +TRR
Sbjct: 408 LRTPRVTRRSRLVIRSISSGIPGQRATRAPSLTCWPLAVLPAVSPLTRR 554
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.313 0.128 0.368
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,411,165
Number of Sequences: 36976
Number of extensions: 133496
Number of successful extensions: 1848
Number of sequences better than 10.0: 131
Number of HSP's better than 10.0 without gapping: 1346
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1588
length of query: 189
length of database: 9,014,727
effective HSP length: 91
effective length of query: 98
effective length of database: 5,649,911
effective search space: 553691278
effective search space used: 553691278
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC135801.7