BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135799.5 + phase: 0
(171 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC90756 similar to PIR|T41394|T41394 hypothetical serine-rich pr... 60 4e-16
TC91093 weakly similar to PIR|T01873|T01873 hypothetical protein... 57 3e-09
BF649736 36 0.007
BG644643 similar to PIR|H86440|H864 unknown protein [imported] -... 33 0.043
TC79242 29 1.1
TC91065 similar to GP|22022579|gb|AAM83246.1 AT3g05090/T12H1_5 {... 27 3.1
TC82226 similar to PIR|T48615|T48615 hypothetical protein F18O22... 27 4.0
TC76491 similar to SP|O65781|GAE2_CYATE UDP-glucose 4-epimerase ... 27 4.0
BF645678 27 4.0
TC79090 similar to PIR|T46111|T46111 probable transposase - Arab... 27 5.3
AJ501083 26 6.9
TC82926 26 9.0
>TC90756 similar to PIR|T41394|T41394 hypothetical serine-rich protein -
fission yeast (Schizosaccharomyces pombe), partial (7%)
Length = 1430
Score = 59.7 bits (143), Expect(2) = 4e-16
Identities = 31/73 (42%), Positives = 48/73 (65%)
Frame = -3
Query: 99 LSMMHNSTFTKVHLPAIPMNVFEFKPFNEILSSTVEEVSTDVIGHVIERGDIRETEKDRR 158
L+++ +T+TKV IP N F+F FN+++ E + GHV+E+ DIR+ E + R
Sbjct: 1014 LNLID*TTWTKVD*NVIPNNHFDFMAFNDVMQ*GNEGQYIYINGHVVEKDDIRDKEVNGR 835
Query: 159 KSRVIDLTLEDLE 171
S++ID+TLEDLE
Sbjct: 834 TSKLIDITLEDLE 796
Score = 40.8 bits (94), Expect(2) = 4e-16
Identities = 20/48 (41%), Positives = 30/48 (61%), Gaps = 1/48 (2%)
Frame = -1
Query: 53 RIHATTRKDLVAKFRSMVQEGGTYQLENAIVDFNESPYKVTS-HKHKL 99
+I AT ++LVAK +S V+EG Y++EN + N+ Y HK+KL
Sbjct: 1160 KIQATVERNLVAKIKSEVEEGQAYEVENVLFTHNDPKYVYYKVHKYKL 1017
>TC91093 weakly similar to PIR|T01873|T01873 hypothetical protein T24M8.10 -
Arabidopsis thaliana, partial (11%)
Length = 701
Score = 57.4 bits (137), Expect = 3e-09
Identities = 35/114 (30%), Positives = 57/114 (49%)
Frame = +3
Query: 22 VRVAHIWLIREKKVPTSIIFMNMLLVDEKGGRIHATTRKDLVAKFRSMVQEGGTYQLENA 81
VR+ +W++ + + M+L D KG +I KD +AK+ S++ E TY ++N
Sbjct: 18 VRITDMWIVTNMN---NHQHLEMVLTDSKGDKIQGIIVKDDIAKWGSILIEQKTYVMQNF 188
Query: 82 IVDFNESPYKVTSHKHKLSMMHNSTFTKVHLPAIPMNVFEFKPFNEILSSTVEE 135
V N+ +K+ H K+ + S + P IP EFK F EIL+ + E
Sbjct: 189 KVLKNDLQFKLCDHPCKMIIHGGSVICEHPFPNIPRTKVEFKTFAEILTGKMSE 350
>BF649736
Length = 274
Score = 36.2 bits (82), Expect = 0.007
Identities = 20/54 (37%), Positives = 27/54 (49%)
Frame = +3
Query: 1 MSAKYTPISAVSGGRKNLKMCVRVAHIWLIREKKVPTSIIFMNMLLVDEKGGRI 54
MS T + V R + VRV +W I V +IFM M+L+D+ G RI
Sbjct: 108 MSPPITSLLNVRPPRMAWNIRVRVVRLWXIMSSLVRGXVIFMEMVLLDQDGNRI 269
>BG644643 similar to PIR|H86440|H864 unknown protein [imported] - Arabidopsis
thaliana, partial (3%)
Length = 758
Score = 33.5 bits (75), Expect = 0.043
Identities = 12/28 (42%), Positives = 20/28 (70%)
Frame = +3
Query: 1 MSAKYTPISAVSGGRKNLKMCVRVAHIW 28
M+ TP++A+ G+ N+K+ VRV H+W
Sbjct: 675 MAPLITPVAAIVAGKINIKLRVRVVHVW 758
>TC79242
Length = 1802
Score = 28.9 bits (63), Expect = 1.1
Identities = 15/58 (25%), Positives = 28/58 (47%)
Frame = +2
Query: 39 IIFMNMLLVDEKGGRIHATTRKDLVAKFRSMVQEGGTYQLENAIVDFNESPYKVTSHK 96
+I +++V++ + HA +R + R+ G FNE+P+K TSH+
Sbjct: 293 VIMETLVVVEQHKNQDHAPSRDFIGINCRTFESGYGVLPTPLRSQSFNENPHKKTSHR 466
>TC91065 similar to GP|22022579|gb|AAM83246.1 AT3g05090/T12H1_5 {Arabidopsis
thaliana}, partial (20%)
Length = 848
Score = 27.3 bits (59), Expect = 3.1
Identities = 11/44 (25%), Positives = 27/44 (61%)
Frame = -3
Query: 77 QLENAIVDFNESPYKVTSHKHKLSMMHNSTFTKVHLPAIPMNVF 120
++++AI ++P+ +TSH+ ++ F+ ++ PA+ +N F
Sbjct: 636 RVQDAIPPSTKAPFAITSHELLCNVGLGHLFSSIYKPALWLNSF 505
>TC82226 similar to PIR|T48615|T48615 hypothetical protein F18O22.210 -
Arabidopsis thaliana, partial (63%)
Length = 1300
Score = 26.9 bits (58), Expect = 4.0
Identities = 29/107 (27%), Positives = 45/107 (41%), Gaps = 8/107 (7%)
Frame = +2
Query: 36 PTS---IIFMNMLLVDEKGGRIHATTRKDLVAKFRSMVQEGGTYQLE-----NAIVDFNE 87
PTS II M M +V++ GG+ H + RS+ E G + +AIV +E
Sbjct: 386 PTSFAPIIEMGMTIVEKSGGQYHVSLIIADGQVTRSVDTENGKLSSQEKKTIDAIVKASE 565
Query: 88 SPYKVTSHKHKLSMMHNSTFTKVHLPAIPMNVFEFKPFNEILSSTVE 134
P + ++PA + F+F F EI+S V+
Sbjct: 566 YPLSIVLVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTEIMSRNVD 706
>TC76491 similar to SP|O65781|GAE2_CYATE UDP-glucose 4-epimerase GEPI48 (EC
5.1.3.2) (Galactowaldenase) (UDP- galactose
4-epimerase)., partial (96%)
Length = 1805
Score = 26.9 bits (58), Expect = 4.0
Identities = 11/24 (45%), Positives = 16/24 (65%)
Frame = -3
Query: 13 GGRKNLKMCVRVAHIWLIREKKVP 36
GGRKNL C VA ++L++ + P
Sbjct: 495 GGRKNLLKCSLVAEVYLVKIEVFP 424
>BF645678
Length = 463
Score = 26.9 bits (58), Expect = 4.0
Identities = 11/23 (47%), Positives = 16/23 (68%)
Frame = +2
Query: 149 DIRETEKDRRKSRVIDLTLEDLE 171
D+R EKD R+ R +DL E++E
Sbjct: 56 DLRRREKDNRRMRTMDLFGEEME 124
>TC79090 similar to PIR|T46111|T46111 probable transposase - Arabidopsis
thaliana, partial (28%)
Length = 1068
Score = 26.6 bits (57), Expect = 5.3
Identities = 22/105 (20%), Positives = 49/105 (45%), Gaps = 7/105 (6%)
Frame = +2
Query: 73 GGTYQLENAIVDFNESPYKVTSHKHKLSMMHNSTFTKVHLPAIP-MNVFEFKPFNEILSS 131
GGT +N + DF+ + ++H+ K + + + LP +P +V + N++
Sbjct: 500 GGTLLSDNGLTDFDAYIMETSTHQTKSEL--DQYLEESLLPRVPDFDVLGWWKLNKLKYP 673
Query: 132 TVEEVSTDVIGHVIER------GDIRETEKDRRKSRVIDLTLEDL 170
T+ +++ D++ + D + E D+ +S + T+E L
Sbjct: 674 TLSKMARDILSIPVSTVPSDSIFDKKSKEMDQYRSSLRPETVEAL 808
>AJ501083
Length = 679
Score = 26.2 bits (56), Expect = 6.9
Identities = 14/47 (29%), Positives = 25/47 (52%)
Frame = -1
Query: 2 SAKYTPISAVSGGRKNLKMCVRVAHIWLIREKKVPTSIIFMNMLLVD 48
S K+ IS VS +++ + VRV W +++ K M ++L+D
Sbjct: 616 STKHDFISHVSPRKQSWTLXVRVIRAWFVQDYKNKKLSFSMELVLMD 476
>TC82926
Length = 820
Score = 25.8 bits (55), Expect = 9.0
Identities = 16/67 (23%), Positives = 26/67 (37%)
Frame = +2
Query: 79 ENAIVDFNESPYKVTSHKHKLSMMHNSTFTKVHLPAIPMNVFEFKPFNEILSSTVEEVST 138
+N FNE Y SH H L H + + + N K F + + + T
Sbjct: 632 DNTFKGFNELQYVTFSHLHTLEFHHGCPKVDILIKLLESNGKNLKTF----VAEIPDKYT 799
Query: 139 DVIGHVI 145
D++ + I
Sbjct: 800 DLLNNAI 820
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.134 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,407,791
Number of Sequences: 36976
Number of extensions: 53071
Number of successful extensions: 316
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 316
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 316
length of query: 171
length of database: 9,014,727
effective HSP length: 89
effective length of query: 82
effective length of database: 5,723,863
effective search space: 469356766
effective search space used: 469356766
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC135799.5