BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135797.3 - phase: 0 /pseudo
(460 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86820 similar to GP|15146218|gb|AAK83592.1 AT3g09470/F11F8_4 {... 327 e-162
TC86819 similar to GP|15146218|gb|AAK83592.1 AT3g09470/F11F8_4 {... 521 e-148
AW256515 similar to GP|15146218|gb AT3g09470/F11F8_4 {Arabidopsi... 151 4e-37
AW691583 similar to PIR|G84688|G846 hypothetical protein At2g287... 39 0.003
TC83721 similar to GP|9758335|dbj|BAB08891.1 gene_id:MIJ24.9~unk... 30 1.5
TC91568 similar to PIR|T00925|T00925 hypothetical protein At2g42... 30 2.0
TC88402 similar to GP|20804521|dbj|BAB92215. putative CRK1 prote... 30 2.0
TC79354 weakly similar to GP|15215800|gb|AAK91445.1 AT5g53860/K1... 30 2.6
BI271948 similar to GP|6498428|dbj Similar to Arabidopsis thalia... 30 2.6
TC91001 30 2.6
TC79734 similar to GP|15293215|gb|AAK93718.1 putative oligopepti... 29 4.4
TC90605 similar to GP|22136848|gb|AAM91768.1 unknown protein {Ar... 29 4.4
TC77789 weakly similar to GP|6671937|gb|AAF23197.1| unknown prot... 28 5.7
BG645703 similar to PIR|T05354|T053 hypothetical protein F8B4.14... 28 9.7
TC78105 homologue to GP|19568098|gb|AAL89456.1 osmotic stress-ac... 28 9.7
TC78750 weakly similar to GP|16519227|gb|AAL25130.1 cellulose sy... 28 9.7
>TC86820 similar to GP|15146218|gb|AAK83592.1 AT3g09470/F11F8_4 {Arabidopsis
thaliana}, partial (75%)
Length = 1646
Score = 327 bits (838), Expect(3) = e-162
Identities = 188/301 (62%), Positives = 205/301 (67%)
Frame = +2
Query: 1 MASVIIHRDEETPLVVATDESPIQFQQHKSHTRDVHILSIAFLLIFLAYGAAQNLQSTLN 60
MASV IH DEETPLVV ESPIQ Q HKSH+RDVHILSIAF+LIFLAYGAAQNLQSTLN
Sbjct: 152 MASVTIHHDEETPLVV---ESPIQLQPHKSHSRDVHILSIAFVLIFLAYGAAQNLQSTLN 322
Query: 61 TASGELIVCIEFSGGGFGYDFAWDIIFVFYIFFCVCFFGGADSRIQECFDCWNFGLLVIP 120
T + G + F + V G ++ I W + +
Sbjct: 323 TEE-------DLGTTSLGILYLSFTFFSVFASLVVRILGSKNALIIGTSGYWLYVAANLK 481
Query: 121 GCKFEAQLVYVGSGFCLSWVLRFYIMGWAACQGTYLTSTARSHAIDNNFHEGAVIGDFNG 180
+ V GFC S + W QGTYLTSTA SHAIDNN HEGAVIGDFNG
Sbjct: 482 PNWYTLVPASVYLGFCASII-------WVG-QGTYLTSTAHSHAIDNNLHEGAVIGDFNG 637
Query: 181 EFWGVYTLHQFIGNLITFALLSDGQEGSTNGTTLLFVVFLSVMTFGAILTCFLHKRGDYS 240
EFW VY LHQFIGNLITFALLSDGQ GSTNGTTLLFVVFL VMTFGAIL FLHKR S
Sbjct: 638 EFWAVYALHQFIGNLITFALLSDGQGGSTNGTTLLFVVFLFVMTFGAILMFFLHKRIGNS 817
Query: 241 KGGYKHLDAGTGQSKSLKSLCRSLTGALSDVKMLLIIPLIAYSGLQHAFVWAEFTKYVVT 300
K YK+ +A +SKSLKSLC+SLT ALSD KMLLIIPL+AYSGLQ AFVWAEFTK+VVT
Sbjct: 818 KEEYKNSEADVVESKSLKSLCKSLTSALSDAKMLLIIPLMAYSGLQQAFVWAEFTKFVVT 997
Query: 301 P 301
P
Sbjct: 998 P 1000
Score = 232 bits (592), Expect(3) = e-162
Identities = 119/132 (90%), Positives = 126/132 (95%)
Frame = +1
Query: 329 LTFGLTSITSIVSFGAFVQAVVLILLLLDFSMSSGFIGTLYILFLAALLGIGDGVLMTQL 388
LT GLTSITSIVSFGAF QAV LILLLL+FS+SSGF+GT YILFLAALLGIGDGVLMTQL
Sbjct: 1084 LTSGLTSITSIVSFGAFAQAVALILLLLNFSISSGFLGTSYILFLAALLGIGDGVLMTQL 1263
Query: 389 NALLGMLFKHDMEGAFAQLKIWQSATIAMVFFLAPYISFQAVIMVMLTLLCLSFCSFLWL 448
NALLG+LFKHDMEGAFAQLKIWQSATIA+VFFLAPYISFQAV ++ML LLCLSFCSFLWL
Sbjct: 1264 NALLGILFKHDMEGAFAQLKIWQSATIAVVFFLAPYISFQAVTIIMLVLLCLSFCSFLWL 1443
Query: 449 ALKVGNASSPST 460
ALKVGNASSPST
Sbjct: 1444 ALKVGNASSPST 1479
Score = 51.6 bits (122), Expect(3) = e-162
Identities = 24/26 (92%), Positives = 24/26 (92%)
Frame = +3
Query: 303 IGVSGVGIAMAVYGAFDGICSLVAGR 328
IGVSGVGIAMA YGAFDGICSL AGR
Sbjct: 1005 IGVSGVGIAMAAYGAFDGICSLAAGR 1082
>TC86819 similar to GP|15146218|gb|AAK83592.1 AT3g09470/F11F8_4 {Arabidopsis
thaliana}, partial (60%)
Length = 1234
Score = 521 bits (1342), Expect = e-148
Identities = 283/362 (78%), Positives = 292/362 (80%)
Frame = +1
Query: 1 MASVIIHRDEETPLVVATDESPIQFQQHKSHTRDVHILSIAFLLIFLAYGAAQNLQSTLN 60
MASVIIHRDEETPLVVATDESPIQFQQHKSHTRDVHILSIAFLLIFLAYGAAQNLQSTLN
Sbjct: 193 MASVIIHRDEETPLVVATDESPIQFQQHKSHTRDVHILSIAFLLIFLAYGAAQNLQSTLN 372
Query: 61 TASGELIVCIEFSGGGFGYDFAWDIIFVFYIFFCVCFFGGADSRIQECFDCWNFGLLVIP 120
T + G + F + V G ++ I W + +
Sbjct: 373 TEE-------DLGTTSLGILYLSFTFFSVFASLVVRILGSKNALIVGTSGYWLYLAANLK 531
Query: 121 GCKFEAQLVYVGSGFCLSWVLRFYIMGWAACQGTYLTSTARSHAIDNNFHEGAVIGDFNG 180
+ V GFC S + W QGTYLTSTARSHAIDNNFHEGAVIGDFNG
Sbjct: 532 PNWYTLVPASVYLGFCASIL-------WVG-QGTYLTSTARSHAIDNNFHEGAVIGDFNG 687
Query: 181 EFWGVYTLHQFIGNLITFALLSDGQEGSTNGTTLLFVVFLSVMTFGAILTCFLHKRGDYS 240
EFWGVYTLHQFIGNLITFALLSDGQEGSTNGTTLLFVVFLSVMTFGAILTCFLHKRGDYS
Sbjct: 688 EFWGVYTLHQFIGNLITFALLSDGQEGSTNGTTLLFVVFLSVMTFGAILTCFLHKRGDYS 867
Query: 241 KGGYKHLDAGTGQSKSLKSLCRSLTGALSDVKMLLIIPLIAYSGLQHAFVWAEFTKYVVT 300
KGGYKHLDAGTGQSKSLKSLCRSLTGALSDVKMLLIIPLIAYSGLQHAFVWAEFTKYVVT
Sbjct: 868 KGGYKHLDAGTGQSKSLKSLCRSLTGALSDVKMLLIIPLIAYSGLQHAFVWAEFTKYVVT 1047
Query: 301 PEIGVSGVGIAMAVYGAFDGICSLVAGRLTFGLTSITSIVSFGAFVQAVVLILLLLDFSM 360
PEIGVSGVGIAMAVYGAFDGICSLVAGRLTFGLTSITSIVSFGAFV AVVLILLLLDFSM
Sbjct: 1048PEIGVSGVGIAMAVYGAFDGICSLVAGRLTFGLTSITSIVSFGAFVHAVVLILLLLDFSM 1227
Query: 361 SS 362
SS
Sbjct: 1228SS 1233
>AW256515 similar to GP|15146218|gb AT3g09470/F11F8_4 {Arabidopsis thaliana},
partial (25%)
Length = 624
Score = 151 bits (382), Expect = 4e-37
Identities = 88/152 (57%), Positives = 96/152 (62%), Gaps = 3/152 (1%)
Frame = +1
Query: 1 MASVIIHRDEETPLVVATDESPIQFQQHKSHTRDVHILSIAFLLIFLAYGAAQNLQSTLN 60
MAS IIHRDEETPLVVATDESPIQFQQHKSHTRDVHILSIAFLLIFLAYGAAQNLQSTLN
Sbjct: 139 MASEIIHRDEETPLVVATDESPIQFQQHKSHTRDVHILSIAFLLIFLAYGAAQNLQSTLN 318
Query: 61 TASGELIVCIEFSGGGFGYDFAWDIIFVFYIFFCVCFFGGADSRIQECFDCWNFGLLVIP 120
T + G + F + V G ++ I W + +
Sbjct: 319 TEE-------DLGTTSLGILYLSFTFFSVFASLVVRILGSKNALIVGTSGYWLYLAANLK 477
Query: 121 G---CKFEAQLVYVGSGFCLSWVLRFYIMGWA 149
+ A VYVGSGFCLSWVLRFYIMGWA
Sbjct: 478 PNW*LQVHAIYVYVGSGFCLSWVLRFYIMGWA 573
Score = 37.7 bits (86), Expect = 0.009
Identities = 21/35 (60%), Positives = 22/35 (62%)
Frame = +2
Query: 134 GFCLSWVLRFYIMGWAACQGTYLTSTARSHAIDNN 168
GFC S + W QGTYLTSTAR HAIDNN
Sbjct: 542 GFCASIL-------WVG-QGTYLTSTARRHAIDNN 622
>AW691583 similar to PIR|G84688|G846 hypothetical protein At2g28780
[imported] - Arabidopsis thaliana, partial (10%)
Length = 433
Score = 39.3 bits (90), Expect = 0.003
Identities = 20/63 (31%), Positives = 37/63 (57%)
Frame = +1
Query: 322 CSLVAGRLTFGLTSITSIVSFGAFVQAVVLILLLLDFSMSSGFIGTLYILFLAALLGIGD 381
C++VAG +G SITS+++F AF VV+++++ D ++ G L+ A + +G
Sbjct: 118 CTIVAGATLYGPISITSLITFPAFSYVVVILIIINDATLGDSLRGCWLGLY-ATIQSLGP 294
Query: 382 GVL 384
+L
Sbjct: 295 AML 303
>TC83721 similar to GP|9758335|dbj|BAB08891.1 gene_id:MIJ24.9~unknown
protein {Arabidopsis thaliana}, partial (79%)
Length = 942
Score = 30.4 bits (67), Expect = 1.5
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +1
Query: 418 VFFLAPYISFQAVIMVMLTLLCLSFCSFL 446
VFFL+ +IS I+ ML LCLSF SF+
Sbjct: 757 VFFLS-FISLNKFIISMLCFLCLSFSSFI 840
>TC91568 similar to PIR|T00925|T00925 hypothetical protein At2g42150
[imported] - Arabidopsis thaliana, partial (2%)
Length = 932
Score = 30.0 bits (66), Expect = 2.0
Identities = 19/69 (27%), Positives = 31/69 (44%)
Frame = +3
Query: 235 KRGDYSKGGYKHLDAGTGQSKSLKSLCRSLTGALSDVKMLLIIPLIAYSGLQHAFVWAEF 294
KRG Y K KH+D T +S+ S D+ +L L+ YS + H + A
Sbjct: 96 KRGKYKKMIQKHMDFDTIRSRISSRTIESTRELYRDLLLLTNNALVFYSKITHEYKTALL 275
Query: 295 TKYVVTPEI 303
+ +V ++
Sbjct: 276 LRGIVAKKM 302
>TC88402 similar to GP|20804521|dbj|BAB92215. putative CRK1 protein {Oryza
sativa (japonica cultivar-group)}, partial (46%)
Length = 2184
Score = 30.0 bits (66), Expect = 2.0
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +3
Query: 418 VFFLAPYISFQAVIMVMLTLLCLSFCSFLWLALKVG 453
+FFL SF A I +++ CL F W+ L++G
Sbjct: 276 IFFLVKMFSFSARI*ILMFDCCLPASEFNWVGLEIG 383
>TC79354 weakly similar to GP|15215800|gb|AAK91445.1 AT5g53860/K19P17_2
{Arabidopsis thaliana}, partial (56%)
Length = 847
Score = 29.6 bits (65), Expect = 2.6
Identities = 19/55 (34%), Positives = 31/55 (55%)
Frame = +1
Query: 323 SLVAGRLTFGLTSITSIVSFGAFVQAVVLILLLLDFSMSSGFIGTLYILFLAALL 377
+L+A +L L S + S +Q ++L+LLL+ S F+ +L +LFL LL
Sbjct: 130 TLMAKQLAMSLLSPSKFSSLL*PLQLILLVLLLVALVFLSLFLSSLLLLFLRLLL 294
>BI271948 similar to GP|6498428|dbj Similar to Arabidopsis thaliana
chromosome II BAC T27A16 sequence; hypothetical protein.
(AC005496), partial (33%)
Length = 695
Score = 29.6 bits (65), Expect = 2.6
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +3
Query: 365 IGTLYILFLAALLGIGDGVLMTQLNALL 392
+G Y+L + A +GIG+GV M +N +L
Sbjct: 345 LGLPYLLVMRAFMGIGEGVAMPAMNNIL 428
>TC91001
Length = 808
Score = 29.6 bits (65), Expect = 2.6
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = -2
Query: 77 FGYDFAWDIIFVFYIFFCVCFFGGADSRIQECFDCWNFGL 116
FG + F+F+ FC+ F G + CF+CW+ GL
Sbjct: 420 FGPNLKTHSRFLFHRKFCLIFLGITGCTVN-CFNCWSKGL 304
>TC79734 similar to GP|15293215|gb|AAK93718.1 putative oligopeptide
transporter protein {Arabidopsis thaliana}, partial
(36%)
Length = 860
Score = 28.9 bits (63), Expect = 4.4
Identities = 20/75 (26%), Positives = 38/75 (50%)
Frame = +1
Query: 312 MAVYGAFDGICSLVAGRLTFGLTSITSIVSFGAFVQAVVLILLLLDFSMSSGFIGTLYIL 371
MA+ + + + + + F L +IV+ FV + LI LL + +S F+GT +
Sbjct: 253 MAIAAVGNNLITYLINEMHFSLPHSANIVT--NFVGTIFLIALLGGY-LSDSFLGTFSTI 423
Query: 372 FLAALLGIGDGVLMT 386
+ ALL + +L++
Sbjct: 424 LIFALLELSGFILLS 468
>TC90605 similar to GP|22136848|gb|AAM91768.1 unknown protein {Arabidopsis
thaliana}, partial (54%)
Length = 1156
Score = 28.9 bits (63), Expect = 4.4
Identities = 22/60 (36%), Positives = 30/60 (49%)
Frame = -1
Query: 85 IIFVFYIFFCVCFFGGADSRIQECFDCWNFGLLVIPGCKFEAQLVYVGSGFCLSWVLRFY 144
IIF+F++FF + C W F LV C + LV+VG GFC S+V F+
Sbjct: 256 IIFIFFLFF--------QCMTRRCLFLWFFVCLVSL-CFY---LVFVGGGFC-SFVTCFH 116
>TC77789 weakly similar to GP|6671937|gb|AAF23197.1| unknown protein
{Arabidopsis thaliana}, partial (32%)
Length = 1149
Score = 28.5 bits (62), Expect = 5.7
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 5/45 (11%)
Frame = +3
Query: 191 FIGNLITFALLSDGQEGSTNGTT-----LLFVVFLSVMTFGAILT 230
F+ L F + Q+ STN TT LLF++ ++ FGA++T
Sbjct: 159 FLSLLFQFISKNFQQQLSTNPTTIISLYLLFIILSCIIAFGAVIT 293
>BG645703 similar to PIR|T05354|T053 hypothetical protein F8B4.140 -
Arabidopsis thaliana, partial (13%)
Length = 822
Score = 27.7 bits (60), Expect = 9.7
Identities = 18/49 (36%), Positives = 26/49 (52%)
Frame = +2
Query: 39 SIAFLLIFLAYGAAQNLQSTLNTASGELIVCIEFSGGGFGYDFAWDIIF 87
SI+ + +FL + A L S+ T G+L C FSG G+G+ IF
Sbjct: 530 SISTIPVFLLFTEAC-LYSSAGTCLGKLTFCTCFSGVGWGFQLLLCNIF 673
>TC78105 homologue to GP|19568098|gb|AAL89456.1 osmotic stress-activated
protein kinase {Nicotiana tabacum}, partial (35%)
Length = 731
Score = 27.7 bits (60), Expect = 9.7
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = +1
Query: 85 IIFVFYIFFCVCFFG 99
II + Y+F C CFFG
Sbjct: 355 IIIIIYVFTCFCFFG 399
>TC78750 weakly similar to GP|16519227|gb|AAL25130.1 cellulose synthase-like
protein OsCslE2 {Oryza sativa}, partial (6%)
Length = 1044
Score = 27.7 bits (60), Expect = 9.7
Identities = 21/73 (28%), Positives = 35/73 (47%)
Frame = +1
Query: 380 GDGVLMTQLNALLGMLFKHDMEGAFAQLKIWQSATIAMVFFLAPYISFQAVIMVMLTLLC 439
GD + TQ + + + +M+ +QL W S + +F ++ Y F I + + C
Sbjct: 148 GDQPIFTQKYHVS*DVLRTNMKEGMSQLIKWVSELL--LFAISKYSPFTYGISRLPIVHC 321
Query: 440 LSFCSFLWLALKV 452
L+FC LAL V
Sbjct: 322 LTFCHLSSLALYV 360
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.329 0.143 0.445
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,064,716
Number of Sequences: 36976
Number of extensions: 258890
Number of successful extensions: 2193
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 2153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2185
length of query: 460
length of database: 9,014,727
effective HSP length: 99
effective length of query: 361
effective length of database: 5,354,103
effective search space: 1932831183
effective search space used: 1932831183
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC135797.3