BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135795.5 + phase: 0
(471 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78638 similar to GP|22087278|gb|AAM90909.1 p44/SSL1-like prote... 484 0.0
TC87551 similar to PIR|T12317|T12317 26S proteasome regulatory s... 38 0.010
TC93216 similar to PIR|T51606|T51606 probable 26S proteasome non... 36 0.036
TC88763 similar to GP|5091601|gb|AAD39590.1| This gene is a memb... 33 0.18
TC77535 similar to GP|17381208|gb|AAL36416.1 unknown protein {Ar... 31 1.2
TC77337 weakly similar to GP|4019275|gb|AAC95573.1| orf 48 {Atel... 30 2.0
TC80460 similar to GP|21537277|gb|AAM61618.1 unknown {Arabidopsi... 30 2.0
TC87964 weakly similar to PIR|G96757|G96757 probable protein ATP... 29 3.4
CB065276 similar to SP|Q37546|NU1M_ NADH-ubiquinone oxidoreducta... 28 5.8
TC88481 similar to PIR|T00806|T02445 probable U4/U6 small nuclea... 28 5.8
TC78348 weakly similar to GP|549986|gb|AAA80576.1|| possible apo... 28 5.8
TC77706 similar to GP|14532908|gb|AAK64136.1 unknown protein {Ar... 28 5.8
TC89427 weakly similar to GP|4557063|gb|AAD22502.1| expressed pr... 28 7.6
CA917961 homologue to GP|12325083|gb| hypothetical protein; 3218... 28 7.6
TC90331 weakly similar to GP|21751020|dbj|BAC03887. unnamed prot... 28 7.6
TC92413 weakly similar to GP|21618067|gb|AAM67117.1 unknown {Ara... 28 7.6
BQ147693 similar to GP|5668770|gb T17H3.9 {Arabidopsis thaliana}... 28 9.9
TC91460 similar to GP|19698839|gb|AAL91155.1 cytochrome P450 {Ar... 28 9.9
TC79422 similar to PIR|T47953|T47953 hypothetical protein F2A19.... 28 9.9
BF003643 weakly similar to PIR|F84686|F846 hypothetical protein ... 28 9.9
>TC78638 similar to GP|22087278|gb|AAM90909.1 p44/SSL1-like protein
{Arabidopsis thaliana}, partial (85%)
Length = 2060
Score = 484 bits (1247), Expect(2) = 0.0
Identities = 242/273 (88%), Positives = 246/273 (89%), Gaps = 1/273 (0%)
Frame = +2
Query: 128 SHVGLVTTKDGVANCL-TDLGGSPESHIKALMGKLECSGDASLQNALELVHSNLNQIPSY 186
S +GLVTTK+ V TDLGGSPESHIKALMGKLECSGDASLQNALELVHSNLNQIPSY
Sbjct: 725 SWLGLVTTKEWVLLIAWTDLGGSPESHIKALMGKLECSGDASLQNALELVHSNLNQIPSY 904
Query: 187 GHREVLILYSALSTCDPGDLMETIQKCKKSKIRCSVIGLAAEMFICKHLCQETGGTYSVA 246
GHREVLILYSALSTCDPGDLMETIQKCKKSKIRCSVIGLAAEMFICKHLCQE GGTYSVA
Sbjct: 905 GHREVLILYSALSTCDPGDLMETIQKCKKSKIRCSVIGLAAEMFICKHLCQEIGGTYSVA 1084
Query: 247 LDESHFKELILEHSPPPPAIAEYATANLIKMGFPQRAAEGSVAICTCHEEAKTGGGYTCP 306
LDESHFKELILEHSPPPPAIAEYATANLIKMGFPQRAAEGSVAICTCHEEAKTGGGYTCP
Sbjct: 1085LDESHFKELILEHSPPPPAIAEYATANLIKMGFPQRAAEGSVAICTCHEEAKTGGGYTCP 1264
Query: 307 RCKVRVCELPTECRVCGLTLISSPHLARSYHHLFPIVPFAEISPSSQNDPNHSFPNTCFG 366
RCKVRVCELPTECRVCGLTLISSPHLARSYHHLFPIVPFAEISPSSQNDPNHSFPNTCFG
Sbjct: 1265RCKVRVCELPTECRVCGLTLISSPHLARSYHHLFPIVPFAEISPSSQNDPNHSFPNTCFG 1444
Query: 367 CQQSLLVLEQETRLNFPSLAQNANNNSALIVTC 399
CQQSLL S A N + L V+C
Sbjct: 1445CQQSLL-----------SQGFGAGNKAELSVSC 1510
Score = 259 bits (662), Expect(2) = 0.0
Identities = 129/129 (100%), Positives = 129/129 (100%)
Frame = +3
Query: 1 MNNIAGKPLNGDLEDDDEDEANDDGLEAWERAYTEDRSWESLQEDESGLLRPIDTTAIHH 60
MNNIAGKPLNGDLEDDDEDEANDDGLEAWERAYTEDRSWESLQEDESGLLRPIDTTAIHH
Sbjct: 342 MNNIAGKPLNGDLEDDDEDEANDDGLEAWERAYTEDRSWESLQEDESGLLRPIDTTAIHH 521
Query: 61 AQYRRRLRALASNAATARIQKGLIRYLYIVVDLSKAASERDFRPSRMAVIAKQVELFIRE 120
AQYRRRLRALASNAATARIQKGLIRYLYIVVDLSKAASERDFRPSRMAVIAKQVELFIRE
Sbjct: 522 AQYRRRLRALASNAATARIQKGLIRYLYIVVDLSKAASERDFRPSRMAVIAKQVELFIRE 701
Query: 121 FFDQNPLSH 129
FFDQNPLSH
Sbjct: 702 FFDQNPLSH 728
Score = 204 bits (518), Expect = 8e-53
Identities = 110/143 (76%), Positives = 111/143 (76%), Gaps = 2/143 (1%)
Frame = +3
Query: 331 HLARSYHHLFPIVPFAEISPSSQNDPNHSFPNTCFGCQQSLLV--LEQETRLNFPSLAQN 388
HL RS HHL I FP + LV LEQETRLNFPSLAQN
Sbjct: 1377 HLLRSLHHLKMIQTIV-------------FPILVLVVNKVSLVRVLEQETRLNFPSLAQN 1517
Query: 389 ANNNSALIVTCTFMRVYIIAQAVRASGILSQLLLPNDFQPYALDIKLLSKWCSTLPPCYC 448
ANNNSALIVTCTFMRVYIIAQAVRASGILSQLLLPNDFQPYALDIKLLSKWCSTLPPCYC
Sbjct: 1518 ANNNSALIVTCTFMRVYIIAQAVRASGILSQLLLPNDFQPYALDIKLLSKWCSTLPPCYC 1697
Query: 449 DVASIYRTHEQVPVHEMELTNTI 471
DVASIYRTHEQVPVHEMELTNTI
Sbjct: 1698 DVASIYRTHEQVPVHEMELTNTI 1766
>TC87551 similar to PIR|T12317|T12317 26S proteasome regulatory subunit S5A
- common ice plant, partial (68%)
Length = 936
Score = 37.7 bits (86), Expect = 0.010
Identities = 43/176 (24%), Positives = 73/176 (41%), Gaps = 5/176 (2%)
Frame = +1
Query: 58 IHHAQYRRRLRALASNAATARIQKGLIRYLYIVVDLSKAASERDFRPSRMAVIAKQVELF 117
+HH + LAS ++T ++ +I +D S+ D+ PSR A V L
Sbjct: 64 LHHHHH-----TLASKSSTMVLEATMI-----CIDNSEWMRNGDYSPSRFQAQADAVNLI 213
Query: 118 IREFFDQNPLSHVG-LVTTKDGVANCLT---DLGGSPESHIKALMGKLECSGDASLQNAL 173
NP + VG L+ GV +T DLG I A M LE G+ +L +
Sbjct: 214 CGAKTQSNPENTVGVLIMAGKGVRVLVTPTSDLG-----KILACMHGLEIGGEMNLAAGI 378
Query: 174 ELVHSNLNQIPSYGHREVLILYSALSTCDPGDLMETI-QKCKKSKIRCSVIGLAAE 228
++ L + + +I+++ ++E I +K KK+ + V+ E
Sbjct: 379 QVAQLALKHRQNKKQHQRIIVFAGSHVKHEKKMLEMIGRKLKKNSVALDVVNFGEE 546
>TC93216 similar to PIR|T51606|T51606 probable 26S proteasome non-ATPase
chain S5a [imported] - rice, partial (45%)
Length = 661
Score = 35.8 bits (81), Expect = 0.036
Identities = 30/144 (20%), Positives = 62/144 (42%), Gaps = 4/144 (2%)
Frame = +3
Query: 89 IVVDLSKAASERDFRPSRMAVIAKQVELFIREFFDQNPLSHVGLVTTKDGVANCLTDLGG 148
I++D S+ D+ PSR + V L NP + VG++T L+
Sbjct: 108 IIMDNSEHMRNGDYSPSRFQAQSDTVSLICGAKTQSNPENTVGVMTMAGKGVRVLS---- 275
Query: 149 SPESHIKALMG---KLECSGDASLQNALELVHSNLNQIPSYGHREVLILYSALSTCDPGD 205
+P S + ++G LE G+ +L A+++ L + ++ ++++
Sbjct: 276 TPTSDLGKILGCMHGLEIGGEINLAAAIQVAQLALKHRQNKKQQQRIVVFCGSPVKHEKK 455
Query: 206 LMETI-QKCKKSKIRCSVIGLAAE 228
++E I +K KK+ + ++ E
Sbjct: 456 MLEMIGRKLKKNSVALDIVNFGEE 527
>TC88763 similar to GP|5091601|gb|AAD39590.1| This gene is a member of
PF|00501 AMP-binding enzymes. {Oryza sativa}, partial
(18%)
Length = 1019
Score = 33.5 bits (75), Expect = 0.18
Identities = 26/77 (33%), Positives = 34/77 (43%), Gaps = 1/77 (1%)
Frame = +1
Query: 14 EDDDEDEANDDGLEAWERAYTEDRSWESLQEDESGLL-RPIDTTAIHHAQYRRRLRALAS 72
ED D N++ LEA E E W S + SG L P T + HA+ R +R
Sbjct: 64 EDMDIMVVNEETLEAVEDG-VEGEIWVSSPSNASGYLGHPSLTREVFHARIRNTVRKCFL 240
Query: 73 NAATARIQKGLIRYLYI 89
I KG RYL++
Sbjct: 241 RTGDKGIVKGENRYLFV 291
>TC77535 similar to GP|17381208|gb|AAL36416.1 unknown protein {Arabidopsis
thaliana}, complete
Length = 1488
Score = 30.8 bits (68), Expect = 1.2
Identities = 25/84 (29%), Positives = 36/84 (42%), Gaps = 2/84 (2%)
Frame = +1
Query: 236 CQETGGTYSVALDESHFKELILEHSPPP--PAIAEYATANLIKMGFPQRAAEGSVAICTC 293
C++ G YSVAL ++H L +E+S P EY +L + G C C
Sbjct: 457 CKKCGSCYSVALRDNH---LCVENSMRHHCPICYEYLFDSLKDTTVMKC---GHTMHCEC 618
Query: 294 HEEAKTGGGYTCPRCKVRVCELPT 317
+ E Y CP C V ++ T
Sbjct: 619 YHEMIKRDKYCCPICSKSVIDMST 690
>TC77337 weakly similar to GP|4019275|gb|AAC95573.1| orf 48 {Ateline
herpesvirus 3}, partial (8%)
Length = 986
Score = 30.0 bits (66), Expect = 2.0
Identities = 19/44 (43%), Positives = 20/44 (45%), Gaps = 5/44 (11%)
Frame = +3
Query: 14 EDDDEDEANDDGLEAWER-----AYTEDRSWESLQEDESGLLRP 52
EDDDEDE +DD E E ED E EDE L P
Sbjct: 630 EDDDEDEDDDDEEEGGEEDEEEGVDEEDNEEEEEDEDEEALQPP 761
>TC80460 similar to GP|21537277|gb|AAM61618.1 unknown {Arabidopsis
thaliana}, partial (52%)
Length = 1867
Score = 30.0 bits (66), Expect = 2.0
Identities = 18/54 (33%), Positives = 24/54 (44%), Gaps = 3/54 (5%)
Frame = +1
Query: 289 AICTCHEEAKTGGGYTCPRCKVRVCELPTECRVCG---LTLISSPHLARSYHHL 339
A C CH++ GY C C CE +C C + I S HL+ +H L
Sbjct: 511 ASCFCHKQT-IDMGYVCSVCLSIFCEHHDKCSTCR*AYFSKIISFHLSLCFHKL 669
>TC87964 weakly similar to PIR|G96757|G96757 probable protein ATPase
T18K17.15 [imported] - Arabidopsis thaliana, partial
(24%)
Length = 1066
Score = 29.3 bits (64), Expect = 3.4
Identities = 14/44 (31%), Positives = 23/44 (51%), Gaps = 1/44 (2%)
Frame = -2
Query: 428 PYALDI-KLLSKWCSTLPPCYCDVASIYRTHEQVPVHEMELTNT 470
PY L + L++KW T PP + + +P+ E++L NT
Sbjct: 885 PYKLRVYTLMAKWHQTYPPATWTLNLKVESRSSIPITELKLFNT 754
>CB065276 similar to SP|Q37546|NU1M_ NADH-ubiquinone oxidoreductase chain 1
(EC 1.6.5.3). [Common earthworm] {Lumbricus terrestris},
partial (4%)
Length = 631
Score = 28.5 bits (62), Expect = 5.8
Identities = 11/28 (39%), Positives = 12/28 (42%)
Frame = +2
Query: 435 LLSKWCSTLPPCYCDVASIYRTHEQVPV 462
LL C PPC C YRT P+
Sbjct: 479 LLRSTCKGAPPCVCTSVRRYRTRSSAPI 562
>TC88481 similar to PIR|T00806|T02445 probable U4/U6 small nuclear
ribonucleoprotein [imported] - Arabidopsis thaliana,
partial (60%)
Length = 1836
Score = 28.5 bits (62), Expect = 5.8
Identities = 20/64 (31%), Positives = 28/64 (43%)
Frame = +1
Query: 8 PLNGDLEDDDEDEANDDGLEAWERAYTEDRSWESLQEDESGLLRPIDTTAIHHAQYRRRL 67
P NG++ D D +DDG + + E +ES L+R A+ RRR
Sbjct: 352 PQNGEVGSSDSDSDDDDGAD----TRINKGTGEYEISEESRLVRERHEKAMQDLMMRRRA 519
Query: 68 RALA 71
ALA
Sbjct: 520 AALA 531
>TC78348 weakly similar to GP|549986|gb|AAA80576.1|| possible
apospory-associated protein {Pennisetum ciliare},
partial (17%)
Length = 1368
Score = 28.5 bits (62), Expect = 5.8
Identities = 16/56 (28%), Positives = 28/56 (49%)
Frame = +1
Query: 5 AGKPLNGDLEDDDEDEANDDGLEAWERAYTEDRSWESLQEDESGLLRPIDTTAIHH 60
+G L D+E +DE ++ +E+ + Y + E+ EDES + T +HH
Sbjct: 601 SGLDLTEDMESEDEGYVTEEDMESEDEGYVTEEDMEN--EDESTVHHTEST--VHH 756
>TC77706 similar to GP|14532908|gb|AAK64136.1 unknown protein {Arabidopsis
thaliana}, partial (98%)
Length = 2358
Score = 28.5 bits (62), Expect = 5.8
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = -1
Query: 349 SPSSQNDPNHSFPNTCFGCQQSLL 372
SPS+Q+D N+ FPN+C ++ L
Sbjct: 837 SPSNQSDINNIFPNSCLKVMETAL 766
>TC89427 weakly similar to GP|4557063|gb|AAD22502.1| expressed protein
{Arabidopsis thaliana}, partial (50%)
Length = 753
Score = 28.1 bits (61), Expect = 7.6
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +1
Query: 3 NIAGKPLNGDLEDDDEDEANDDGLEAWERAYTED 36
N AG + D +DDD+D+ NDDG + E +D
Sbjct: 430 NGAGGSDDDDEDDDDDDDDNDDGEDEDEDEEEDD 531
>CA917961 homologue to GP|12325083|gb| hypothetical protein; 3218-172
{Arabidopsis thaliana}, partial (17%)
Length = 686
Score = 28.1 bits (61), Expect = 7.6
Identities = 15/41 (36%), Positives = 20/41 (48%), Gaps = 8/41 (19%)
Frame = +1
Query: 290 ICTC---HE-----EAKTGGGYTCPRCKVRVCELPTECRVC 322
IC+C HE +A G + C C+V V + CRVC
Sbjct: 400 ICSCASSHEHSSDLQASEDGMFYCSLCEVEVFKYSKHCRVC 522
>TC90331 weakly similar to GP|21751020|dbj|BAC03887. unnamed protein product
{Homo sapiens}, partial (25%)
Length = 557
Score = 28.1 bits (61), Expect = 7.6
Identities = 15/37 (40%), Positives = 18/37 (48%)
Frame = -1
Query: 8 PLNGDLEDDDEDEANDDGLEAWERAYTEDRSWESLQE 44
PLN D D+ E N+DG E E ED + QE
Sbjct: 440 PLNDGAADIDDGEENEDGEEEEEEVDDEDVDDDDAQE 330
>TC92413 weakly similar to GP|21618067|gb|AAM67117.1 unknown {Arabidopsis
thaliana}, partial (53%)
Length = 1070
Score = 28.1 bits (61), Expect = 7.6
Identities = 21/55 (38%), Positives = 30/55 (54%), Gaps = 9/55 (16%)
Frame = +1
Query: 1 MNNIAGKPL---NGDLEDDDEDEAND------DGLEAWERAYTEDRSWESLQEDE 46
+NN KP NG LEDD++++A D D LEA E T+ S + LQ+ +
Sbjct: 100 INNCIQKPTSSPNGLLEDDEDEDATDRLLNICDALEALE---TQLSSLQVLQQQQ 255
>BQ147693 similar to GP|5668770|gb T17H3.9 {Arabidopsis thaliana}, partial
(10%)
Length = 655
Score = 27.7 bits (60), Expect = 9.9
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = -1
Query: 435 LLSKWCSTLPPCYCDVASIYRTHEQV 460
L+S+WC+ + C C S R EQ+
Sbjct: 592 LVSEWCNDVGKCCCTCVSFARAEEQL 515
>TC91460 similar to GP|19698839|gb|AAL91155.1 cytochrome P450 {Arabidopsis
thaliana}, partial (59%)
Length = 976
Score = 27.7 bits (60), Expect = 9.9
Identities = 17/55 (30%), Positives = 23/55 (40%), Gaps = 5/55 (9%)
Frame = +1
Query: 316 PTECRVCGLTLISSPHLARSYH-----HLFPIVPFAEISPSSQNDPNHSFPNTCF 365
P C +C L+ SP ++ H P P + PSS+ D S N CF
Sbjct: 13 PNSCSLCLFPLVPSPRTNPNWSKSMALHR*PPGPIQKPEPSSRLDRGKSPSNRCF 177
>TC79422 similar to PIR|T47953|T47953 hypothetical protein F2A19.210 -
Arabidopsis thaliana, partial (60%)
Length = 1650
Score = 27.7 bits (60), Expect = 9.9
Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 10/64 (15%)
Frame = -1
Query: 276 KMGFPQRAAEGSV--AICTCHEEAKTGGGY---TCPRCKVRVCELP-----TECRVCGLT 325
++G P R G V A+ + + + TCP CK+ V E P T C + L+
Sbjct: 1239 QIGIPPRTGFGGV*TALVLVNNNSSPRSVFHERTCPPCKLTVAEAPRTFRRTTCSIPFLS 1060
Query: 326 LISS 329
LI+S
Sbjct: 1059 LITS 1048
>BF003643 weakly similar to PIR|F84686|F846 hypothetical protein At2g28580
[imported] - Arabidopsis thaliana, partial (11%)
Length = 545
Score = 27.7 bits (60), Expect = 9.9
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +2
Query: 430 ALDIKLLSKWCSTLPPCYCDVASIY 454
+L+I+L WC L CYC ++S Y
Sbjct: 362 SLEIQLNYCWCFYLLICYCPISSCY 436
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.135 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,096,054
Number of Sequences: 36976
Number of extensions: 284408
Number of successful extensions: 1734
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 1606
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1688
length of query: 471
length of database: 9,014,727
effective HSP length: 100
effective length of query: 371
effective length of database: 5,317,127
effective search space: 1972654117
effective search space used: 1972654117
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC135795.5