BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135565.11 + phase: 0
(225 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC81497 similar to GP|17104639|gb|AAL34208.1 unknown protein {Ar... 437 e-123
AJ497260 similar to GP|17104639|gb| unknown protein {Arabidopsis... 105 1e-23
BQ154320 similar to GP|3702337|gb| unknown protein {Arabidopsis ... 56 1e-08
TC79940 similar to GP|20466568|gb|AAM20601.1 unknown protein {Ar... 37 0.008
TC81209 similar to GP|7573630|dbj|BAA94539.1 EST AU082314(E2976)... 36 0.014
CB894760 similar to GP|18087579|gb At1g16320/F3O9_12 {Arabidopsi... 32 0.20
TC87757 similar to GP|15028227|gb|AAK76610.1 putative oligopepti... 32 0.26
BM814721 homologue to GP|14597044|em unnamed protein product {Ar... 27 4.8
BE204336 similar to PIR|T51392|T51 probable phytochelatin synthe... 27 6.3
TC76471 similar to PIR|T51392|T51392 probable phytochelatin synt... 27 6.3
BG453243 weakly similar to GP|6606570|gb|A unknown {Glycine max}... 27 8.2
>TC81497 similar to GP|17104639|gb|AAL34208.1 unknown protein {Arabidopsis
thaliana}, partial (65%)
Length = 1016
Score = 437 bits (1124), Expect = e-123
Identities = 217/218 (99%), Positives = 218/218 (99%)
Frame = +2
Query: 8 KTRTPPLQLRKQHCSNSYTPSSRNTCAIYNMVREKNETPQVLKIAVSGVTELLRLFSPPQ 67
+TRTPPLQLRKQHCSNSYTPSSRNTCAIYNMVREKNETPQVLKIAVSGVTELLRLFSPPQ
Sbjct: 122 QTRTPPLQLRKQHCSNSYTPSSRNTCAIYNMVREKNETPQVLKIAVSGVTELLRLFSPPQ 301
Query: 68 QTSVLSDDIEKQNNDSTVSSVEDVLIIIKSDYDNDYFVTGNFTSSIYTENCIFEDPTIKF 127
QTSVLSDDIEKQNNDSTVSSVEDVLIIIKSDYDNDYFVTGNFTSSIYTENCIFEDPTIKF
Sbjct: 302 QTSVLSDDIEKQNNDSTVSSVEDVLIIIKSDYDNDYFVTGNFTSSIYTENCIFEDPTIKF 481
Query: 128 SGRDLYARNLKLLVPFFDCASIKLLKIEKEVESDTNFLRASWKLRTNLKLPWRPLIAIDG 187
SGRDLYARNLKLLVPFFDCASIKLLKIEKEVESDTNFLRASWKLRTNLKLPWRPLIAIDG
Sbjct: 482 SGRDLYARNLKLLVPFFDCASIKLLKIEKEVESDTNFLRASWKLRTNLKLPWRPLIAIDG 661
Query: 188 STSYELNEDFKIVRHVESWNVSALEAVLQIFKFEKPGG 225
STSYELNEDFKIVRHVESWNVSALEAVLQIFKFEKPGG
Sbjct: 662 STSYELNEDFKIVRHVESWNVSALEAVLQIFKFEKPGG 775
>AJ497260 similar to GP|17104639|gb| unknown protein {Arabidopsis thaliana},
partial (21%)
Length = 606
Score = 105 bits (262), Expect = 1e-23
Identities = 65/124 (52%), Positives = 65/124 (52%), Gaps = 59/124 (47%)
Frame = +3
Query: 161 DTNFLRASWKLR---------------------------TNLKLPWRPLIAIDGSTSYEL 193
DTNFLRASWKLR TNLKLPWRPLIAIDGSTSYEL
Sbjct: 18 DTNFLRASWKLRCLPSSLSMLICNIWIYFFKHFIIPQCRTNLKLPWRPLIAIDGSTSYEL 197
Query: 194 NEDFK--------------------------------IVRHVESWNVSALEAVLQIFKFE 221
NEDFK IVRHVESWNVSALEAVLQIFKFE
Sbjct: 198 NEDFKVRIKMPLCLKTFHS*CDLKSMSTSG*NIFV*QIVRHVESWNVSALEAVLQIFKFE 377
Query: 222 KPGG 225
KPGG
Sbjct: 378 KPGG 389
>BQ154320 similar to GP|3702337|gb| unknown protein {Arabidopsis thaliana},
partial (37%)
Length = 628
Score = 56.2 bits (134), Expect = 1e-08
Identities = 31/112 (27%), Positives = 51/112 (44%), Gaps = 2/112 (1%)
Frame = +2
Query: 73 SDDIEKQNNDSTVSSVEDVLII--IKSDYDNDYFVTGNFTSSIYTENCIFEDPTIKFSGR 130
SD+ E + +++ ++ IK D+ YFVTG+ T + Y E+C F DP F G
Sbjct: 251 SDEEEASPPQRLLQTIDRASVVDTIKQDFQRSYFVTGDLTLNAYEEDCEFADPAGSFKGL 430
Query: 131 DLYARNLKLLVPFFDCASIKLLKIEKEVESDTNFLRASWKLRTNLKLPWRPL 182
+ RN + +++ L+K E + W+ L PWRP+
Sbjct: 431 QRFKRNCTNFGSLLEKSTMNLMKWEDFEDKGI----GHWRFSCILSFPWRPI 574
>TC79940 similar to GP|20466568|gb|AAM20601.1 unknown protein {Arabidopsis
thaliana}, partial (64%)
Length = 1149
Score = 36.6 bits (83), Expect = 0.008
Identities = 28/120 (23%), Positives = 54/120 (44%), Gaps = 7/120 (5%)
Frame = +1
Query: 106 TGNFTSSIYTENCIFEDPTIKFSGRDLYARNLKLLVPFFDCASIKLLKIEKEVESDTNFL 165
+GN Y + ++DP FSGR+ Y R L + + + + +++ T+ L
Sbjct: 340 SGNRALGTYAVSVTYKDPIRSFSGREKYKRPLWAITALENPS----VTVQEMTMLSTSVL 507
Query: 166 RASWKLRTNLKLPWRPLIAIDG------STSYELNE-DFKIVRHVESWNVSALEAVLQIF 218
W +R P L + G ++ + LN+ ++++H E W++S+ A Q F
Sbjct: 508 SIKWTIRGK---PKSILAGVGGGLILRVTSKFTLNQISGQVIKHEELWDLSSSPASAQAF 678
>TC81209 similar to GP|7573630|dbj|BAA94539.1 EST AU082314(E2976)
corresponds to a region of the predicted gene.~Similar
to Arabidopsis thaliana, partial (51%)
Length = 723
Score = 35.8 bits (81), Expect = 0.014
Identities = 25/94 (26%), Positives = 43/94 (45%), Gaps = 4/94 (4%)
Frame = +2
Query: 113 IYTENCIFEDPTIKFSGRDLYAR---NLKLLVP-FFDCASIKLLKIEKEVESDTNFLRAS 168
IY ++ + +DP F G + Y NL+ FF I + + + VE N +
Sbjct: 428 IYRDDIVLKDPLNTFMGIENYKSIFWNLRFHGKIFFKALWIDINSVWQPVE---NVIMVR 598
Query: 169 WKLRTNLKLPWRPLIAIDGSTSYELNEDFKIVRH 202
W + ++PW DG++ Y+L++ KI H
Sbjct: 599 WTVHGIPRVPWESRGRFDGTSEYKLDKQGKIYEH 700
>CB894760 similar to GP|18087579|gb At1g16320/F3O9_12 {Arabidopsis thaliana},
partial (71%)
Length = 807
Score = 32.0 bits (71), Expect = 0.20
Identities = 28/119 (23%), Positives = 48/119 (39%), Gaps = 5/119 (4%)
Frame = +3
Query: 89 EDVLIIIKSDYDNDYFVTGNFTSSIYTENCIFEDPTIKFSGRDLY-----ARNLKLLVPF 143
ED+ +I D + D IY ++ F DP F+G + Y A + F
Sbjct: 276 EDMPLIFVKDLNYD----------IYRDDVTFLDPLNTFTGIEKYKLIFWALRFHGRILF 425
Query: 144 FDCASIKLLKIEKEVESDTNFLRASWKLRTNLKLPWRPLIAIDGSTSYELNEDFKIVRH 202
+ L + + + N + W LR ++PW G++ Y+L+ + KI H
Sbjct: 426 REIG----LDVYRIWQPSENVILIRWNLRGVPRVPWEAKGEFQGTSRYKLDRNGKIYEH 590
>TC87757 similar to GP|15028227|gb|AAK76610.1 putative oligopeptidase A
{Arabidopsis thaliana}, partial (52%)
Length = 1391
Score = 31.6 bits (70), Expect = 0.26
Identities = 25/87 (28%), Positives = 41/87 (46%), Gaps = 1/87 (1%)
Frame = -2
Query: 121 EDPTIKFSGRDLYARNLKLLVPFFDCASIKLLKIEKE-VESDTNFLRASWKLRTNLKLPW 179
+ P+I F GR+ R+ L+ +FD S+ L K+ + V+S + A W L L+
Sbjct: 283 KSPSITF-GREKNGRSSSSLMSYFDSRSLSLQKLTSQCVKSSAS--NAPWSLENFLRSSI 113
Query: 180 RPLIAIDGSTSYELNEDFKIVRHVESW 206
I GS + L + F + + SW
Sbjct: 112 SCTAGIPGSFTKLL*KFFCFINRLPSW 32
>BM814721 homologue to GP|14597044|em unnamed protein product {Arabidopsis
thaliana}, partial (17%)
Length = 771
Score = 27.3 bits (59), Expect = 4.8
Identities = 19/89 (21%), Positives = 43/89 (47%), Gaps = 7/89 (7%)
Frame = +1
Query: 41 EKNETPQVLKIAVSGVTELLRLFSPPQQTSVLS---DDIEKQNNDSTVSSVEDVLIIIKS 97
+ N+T ++L+ + + + SPP Q ++ + + I +QN + + +I S
Sbjct: 427 DNNQTSEILRFGNARTAQFNNILSPPPQQTLQTPPFNFITQQNQNYHHDQSQFQGLISLS 606
Query: 98 DYDNDYFVTGNF----TSSIYTENCIFED 122
+ +N+ + NF ++ ++EN I D
Sbjct: 607 ELNNNNMFSENFNNEGSNDFFSENSIMFD 693
>BE204336 similar to PIR|T51392|T51 probable phytochelatin synthetase -
Arabidopsis thaliana, partial (38%)
Length = 684
Score = 26.9 bits (58), Expect = 6.3
Identities = 18/61 (29%), Positives = 28/61 (45%), Gaps = 4/61 (6%)
Frame = +1
Query: 165 LRASWKLRTNLKLPWRPLIAIDGSTSYELNEDFKIVRHVESWNVSA----LEAVLQIFKF 220
+R W ++TN K WR IA+ + +Y LN W ++ L V Q+F F
Sbjct: 490 IRVHWHVKTNYKDYWRVKIAVT-NFNYRLNYSL--------WTLAVQHPNLNNVTQVFSF 642
Query: 221 E 221
+
Sbjct: 643 D 645
>TC76471 similar to PIR|T51392|T51392 probable phytochelatin synthetase -
Arabidopsis thaliana, partial (94%)
Length = 1772
Score = 26.9 bits (58), Expect = 6.3
Identities = 18/61 (29%), Positives = 28/61 (45%), Gaps = 4/61 (6%)
Frame = +1
Query: 165 LRASWKLRTNLKLPWRPLIAIDGSTSYELNEDFKIVRHVESWNVSA----LEAVLQIFKF 220
+R W ++TN K WR IA+ + +Y LN W ++ L V Q+F F
Sbjct: 973 IRVHWHVKTNYKDYWRVKIAVT-NFNYRLNYSL--------WTLAVQHPNLNNVTQVFSF 1125
Query: 221 E 221
+
Sbjct: 1126 D 1128
>BG453243 weakly similar to GP|6606570|gb|A unknown {Glycine max}, partial
(8%)
Length = 661
Score = 26.6 bits (57), Expect = 8.2
Identities = 22/64 (34%), Positives = 29/64 (44%), Gaps = 7/64 (10%)
Frame = +1
Query: 123 PTIKFSGRDLYARNLKLLVPFFDCASIKLLKIE----KEVESDTNFLRASWKLRTNL--- 175
P K DL NLKL+ + S+ LKIE K+V D + AS L +L
Sbjct: 70 PPFKLQLLDLKCANLKLIPWLYTQTSLTTLKIENSTFKDVSQDKFWSLASHCLFLSLFHN 249
Query: 176 KLPW 179
+PW
Sbjct: 250 NMPW 261
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.134 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,878,712
Number of Sequences: 36976
Number of extensions: 88110
Number of successful extensions: 486
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 476
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 483
length of query: 225
length of database: 9,014,727
effective HSP length: 93
effective length of query: 132
effective length of database: 5,575,959
effective search space: 736026588
effective search space used: 736026588
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC135565.11