BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135505.7 - phase: 0 /pseudo
(109 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80997 similar to PIR|B84729|B84729 70kD heat shock protein [im... 106 2e-24
TC80052 similar to PIR|JC4786|JC4786 dnaK-type molecular chapero... 38 5e-04
TC80733 homologue to SP|Q01233|HS70_NEUCR Heat shock 70 kDa prot... 32 0.038
TC83178 homologue to SP|P03731|VMTZ_LAMBD Minor tail protein Z (... 29 0.32
TC84397 similar to PIR|A96704|A96704 hypothetical protein T23K23... 26 2.1
TC88597 weakly similar to PIR|B96700|B96700 protein F12A21.17 [i... 25 3.5
CB894488 similar to GP|6437560|gb| hypothetical protein {Arabido... 25 4.6
TC87404 similar to GP|22137130|gb|AAM91410.1 At1g47330/T3F24_2 {... 24 7.8
CA859789 24 7.8
>TC80997 similar to PIR|B84729|B84729 70kD heat shock protein [imported] -
Arabidopsis thaliana, partial (91%)
Length = 1717
Score = 106 bits (264), Expect = 2e-24
Identities = 56/104 (53%), Positives = 71/104 (67%)
Frame = +3
Query: 1 MHTLDIKVRPFIAAIANKACRSTTAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFT 60
+ TLDI VRPFIAA+ N RSTT E+L FLV+L+ E +K PIR VV TVPVSF+
Sbjct: 252 VQTLDIGVRPFIAALVNNVWRSTTPEEVLAMFLVELRLMTETHLKRPIRNVVLTVPVSFS 431
Query: 61 RLRRTQIERASAWADLDDVELMPQPIAVALFYAQQQLQTSASSL 104
R + T++ERA A A L + LMP+P AVAL Y QQQ + S ++
Sbjct: 432 RFQLTRLERACAMAGLHVLRLMPEPTAVALLYGQQQQKASQETM 563
>TC80052 similar to PIR|JC4786|JC4786 dnaK-type molecular chaperone hsc70-3
- tomato, partial (27%)
Length = 667
Score = 38.1 bits (87), Expect = 5e-04
Identities = 25/88 (28%), Positives = 40/88 (45%)
Frame = +3
Query: 5 DIKVRPFIAAIANKACRSTTAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRR 64
D+ +P I N + A E+ LVK++ E + + VV TVP F +R
Sbjct: 378 DLNDKPMIVVNYNDEEKHFAAEEISSMVLVKMREIAETFLGSIVEDVVITVPAYFNDSQR 557
Query: 65 TQIERASAWADLDDVELMPQPIAVALFY 92
A A A L+ + ++ +P A A+ Y
Sbjct: 558 QSTRDAGAIAGLNVMRIINEPTAAAIAY 641
>TC80733 homologue to SP|Q01233|HS70_NEUCR Heat shock 70 kDa protein
(HSP70). {Neurospora crassa}, partial (54%)
Length = 1258
Score = 32.0 bits (71), Expect = 0.038
Identities = 19/72 (26%), Positives = 35/72 (48%)
Frame = +2
Query: 21 RSTTAVELLGKFLVKLKFSVEIRVKIPIRKVVFTVPVSFTRLRRTQIERASAWADLDDVE 80
++ T E+ LVK++ + E + + V TVP F +R + A A L+ +
Sbjct: 506 KTFTPEEISAMVLVKMRETAEAYLGGQVTNAVITVPAYFNDSQRQATKDAGLIAGLNVLR 685
Query: 81 LMPQPIAVALFY 92
++ +P A A+ Y
Sbjct: 686 IINEPTAAAIAY 721
>TC83178 homologue to SP|P03731|VMTZ_LAMBD Minor tail protein Z (GPZ).
{Bacteriophage lambda}, partial (92%)
Length = 1031
Score = 28.9 bits (63), Expect = 0.32
Identities = 14/38 (36%), Positives = 24/38 (62%)
Frame = -1
Query: 52 VFTVPVSFTRLRRTQIERASAWADLDDVELMPQPIAVA 89
+F++ +SFT RRT + RA+ AD D + + +A+A
Sbjct: 143 LFSLALSFTSFRRTFVSRATCDADCDIADEATRLMAIA 30
>TC84397 similar to PIR|A96704|A96704 hypothetical protein T23K23.7
[imported] - Arabidopsis thaliana, partial (12%)
Length = 664
Score = 26.2 bits (56), Expect = 2.1
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = -1
Query: 7 KVRPFIAAIANKACRSTTAVELLGKFLVKLKFSVE 41
+VRP + + + AC ST A + +F KLKF+ E
Sbjct: 346 EVRPIVITMPSNACSSTPA-D*TSEFNKKLKFTFE 245
>TC88597 weakly similar to PIR|B96700|B96700 protein F12A21.17 [imported] -
Arabidopsis thaliana, partial (56%)
Length = 1459
Score = 25.4 bits (54), Expect = 3.5
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +3
Query: 73 WADLDDVELMPQPIAVALFYAQQQLQTSASSLE 105
W D +D+E PIAV L Y QQ L ++E
Sbjct: 756 WPD-EDIENELSPIAVQLGYVQQLLGRKQDAIE 851
>CB894488 similar to GP|6437560|gb| hypothetical protein {Arabidopsis
thaliana}, partial (37%)
Length = 830
Score = 25.0 bits (53), Expect = 4.6
Identities = 10/40 (25%), Positives = 20/40 (50%)
Frame = -2
Query: 2 HTLDIKVRPFIAAIANKACRSTTAVELLGKFLVKLKFSVE 41
H +++ +RP + K + ++ +GK LV +K E
Sbjct: 784 HVVEVALRPVPQFVVKKVQMGSVRLDTMGKVLVHVKMKPE 665
>TC87404 similar to GP|22137130|gb|AAM91410.1 At1g47330/T3F24_2 {Arabidopsis
thaliana}, partial (70%)
Length = 1944
Score = 24.3 bits (51), Expect = 7.8
Identities = 9/25 (36%), Positives = 17/25 (68%)
Frame = +3
Query: 28 LLGKFLVKLKFSVEIRVKIPIRKVV 52
++G LVK F V+ + +P+RK++
Sbjct: 849 IMGLVLVKNLFMVDSKAAVPLRKMI 923
>CA859789
Length = 259
Score = 24.3 bits (51), Expect = 7.8
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +3
Query: 19 ACRSTTAVELLGKFLVKLKFSVEIRVKIPIRKVVFT 54
A R+ + LL KFL+K + IRV+ + + F+
Sbjct: 96 AIRTKNKIVLLAKFLLKFIL*ITIRVRQKFQPIKFS 203
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.134 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,457,939
Number of Sequences: 36976
Number of extensions: 23175
Number of successful extensions: 161
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of query: 109
length of database: 9,014,727
effective HSP length: 85
effective length of query: 24
effective length of database: 5,871,767
effective search space: 140922408
effective search space used: 140922408
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 50 (23.9 bits)
Medicago: description of AC135505.7