
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135504.12 - phase: 0
(179 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG587101 similar to GP|6691191|gb F7F22.15 {Arabidopsis thaliana... 109 7e-25
BG644720 46 6e-07
TC77595 weakly similar to PIR|T18350|T18350 probable pol polypro... 29 0.87
TC79034 similar to GP|10177293|dbj|BAB10554. ATP-dependent RNA h... 28 1.9
TC89037 similar to GP|13605811|gb|AAK32891.1 AT5g36940/MLF18_60 ... 27 4.3
TC83305 similar to GP|7230559|gb|AAF43007.1| serum opacity facto... 27 5.7
BG587364 similar to GP|22136924|gb putative RNA helicase {Arabid... 26 7.4
BQ144172 weakly similar to PIR|D86416|D864 probable beta-1 3 glu... 26 7.4
TC86605 similar to PIR|T01853|T01853 probable hexose transport p... 26 9.7
TC77543 homologue to GP|22901738|gb|AAN10048.1 high molecular we... 26 9.7
>BG587101 similar to GP|6691191|gb F7F22.15 {Arabidopsis thaliana}, partial
(10%)
Length = 624
Score = 109 bits (272), Expect = 7e-25
Identities = 57/159 (35%), Positives = 84/159 (51%)
Frame = +2
Query: 2 EGTLYKRGFITPLIKCLGPNETQEVLADVHDGICGQHLDVKSLARKVLRAGYYWPTMLKD 61
E LYK+ I+C+ E +L H H V K+ +AG++WPTM KD
Sbjct: 32 EPFLYKQCADNIYIRCVAEEEIPGILFHCHGSNYAGHFAVSKTVSKIQQAGFWWPTMFKD 211
Query: 62 AEDYVNLCDKCQRHGDMHLAPPVELTSLVSPWPFAWWGIDLLGPFPKATGQLKYLVVAIN 121
A +++ CD CQR G++ + ++ F WGID +GPFP + KY++VA++
Sbjct: 212 AHSFISKCDPCQRQGNIS*RNEMPQNFILEVEVFDVWGIDFMGPFPSSYNN-KYILVAVD 388
Query: 122 YSTKWIEVEPLAKIKAKNVLRFFKRNILARFGVPALVIS 160
Y +KW+E A V++ FK I RFGVP +VIS
Sbjct: 389 YVSKWVEAIASPTNDATVVVKMFKSVIFPRFGVPRVVIS 505
>BG644720
Length = 678
Score = 45.8 bits (107), Expect(2) = 6e-07
Identities = 37/110 (33%), Positives = 50/110 (44%), Gaps = 2/110 (1%)
Frame = -3
Query: 4 TLYKRGFITPLIKCLGPNETQEVLADVHDGICGQHLDVKSLARKVLRAGYYWPT--MLKD 61
TLY F L+ CLG ET + H +CG H L + R GYYW T MLK
Sbjct: 355 TLYI*LFEGVLL*CLGEEETIQAFQ*AHSRVCGSH--KSKLYFHIKRMGYYWNT*IMLKG 182
Query: 62 AEDYVNLCDKCQRHGDMHLAPPVELTSLVSPWPFAWWGIDLLGPFPKATG 111
A L + Q+ PP L ++ F W +++ G FPK++G
Sbjct: 181 A----LLANFIQQ-------PPEVLHPTITS*LFESWELNVFGLFPKSSG 65
Score = 23.5 bits (49), Expect(2) = 6e-07
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = -1
Query: 106 FPKATGQLKYLVVAINYSTKWIEVEPL 132
FP Y++ A+ Y KW+EV L
Sbjct: 81 FPNLLVDTWYILAAVYYF*KWVEVVAL 1
>TC77595 weakly similar to PIR|T18350|T18350 probable pol polyprotein - rice
blast fungus gypsy retroelement (fragment), partial
(14%)
Length = 1708
Score = 29.3 bits (64), Expect = 0.87
Identities = 31/153 (20%), Positives = 62/153 (40%), Gaps = 11/153 (7%)
Frame = +2
Query: 25 EVLADVHDGICGQHLDVKSLARKVLRAGYYWPTMLKDAEDYVNLCDKCQRHGDMHLAPPV 84
+++ + HD H V R ++WP + +V CD C G +H+
Sbjct: 176 KLVQESHDSTAAGHPGRNGTLEIVSRK-FFWPGQSQTVRRFVRNCDVC---GGIHIWRQA 343
Query: 85 ELTSLVSPWP-----FAWWGIDLLGPFPKATGQ-LKYLVVAINYSTKWIEVEPLAKIKAK 138
+ + P P + +D + P G+ +YL V ++ +K + +E + ++A+
Sbjct: 344 K-RGFLKPLPVPNRLHSDLSMDFITSLPPTRGRGSQYLWVIVDRLSKSVTLEEMDTMEAE 520
Query: 139 NVLRFFKRNILARFGVPALVIS-----VLSRFW 166
+ F G+P ++S + RFW
Sbjct: 521 ACAQRFLSCHYRFHGMPQSIVSDRGSNWVGRFW 619
>TC79034 similar to GP|10177293|dbj|BAB10554. ATP-dependent RNA
helicase-like protein {Arabidopsis thaliana}, partial
(65%)
Length = 1443
Score = 28.1 bits (61), Expect = 1.9
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = +2
Query: 52 GYYWPTMLKDAEDYVNLCDKCQRHGDM 78
G YW T+ + Y + C C RHG++
Sbjct: 527 GSYWVTISESESIYKSSC*SCHRHGEI 607
>TC89037 similar to GP|13605811|gb|AAK32891.1 AT5g36940/MLF18_60
{Arabidopsis thaliana}, partial (39%)
Length = 1337
Score = 26.9 bits (58), Expect = 4.3
Identities = 9/25 (36%), Positives = 14/25 (56%)
Frame = +1
Query: 80 LAPPVELTSLVSPWPFAWWGIDLLG 104
L P+ L + PW F+W ++ LG
Sbjct: 286 LRVPLSLALFLQPWHFSWMSLNFLG 360
>TC83305 similar to GP|7230559|gb|AAF43007.1| serum opacity factor
{Streptococcus pyogenes}, partial (10%)
Length = 741
Score = 26.6 bits (57), Expect = 5.7
Identities = 8/24 (33%), Positives = 13/24 (53%)
Frame = +1
Query: 75 HGDMHLAPPVELTSLVSPWPFAWW 98
H H + T+ +SPW ++WW
Sbjct: 409 HQSSHYQNLLPATN*ISPWQYSWW 480
>BG587364 similar to GP|22136924|gb putative RNA helicase {Arabidopsis
thaliana}, partial (1%)
Length = 626
Score = 26.2 bits (56), Expect = 7.4
Identities = 9/21 (42%), Positives = 14/21 (65%)
Frame = -2
Query: 6 YKRGFITPLIKCLGPNETQEV 26
YK G +PL+ C+ PNE + +
Sbjct: 349 YKGGVTSPLLVCIPPNEEKSI 287
>BQ144172 weakly similar to PIR|D86416|D864 probable beta-1 3 glucanase
26636-27432 [imported] - Arabidopsis thaliana, partial
(15%)
Length = 1333
Score = 26.2 bits (56), Expect = 7.4
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -3
Query: 99 GIDLLGPFPKATGQLKYL 116
G DLLGPFP GQ++ L
Sbjct: 539 GGDLLGPFPGLPGQIRQL 486
>TC86605 similar to PIR|T01853|T01853 probable hexose transport protein
F9D12.17 - Arabidopsis thaliana, partial (91%)
Length = 3111
Score = 25.8 bits (55), Expect = 9.7
Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 7/57 (12%)
Frame = +3
Query: 127 IEVEPLAKIKAKNVLRFFK---RNILARFGVPALVIS----VLSRFWGIKSLIRLEP 176
IE E L ++A V + K RN+L R P LVIS + +F GI +++ P
Sbjct: 2040 IEPEFLELVEASRVAKEVKHPFRNLLKRNNRPQLVISIALMIFQQFTGINAIMFYAP 2210
>TC77543 homologue to GP|22901738|gb|AAN10048.1 high molecular weight root
vegetative storage protein precursor {Medicago sativa},
partial (70%)
Length = 737
Score = 25.8 bits (55), Expect = 9.7
Identities = 12/31 (38%), Positives = 17/31 (54%), Gaps = 2/31 (6%)
Frame = +2
Query: 80 LAPPVELTSLVSPWP--FAWWGIDLLGPFPK 108
+AP V L ++ + F WW LLGP P+
Sbjct: 41 IAPSVSLNNISLHYQSIFKWWHCHLLGPKPR 133
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.143 0.463
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,144,721
Number of Sequences: 36976
Number of extensions: 85759
Number of successful extensions: 508
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 505
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 505
length of query: 179
length of database: 9,014,727
effective HSP length: 90
effective length of query: 89
effective length of database: 5,686,887
effective search space: 506132943
effective search space used: 506132943
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 55 (25.8 bits)
Medicago: description of AC135504.12