Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC135504.12 - phase: 0 
         (179 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BG587101 similar to GP|6691191|gb F7F22.15 {Arabidopsis thaliana...   109  7e-25
BG644720                                                               46  6e-07
TC77595 weakly similar to PIR|T18350|T18350 probable pol polypro...    29  0.87
TC79034 similar to GP|10177293|dbj|BAB10554. ATP-dependent RNA h...    28  1.9
TC89037 similar to GP|13605811|gb|AAK32891.1 AT5g36940/MLF18_60 ...    27  4.3
TC83305 similar to GP|7230559|gb|AAF43007.1| serum opacity facto...    27  5.7
BG587364 similar to GP|22136924|gb putative RNA helicase {Arabid...    26  7.4
BQ144172 weakly similar to PIR|D86416|D864 probable beta-1 3 glu...    26  7.4
TC86605 similar to PIR|T01853|T01853 probable hexose transport p...    26  9.7
TC77543 homologue to GP|22901738|gb|AAN10048.1 high molecular we...    26  9.7

>BG587101 similar to GP|6691191|gb F7F22.15 {Arabidopsis thaliana}, partial
           (10%)
          Length = 624

 Score =  109 bits (272), Expect = 7e-25
 Identities = 57/159 (35%), Positives = 84/159 (51%)
 Frame = +2

Query: 2   EGTLYKRGFITPLIKCLGPNETQEVLADVHDGICGQHLDVKSLARKVLRAGYYWPTMLKD 61
           E  LYK+      I+C+   E   +L   H      H  V     K+ +AG++WPTM KD
Sbjct: 32  EPFLYKQCADNIYIRCVAEEEIPGILFHCHGSNYAGHFAVSKTVSKIQQAGFWWPTMFKD 211

Query: 62  AEDYVNLCDKCQRHGDMHLAPPVELTSLVSPWPFAWWGIDLLGPFPKATGQLKYLVVAIN 121
           A  +++ CD CQR G++     +    ++    F  WGID +GPFP +    KY++VA++
Sbjct: 212 AHSFISKCDPCQRQGNIS*RNEMPQNFILEVEVFDVWGIDFMGPFPSSYNN-KYILVAVD 388

Query: 122 YSTKWIEVEPLAKIKAKNVLRFFKRNILARFGVPALVIS 160
           Y +KW+E        A  V++ FK  I  RFGVP +VIS
Sbjct: 389 YVSKWVEAIASPTNDATVVVKMFKSVIFPRFGVPRVVIS 505


>BG644720 
          Length = 678

 Score = 45.8 bits (107), Expect(2) = 6e-07
 Identities = 37/110 (33%), Positives = 50/110 (44%), Gaps = 2/110 (1%)
 Frame = -3

Query: 4   TLYKRGFITPLIKCLGPNETQEVLADVHDGICGQHLDVKSLARKVLRAGYYWPT--MLKD 61
           TLY   F   L+ CLG  ET +     H  +CG H     L   + R GYYW T  MLK 
Sbjct: 355 TLYI*LFEGVLL*CLGEEETIQAFQ*AHSRVCGSH--KSKLYFHIKRMGYYWNT*IMLKG 182

Query: 62  AEDYVNLCDKCQRHGDMHLAPPVELTSLVSPWPFAWWGIDLLGPFPKATG 111
           A     L +  Q+       PP  L   ++   F  W +++ G FPK++G
Sbjct: 181 A----LLANFIQQ-------PPEVLHPTITS*LFESWELNVFGLFPKSSG 65



 Score = 23.5 bits (49), Expect(2) = 6e-07
 Identities = 10/27 (37%), Positives = 14/27 (51%)
 Frame = -1

Query: 106 FPKATGQLKYLVVAINYSTKWIEVEPL 132
           FP       Y++ A+ Y  KW+EV  L
Sbjct: 81  FPNLLVDTWYILAAVYYF*KWVEVVAL 1


>TC77595 weakly similar to PIR|T18350|T18350 probable pol polyprotein - rice
           blast fungus gypsy retroelement (fragment), partial
           (14%)
          Length = 1708

 Score = 29.3 bits (64), Expect = 0.87
 Identities = 31/153 (20%), Positives = 62/153 (40%), Gaps = 11/153 (7%)
 Frame = +2

Query: 25  EVLADVHDGICGQHLDVKSLARKVLRAGYYWPTMLKDAEDYVNLCDKCQRHGDMHLAPPV 84
           +++ + HD     H         V R  ++WP   +    +V  CD C   G +H+    
Sbjct: 176 KLVQESHDSTAAGHPGRNGTLEIVSRK-FFWPGQSQTVRRFVRNCDVC---GGIHIWRQA 343

Query: 85  ELTSLVSPWP-----FAWWGIDLLGPFPKATGQ-LKYLVVAINYSTKWIEVEPLAKIKAK 138
           +    + P P      +   +D +   P   G+  +YL V ++  +K + +E +  ++A+
Sbjct: 344 K-RGFLKPLPVPNRLHSDLSMDFITSLPPTRGRGSQYLWVIVDRLSKSVTLEEMDTMEAE 520

Query: 139 NVLRFFKRNILARFGVPALVIS-----VLSRFW 166
              + F        G+P  ++S      + RFW
Sbjct: 521 ACAQRFLSCHYRFHGMPQSIVSDRGSNWVGRFW 619


>TC79034 similar to GP|10177293|dbj|BAB10554. ATP-dependent RNA
           helicase-like protein {Arabidopsis thaliana}, partial
           (65%)
          Length = 1443

 Score = 28.1 bits (61), Expect = 1.9
 Identities = 10/27 (37%), Positives = 15/27 (55%)
 Frame = +2

Query: 52  GYYWPTMLKDAEDYVNLCDKCQRHGDM 78
           G YW T+ +    Y + C  C RHG++
Sbjct: 527 GSYWVTISESESIYKSSC*SCHRHGEI 607


>TC89037 similar to GP|13605811|gb|AAK32891.1 AT5g36940/MLF18_60
           {Arabidopsis thaliana}, partial (39%)
          Length = 1337

 Score = 26.9 bits (58), Expect = 4.3
 Identities = 9/25 (36%), Positives = 14/25 (56%)
 Frame = +1

Query: 80  LAPPVELTSLVSPWPFAWWGIDLLG 104
           L  P+ L   + PW F+W  ++ LG
Sbjct: 286 LRVPLSLALFLQPWHFSWMSLNFLG 360


>TC83305 similar to GP|7230559|gb|AAF43007.1| serum opacity factor
           {Streptococcus pyogenes}, partial (10%)
          Length = 741

 Score = 26.6 bits (57), Expect = 5.7
 Identities = 8/24 (33%), Positives = 13/24 (53%)
 Frame = +1

Query: 75  HGDMHLAPPVELTSLVSPWPFAWW 98
           H   H    +  T+ +SPW ++WW
Sbjct: 409 HQSSHYQNLLPATN*ISPWQYSWW 480


>BG587364 similar to GP|22136924|gb putative RNA helicase {Arabidopsis
           thaliana}, partial (1%)
          Length = 626

 Score = 26.2 bits (56), Expect = 7.4
 Identities = 9/21 (42%), Positives = 14/21 (65%)
 Frame = -2

Query: 6   YKRGFITPLIKCLGPNETQEV 26
           YK G  +PL+ C+ PNE + +
Sbjct: 349 YKGGVTSPLLVCIPPNEEKSI 287


>BQ144172 weakly similar to PIR|D86416|D864 probable beta-1 3 glucanase
           26636-27432 [imported] - Arabidopsis thaliana, partial
           (15%)
          Length = 1333

 Score = 26.2 bits (56), Expect = 7.4
 Identities = 11/18 (61%), Positives = 13/18 (72%)
 Frame = -3

Query: 99  GIDLLGPFPKATGQLKYL 116
           G DLLGPFP   GQ++ L
Sbjct: 539 GGDLLGPFPGLPGQIRQL 486


>TC86605 similar to PIR|T01853|T01853 probable hexose transport protein
            F9D12.17 - Arabidopsis thaliana, partial (91%)
          Length = 3111

 Score = 25.8 bits (55), Expect = 9.7
 Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 7/57 (12%)
 Frame = +3

Query: 127  IEVEPLAKIKAKNVLRFFK---RNILARFGVPALVIS----VLSRFWGIKSLIRLEP 176
            IE E L  ++A  V +  K   RN+L R   P LVIS    +  +F GI +++   P
Sbjct: 2040 IEPEFLELVEASRVAKEVKHPFRNLLKRNNRPQLVISIALMIFQQFTGINAIMFYAP 2210


>TC77543 homologue to GP|22901738|gb|AAN10048.1 high molecular weight root
           vegetative storage protein precursor {Medicago sativa},
           partial (70%)
          Length = 737

 Score = 25.8 bits (55), Expect = 9.7
 Identities = 12/31 (38%), Positives = 17/31 (54%), Gaps = 2/31 (6%)
 Frame = +2

Query: 80  LAPPVELTSLVSPWP--FAWWGIDLLGPFPK 108
           +AP V L ++   +   F WW   LLGP P+
Sbjct: 41  IAPSVSLNNISLHYQSIFKWWHCHLLGPKPR 133


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.325    0.143    0.463 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,144,721
Number of Sequences: 36976
Number of extensions: 85759
Number of successful extensions: 508
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 505
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 505
length of query: 179
length of database: 9,014,727
effective HSP length: 90
effective length of query: 89
effective length of database: 5,686,887
effective search space: 506132943
effective search space used: 506132943
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 55 (25.8 bits)


Medicago: description of AC135504.12