TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC135465.2 + phase: 0 
         (194 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC88736 homologue to GP|13277515|gb|AAK16221.1 PII protein {Medi...   380  e-106
BQ144829                                                               60  5e-10
TC85168 similar to PIR|T06827|T06827 lipoxygenase (EC 1.13.11.12...    28  2.9
TC89693 weakly similar to GP|21104633|dbj|BAB93225. hypothetical...    27  5.0
TC82903 similar to GP|21593294|gb|AAM65243.1 putative carboxyl-t...    27  5.0
TC85600 homologue to SP|P29828|PDI_MEDSA Protein disulfide isome...    26  8.5

>TC88736 homologue to GP|13277515|gb|AAK16221.1 PII protein {Medicago
           sativa}, complete
          Length = 733

 Score =  380 bits (976), Expect = e-106
 Identities = 194/194 (100%), Positives = 194/194 (100%)
 Frame = +3

Query: 1   MALIAKPNVFNGLNFHINETQFPFSSFSVIRKRFGDSSHRNVVLKSNGNASILPKIRAQN 60
           MALIAKPNVFNGLNFHINETQFPFSSFSVIRKRFGDSSHRNVVLKSNGNASILPKIRAQN
Sbjct: 36  MALIAKPNVFNGLNFHINETQFPFSSFSVIRKRFGDSSHRNVVLKSNGNASILPKIRAQN 215

Query: 61  LPDYVPESKFYKVEAILRPWRIPQVSSGLLKMGIRGVTVSDVKGFGAQGGSKERQGGSEF 120
           LPDYVPESKFYKVEAILRPWRIPQVSSGLLKMGIRGVTVSDVKGFGAQGGSKERQGGSEF
Sbjct: 216 LPDYVPESKFYKVEAILRPWRIPQVSSGLLKMGIRGVTVSDVKGFGAQGGSKERQGGSEF 395

Query: 121 SEDNFVAKVKMEIVVRKDQVEAVINKIMETARTGEIGDGKIFLIPVSDVIRIRTGERGEQ 180
           SEDNFVAKVKMEIVVRKDQVEAVINKIMETARTGEIGDGKIFLIPVSDVIRIRTGERGEQ
Sbjct: 396 SEDNFVAKVKMEIVVRKDQVEAVINKIMETARTGEIGDGKIFLIPVSDVIRIRTGERGEQ 575

Query: 181 AERMAGGLTDALSV 194
           AERMAGGLTDALSV
Sbjct: 576 AERMAGGLTDALSV 617


>BQ144829 
          Length = 320

 Score = 60.1 bits (144), Expect = 5e-10
 Identities = 29/68 (42%), Positives = 40/68 (58%)
 Frame = +2

Query: 63  DYVPESKFYKVEAILRPWRIPQVSSGLLKMGIRGVTVSDVKGFGAQGGSKERQGGSEFSE 122
           D  P  +F++VEAI+RPWR+  V + L + GI  VT + V+G GA GG+K R  G     
Sbjct: 68  DAXPNCEFFRVEAIIRPWRLEPVMAALAEKGIVAVTSTRVRGAGAXGGTKXRYSGKAHXT 247

Query: 123 DNFVAKVK 130
            + V K K
Sbjct: 248 KDLVEKAK 271


>TC85168 similar to PIR|T06827|T06827 lipoxygenase (EC 1.13.11.12) - garden
            pea, partial (98%)
          Length = 2890

 Score = 27.7 bits (60), Expect = 2.9
 Identities = 9/22 (40%), Positives = 15/22 (67%)
 Frame = -2

Query: 69   KFYKVEAILRPWRIPQVSSGLL 90
            +F+K+E++  PW  P +S  LL
Sbjct: 2565 RFFKLESLFLPWIFPSISDSLL 2500


>TC89693 weakly similar to GP|21104633|dbj|BAB93225. hypothetical
           protein~similar to Arabidopsis thaliana chromosome 3
           At3g46550, partial (53%)
          Length = 1346

 Score = 26.9 bits (58), Expect = 5.0
 Identities = 25/72 (34%), Positives = 35/72 (47%), Gaps = 5/72 (6%)
 Frame = -3

Query: 93  GIRGVTVSDVKGFGAQGGSKERQGGSEFSEDNFVAKVKM-----EIVVRKDQVEAVINKI 147
           G+  +  ++V G GA GG +E   G EF+    V +V       E VV +D  E    ++
Sbjct: 438 GVGDINGAEVVG-GAAGGLEE--SGDEFTGGREVLEVGPGEELEEDVVAEDVGEGGGGEV 268

Query: 148 METARTGEIGDG 159
           ME    GEIG G
Sbjct: 267 MEVRVGGEIGVG 232


>TC82903 similar to GP|21593294|gb|AAM65243.1 putative carboxyl-terminal
           peptidase {Arabidopsis thaliana}, partial (88%)
          Length = 1155

 Score = 26.9 bits (58), Expect = 5.0
 Identities = 12/49 (24%), Positives = 22/49 (44%)
 Frame = +2

Query: 32  KRFGDSSHRNVVLKSNGNASILPKIRAQNLPDYVPESKFYKVEAILRPW 80
           KR+G   H++     +    ++ +   Q+   YV   KFY  +A +  W
Sbjct: 347 KRYGRKKHKSFAKPRSAEPDLVNQSGHQHAIAYVEGDKFYGAKATINVW 493


>TC85600 homologue to SP|P29828|PDI_MEDSA Protein disulfide isomerase
            precursor (PDI) (EC 5.3.4.1). [Alfalfa] {Medicago
            sativa}, complete
          Length = 1915

 Score = 26.2 bits (56), Expect = 8.5
 Identities = 14/61 (22%), Positives = 31/61 (49%)
 Frame = +2

Query: 42   VVLKSNGNASILPKIRAQNLPDYVPESKFYKVEAILRPWRIPQVSSGLLKMGIRGVTVSD 101
            ++  ++G     P +    LP ++   K  KVE  ++   IP+ ++  +K+ + G T+ D
Sbjct: 1040 IIQHNDGKKFFKPNLELDQLPTWLKAYKDGKVEPFVKSEPIPETNNEPVKV-VVGQTLED 1216

Query: 102  V 102
            +
Sbjct: 1217 I 1219


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.318    0.137    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,398,901
Number of Sequences: 36976
Number of extensions: 48474
Number of successful extensions: 258
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 257
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 258
length of query: 194
length of database: 9,014,727
effective HSP length: 91
effective length of query: 103
effective length of database: 5,649,911
effective search space: 581940833
effective search space used: 581940833
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)