BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135464.8 - phase: 0
(559 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC84034 68 9e-23
BQ146262 78 8e-15
BG645818 PIR|S58801|S58 ubiquinol--cytochrome-c reductase (EC 1.... 72 4e-13
TC81386 similar to GP|10140689|gb|AAG13524.1 putative non-LTR re... 63 3e-10
BG583244 40 0.003
BG586283 similar to GP|7267666|em RNA-directed DNA polymerase-li... 32 0.49
TC89641 similar to GP|9988432|dbj|BAB12698.1 hypothetical protei... 32 0.49
TC90111 similar to GP|6728991|gb|AAF26989.1| unknown protein {Ar... 31 1.4
TC84083 30 1.9
AJ498223 weakly similar to PIR|B96556|B965 hypothetical protein ... 30 1.9
CB893216 similar to SP|Q9LPS9|PIR1 Pirin-like protein At1g50590.... 30 3.2
BG452488 30 3.2
BG453817 28 7.1
BE319298 similar to GP|17385689|dbj putative Myb-related transcr... 28 7.1
TC83042 weakly similar to PIR|C86308|C86308 hypothetical protein... 28 7.1
TC78175 similar to GP|1527001|gb|AAB07724.1| Ipomoea nil Pn47p, ... 28 9.3
>TC84034
Length = 485
Score = 67.8 bits (164), Expect(2) = 9e-23
Identities = 31/54 (57%), Positives = 37/54 (68%)
Frame = -2
Query: 35 LLRLFEWSKDFTARTQRQTHAQVWVRLHELPQEYWMDRTLKEIASAIGTPLLID 88
+LR WS+ F + Q +THAQ+WVRL PQEYW RTL EIA +GTPL ID
Sbjct: 367 ILRFSCWSRGFKPQAQVRTHAQIWVRLMHFPQEYWRKRTLFEIAYGLGTPLAID 206
Score = 57.4 bits (137), Expect(2) = 9e-23
Identities = 25/50 (50%), Positives = 37/50 (74%)
Frame = -1
Query: 91 TQNRVFGHYARVLVDMDLSKHIFNEVMIERTGFSFSIEITYERLPAFCTH 140
T +R FG +ARVLVD+DLS+ +F V++E G++ SI+I YE+ P FC +
Sbjct: 200 TLSRRFGVFARVLVDVDLSEQLFESVVVETEGYALSIDIQYEKQPPFCVN 51
>BQ146262
Length = 687
Score = 78.2 bits (191), Expect = 8e-15
Identities = 36/96 (37%), Positives = 58/96 (59%), Gaps = 1/96 (1%)
Frame = -3
Query: 84 PLLIDSATQNRVFGHYARVLVDMDLSKHIFNEVMIERTGFSFSIEITYERLPAFCTHCGN 143
PL ID A ++ +FGHY +L+D+D+S +F+ V++ER GF F + + YER P+FC +C
Sbjct: 619 PLTIDEAIRSXLFGHYVGILMDVDMSSKLFDSVVVEREGFVFPVAVEYERKPSFCYNCKL 440
Query: 144 IGHHNSSCRWLHPVKEPLIDK-KKKTIISQKPQPPK 178
+GH C ++ ++ K ++K I Q P K
Sbjct: 439 LGHSIQLCNRINNIQHHAAPKNQQKKPIQQSNMPNK 332
>BG645818 PIR|S58801|S58 ubiquinol--cytochrome-c reductase (EC 1.10.2.2)
cytochrome b - Leptothorax acervorum mitochondrion,
partial (10%)
Length = 701
Score = 72.4 bits (176), Expect = 4e-13
Identities = 35/72 (48%), Positives = 50/72 (68%)
Frame = +2
Query: 84 PLLIDSATQNRVFGHYARVLVDMDLSKHIFNEVMIERTGFSFSIEITYERLPAFCTHCGN 143
PL ID AT +R FG +ARVLVD+DLS+ +F V++E G+ SI+I YE+ P F +C
Sbjct: 26 PLAIDDATLSRRFGVFARVLVDVDLSEQLFESVVVEIEGYILSIDIQYEK*PPF*VNCKV 205
Query: 144 IGHHNSSCRWLH 155
+GH+ +CR L+
Sbjct: 206 LGHNLQNCRKLN 241
>TC81386 similar to GP|10140689|gb|AAG13524.1 putative non-LTR retroelement
reverse transcriptase {Oryza sativa (japonica
cultivar-group)}, partial (1%)
Length = 798
Score = 63.2 bits (152), Expect = 3e-10
Identities = 28/60 (46%), Positives = 36/60 (59%)
Frame = -1
Query: 480 KRRQLWADLTHIQEHFVGPWLFVGDFNAVLGAHEKCGRRPPPPLSCLDFLNWSNANYLLH 539
KRRQLW+ +++IQ W +GDFN +LG+HE G P L LDF WS+ N L H
Sbjct: 798 KRRQLWSAISNIQTQHKLSWCCIGDFNTILGSHEHQGSHTPARLPMLDFQQWSDVNNLFH 619
>BG583244
Length = 633
Score = 39.7 bits (91), Expect = 0.003
Identities = 24/92 (26%), Positives = 49/92 (53%), Gaps = 2/92 (2%)
Frame = -1
Query: 57 VWVRLHELPQEYWMDRTLKE-IASAIGTPLLIDSATQNRVFGHYARVLVDMDLSKHIFNE 115
+WV+ H+LP + MD T+ + S I ++ D + Y ++ V++++ + + +
Sbjct: 279 IWVQDHQLPFGF-MDTTIGALVGSHIC*MVIFDEENNYGPWRKYVKIRVEIEIEQPLKQD 103
Query: 116 VMIERTGF-SFSIEITYERLPAFCTHCGNIGH 146
+++E + + YE+L FC CG+IGH
Sbjct: 102 LILEGDMCKNIWLVFKYEKLDKFCFVCGSIGH 7
>BG586283 similar to GP|7267666|em RNA-directed DNA polymerase-like protein
{Arabidopsis thaliana}, partial (7%)
Length = 774
Score = 32.3 bits (72), Expect = 0.49
Identities = 15/36 (41%), Positives = 19/36 (52%)
Frame = +3
Query: 481 RRQLWADLTHIQEHFVGPWLFVGDFNAVLGAHEKCG 516
R + W +L+ I G WL GDFN +L EK G
Sbjct: 540 RAKFWDELSTIGAQRDGAWLLTGDFNDLLDNSEKVG 647
>TC89641 similar to GP|9988432|dbj|BAB12698.1 hypothetical protein {Oryza
sativa (japonica cultivar-group)}, partial (44%)
Length = 1080
Score = 32.3 bits (72), Expect = 0.49
Identities = 10/26 (38%), Positives = 19/26 (72%)
Frame = +3
Query: 129 ITYERLPAFCTHCGNIGHHNSSCRWL 154
++ +++ C+HCGNIGH++ +C L
Sbjct: 69 VSVQKMGRKCSHCGNIGHNSRTCNSL 146
>TC90111 similar to GP|6728991|gb|AAF26989.1| unknown protein {Arabidopsis
thaliana}, partial (74%)
Length = 1027
Score = 30.8 bits (68), Expect = 1.4
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = -2
Query: 434 PNLWALWSKDLSITVLFVSDQCIALQISCHQSIVYIAAVYASTYYLK 480
P + LWS S+ LFV QC +SC+ S + + + Y+LK
Sbjct: 402 PPV*CLWS---SVKNLFVETQCCMTALSCNLSFTHFKIISNNKYHLK 271
>TC84083
Length = 641
Score = 30.4 bits (67), Expect = 1.9
Identities = 32/111 (28%), Positives = 44/111 (38%), Gaps = 13/111 (11%)
Frame = +1
Query: 229 QHSDSANVSAAIHHSDDEDNLSAAAHHSDDVSPQNVTKDP-LDFEFIP---VMPD----D 280
+H DS+ HH E H DD P + D +D +++P +M D D
Sbjct: 82 EHMDSSTFDHHHHHQVIEPYSFTMVDHVDDYDPYGIIMDDHVDVDYVPYGIIMDDHVEYD 261
Query: 281 INKATLPSTSIH-VLEE----TADTAEVPIEDTTAAAEPLFTDVMALEAQT 326
TL STS + L E T D + PI++ T L L QT
Sbjct: 262 YEFNTLLSTSENSTLSEISIFTNDHIQFPIDEETLELPSLMELDSILSPQT 414
>AJ498223 weakly similar to PIR|B96556|B965 hypothetical protein F19C24.7
[imported] - Arabidopsis thaliana, partial (11%)
Length = 684
Score = 30.4 bits (67), Expect = 1.9
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -3
Query: 20 MRSVWSKGTQNLKPGLLRLFEWSKDFTART 49
++S W G N+ L+R F W+K + RT
Sbjct: 133 LKSKWKSGKMNISSSLMRCFFWTKTSSTRT 44
>CB893216 similar to SP|Q9LPS9|PIR1 Pirin-like protein At1g50590. [Mouse-ear
cress] {Arabidopsis thaliana}, partial (61%)
Length = 824
Score = 29.6 bits (65), Expect = 3.2
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +1
Query: 345 HHEIPISKNVQHDLDLWARIREYDQRMAEEGFTQVLSK 382
H E+P S+ +Q L LW + +M E + ++LSK
Sbjct: 124 HSEMPASQGIQKGLQLWINLAS-QHKMIEPKYQEILSK 234
>BG452488
Length = 643
Score = 29.6 bits (65), Expect = 3.2
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = +1
Query: 122 GFSFSIEITYERLPAFCTHCGNIGHHNSSC 151
G + YE+ FC CG IGH + SC
Sbjct: 22 GAVIRVNFRYEKPGNFCYECGLIGHTDGSC 111
>BG453817
Length = 668
Score = 28.5 bits (62), Expect = 7.1
Identities = 35/163 (21%), Positives = 60/163 (36%), Gaps = 17/163 (10%)
Frame = +2
Query: 224 ESADAQHSDSANVSAAIHHSDDEDNLSAAAHHSDDVS---PQNVT-------KDPLDFEF 273
ESA A HS S V D N+ H D PQ+V+ + +F F
Sbjct: 50 ESAHAIHSTSNEVEVLPEKEDVNVNIDPLPVHDDKFDAAYPQSVSLKHEEHVTEENNFHF 229
Query: 274 IPVMPDDINKATLPSTSIHVLEETADTAEVPIEDTTAAAEPLFTDVMALEAQTQTNRTDV 333
+ N S+ +HV++ T + ED ++V +E T+T +
Sbjct: 230 NTSQLSERNGVL--SSEMHVMDNDTKTENIHAEDC--------SEVSLVELTTETYQISH 379
Query: 334 ELIAARVASLQHHEIP-------ISKNVQHDLDLWARIREYDQ 369
E+ + + ++ P I +N Q + L E+ +
Sbjct: 380 EIGVSTKTEMDENDFPEEHEPDEIHENSQPESSLMVSANEFQR 508
>BE319298 similar to GP|17385689|dbj putative Myb-related transcription
factor {Oryza sativa (japonica cultivar-group)}, partial
(20%)
Length = 425
Score = 28.5 bits (62), Expect = 7.1
Identities = 9/27 (33%), Positives = 18/27 (66%)
Frame = +2
Query: 125 FSIEITYERLPAFCTHCGNIGHHNSSC 151
F +++ R+ C++CGN GH++ +C
Sbjct: 2 FFVKL*EMRIARKCSYCGNFGHNSRTC 82
>TC83042 weakly similar to PIR|C86308|C86308 hypothetical protein AAD50012.1
[imported] - Arabidopsis thaliana, partial (13%)
Length = 787
Score = 28.5 bits (62), Expect = 7.1
Identities = 15/61 (24%), Positives = 27/61 (43%)
Frame = +1
Query: 225 SADAQHSDSANVSAAIHHSDDEDNLSAAAHHSDDVSPQNVTKDPLDFEFIPVMPDDINKA 284
+A+ H S + H+ ++ H SDD S ++V P DF + D+N+
Sbjct: 499 NAEKDHVTSMPFTRESTHASSVIAMNGRYHSSDDESMESVENSPADFNEVNFPSVDLNET 678
Query: 285 T 285
+
Sbjct: 679 S 681
>TC78175 similar to GP|1527001|gb|AAB07724.1| Ipomoea nil Pn47p, partial
(32%)
Length = 1153
Score = 28.1 bits (61), Expect = 9.3
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +1
Query: 5 LGNGYFEFYFSSYEDMRSVWSKGTQNLKPGLLRL 38
+GN FE FS D+R+++ + ++ P LRL
Sbjct: 874 VGNSNFEKIFSDNNDLRALFIRNNNDIVPSSLRL 975
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.134 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,606,472
Number of Sequences: 36976
Number of extensions: 302379
Number of successful extensions: 1632
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1622
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1632
length of query: 559
length of database: 9,014,727
effective HSP length: 101
effective length of query: 458
effective length of database: 5,280,151
effective search space: 2418309158
effective search space used: 2418309158
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC135464.8