BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135464.2 - phase: 0 /pseudo
(1353 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC89912 weakly similar to PIR|B84512|B84512 probable retroelemen... 90 7e-18
AJ502495 weakly similar to GP|18071369|g putative gag-pol polypr... 82 2e-15
TC85125 weakly similar to SP|P10978|POLX_TOBAC Retrovirus-relate... 54 1e-14
BG647824 weakly similar to PIR|G96722|G96 hypothetical protein F... 60 1e-13
AW691018 weakly similar to GP|2462935|em open reading frame 1 {B... 75 2e-13
BG586159 weakly similar to PIR|T47841|T4 hypothetical protein T2... 75 2e-13
BF649643 similar to PIR|H86486|H86 protein Ty1/copia-element pol... 75 2e-13
AJ497569 weakly similar to PIR|T04833|T04 hypothetical protein F... 49 3e-11
BI309716 weakly similar to PIR|G96722|G9 hypothetical protein F2... 54 4e-07
TC93418 37 6e-07
BF650113 weakly similar to GP|4753889|emb| Tpv2-1c {Phaseolus vu... 51 3e-06
BG644677 weakly similar to GP|7682800|gb| Hypothetical protein T... 47 4e-05
BQ149380 45 2e-04
BF645022 weakly similar to GP|8777581|dbj retroelement pol polyp... 40 0.005
TC83624 homologue to PIR|G84581|G84581 copia-like retroelement p... 39 0.014
TC81230 39 0.014
TC87476 homologue to PIR|T11622|T11622 extensin class 1 precurso... 32 0.017
BG453259 homologue to GP|21434|emb|CA ORF4 {Solanum tuberosum}, ... 36 0.088
TC88329 similar to PIR|T47827|T47827 squamosa promoter binding p... 35 0.20
BE941052 weakly similar to PIR|B85188|B85 retrotransposon like p... 35 0.20
>TC89912 weakly similar to PIR|B84512|B84512 probable retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(10%)
Length = 814
Score = 89.7 bits (221), Expect = 7e-18
Identities = 44/105 (41%), Positives = 72/105 (67%)
Frame = +1
Query: 1195 LLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCEL 1254
L GY DAD+ G DTR+S++G+ F + + +SWK+ +Q +V+ S+++AEY A V +
Sbjct: 88 LEGYVDADYAGNVDTRKSLSGFVFTLYGTTISWKANQQSVVTLSTTQAEYIAFVEGVKDA 267
Query: 1255 Q*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDI 1299
L ++ +L +T V + CDSQSA+H+A++ ++HERTKH+DI
Sbjct: 268 IWLKGMIGELGITQEYVK-IHCDSQSAIHLANHQVYHERTKHIDI 399
>AJ502495 weakly similar to GP|18071369|g putative gag-pol polyprotein {Oryza
sativa}, partial (9%)
Length = 542
Score = 81.6 bits (200), Expect = 2e-15
Identities = 38/99 (38%), Positives = 63/99 (63%)
Frame = +2
Query: 1201 ADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCELQ*LVYL 1260
+DW G +TR+S +GY F +G +SW SKKQ +V+ S++EAEY A + + L +
Sbjct: 2 SDWAGDTETRKSTSGYAFHLGTGAISWSSKKQPVVAFSTAEAEYIASTSCATQTVWLRRI 181
Query: 1261 LTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDI 1299
L ++ + + CD++SA+ ++ NP+FH R+KH+DI
Sbjct: 182 LEVMHHEQNTPTKIYCDNKSAIALSKNPVFHGRSKHIDI 298
>TC85125 weakly similar to SP|P10978|POLX_TOBAC Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
(EC 3.4.23.-);, partial (7%)
Length = 705
Score = 53.9 bits (128), Expect(2) = 1e-14
Identities = 27/56 (48%), Positives = 36/56 (64%)
Frame = +1
Query: 1191 SSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRA 1246
S L + GY D+D+ G D R+S TGY F + VSW SK Q +V+ S++EAEY A
Sbjct: 61 SELTVRGYVDSDFAGDHDKRKSTTGYVFTLAGGAVSWLSKLQTVVALSTTEAEYMA 228
Score = 45.1 bits (105), Expect(2) = 1e-14
Identities = 19/24 (79%), Positives = 20/24 (83%)
Frame = +3
Query: 1276 CDSQSALHIASNPIFHERTKHLDI 1299
CDSQSALHIA NP FH RTKH+ I
Sbjct: 315 CDSQSALHIARNPAFHSRTKHIGI 386
>BG647824 weakly similar to PIR|G96722|G96 hypothetical protein F20P5.25
[imported] - Arabidopsis thaliana, partial (5%)
Length = 721
Score = 60.1 bits (144), Expect(2) = 1e-13
Identities = 30/59 (50%), Positives = 41/59 (68%), Gaps = 1/59 (1%)
Frame = -3
Query: 1244 YRALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSAL-HIASNPIFHERTKHLDIDC 1301
YR++ ++ CE++ L YLL DL T K L CD+QSA HIA+N F ERTKH+++DC
Sbjct: 374 YRSI*STICEIKWLTYLLNDLKFTFIKPAMLYCDNQSAARHIAANSSFLERTKHIELDC 198
Score = 35.8 bits (81), Expect(2) = 1e-13
Identities = 15/33 (45%), Positives = 23/33 (69%)
Frame = -2
Query: 1202 DWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQI 1234
D C DT +SI+ +C F+G SL+ WKS K+++
Sbjct: 486 D*SSCLDT*KSISYFCIFLGDSLICWKS*KKKV 388
>AW691018 weakly similar to GP|2462935|em open reading frame 1 {Brassica
oleracea}, partial (12%)
Length = 639
Score = 75.1 bits (183), Expect = 2e-13
Identities = 32/73 (43%), Positives = 53/73 (71%)
Frame = +2
Query: 2 RCNNLVHSWLMNSVTPSITQSLIYTDSAVDAWNDLQARFSRADRVRISTLQRDLHSFRQN 61
RCN+LV +W+++S+ P I +S+I++D+A W+DL RFS+ D RI +++++ RQ
Sbjct: 341 RCNDLVLTWILHSIEPDIARSVIFSDTAAAVWSDLHDRFSQGDESRIYQIRQEISECRQG 520
Query: 62 SLSIIEYFTELKS 74
SL I +Y+T+LKS
Sbjct: 521 SLLISDYYTKLKS 559
>BG586159 weakly similar to PIR|T47841|T4 hypothetical protein T2O9.150 -
Arabidopsis thaliana, partial (11%)
Length = 732
Score = 74.7 bits (182), Expect = 2e-13
Identities = 37/108 (34%), Positives = 61/108 (56%)
Frame = +1
Query: 1184 GLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAE 1243
G+ + R+ S L Y+D+D+ G D R+S +GY F + VSW SKKQ +V+ S+++AE
Sbjct: 328 GIMYKRNGSEKLEAYTDSDYAGDLDDRKSTSGYVFMLSSGAVSWSSKKQPVVTLSTTKAE 507
Query: 1244 YRALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFH 1291
+ A C+ + +L L T S + CD+ S + ++ NP+ H
Sbjct: 508 FIAAAFCACQSVWMRRVLEKLGYTQSGSITMYCDNNSTIKLSKNPVLH 651
>BF649643 similar to PIR|H86486|H86 protein Ty1/copia-element polyprotein
[imported] - Arabidopsis thaliana, partial (2%)
Length = 608
Score = 74.7 bits (182), Expect = 2e-13
Identities = 31/87 (35%), Positives = 53/87 (60%)
Frame = +1
Query: 2 RCNNLVHSWLMNSVTPSITQSLIYTDSAVDAWNDLQARFSRADRVRISTLQRDLHSFRQN 61
RCN+++ SWL +SV P + + +I+ +A W D + +FS+ + I +Q+ L S Q
Sbjct: 313 RCNSMILSWLTHSVEPDLAKGVIHAKTACQVWEDFKDQFSQKNIPAIYQIQKSLASLSQG 492
Query: 62 SLSIIEYFTELKSYWEELEVFCPIPNC 88
++S YFT++K W+ELE + +P C
Sbjct: 493 TVSASTYFTKIKGLWDELETYRTLPTC 573
>AJ497569 weakly similar to PIR|T04833|T04 hypothetical protein F21P8.50 -
Arabidopsis thaliana, partial (4%)
Length = 723
Score = 49.3 bits (116), Expect(2) = 3e-11
Identities = 32/72 (44%), Positives = 41/72 (56%)
Frame = +3
Query: 1180 SPGTGLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSS 1239
+PG +F S+ H + G CP + R T +CF SL+SWKSKKQ +VSRS
Sbjct: 30 TPGKCIFVSNASNPHF------NRGSCPYSIR*TTEFCFLSS-SLISWKSKKQCVVSRSF 188
Query: 1240 SEAEYRALVAST 1251
SEA RAL +T
Sbjct: 189 SEA**RALANAT 224
Score = 38.5 bits (88), Expect(2) = 3e-11
Identities = 17/31 (54%), Positives = 22/31 (70%)
Frame = +2
Query: 1276 CDSQSALHIASNPIFHERTKHLDIDCQCSEG 1306
CD+ SALHIA+N +FHERT H + D +G
Sbjct: 293 CDNISALHIAANMVFHERT*HRETDPYIVQG 385
>BI309716 weakly similar to PIR|G96722|G9 hypothetical protein F20P5.25
[imported] - Arabidopsis thaliana, partial (10%)
Length = 744
Score = 53.9 bits (128), Expect = 4e-07
Identities = 21/44 (47%), Positives = 31/44 (69%)
Frame = +2
Query: 1178 ETSPGTGLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG 1221
+T+P GLF+ S+L L ++D+DW CP TR+S+TGY F+G
Sbjct: 608 KTAPAKGLFYSATSNLKLSSFADSDWATCPTTRKSVTGYWVFLG 739
>TC93418
Length = 533
Score = 36.6 bits (83), Expect(2) = 6e-07
Identities = 15/22 (68%), Positives = 18/22 (81%)
Frame = +3
Query: 1277 DSQSALHIASNPIFHERTKHLD 1298
D+QSALH+ SN IFHE T H+D
Sbjct: 225 DNQSALHVTSNLIFHEWTNHID 290
Score = 36.2 bits (82), Expect(2) = 6e-07
Identities = 19/50 (38%), Positives = 31/50 (62%)
Frame = +2
Query: 1210 RRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCELQ*LVY 1259
R+ GY F+ +LVS +S KQ +V S+++ E+ + TCEL *L++
Sbjct: 35 RQPTLGYNIFVSENLVS*QSMKQNVVV*SNTKVEFPLMATITCELI*LLH 184
>BF650113 weakly similar to GP|4753889|emb| Tpv2-1c {Phaseolus vulgaris},
partial (13%)
Length = 494
Score = 50.8 bits (120), Expect = 3e-06
Identities = 30/66 (45%), Positives = 38/66 (57%), Gaps = 3/66 (4%)
Frame = +1
Query: 1184 GLFFP---RDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSS 1240
GL FP + L+ YSD+DW G RRS +GY F + +SW +KKQ I + SS
Sbjct: 238 GLLFPYGAKSEVYELICYSDSDWCG---DRRSTSGYVFKFNDAAISWCTKKQPITALSSY 408
Query: 1241 EAEYRA 1246
EAEY A
Sbjct: 409 EAEYIA 426
>BG644677 weakly similar to GP|7682800|gb| Hypothetical protein T15F17.l
{Arabidopsis thaliana}, partial (3%)
Length = 539
Score = 47.4 bits (111), Expect = 4e-05
Identities = 24/65 (36%), Positives = 39/65 (59%)
Frame = -3
Query: 1184 GLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAE 1243
GLF+ +D S L+GY +A + P RS TGY F G +++SW+S K ++ SS+ A
Sbjct: 396 GLFYSKDCSPDLIGYVNA*YLSDPHKARS*TGYIFTCGNTVISWRSTK*STIATSSNHAY 217
Query: 1244 YRALV 1248
+ ++
Sbjct: 216 VQVIL 202
>BQ149380
Length = 419
Score = 44.7 bits (104), Expect = 2e-04
Identities = 15/33 (45%), Positives = 25/33 (75%)
Frame = +3
Query: 71 ELKSYWEELEVFCPIPNCTCNVRCSCEAMRNSR 103
+L+ W+EL+ + P P CTC ++C+C AMRN++
Sbjct: 69 DLRGLWDELDQYRPKPQCTCPIQCTCFAMRNNK 167
>BF645022 weakly similar to GP|8777581|dbj retroelement pol polyprotein-like
{Arabidopsis thaliana}, partial (8%)
Length = 685
Score = 40.4 bits (93), Expect = 0.005
Identities = 18/56 (32%), Positives = 31/56 (55%)
Frame = -2
Query: 5 NLVHSWLMNSVTPSITQSLIYTDSAVDAWNDLQARFSRADRVRISTLQRDLHSFRQ 60
+++ +WL+N++ PS+ +L Y D A W L+ RF + RI L+ L +Q
Sbjct: 186 SMLIAWLLNTIEPSLRSTLSYYDDAESLWTHLKQRFCVVNGARICQLKASLGECKQ 19
>TC83624 homologue to PIR|G84581|G84581 copia-like retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(1%)
Length = 831
Score = 38.9 bits (89), Expect = 0.014
Identities = 16/36 (44%), Positives = 25/36 (69%)
Frame = +2
Query: 1261 LTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKH 1296
L +L V C+++ + C +Q L+IA N ++HERTKH
Sbjct: 443 LRNLQVQCTRLLLIYCVNQITLYIAKNQVYHERTKH 550
>TC81230
Length = 958
Score = 38.9 bits (89), Expect = 0.014
Identities = 19/77 (24%), Positives = 40/77 (51%), Gaps = 1/77 (1%)
Frame = +1
Query: 4 NNLVHSWLMNSVTPSITQSLIYTDSAVDAWNDLQARFSRADRVRISTLQRDLHSFRQNS- 62
N+ + +W N+ PSI ++A + W+ L+ R++ +D L +DL + +Q S
Sbjct: 400 NHQIITWFRNTSIPSIHMQFGRFENAKEVWDHLKQRYTISDLSHQYQLLKDLSNLKQQSG 579
Query: 63 LSIIEYFTELKSYWEEL 79
+ E+ +++ W +L
Sbjct: 580 QPVYEFLAQMEVIWNQL 630
>TC87476 homologue to PIR|T11622|T11622 extensin class 1 precursor - cowpea,
partial (48%)
Length = 939
Score = 32.0 bits (71), Expect(2) = 0.017
Identities = 27/89 (30%), Positives = 37/89 (41%), Gaps = 8/89 (8%)
Frame = +3
Query: 720 LFFHTNLYLLLNLAGSTSHF-----PSHHIPIHHYQHS*PHLLLTYQPQITHHTLLH-LH 773
L H + +LL + S H P H HH PH L T+ + HH L H LH
Sbjct: 168 LLLHQHPHLLHPIFISRHHLHLPLLPHHMFTSHHLHRLHPHHLHTFINLLLHHLLHHRLH 347
Query: 774 LSLTLTLPLNQLCLPP--*ENPPDQKDYP 800
+S + P + PP ++PP P
Sbjct: 348 ISTSHHRPPSPSPPPPYVYKSPPPPSPSP 434
Score = 32.0 bits (71), Expect = 1.7
Identities = 32/110 (29%), Positives = 46/110 (41%), Gaps = 7/110 (6%)
Frame = +3
Query: 736 TSHFPSHHIPIHHYQ---HS*PHL---LLTYQPQITHHTLLHLHLSLTLTLPLNQLCLPP 789
TS HH P+ H+ H PHL L ++ HH L H HL ++ +L L+Q
Sbjct: 18 TSLLLHHHPPLPHHMYISHHHPHLHPPLHHMSIRVHHHPLHHHHLHMSTSLLLHQ----- 182
Query: 790 *ENPPDQKDYPSILWTINVTKLFIRHL-IPLLTLLAIPIFHLFSTSTHFL 838
+P +L I +++ HL +PLL HL H L
Sbjct: 183 ---------HPHLLHPIFISR---HHLHLPLLPHHMFTSHHLHRLHPHHL 296
Score = 25.4 bits (54), Expect(2) = 0.017
Identities = 15/43 (34%), Positives = 24/43 (54%), Gaps = 5/43 (11%)
Frame = +1
Query: 818 PLLTLLAIPIFHLFST-----STHFLSSQSMSPLAMLKLVYMI 855
PLL LL P+ H+F+ H L S+S L +L+L +++
Sbjct: 409 PLLLLLLHPLLHMFTNLLPHHLLHPLHPMSISHLHLLQLHHLL 537
>BG453259 homologue to GP|21434|emb|CA ORF4 {Solanum tuberosum}, partial (5%)
Length = 657
Score = 36.2 bits (82), Expect = 0.088
Identities = 35/124 (28%), Positives = 54/124 (43%), Gaps = 3/124 (2%)
Frame = -2
Query: 1180 SPGTGLF-FPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRS 1238
S GT + F R+ L + Y + G R S +GY F+G ++V K Q +V+R
Sbjct: 548 SSGTQMIVFKRE*KLSMEVYXNTVCAGWIVDRGSTSGY*MFLGGNMVE*K---QNVVAR* 378
Query: 1239 SSEAEYRALVASTCEL--Q*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKH 1296
+ + + L L DL + L ++ IA NP+ H RTKH
Sbjct: 377 VQRHNFELCSQGL*RVMDEELKIKLDDLIINYKDPMTLF*NNNFVSRIAHNPVQHYRTKH 198
Query: 1297 LDID 1300
++ID
Sbjct: 197 IEID 186
>TC88329 similar to PIR|T47827|T47827 squamosa promoter binding protein-like
12 [imported] - Arabidopsis thaliana, partial (19%)
Length = 1277
Score = 35.0 bits (79), Expect = 0.20
Identities = 19/56 (33%), Positives = 30/56 (52%), Gaps = 3/56 (5%)
Frame = +3
Query: 626 WSLMLFLL*IKYLLM---LFKEKFLINLCTMNCLICLVSKYLFHSALLLLKIVINL 678
W + FLL +K +++ L FL+ L CL C++ +LF S LLL +I +
Sbjct: 1068 WVFLFFLLKLKTIMVFTYLLLISFLVRLHVWICLYCILKLHLFKSLLLLYNSIIRV 1235
>BE941052 weakly similar to PIR|B85188|B85 retrotransposon like protein
[imported] - Arabidopsis thaliana, partial (4%)
Length = 480
Score = 35.0 bits (79), Expect = 0.20
Identities = 13/27 (48%), Positives = 18/27 (66%)
Frame = +2
Query: 1274 LLCDSQSALHIASNPIFHERTKHLDID 1300
L CD SA ++ NP++H R KH+ ID
Sbjct: 23 LRCDYLSATYLTHNPVYHSRMKHISID 103
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.360 0.159 0.618
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,321,082
Number of Sequences: 36976
Number of extensions: 1015966
Number of successful extensions: 13929
Number of sequences better than 10.0: 83
Number of HSP's better than 10.0 without gapping: 4477
Number of HSP's successfully gapped in prelim test: 898
Number of HSP's that attempted gapping in prelim test: 8773
Number of HSP's gapped (non-prelim): 6507
length of query: 1353
length of database: 9,014,727
effective HSP length: 108
effective length of query: 1245
effective length of database: 5,021,319
effective search space: 6251542155
effective search space used: 6251542155
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.9 bits)
S2: 64 (29.3 bits)
Medicago: description of AC135464.2