BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135464.1 - phase: 0 /pseudo
(317 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF635063 weakly similar to PIR|F84486|F84 probable retroelement ... 291 2e-79
BG646342 weakly similar to PIR|F84486|F84 probable retroelement ... 128 3e-30
BF648150 similar to GP|14586969|gb| pol polyprotein {Citrus x pa... 53 2e-07
TC93065 45 5e-05
AW736233 GP|21105451|gb small nuclear ribonucleoprotein D1 {Dani... 43 1e-04
TC87868 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 37 0.010
TC87639 GP|9663153|emb|CAC01132.1 transport-secretion protein 2.... 37 0.013
TC87233 similar to PIR|T06048|T06048 kinesin-related protein kat... 36 0.022
TC84935 similar to PIR|G96631|G96631 probable RNA-binding protei... 35 0.050
AJ388976 similar to PIR|E84638|E84 probable RSZp22 splicing fact... 34 0.085
AJ499215 weakly similar to GP|6642775|gb| gag-pol polyprotein {V... 34 0.085
TC83030 weakly similar to GP|18855061|gb|AAL79753.1 putative RNA... 34 0.085
BG450974 similar to PIR|T05112|T05 splicing factor 9G8-like SR p... 33 0.11
TC83119 similar to GP|13357253|gb|AAK20050.1 putative zinc finge... 33 0.14
TC79595 similar to GP|3850823|emb|CAA77136.1 U2 snRNP auxiliary ... 32 0.42
TC78550 weakly similar to GP|21689669|gb|AAM67456.1 unknown prot... 31 0.72
TC82733 similar to GP|10177404|dbj|BAB10535. gene_id:K24M7.12~pi... 30 0.94
TC80683 homologue to GP|8777424|dbj|BAA97014.1 gb|AAF56406.1~gen... 30 0.94
TC91075 similar to PIR|T48025|T48025 hypothetical protein T12C14... 30 1.2
TC92872 29 2.1
>BF635063 weakly similar to PIR|F84486|F84 probable retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(4%)
Length = 677
Score = 291 bits (746), Expect = 2e-79
Identities = 149/189 (78%), Positives = 167/189 (87%)
Frame = -2
Query: 2 MGSKWDIEKFTGSNDFGL*KVKMQAILTQQKCVEALKGEAEMPATLTQEEKREMVDKAKS 61
MGSK DIEKFTG NDFGL KVKM+A+L QQKC +ALKGE +P T+++ EK EMVDKA+S
Sbjct: 568 MGSKRDIEKFTGDNDFGLWKVKMEAVLIQQKCEKALKGEVSLPVTMSRAEKTEMVDKARS 389
Query: 62 AIVLCLGDKVLRDVAREATAASMLAKWESLYMTKSLAHRQLLKQQLYSFKMMESKSISEQ 121
AIVLCLGDKVLR+VA+E TAASM AK SLYMTKSLAHRQ LKQQLYSF+M+ESK+I EQ
Sbjct: 388 AIVLCLGDKVLREVAKERTAASMWAKL*SLYMTKSLAHRQFLKQQLYSFRMVESKAIMEQ 209
Query: 122 LTEFNKILDDLANIEVNMEDEDKALLLLCSLPKSFEHFKDTILYGKEGTATLEEVQAALR 181
LTEFNKILDDL NIEV +EDE+KA+LLLC+LPKSFE FKDT+LYGKEGT TLEEVQAALR
Sbjct: 208 LTEFNKILDDLENIEVQLEDEEKAILLLCALPKSFESFKDTMLYGKEGTVTLEEVQAALR 29
Query: 182 TKELTKFKD 190
TKELTK D
Sbjct: 28 TKELTKSND 2
>BG646342 weakly similar to PIR|F84486|F84 probable retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(4%)
Length = 599
Score = 128 bits (322), Expect = 3e-30
Identities = 68/94 (72%), Positives = 79/94 (83%)
Frame = +2
Query: 59 AKSAIVLCLGDKVLRDVAREATAASMLAKWESLYMTKSLAHRQLLKQQLYSFKMMESKSI 118
A+SAIVLCLGDKVLR+VA+E TA SM AK E LYMTKSLAHRQ LKQQLYSFKM+ESK+I
Sbjct: 2 ARSAIVLCLGDKVLREVAKEPTATSMCAKLEYLYMTKSLAHRQFLKQQLYSFKMVESKAI 181
Query: 119 SEQLTEFNKILDDLANIEVNMEDEDKALLLLCSL 152
+E L EFNKI+ DL NIEV++ED AL++ C L
Sbjct: 182 TELLVEFNKIIGDLENIEVHLEDAG-ALMVWCCL 280
>BF648150 similar to GP|14586969|gb| pol polyprotein {Citrus x paradisi},
partial (3%)
Length = 658
Score = 52.8 bits (125), Expect = 2e-07
Identities = 35/127 (27%), Positives = 64/127 (49%), Gaps = 7/127 (5%)
Frame = +2
Query: 65 LCLG-------DKVLRDVAREATAASMLAKWESLYMTKSLAHRQLLKQQLYSFKMMESKS 117
+CLG D + +A + K E+ YM + ++ L ++KM+++KS
Sbjct: 197 ICLGHILNGMSDSLFDIYQSSPSAKDLWDKLETRYMREDATSKKFLVSHFNNYKMVDNKS 376
Query: 118 ISEQLTEFNKILDDLANIEVNMEDEDKALLLLCSLPKSFEHFKDTILYGKEGTATLEEVQ 177
+ EQL E +IL++ +NM++ ++ LP S++ FK T+ + KE +LE++
Sbjct: 377 VMEQLYEIERILNNYKQHNMNMDETIIVSSIIDKLPPSWKDFKRTMKHKKE-DISLEQLG 553
Query: 178 AALRTKE 184
LR E
Sbjct: 554 NHLRLXE 574
>TC93065
Length = 783
Score = 44.7 bits (104), Expect = 5e-05
Identities = 37/176 (21%), Positives = 80/176 (45%), Gaps = 12/176 (6%)
Frame = +2
Query: 103 LKQQLYSFKMMESKSISEQLTEFNKILDDLANIEVNMEDEDKALLLLCSLPKSFEHFKDT 162
L+++ + KM E++++ E +K++ + + ++ D+ +L LP+ FE +
Sbjct: 215 LRREFEALKMKETETVREFSDRISKVVTQIRLLGEDLSDQRVVEKILVCLPEMFEAKISS 394
Query: 163 ILYGKE-GTATLEEVQAALRTKELTKFKDMKVDEGSEGLNVTRGRNEHRG-KGKGKSRSK 220
+ K T+ E+ AL+ E + + ++++E EG + + +++ K GK +
Sbjct: 395 LEENKNFSEITVAELVNALQASE--QRRSLRMEENVEGAFLANNKGKNQSFKSFGKKKFP 568
Query: 221 -----SRSKGFDKSKY-----KCFLCHKQGHFKKDCPDKGGDGSPSVQVAEASNEE 266
+ DK + KC C++ GH +K C +K +V E E+
Sbjct: 569 PCPHCKKDTHLDKFCWYRPGVKCRACNQLGHVEKVCKNKTNQQEQEARVVEHHQED 736
>AW736233 GP|21105451|gb small nuclear ribonucleoprotein D1 {Danio rerio},
partial (14%)
Length = 510
Score = 43.1 bits (100), Expect = 1e-04
Identities = 28/102 (27%), Positives = 45/102 (43%), Gaps = 2/102 (1%)
Frame = +1
Query: 196 GSEGLNVTRGRNEHRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQGHFKKDCPDKGGDGSP 255
G+E N RGR RG+G+G+ RS S ++ +C +C + H C
Sbjct: 208 GTEYYNAGRGRGRGRGRGRGRGRSNS-------NRLQCQICARNNHDAARC--------- 339
Query: 256 SVQVAEASNEEGYESA--GALVVTSWEPEKSWVLDSGCSYHM 295
+ +AS+ + + A +S E W DSG S+H+
Sbjct: 340 CFRYDQASSSQAHHRAPPSEYAASSSYSEAPWYPDSGASHHL 465
>TC87868 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana, partial (91%)
Length = 860
Score = 37.0 bits (84), Expect = 0.010
Identities = 20/63 (31%), Positives = 29/63 (45%), Gaps = 3/63 (4%)
Frame = +3
Query: 195 EGSEGLNVTRGRNEHRGKGKGKS---RSKSRSKGFDKSKYKCFLCHKQGHFKKDCPDKGG 251
+G G V N G G G R + G S KC+ C + GHF ++C ++GG
Sbjct: 216 DGKNGWRVELSHNSRSGGGGGGGGGGRGRGGGGG-GGSDLKCYECGEPGHFARECRNRGG 392
Query: 252 DGS 254
G+
Sbjct: 393 GGA 401
>TC87639 GP|9663153|emb|CAC01132.1 transport-secretion protein 2.2 (TTS-2.2)
{Homo sapiens}, partial (2%)
Length = 1522
Score = 36.6 bits (83), Expect = 0.013
Identities = 40/202 (19%), Positives = 84/202 (40%), Gaps = 19/202 (9%)
Frame = -1
Query: 85 LAKWESLYMTKSLAHRQLLKQQLYSFKMMESKSISEQLTEFNKILDD-----------LA 133
+A + Y+ ++ R + QL S + +++ ++ L F K+L D ++
Sbjct: 781 MAYLDRTYLDPNIQSRAVA--QLQSLRQKDTERLATFLPRFEKVLADAGGYSWPDVVQIS 608
Query: 134 NIEVNMEDEDKALLLLCSLPKSFEHFKDTILYGKEGTATLEEVQAA-LRTKELTKFKDMK 192
+E + K LL+ LP + + + ++ +E ++ R T+ +
Sbjct: 607 LLETALVPRLKELLITVELPTVYSQWLSKV---QDIAWKMERMKTPPTRWAPATR---LP 446
Query: 193 VDEGSEGLNVTRGRNEHRGKGKGKSRSKSRSKGFD---KSKYKCFLCHKQGHFKKDCPD- 248
V + +G + G + + +G S S S ++G + +C+ CH++GH ++C +
Sbjct: 445 VSKDRDGDMMMTGAIHKQRRRRGSSSSVSSAEGAPPPRRDMRECYSCHERGHIARNCTNT 266
Query: 249 ---KGGDGSPSVQVAEASNEEG 267
K P V E EG
Sbjct: 265 SAAKKKKKGPKVAKVEPQKAEG 200
>TC87233 similar to PIR|T06048|T06048 kinesin-related protein katB -
Arabidopsis thaliana, partial (18%)
Length = 1488
Score = 35.8 bits (81), Expect = 0.022
Identities = 55/229 (24%), Positives = 94/229 (41%), Gaps = 41/229 (17%)
Frame = +3
Query: 21 KVKMQAILTQQKCVEALKGEAEMPATLTQEEKREMVDKAKSAIVLCLGDKVLRDVAREAT 80
K+K L+QQKCVE E+ + +EE ++ + + L +K++++ +A
Sbjct: 558 KLKSSLELSQQKCVEI-----ELLLKIKEEELNSIITEMRRNCT-SLQEKLVKEETEKAA 719
Query: 81 AASMLAK-------WESLYMTKS----LAHRQL--LKQQLYSFKMMESKSISEQLTEFN- 126
A L K +E T S A R+L Q++ S M K + E +T
Sbjct: 720 AMESLIKEREARLDFERSQTTLSEDLGRAQRELETANQKIVSLNDMY-KRLQEYITSLQQ 896
Query: 127 ---KILDDLANIEVNME--DEDKALL----------LLCSLPKSFEHFKDTILYGKEGTA 171
K+ +L+ +E +++ +++KA + L S+ E K + E T+
Sbjct: 897 YNGKLHSELSTVEGDLKRVEKEKATVVENLTMLKGQLALSMASQEEATKQKDAFASEVTS 1076
Query: 172 TLEEV------------QAALRTKELTKFKDMKVDEGSEGLNVTRGRNE 208
E+ Q + E+ KFKD GSE N+T NE
Sbjct: 1077LRSELHQVRDDRDRQISQVQTLSTEIVKFKDSSEKSGSEVNNLTMKTNE 1223
>TC84935 similar to PIR|G96631|G96631 probable RNA-binding protein F8A5.17
[imported] - Arabidopsis thaliana, partial (41%)
Length = 552
Score = 34.7 bits (78), Expect = 0.050
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = +2
Query: 233 CFLCHKQGHFKKDCPDKGGDG 253
CF C + GH+ +DCP GGDG
Sbjct: 473 CFKCGRPGHWARDCPLAGGDG 535
>AJ388976 similar to PIR|E84638|E84 probable RSZp22 splicing factor
[imported] - Arabidopsis thaliana, partial (62%)
Length = 508
Score = 33.9 bits (76), Expect = 0.085
Identities = 20/52 (38%), Positives = 24/52 (45%), Gaps = 1/52 (1%)
Frame = +2
Query: 203 TRGRNEHRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQGHFKKDC-PDKGGDG 253
T G RG G G + RS G S KC+ C + GHF + C GG G
Sbjct: 257 TGGGGGGRGGGGGGGGGRGRSGG-GGSDLKCYXCGEPGHFARXCNSSPGGSG 409
>AJ499215 weakly similar to GP|6642775|gb| gag-pol polyprotein {Vitis
vinifera}, partial (18%)
Length = 567
Score = 33.9 bits (76), Expect = 0.085
Identities = 14/38 (36%), Positives = 23/38 (59%)
Frame = +3
Query: 280 EPEKSWVLDSGCSYHMCPRKEYFETLILKEGGVVRLGN 317
+P K W++DSGC++HM ++ F+ L VR+ N
Sbjct: 93 QPSKYWLIDSGCTHHMTHDRDLFKELNKSTISKVRMLN 206
>TC83030 weakly similar to GP|18855061|gb|AAL79753.1 putative RNA helicase
{Oryza sativa}, partial (7%)
Length = 624
Score = 33.9 bits (76), Expect = 0.085
Identities = 15/38 (39%), Positives = 19/38 (49%)
Frame = +1
Query: 215 GKSRSKSRSKGFDKSKYKCFLCHKQGHFKKDCPDKGGD 252
G+ S+S S CF C + GH DCP+K GD
Sbjct: 112 GRQSSRSSSSPNRSFAGTCFTCGESGHRASDCPNKRGD 225
>BG450974 similar to PIR|T05112|T05 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana, partial (54%)
Length = 364
Score = 33.5 bits (75), Expect = 0.11
Identities = 16/52 (30%), Positives = 23/52 (43%)
Frame = +1
Query: 195 EGSEGLNVTRGRNEHRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQGHFKKDC 246
+G G V N G G G+ + G D KC+ C + GHF ++C
Sbjct: 214 DGKNGWRVQLSHNSKSGGGGGRGGGRGGRGGDD---LKCYECGEPGHFAREC 360
>TC83119 similar to GP|13357253|gb|AAK20050.1 putative zinc finger protein
{Oryza sativa (japonica cultivar-group)}, partial (16%)
Length = 421
Score = 33.1 bits (74), Expect = 0.14
Identities = 21/79 (26%), Positives = 37/79 (46%), Gaps = 13/79 (16%)
Frame = +3
Query: 183 KELTKFKDMKVDEGSEGLNVTRGRNEHRGKGKG--KSRS-----------KSRSKGFDKS 229
KE+ K K++K+ S + +R R+ R + K RS + S+GF +
Sbjct: 162 KEVKKEKNLKMSSDSRSRSRSRSRSRSRSRSPRIRKIRSDRHSYRDAPYRRDSSRGFSRD 341
Query: 230 KYKCFLCHKQGHFKKDCPD 248
C C + GH+ ++CP+
Sbjct: 342 NL-CKNCKRPGHYARECPN 395
>TC79595 similar to GP|3850823|emb|CAA77136.1 U2 snRNP auxiliary factor
large subunit {Nicotiana plumbaginifolia}, partial (77%)
Length = 1525
Score = 31.6 bits (70), Expect = 0.42
Identities = 14/26 (53%), Positives = 20/26 (76%), Gaps = 1/26 (3%)
Frame = +2
Query: 203 TRGRNEHRGKGKGKSRSKS-RSKGFD 227
+R R+EHR + + +SRSKS R+ GFD
Sbjct: 89 SRARSEHRSRSRSRSRSKSKRTSGFD 166
>TC78550 weakly similar to GP|21689669|gb|AAM67456.1 unknown protein
{Arabidopsis thaliana}, partial (69%)
Length = 1486
Score = 30.8 bits (68), Expect = 0.72
Identities = 20/73 (27%), Positives = 34/73 (46%)
Frame = +1
Query: 89 ESLYMTKSLAHRQLLKQQLYSFKMMESKSISEQLTEFNKILDDLANIEVNMEDEDKALLL 148
ES + L+ +L K+QL ++ME + + DL N ++ED +ALL
Sbjct: 289 ESAQSHQKLSPSELQKRQLEIKEIMEKTKMLSDAELMKIAIKDLNNASTSLEDRYRALLE 468
Query: 149 LCSLPKSFEHFKD 161
L L + ++ D
Sbjct: 469 LLELVEPLDNAND 507
>TC82733 similar to GP|10177404|dbj|BAB10535.
gene_id:K24M7.12~pir||S42136~similar to unknown protein
{Arabidopsis thaliana}, partial (57%)
Length = 710
Score = 30.4 bits (67), Expect = 0.94
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +3
Query: 232 KCFLCHKQGHFKKDCP 247
+CF+C +QGH K+CP
Sbjct: 510 QCFVCKEQGHLSKNCP 557
Score = 28.9 bits (63), Expect = 2.7
Identities = 23/80 (28%), Positives = 34/80 (41%), Gaps = 12/80 (15%)
Frame = +3
Query: 183 KELTKFKDMKVDEGSEGLNVTRGRNEHRGKGKGKSRSKSRSKGFD--------KSKYK-- 232
K+ KFK K D S+ G+ R G S KG D K++++
Sbjct: 180 KKKNKFKRKKPDSNSKPRT---GKRPLRVPGMKPGDSCFICKGLDHIAKFCTQKAEWEKN 350
Query: 233 --CFLCHKQGHFKKDCPDKG 250
C C ++GH ++CPD G
Sbjct: 351 KICLRCRRRGHRAQNCPDGG 410
>TC80683 homologue to GP|8777424|dbj|BAA97014.1
gb|AAF56406.1~gene_id:K9P8.7~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (16%)
Length = 1360
Score = 30.4 bits (67), Expect = 0.94
Identities = 22/89 (24%), Positives = 34/89 (37%), Gaps = 10/89 (11%)
Frame = +1
Query: 184 ELTKFKDMKVDEGSEGLNVTRGRNEHRGKGKGKSRSKSRSKGFDKSKYK----------C 233
+L K K+ D+ E ++ GK K + + DK K C
Sbjct: 901 KLKKMKEKYADQDEEERSIRMSLLASSGKPIKKEETLPVIETSDKGKKSDSGPIDAPKIC 1080
Query: 234 FLCHKQGHFKKDCPDKGGDGSPSVQVAEA 262
+ C K GH +DC ++ D S +EA
Sbjct: 1081YKCKKVGHLSRDCKEQPNDLLHSHATSEA 1167
>TC91075 similar to PIR|T48025|T48025 hypothetical protein T12C14.30 -
Arabidopsis thaliana, partial (29%)
Length = 686
Score = 30.0 bits (66), Expect = 1.2
Identities = 14/47 (29%), Positives = 26/47 (54%), Gaps = 3/47 (6%)
Frame = +2
Query: 216 KSRSKSRSKGFDKSKYK---CFLCHKQGHFKKDCPDKGGDGSPSVQV 259
+++SK+ + + K K + C LC + GHF + CP G + S ++
Sbjct: 329 EAKSKATERNWKKRKEEEMICKLCGESGHFTQGCPSTLGANNKSQEL 469
>TC92872
Length = 923
Score = 29.3 bits (64), Expect = 2.1
Identities = 20/73 (27%), Positives = 36/73 (48%)
Frame = +1
Query: 80 TAASMLAKWESLYMTKSLAHRQLLKQQLYSFKMMESKSISEQLTEFNKILDDLANIEVNM 139
TA ++ ES + S +H + Q+ + KSI++ L + D+LA I+ ++
Sbjct: 346 TARTLWVAIESQFNNTSRSHVMSVTNQIQRCTKGD-KSITDYLFSVKSLADELAVIDKSL 522
Query: 140 EDEDKALLLLCSL 152
D+D L +L L
Sbjct: 523 SDDDITLFVLNGL 561
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.132 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,033,359
Number of Sequences: 36976
Number of extensions: 109344
Number of successful extensions: 833
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 737
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 807
length of query: 317
length of database: 9,014,727
effective HSP length: 96
effective length of query: 221
effective length of database: 5,465,031
effective search space: 1207771851
effective search space used: 1207771851
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC135464.1