BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135461.14 - phase: 0 /pseudo
(1361 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC82780 similar to PIR|T00713|T00713 helicase homolog F22O13.8 -... 359 5e-99
BQ140584 similar to PIR|F86218|F8 protein F22O13.8 [imported] - ... 230 3e-60
BE205322 similar to PIR|F86218|F86 protein F22O13.8 [imported] -... 129 6e-48
TC79652 weakly similar to GP|19698951|gb|AAL91211.1 unknown prot... 36 0.089
TC92278 similar to SP|O43093|KINH_SYNRA Kinesin heavy chain (Syn... 33 0.98
TC76790 similar to PIR|T06817|T06817 RNA-binding protein - garde... 31 3.7
TC79207 similar to GP|10086489|gb|AAG12549.1 Unknown Protein {Ar... 30 4.9
>TC82780 similar to PIR|T00713|T00713 helicase homolog F22O13.8 - Arabidopsis
thaliana, partial (5%)
Length = 563
Score = 359 bits (921), Expect = 5e-99
Identities = 187/187 (100%), Positives = 187/187 (100%)
Frame = +1
Query: 1167 *SGR*TTGA*DHI*RRNAASL*IW*RRES*DFG*TK*TK*SDFGWGFTETYSTTFKWKQL 1226
*SGR*TTGA*DHI*RRNAASL*IW*RRES*DFG*TK*TK*SDFGWGFTETYSTTFKWKQL
Sbjct: 1 *SGR*TTGA*DHI*RRNAASL*IW*RRES*DFG*TK*TK*SDFGWGFTETYSTTFKWKQL 180
Query: 1227 F**VNGKLTRQASSPVDCQLSFT*IPVTRKRRGKTIKRGARYGLGGVSQIAGMGRSSEST 1286
F**VNGKLTRQASSPVDCQLSFT*IPVTRKRRGKTIKRGARYGLGGVSQIAGMGRSSEST
Sbjct: 181 F**VNGKLTRQASSPVDCQLSFT*IPVTRKRRGKTIKRGARYGLGGVSQIAGMGRSSEST 360
Query: 1287 DW*VCA*SKARSVKGRRRTT*SRNLAAFN*AEKSFHKAAMHQPCTFADT*KPENKNWGLY 1346
DW*VCA*SKARSVKGRRRTT*SRNLAAFN*AEKSFHKAAMHQPCTFADT*KPENKNWGLY
Sbjct: 361 DW*VCA*SKARSVKGRRRTT*SRNLAAFN*AEKSFHKAAMHQPCTFADT*KPENKNWGLY 540
Query: 1347 SLRGMCP 1353
SLRGMCP
Sbjct: 541 SLRGMCP 561
>BQ140584 similar to PIR|F86218|F8 protein F22O13.8 [imported] - Arabidopsis
thaliana, partial (7%)
Length = 515
Score = 230 bits (586), Expect = 3e-60
Identities = 127/187 (67%), Positives = 135/187 (71%)
Frame = +3
Query: 196 KKISPETLEPGKKETEGDWDVFNKIISDESGIDASFGSKTWASVYLASTPQQAALIGLEF 255
KKISPETLEPGKKETEGDWDVFNKIISDESGIDASFGSKTWASVYLASTPQQAALIGLEF
Sbjct: 54 KKISPETLEPGKKETEGDWDVFNKIISDESGIDASFGSKTWASVYLASTPQQAALIGLEF 233
Query: 256 PGVNEVEEIDDIDGNSTDPFVAAAIANEKELDLSEEQRRQFKKLSPIQLSIYGTIFLRIP 315
PGVNEVEEIDDIDGNSTDPFVAAAIANEKELDLSEEQRRQFKK+
Sbjct: 234 PGVNEVEEIDDIDGNSTDPFVAAAIANEKELDLSEEQRRQFKKVK--------------E 371
Query: 316 NSSAVCSSAFSISLLYSQRRG*CNC**EASSTFETQKTSKKK*TARNKYTYPVDGEPCTE 375
A+ + L + + R K SK++ T+ YPVDGEPCTE
Sbjct: 372 EDDAIVDRKLQVRLKHXRHR----------------KKSKQRETST---PYPVDGEPCTE 494
Query: 376 VFIC*SI 382
VFIC*SI
Sbjct: 495 VFIC*SI 515
Score = 45.4 bits (106), Expect = 1e-04
Identities = 26/37 (70%), Positives = 28/37 (75%), Gaps = 2/37 (5%)
Frame = +1
Query: 178 VRRRHGKLLEEGASGFLQKK--ISPETLEPGKKETEG 212
VRRRHGKLLEEGASGFLQKK + P LE KK+ G
Sbjct: 1 VRRRHGKLLEEGASGFLQKKFLLKPWNLE--KKKLRG 105
>BE205322 similar to PIR|F86218|F86 protein F22O13.8 [imported] - Arabidopsis
thaliana, partial (5%)
Length = 456
Score = 129 bits (325), Expect(2) = 6e-48
Identities = 69/71 (97%), Positives = 69/71 (97%)
Frame = +2
Query: 40 ILSLISELLEVESKAAEAQETLEEESLAKVESEVRQELEQTLQGDDLETAVADEMAAFKE 99
I LISELLEVESKAAEAQETLEEESLAKVESEVRQELEQTLQGDDLETAVADEMAAFKE
Sbjct: 242 IEDLISELLEVESKAAEAQETLEEESLAKVESEVRQELEQTLQGDDLETAVADEMAAFKE 421
Query: 100 EWEAALDDLET 110
EWEAALDDLET
Sbjct: 422 EWEAALDDLET 454
Score = 81.3 bits (199), Expect(2) = 6e-48
Identities = 40/41 (97%), Positives = 40/41 (97%)
Frame = +3
Query: 1 IWREYLRMKW*ILRVLLVVLQMTTQTMRDLRLLILMTNCIL 41
IWREYLRMKW*ILRVLLVVLQMTT TMRDLRLLILMTNCIL
Sbjct: 96 IWREYLRMKW*ILRVLLVVLQMTTHTMRDLRLLILMTNCIL 218
>TC79652 weakly similar to GP|19698951|gb|AAL91211.1 unknown protein
{Arabidopsis thaliana}, partial (20%)
Length = 1590
Score = 36.2 bits (82), Expect = 0.089
Identities = 25/82 (30%), Positives = 41/82 (49%), Gaps = 10/82 (12%)
Frame = +2
Query: 48 LEVESKAAEAQETLEEESLAKVESEV---RQELEQ----TLQGDDLETAVADEMAAFKEE 100
LE+ K A E ++ LA E+E+ R L+ T + D+ + +E A+ E
Sbjct: 5 LELRVKTANESEARSQQKLAAAEAEIADMRHNLDDSKRATCKQSDVMRSKNEENEAYLSE 184
Query: 101 WEA---ALDDLETESSHLLEQL 119
E A DD++T++ HLL Q+
Sbjct: 185 IETIGQAYDDMQTQNQHLLHQI 250
>TC92278 similar to SP|O43093|KINH_SYNRA Kinesin heavy chain (Synkin).
{Syncephalastrum racemosum}, partial (10%)
Length = 709
Score = 32.7 bits (73), Expect = 0.98
Identities = 32/153 (20%), Positives = 65/153 (41%), Gaps = 15/153 (9%)
Frame = +3
Query: 4 EYLRMKW*ILRVLLVVLQMTTQTMRDLRLLILMTNCILSLISELLEVESKAAEAQETLEE 63
E+L K L + L L++ + + D + N + + + E+ K EAQ ++
Sbjct: 228 EHLTRKRDELEIRLTTLELEYEELLDKTIAEEEANNNIDITETIAELRGKL-EAQFVAKK 404
Query: 64 ESLAKVESEVRQELEQTLQ---------------GDDLETAVADEMAAFKEEWEAALDDL 108
E K E++QELE+ + +DL+TA+ D+ K + + + D
Sbjct: 405 EVQQKEIDELKQELEKKNEELHKLNSALADLKKVNEDLQTAINDK--EIKSDGQKNVADK 578
Query: 109 ETESSHLLEQLDGAGIELPSLYKLIEREAPDRC 141
E + + + + + + K + R+ +RC
Sbjct: 579 EKDMERIXKTMAQQLADFDVMKKALMRDLQNRC 677
>TC76790 similar to PIR|T06817|T06817 RNA-binding protein - garden pea,
partial (79%)
Length = 1141
Score = 30.8 bits (68), Expect = 3.7
Identities = 39/160 (24%), Positives = 64/160 (39%), Gaps = 8/160 (5%)
Frame = +1
Query: 14 RVLLVVLQMTTQTMRD----LRLLILMTNCILSLISE----LLEVESKAAEAQETLEEES 65
R ++V+ TT ++ + L + +C+LS S+ + SK+ +L+
Sbjct: 4 RASIIVITTTTMSITSTTPLFKSLTMAESCLLSSQSKSKPTFFSLPSKSLNLHLSLKTNR 183
Query: 66 LAKVESEVRQELEQTLQGDDLETAVADEMAAFKEEWEAALDDLETESSHLLEQLDGAGIE 125
+ L +GD L T++ DE A +WE D ETE+ + +G +E
Sbjct: 184 SISISPS---PLFVAQEGDTLTTSL-DEEAGLSLDWEPTADAAETETG-ADDSAEGYFVE 348
Query: 126 LPSLYKLIEREAPDRCCTEAWKKRNHWVGSQATTEIASSV 165
P KL P +E K G T EIA +
Sbjct: 349 PPEDAKLFVGNFPFDVDSE---KLAMLFGQAGTVEIAEVI 459
>TC79207 similar to GP|10086489|gb|AAG12549.1 Unknown Protein {Arabidopsis
thaliana}, partial (20%)
Length = 1719
Score = 30.4 bits (67), Expect = 4.9
Identities = 27/106 (25%), Positives = 48/106 (44%)
Frame = +3
Query: 21 QMTTQTMRDLRLLILMTNCILSLISELLEVESKAAEAQETLEEESLAKVESEVRQELEQT 80
+M+++ +L+L LS+ +E LEV + LE E L +EV+ +L +
Sbjct: 636 KMSSEEFEELKLEKEKVTTDLSICTENLEVAKS-----QLLETEQLL---AEVKSQLASS 791
Query: 81 LQGDDLETAVADEMAAFKEEWEAALDDLETESSHLLEQLDGAGIEL 126
+ + L MA + E + ETE +HL +++ EL
Sbjct: 792 ERSNSLAETQLKCMAESYKSLETRAQEFETELNHLQSKIETLENEL 929
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.362 0.161 0.621
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,279,093
Number of Sequences: 36976
Number of extensions: 610885
Number of successful extensions: 5823
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1677
Number of HSP's successfully gapped in prelim test: 220
Number of HSP's that attempted gapping in prelim test: 4075
Number of HSP's gapped (non-prelim): 2071
length of query: 1361
length of database: 9,014,727
effective HSP length: 108
effective length of query: 1253
effective length of database: 5,021,319
effective search space: 6291712707
effective search space used: 6291712707
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (22.0 bits)
S2: 64 (29.3 bits)
Medicago: description of AC135461.14