BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135320.7 + phase: 0
(190 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78358 similar to PIR|B84728|B84728 probable alanine acetyl tra... 381 e-107
TC84245 weakly similar to PIR|B84728|B84728 probable alanine ace... 286 3e-78
TC79782 weakly similar to GP|11994288|dbj|BAB01471. alanine acet... 230 2e-61
BE942208 similar to GP|21554210|gb| putative alanine acetyl tran... 177 3e-45
AL380752 weakly similar to GP|11994288|dbj alanine acetyl transf... 137 3e-33
BF520937 weakly similar to GP|21554210|gb| putative alanine acet... 123 4e-29
TC89746 similar to GP|21554210|gb|AAM63289.1 putative alanine ac... 117 3e-27
BQ151724 similar to PIR|B84728|B847 probable alanine acetyl tran... 68 2e-12
TC91038 28 2.2
TC89074 similar to GP|19387282|gb|AAL87193.1 putative 62.8 kDa p... 28 2.2
BF635667 weakly similar to PIR|C85359|C853 hypothetical protein ... 28 2.8
TC77067 weakly similar to PIR|C96759|C96759 protein serine carbo... 27 6.3
TC88519 similar to PIR|C86437|C86437 hypothetical protein AAD216... 27 6.3
TC78163 H+-ATPase 26 8.2
>TC78358 similar to PIR|B84728|B84728 probable alanine acetyl transferase
[imported] - Arabidopsis thaliana, partial (30%)
Length = 1418
Score = 381 bits (979), Expect = e-107
Identities = 190/190 (100%), Positives = 190/190 (100%)
Frame = +2
Query: 1 MEITSISSKPGAKEERIDLTQITLRPLNLSDLDDLMIWTTDEKVAKFCSWELYTSKDDGI 60
MEITSISSKPGAKEERIDLTQITLRPLNLSDLDDLMIWTTDEKVAKFCSWELYTSKDDGI
Sbjct: 203 MEITSISSKPGAKEERIDLTQITLRPLNLSDLDDLMIWTTDEKVAKFCSWELYTSKDDGI 382
Query: 61 NFIENIATKFLWCKAICINDRAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATC 120
NFIENIATKFLWCKAICINDRAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATC
Sbjct: 383 NFIENIATKFLWCKAICINDRAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATC 562
Query: 121 VVKQVVKVAFCELSYLERLEALVDVENAGSQRVLEKAGFQKEGVLRKYLVMKGKSRDMII 180
VVKQVVKVAFCELSYLERLEALVDVENAGSQRVLEKAGFQKEGVLRKYLVMKGKSRDMII
Sbjct: 563 VVKQVVKVAFCELSYLERLEALVDVENAGSQRVLEKAGFQKEGVLRKYLVMKGKSRDMII 742
Query: 181 SSVLFTDPQL 190
SSVLFTDPQL
Sbjct: 743 SSVLFTDPQL 772
>TC84245 weakly similar to PIR|B84728|B84728 probable alanine acetyl
transferase [imported] - Arabidopsis thaliana, partial
(54%)
Length = 680
Score = 286 bits (732), Expect = 3e-78
Identities = 147/190 (77%), Positives = 159/190 (83%)
Frame = +3
Query: 1 MEITSISSKPGAKEERIDLTQITLRPLNLSDLDDLMIWTTDEKVAKFCSWELYTSKDDGI 60
ME T ISSKP KEE IDL QITLR NLSDLDD+M+W TDEKVAKFCSWE YTS++DGI
Sbjct: 33 MEETFISSKPCGKEEIIDLNQITLRSFNLSDLDDVMVWHTDEKVAKFCSWEPYTSREDGI 212
Query: 61 NFIENIATKFLWCKAICINDRAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATC 120
NFIE+I +KFL CKAICINDRAIGCV L + P DKSR + AELGY L SKYWGKG+ TC
Sbjct: 213 NFIESIESKFLCCKAICINDRAIGCVLLFT--PEDKSRKQSAELGYNLSSKYWGKGIVTC 386
Query: 121 VVKQVVKVAFCELSYLERLEALVDVENAGSQRVLEKAGFQKEGVLRKYLVMKGKSRDMII 180
VKQ+VK+AF E SYLERLEALVDVEN GSQRVLEKAGFQKEG LRKYLVMKGKSRDMI+
Sbjct: 387 AVKQIVKIAFDEFSYLERLEALVDVENVGSQRVLEKAGFQKEGTLRKYLVMKGKSRDMIM 566
Query: 181 SSVLFTDPQL 190
SVL D QL
Sbjct: 567 FSVLSNDLQL 596
>TC79782 weakly similar to GP|11994288|dbj|BAB01471. alanine acetyl
transferase-like protein {Arabidopsis thaliana}, partial
(23%)
Length = 781
Score = 230 bits (587), Expect = 2e-61
Identities = 116/192 (60%), Positives = 149/192 (77%), Gaps = 2/192 (1%)
Frame = +3
Query: 1 MEITSISSKPGAKEER--IDLTQITLRPLNLSDLDDLMIWTTDEKVAKFCSWELYTSKDD 58
ME T+ SK A EE +DL+QI+LRP++LSDLDD+M+WT+D KVAK+C+WE YT+K+
Sbjct: 132 MEETATYSKSDANEEYSVVDLSQISLRPISLSDLDDVMLWTSDAKVAKYCTWEPYTNKEK 311
Query: 59 GINFIENIATKFLWCKAICINDRAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVA 118
GI+FI+NIA+K LW +AIC+ D+AIGC S D+ R+K A+LGY L S YWGKG+A
Sbjct: 312 GIDFIQNIASKSLWFRAICLRDQAIGCTEFQVCS--DRCRDKSAQLGYSLSSMYWGKGIA 485
Query: 119 TCVVKQVVKVAFCELSYLERLEALVDVENAGSQRVLEKAGFQKEGVLRKYLVMKGKSRDM 178
T VVK+VV AF E YLERLEA VDVEN SQRVLEKAGF++EGVLRKYL KGKS D+
Sbjct: 486 TMVVKKVVDAAFKEFPYLERLEARVDVENVASQRVLEKAGFEREGVLRKYLFFKGKSIDV 665
Query: 179 IISSVLFTDPQL 190
++ S+L ++ +L
Sbjct: 666 VMFSILSSEVKL 701
>BE942208 similar to GP|21554210|gb| putative alanine acetyl transferase
{Arabidopsis thaliana}, partial (42%)
Length = 480
Score = 177 bits (448), Expect = 3e-45
Identities = 91/122 (74%), Positives = 99/122 (80%), Gaps = 2/122 (1%)
Frame = +2
Query: 71 LWCKAICINDRAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATCVVKQVVKV-- 128
L CKAIC ND IGC+ L SSS DKSR KCAE+GYVL SKYWGKG+ATC VKQ+V V
Sbjct: 17 LQCKAICHNDHVIGCIKLFSSSLHDKSRYKCAEIGYVLASKYWGKGIATCAVKQMVMVTF 196
Query: 129 AFCELSYLERLEALVDVENAGSQRVLEKAGFQKEGVLRKYLVMKGKSRDMIISSVLFTDP 188
AF E SYLERLEALVDV+N GSQRVLEK GFQKEGVLRKY+ KGKSRDMI+ S+L TD
Sbjct: 197 AFSEFSYLERLEALVDVDNVGSQRVLEKVGFQKEGVLRKYVFFKGKSRDMIMFSLLSTDI 376
Query: 189 QL 190
QL
Sbjct: 377 QL 382
>AL380752 weakly similar to GP|11994288|dbj alanine acetyl transferase-like
protein {Arabidopsis thaliana}, partial (44%)
Length = 491
Score = 137 bits (345), Expect = 3e-33
Identities = 64/162 (39%), Positives = 104/162 (63%)
Frame = +2
Query: 17 IDLTQITLRPLNLSDLDDLMIWTTDEKVAKFCSWELYTSKDDGINFIENIATKFLWCKAI 76
++L++ITLRP LSD+DD M+ D++V + + + S++ + +I+++ W ++I
Sbjct: 2 MNLSRITLRPFELSDVDDFMLVVGDDRVTNYTRRKSFVSREQALCYIQDVCIPHPWTRSI 181
Query: 77 CINDRAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATCVVKQVVKVAFCELSYL 136
CI+++ IG +S+S + GD AE+GY L + YWG+G+ T VK V F ++ L
Sbjct: 182 CIDNQTIGFISISPAGSGDDDDRCRAEIGYALAANYWGQGICTQAVKVAVSQVFKDIPDL 361
Query: 137 ERLEALVDVENAGSQRVLEKAGFQKEGVLRKYLVMKGKSRDM 178
R++A V +EN SQRVLEK GF +EG+LRKY +KG +D+
Sbjct: 362 VRIQAFVSMENQASQRVLEKVGFLREGILRKYSYVKGIVKDV 487
>BF520937 weakly similar to GP|21554210|gb| putative alanine acetyl
transferase {Arabidopsis thaliana}, partial (41%)
Length = 615
Score = 123 bits (309), Expect = 4e-29
Identities = 70/168 (41%), Positives = 103/168 (60%)
Frame = +1
Query: 20 TQITLRPLNLSDLDDLMIWTTDEKVAKFCSWELYTSKDDGINFIENIATKFLWCKAICIN 79
++ITLRP SD DD+++W +DE+V ++ EL S++ NFI+N + + ++IC+N
Sbjct: 4 SRITLRPFKESDADDVLLWASDERVMQYMRLELCKSREQASNFIKNDCI-YPFQQSICLN 180
Query: 80 DRAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATCVVKQVVKVAFCELSYLERL 139
D +IG V + P ++ K A+LGY +G YWG+GVAT VK ++ F + L RL
Sbjct: 181 DHSIGIVFVL---PYPDAKFK-ADLGYAIGFNYWGQGVATKAVKILLSCVFQDFPDLVRL 348
Query: 140 EALVDVENAGSQRVLEKAGFQKEGVLRKYLVMKGKSRDMIISSVLFTD 187
+A +N SQR+LEK GFQ+EG+ RK + KG D I S L TD
Sbjct: 349 QANTRPDNIASQRLLEKVGFQREGLFRKGIYNKGNVEDFCIFSFLSTD 492
>TC89746 similar to GP|21554210|gb|AAM63289.1 putative alanine acetyl
transferase {Arabidopsis thaliana}, partial (25%)
Length = 988
Score = 117 bits (293), Expect = 3e-27
Identities = 66/168 (39%), Positives = 104/168 (61%)
Frame = +1
Query: 20 TQITLRPLNLSDLDDLMIWTTDEKVAKFCSWELYTSKDDGINFIENIATKFLWCKAICIN 79
++ITLRP SD DD+++W +DE+V ++ +L S++ +NFI+ + + ++IC++
Sbjct: 43 SRITLRPFKESDADDVLLWASDERVMQYTRLKLCKSREQALNFIKKDCI-YPFQQSICLD 219
Query: 80 DRAIGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATCVVKQVVKVAFCELSYLERL 139
D +IG V + P ++ K A+LG+ +G YWG+G+AT +K ++ F E L+RL
Sbjct: 220 DHSIGIVFVL---PISYAKFK-ADLGFAIGFNYWGQGIATKALKILLSRVFQEFPDLQRL 387
Query: 140 EALVDVENAGSQRVLEKAGFQKEGVLRKYLVMKGKSRDMIISSVLFTD 187
+A + V+N SQRVLEK GF +EG+ RK KG D I S L TD
Sbjct: 388 QANILVDNMASQRVLEKVGFHREGLFRKVFHHKGNIVDAYIFSFLSTD 531
>BQ151724 similar to PIR|B84728|B847 probable alanine acetyl transferase
[imported] - Arabidopsis thaliana, partial (7%)
Length = 206
Score = 67.8 bits (164), Expect = 2e-12
Identities = 35/59 (59%), Positives = 40/59 (67%)
Frame = +3
Query: 83 IGCVSLSSSSPGDKSRNKCAELGYVLGSKYWGKGVATCVVKQVVKVAFCELSYLERLEA 141
IGC S D+ R+K A+LGY L S YWGKG+AT VVK+VV AF E YLERLEA
Sbjct: 33 IGCTEFQVCS--DRCRDKSAQLGYSLSSMYWGKGIATMVVKKVVDAAFKEFPYLERLEA 203
>TC91038
Length = 586
Score = 28.1 bits (61), Expect = 2.2
Identities = 19/54 (35%), Positives = 27/54 (49%)
Frame = +1
Query: 9 KPGAKEERIDLTQITLRPLNLSDLDDLMIWTTDEKVAKFCSWELYTSKDDGINF 62
K G+ EERIDL +TLR N+ + M D K +L T D G+++
Sbjct: 64 KRGSVEERIDLIHVTLRVANIGNRTAYM---RDLKEYLEAMDDLQTLLDSGLDY 216
>TC89074 similar to GP|19387282|gb|AAL87193.1 putative 62.8 kDa protein {Oryza
sativa (japonica cultivar-group)}, partial (62%)
Length = 2244
Score = 28.1 bits (61), Expect = 2.2
Identities = 13/36 (36%), Positives = 24/36 (66%)
Frame = -2
Query: 59 GINFIENIATKFLWCKAICINDRAIGCVSLSSSSPG 94
GINF E+ ATK + K++ I+ GC++ ++++ G
Sbjct: 1106 GINF-EDFATKLIIAKSLLIDSPTPGCLTFTATNMG 1002
>BF635667 weakly similar to PIR|C85359|C853 hypothetical protein AT4g30700
[imported] - Arabidopsis thaliana, partial (6%)
Length = 603
Score = 27.7 bits (60), Expect = 2.8
Identities = 20/66 (30%), Positives = 30/66 (45%)
Frame = +3
Query: 113 WGKGVATCVVKQVVKVAFCELSYLERLEALVDVENAGSQRVLEKAGFQKEGVLRKYLVMK 172
WG +A CV+ Q VK+ +L +LE N S V +Q G++ +
Sbjct: 204 WGALLAGCVMHQNVKIGEVAAHHLFQLE-----PNNTSNYVALWGIYQSRGMVLGVSTIX 368
Query: 173 GKSRDM 178
GK RD+
Sbjct: 369 GKMRDL 386
>TC77067 weakly similar to PIR|C96759|C96759 protein serine carboxypeptidase
T18K17.3 [imported] - Arabidopsis thaliana, partial (71%)
Length = 1582
Score = 26.6 bits (57), Expect = 6.3
Identities = 12/28 (42%), Positives = 18/28 (63%)
Frame = +2
Query: 20 TQITLRPLNLSDLDDLMIWTTDEKVAKF 47
TQ +R LN S +DD W T+++VA +
Sbjct: 1250 TQAWIRSLNYSIVDDWRQWHTNDQVAGY 1333
>TC88519 similar to PIR|C86437|C86437 hypothetical protein AAD21696.1
[imported] - Arabidopsis thaliana, partial (65%)
Length = 708
Score = 26.6 bits (57), Expect = 6.3
Identities = 11/28 (39%), Positives = 12/28 (42%)
Frame = -3
Query: 69 KFLWCKAICINDRAIGCVSLSSSSPGDK 96
K+ WC I IN GC S G K
Sbjct: 235 KYCWCSTISINSSCFGCSLFIVCSAGAK 152
>TC78163 H+-ATPase
Length = 3295
Score = 26.2 bits (56), Expect = 8.2
Identities = 26/84 (30%), Positives = 33/84 (38%), Gaps = 17/84 (20%)
Frame = +3
Query: 47 FCSWEL---YTSKDDGINFIENIATKFL----WC-------KAICI--NDRAIGCVSLSS 90
FC W + +D +++ K L WC K C NDR +G LSS
Sbjct: 2280 FCYWGYSWYLSGYNDSHLLLDSYGNKLLS*QFWCAPLPP*SKGSCH**NDREVGFCCLSS 2459
Query: 91 SSPGDKSRNKCAEL-GYVLGSKYW 113
S S N C + G VL K W
Sbjct: 2460 SQYN*SSLNLCDTV*GLVLYRKTW 2531
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.135 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,853,090
Number of Sequences: 36976
Number of extensions: 81900
Number of successful extensions: 342
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 336
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 336
length of query: 190
length of database: 9,014,727
effective HSP length: 91
effective length of query: 99
effective length of database: 5,649,911
effective search space: 559341189
effective search space used: 559341189
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC135320.7