BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135319.9 + phase: 0 /pseudo
(734 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC81680 similar to GP|6729044|gb|AAF27040.1| hypothetical protei... 83 3e-16
BE248562 36 0.061
AW257410 weakly similar to GP|10177533|db selenium-binding prote... 33 0.51
TC81653 similar to SP|P93149|C9B1_GLYEC Cytochrome P450 93B1 (EC... 33 0.51
TC78402 weakly similar to PIR|B84853|B84853 hypothetical protein... 31 1.5
TC86734 similar to PIR|D86257|D86257 hypothetical protein [impor... 30 3.3
BF640890 similar to GP|10177523|dbj gene_id:K1F13.7~similar to u... 30 4.3
TC80505 29 7.4
AL369501 similar to GP|17976703|emb dihydrofolate synthetase /fo... 28 9.7
TC78893 similar to GP|16649013|gb|AAL24358.1 Unknown protein {Ar... 28 9.7
>TC81680 similar to GP|6729044|gb|AAF27040.1| hypothetical protein
{Arabidopsis thaliana}, partial (5%)
Length = 750
Score = 83.2 bits (204), Expect = 3e-16
Identities = 36/37 (97%), Positives = 37/37 (99%)
Frame = +2
Query: 662 QSWGRLPSSVRCWTMPEKLHSLCNPITENYFGGIT*Q 698
+SWGRLPSSVRCWTMPEKLHSLCNPITENYFGGIT*Q
Sbjct: 509 RSWGRLPSSVRCWTMPEKLHSLCNPITENYFGGIT*Q 619
Score = 70.9 bits (172), Expect = 2e-12
Identities = 41/70 (58%), Positives = 46/70 (65%), Gaps = 3/70 (4%)
Frame = +3
Query: 665 GRLPSSVRCWTMPEK---LHSLCNPITENYFGGIT*QAEADLLYSLITKAKFENNRYQKP 721
G+ P S + P + L L N I + + AEADLLYSLITKAKFENNRYQKP
Sbjct: 546 GQCPRSCIHYVTPSQRIILEELLNSIIDAPYDT---SAEADLLYSLITKAKFENNRYQKP 716
Query: 722 KKKTKFSTQH 731
KKKTKFSTQH
Sbjct: 717 KKKTKFSTQH 746
>BE248562
Length = 348
Score = 35.8 bits (81), Expect = 0.061
Identities = 14/14 (100%), Positives = 14/14 (100%)
Frame = -3
Query: 721 PKKKTKFSTQHVDW 734
PKKKTKFSTQHVDW
Sbjct: 346 PKKKTKFSTQHVDW 305
>AW257410 weakly similar to GP|10177533|db selenium-binding protein-like
{Arabidopsis thaliana}, partial (21%)
Length = 724
Score = 32.7 bits (73), Expect = 0.51
Identities = 10/16 (62%), Positives = 13/16 (80%)
Frame = +3
Query: 116 RYCIVEYNDFWVFKEW 131
RYC +EYND W++K W
Sbjct: 471 RYCFLEYND*WIYKMW 518
>TC81653 similar to SP|P93149|C9B1_GLYEC Cytochrome P450 93B1 (EC 1.14.-.-)
((2S)-flavanone 2-hydroxylase) (Licodione synthase),
partial (62%)
Length = 1193
Score = 32.7 bits (73), Expect = 0.51
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = -3
Query: 43 PRRFNISSYPLRQRPKPHSRFFHPCPNHQTTTFLR 77
P+ F++S+ P QR KPH + +P P FL+
Sbjct: 195 PQLFSLSNNPFHQRIKPHKQILYPLPKPLQIKFLK 91
>TC78402 weakly similar to PIR|B84853|B84853 hypothetical protein At2g42370
[imported] - Arabidopsis thaliana, partial (14%)
Length = 1982
Score = 31.2 bits (69), Expect = 1.5
Identities = 16/46 (34%), Positives = 20/46 (42%), Gaps = 7/46 (15%)
Frame = -1
Query: 30 PIPISFNHKISPQPRRF-------NISSYPLRQRPKPHSRFFHPCP 68
P P S +H P P R ++S +PL P PH HP P
Sbjct: 566 PHPFSHHHSHPPHPSRPPHLPLPPHLSPHPLPPHPSPHPLLLHPPP 429
>TC86734 similar to PIR|D86257|D86257 hypothetical protein [imported] -
Arabidopsis thaliana, partial (78%)
Length = 1429
Score = 30.0 bits (66), Expect = 3.3
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +1
Query: 528 WWCFSLASIAWCL*HTW*FRDG 549
W CF+ + A CL*HTW +++G
Sbjct: 511 WPCFN*SGCASCL*HTWHYQEG 576
>BF640890 similar to GP|10177523|dbj gene_id:K1F13.7~similar to unknown
protein~sp|P55606 {Arabidopsis thaliana}, partial (4%)
Length = 373
Score = 29.6 bits (65), Expect = 4.3
Identities = 23/63 (36%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = +1
Query: 17 FSKSTLQHKATTFPIPISFNHKISPQPRRFNISSYP-LRQRPKPHSRFFHPCPNHQTTTF 75
F + L+H+A P P+ F SP F S +P R RP+ R FH +++T TF
Sbjct: 34 FLRRNLRHQAQRTPFPLRFTSLQSPP---FLRSHFPQTRHRPR---RCFH--RSNRTKTF 189
Query: 76 LRF 78
RF
Sbjct: 190TRF 198
>TC80505
Length = 675
Score = 28.9 bits (63), Expect = 7.4
Identities = 16/38 (42%), Positives = 21/38 (55%)
Frame = +1
Query: 41 PQPRRFNISSYPLRQRPKPHSRFFHPCPNHQTTTFLRF 78
P P ++ S P R + HS FF P +H TTTF+ F
Sbjct: 43 PLPLFSSLLSTPFRSHLQHHS-FFIPNYHHNTTTFISF 153
>AL369501 similar to GP|17976703|emb dihydrofolate synthetase
/folylpolyglutamate synthetase {Arabidopsis thaliana},
partial (10%)
Length = 470
Score = 28.5 bits (62), Expect = 9.7
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +2
Query: 30 PIPISFNHKISPQPRRFNISSYPLRQRPKPHSRFFH 65
P PI F +SP P+ N+ R+ PH+++FH
Sbjct: 11 PTPIPFPATLSPAPQ-LNLPPEKSRRAVPPHNQYFH 115
>TC78893 similar to GP|16649013|gb|AAL24358.1 Unknown protein {Arabidopsis
thaliana}, partial (32%)
Length = 805
Score = 28.5 bits (62), Expect = 9.7
Identities = 17/44 (38%), Positives = 22/44 (49%), Gaps = 4/44 (9%)
Frame = +3
Query: 58 KPHSRFFHPCPNHQ----TTTFLRF*WLPTECPFYLELSAFNVF 97
+PH +F H +H T TFL F +LPT P L + F F
Sbjct: 15 QPHCQFIHHRSSHSLPLTTITFLSFLYLPTNQPQTLFHNIFYFF 146
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.365 0.163 0.692
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,970,806
Number of Sequences: 36976
Number of extensions: 564888
Number of successful extensions: 8845
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 2010
Number of HSP's successfully gapped in prelim test: 411
Number of HSP's that attempted gapping in prelim test: 6628
Number of HSP's gapped (non-prelim): 2850
length of query: 734
length of database: 9,014,727
effective HSP length: 103
effective length of query: 631
effective length of database: 5,206,199
effective search space: 3285111569
effective search space used: 3285111569
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC135319.9