BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135315.2 - phase: 0
(478 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CA921522 245 2e-65
BE203370 156 2e-38
BQ123351 102 4e-22
AL366606 101 6e-22
AW736370 44 2e-04
TC77655 similar to PIR|T09217|T09217 protein sam2B - spinach, pa... 30 2.0
TC77925 similar to GP|18700163|gb|AAL77693.1 AT4g02720/T10P11_1 ... 30 2.0
CA918173 weakly similar to GP|11034537|dbj putative glucosyl tra... 30 2.7
TC80041 similar to GP|8778702|gb|AAF79710.1| T1N15.20 {Arabidops... 29 3.5
TC89517 weakly similar to PIR|A85475|A85475 hypothetical protein... 29 4.5
TC82133 similar to PIR|G84597|G84597 probable XAP-5 protein [Hom... 28 7.7
>CA921522
Length = 767
Score = 245 bits (626), Expect = 2e-65
Identities = 124/252 (49%), Positives = 164/252 (64%), Gaps = 4/252 (1%)
Frame = +3
Query: 111 KTSDVSKHLITKSHIRGFTSKYLLVQANL--ETTCQDALEAILALLIYGLILFPNLDNFV 168
+T +V HLIT+ GF++ +L + + A +ILALL+YGL+LFP+LDNFV
Sbjct: 3 ETDEVKTHLITRGKFLGFSTDFLYERTTFFDKMGVAYAFNSILALLVYGLVLFPSLDNFV 182
Query: 169 DMNAIEIFHSRNPVPTLLANMYHAIHDRTLKGRGYILCCVPLLYRWFISHLPSS--FHDN 226
D+ AI+IF SRNPVPTLL + Y +IH RT GRG ILCC LLYRW SHLP + F N
Sbjct: 183 DIKAIQIFLSRNPVPTLLGDTYLSIHRRTQAGRGTILCCAQLLYRWITSHLPRTPRFTTN 362
Query: 227 SEDWSYSQRMMALSPNEVVWITPAAQVKEIITGCGDFLNVPLLGTRGGINYNPELAMRQF 286
E+ +S+R+M+L+P EVVW II CG F NVPLLG GGI+YNP LA QF
Sbjct: 363 PENLLWSKRLMSLTPAEVVWYDRVYDKGTIIDSCGKFANVPLLGMEGGISYNPTLARHQF 542
Query: 287 GFPMKTKPINLATSPEFFYYSNAPTGQREAFTRAWSKVRRKSVRHLGVRSGIAHEAYTQW 346
G+PM+ KP+++ ++ ++ TG RE RAW +RR+ LG ++G +YTQW
Sbjct: 543 GYPMERKPLSIYLENVYYLNADDSTGMREHVVRAWHTIRRRDKDQLGKKTGAISSSYTQW 722
Query: 347 VINRAEEIGMPY 358
VI+RA +IGMPY
Sbjct: 723 VIDRAVQIGMPY 758
>BE203370
Length = 454
Score = 156 bits (394), Expect = 2e-38
Identities = 80/150 (53%), Positives = 105/150 (69%), Gaps = 2/150 (1%)
Frame = +1
Query: 63 CFTFPDFQLVPTLEAYSYLLGSPIAEKTPFTGPGASLTPLVIAKDLYLKTSDVSKHLITK 122
CFTFPD+QL PTLE YSYL+G P+ +K PFTG IA L+L+TS + L K
Sbjct: 4 CFTFPDYQLAPTLEEYSYLVG*PVLDKVPFTGFEPIPKAATIADALHLETSLIKAKLTIK 183
Query: 123 SHIRGFTSKYLLVQAN--LETTCQDALEAILALLIYGLILFPNLDNFVDMNAIEIFHSRN 180
++ +K+L QA+ + DA +ILALLIYGL+LFPN+DN+VD++AI++F ++N
Sbjct: 184 GNLPSLPTKFLYQQASDFAKVDNVDAFYSILALLIYGLVLFPNVDNYVDIHAIQMFLTKN 363
Query: 181 PVPTLLANMYHAIHDRTLKGRGYILCCVPL 210
PVPTLLA+MYH+IHDR GRG IL C PL
Sbjct: 364 PVPTLLADMYHSIHDRNQVGRGAILGCAPL 453
>BQ123351
Length = 725
Score = 102 bits (253), Expect = 4e-22
Identities = 58/157 (36%), Positives = 94/157 (58%)
Frame = +2
Query: 317 FTRAWSKVRRKSVRHLGVRSGIAHEAYTQWVINRAEEIGMPYPAMRHVAASAPSIPLPLP 376
F +AW V ++ LG RS + +E+YT+WVI+RA + G+P+P R +++ S LP+
Sbjct: 8 FIQAWRAVHKREKGQLGRRS*LVYESYTKWVIDRAAKNGIPFPLQRLPSSTTLSPSLPMT 187
Query: 377 PTTQEMYQEHLAMESRKKQMWKARYNEAENLIMTLDGKDEQKTHENLMLKKELVKVRREL 436
T+E Q LA +R+K W+ RY EAE+ I TL G+ EQK HE L +++++++ L
Sbjct: 188 SKTKEEAQYLLAEMTREKDTWRIRYMEAESEIGTLRGQVEQKDHELLKMRQQMIERDDLL 367
Query: 437 EEKDELLMRDSKRARGRRNFYARYCGSDSESESEDHP 473
+EKD+LL + + + R + + G DS+ E P
Sbjct: 368 QEKDKLLKKHITK-KQRMDSMDLFDGPDSDFED*SFP 475
>AL366606
Length = 393
Score = 101 bits (252), Expect = 6e-22
Identities = 55/81 (67%), Positives = 59/81 (71%), Gaps = 6/81 (7%)
Frame = -1
Query: 146 ALEAILALLIYGLILFPNLDNFVDMNAIEIFHSRNPVPTLLANMYHAIHDRTLKGRGYIL 205
A AI L GLILFPNLDNFVDMNAIE+FHS+NPVPTLLA+ YHAIHDRTLKGRGYIL
Sbjct: 390 APSAIXLCLSTGLILFPNLDNFVDMNAIEVFHSKNPVPTLLADTYHAIHDRTLKGRGYIL 211
Query: 206 CCVPLL------YRWFISHLP 220
L +R F S LP
Sbjct: 210 WLHILFSNRVVYFRTFRSSLP 148
>AW736370
Length = 488
Score = 43.5 bits (101), Expect = 2e-04
Identities = 25/92 (27%), Positives = 44/92 (47%)
Frame = -2
Query: 366 ASAPSIPLPLPPTTQEMYQEHLAMESRKKQMWKARYNEAENLIMTLDGKDEQKTHENLML 425
A P+ PLP+ T++ YQE L R+ WK Y + + T+ G EQ+ +++
Sbjct: 487 AITPAPPLPMTFDTEKEYQERLTEAEREIHRWKREYQKKDQDYETVMGLLEQEAYDSR*K 308
Query: 426 KKELVKVRRELEEKDELLMRDSKRARGRRNFY 457
+ K+ ++E D L R R + R + +
Sbjct: 307 DVIIAKLNERIKENDAALDRIPGRKKRRMDLF 212
>TC77655 similar to PIR|T09217|T09217 protein sam2B - spinach, partial (64%)
Length = 1341
Score = 30.0 bits (66), Expect = 2.0
Identities = 16/48 (33%), Positives = 29/48 (60%), Gaps = 3/48 (6%)
Frame = +3
Query: 412 DGKDEQKTHENLMLKKELVKVRRELEEK---DELLMRDSKRARGRRNF 456
DG++E + + + +KKEL KV +E E+ +L+ K+A GR ++
Sbjct: 468 DGEEESEEEKRMRVKKELEKVAKEQAERRATAQLMYDLGKKAYGRGSY 611
>TC77925 similar to GP|18700163|gb|AAL77693.1 AT4g02720/T10P11_1
{Arabidopsis thaliana}, partial (47%)
Length = 1538
Score = 30.0 bits (66), Expect = 2.0
Identities = 17/44 (38%), Positives = 24/44 (53%)
Frame = +3
Query: 427 KELVKVRRELEEKDELLMRDSKRARGRRNFYARYCGSDSESESE 470
KE+ + + E +E DS+ + RR Y + SDSESESE
Sbjct: 522 KEIKQKAKSESESEESKESDSELRKKRRKSYKKSRESDSESESE 653
>CA918173 weakly similar to GP|11034537|dbj putative glucosyl transferase
{Oryza sativa (japonica cultivar-group)}, partial (19%)
Length = 769
Score = 29.6 bits (65), Expect = 2.7
Identities = 13/22 (59%), Positives = 18/22 (81%)
Frame = -3
Query: 49 LVHTLVQFYDPSFRCFTFPDFQ 70
L+HTLVQ + PS RCF FP+++
Sbjct: 113 LIHTLVQKW-PS*RCFPFPNYE 51
>TC80041 similar to GP|8778702|gb|AAF79710.1| T1N15.20 {Arabidopsis
thaliana}, partial (7%)
Length = 1621
Score = 29.3 bits (64), Expect = 3.5
Identities = 31/116 (26%), Positives = 46/116 (38%), Gaps = 11/116 (9%)
Frame = +3
Query: 369 PSIPLPLPPTTQEMYQEHLAMESRKKQMWKARYNEAENLIMT-----LDGKDEQKT---H 420
PS L LP + +L +ESR +++ ENL+++ G QKT
Sbjct: 705 PSSVLSLPQERTQGILNNLRVESRFREVAGNGKQRDENLVLSGCHFDNGGDQNQKTVSDR 884
Query: 421 ENLMLKKELVKVRRELEEKDELLMRDSKRARGRRNFYARYCGSDSESESED---HP 473
ENL L++++V+ + CG D ESE ED HP
Sbjct: 885 ENLSLRQDIVRPVEAI------------------------CGGDLESEMEDQQNHP 980
>TC89517 weakly similar to PIR|A85475|A85475 hypothetical protein AT4g40080
[imported] - Arabidopsis thaliana, partial (46%)
Length = 1177
Score = 28.9 bits (63), Expect = 4.5
Identities = 23/87 (26%), Positives = 35/87 (39%), Gaps = 1/87 (1%)
Frame = -3
Query: 161 FPNLDNFVDMNAIEIFHSRNPVPTLLANMYHAIHDRTLKGRGYILCCVPLLYRWFISHLP 220
F L F++ N H NP+ ++H IH Y+ + L W I P
Sbjct: 764 FLPLPKFINSNQ-NFIHHHNPI------IFHQIH--------YLNNHLVLFTHWSIRFFP 630
Query: 221 SSFHDNSEDWSYS-QRMMALSPNEVVW 246
+SFH + S+S Q + N +W
Sbjct: 629 NSFHQGQQRISFS*QITIGKIRNSFLW 549
>TC82133 similar to PIR|G84597|G84597 probable XAP-5 protein [Homo sapiens]
[imported] - Arabidopsis thaliana, partial (60%)
Length = 926
Score = 28.1 bits (61), Expect = 7.7
Identities = 17/84 (20%), Positives = 37/84 (43%), Gaps = 3/84 (3%)
Frame = -2
Query: 303 FFYYSNAPTGQREAFTRAWSKVRR---KSVRHLGVRSGIAHEAYTQWVINRAEEIGMPYP 359
F YS+ T +F +A S++ + R G +A ++ W+ R+ +
Sbjct: 400 FSTYSSRVTSPTVSFLKAVSRISEVLEPNWRRPGWPLEVADLLFSSWIFRRSASLCFSSR 221
Query: 360 AMRHVAASAPSIPLPLPPTTQEMY 383
+R + + P+ P P+P + ++
Sbjct: 220 RIRTASWAVPTYPSPIPDISLALF 149
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.136 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,842,255
Number of Sequences: 36976
Number of extensions: 211430
Number of successful extensions: 1241
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1221
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1237
length of query: 478
length of database: 9,014,727
effective HSP length: 100
effective length of query: 378
effective length of database: 5,317,127
effective search space: 2009874006
effective search space used: 2009874006
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC135315.2