BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135311.2 + phase: 0
(164 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC88968 similar to GP|12329988|emb|CAC24566. trypsin inhibitor {... 252 4e-68
TC86596 similar to PIR|S56648|S56648 trypsin inhibitor precursor... 155 9e-39
TC78590 similar to PIR|S56647|S56647 trypsin inhibitor precursor... 137 2e-33
TC86991 weakly similar to PIR|S56648|S56648 trypsin inhibitor pr... 126 3e-30
TC81432 similar to PIR|S56647|S56647 trypsin inhibitor precursor... 119 7e-28
BE998577 weakly similar to GP|17059630|emb Trypsin/chymotrypsin ... 91 2e-19
TC92647 similar to GP|17059630|emb|CAD12253. Trypsin/chymotrypsi... 55 1e-13
AW736473 similar to PIR|C84201|C842 multidrug resistance protein... 33 0.067
TC78692 similar to GP|13384384|gb|AAK21352.1 putative extensin {... 32 0.15
TC76916 MtN4 30 0.43
AI974295 similar to GP|9759211|db gene_id:MXC17.10~unknown prote... 29 0.74
TC87435 similar to GP|3549652|emb|CAA12272.1 MAP3K epsilon prote... 29 0.97
BQ137224 similar to GP|1934808|emb| unknown {Bacillus subtilis},... 29 0.97
AW586908 weakly similar to GP|2104814|emb| pleiotropic effects o... 28 1.3
BF644693 homologue to GP|2598599|emb MtN4 {Medicago truncatula},... 28 1.3
TC88336 similar to PIR|T51477|T51477 glutamine-rich protein - Ar... 28 1.7
BG645829 similar to SP|O22307|C7DB Cytochrome P450 71D11 (EC 1.1... 28 1.7
CA859527 28 1.7
TC85814 similar to SP|P48631|FD62_SOYBN Omega-6 fatty acid desat... 28 2.2
BM814918 homologue to SP|P48631|FD62_ Omega-6 fatty acid desatur... 28 2.2
>TC88968 similar to GP|12329988|emb|CAC24566. trypsin inhibitor {Pisum
sativum}, partial (73%)
Length = 546
Score = 252 bits (644), Expect = 4e-68
Identities = 116/116 (100%), Positives = 116/116 (100%)
Frame = +1
Query: 1 MELMNKNKKAMVMKLALLVFLLGFTSTVVDARFDSTSFITQVISNGDSTTNNYDAKSTAT 60
MELMNKNKKAMVMKLALLVFLLGFTSTVVDARFDSTSFITQVISNGDSTTNNYDAKSTAT
Sbjct: 61 MELMNKNKKAMVMKLALLVFLLGFTSTVVDARFDSTSFITQVISNGDSTTNNYDAKSTAT 240
Query: 61 ACCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSSPLCTCYDITDFCYKPCN 116
ACCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSSPLCTCYDITDFCYKPCN
Sbjct: 241 ACCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSSPLCTCYDITDFCYKPCN 408
>TC86596 similar to PIR|S56648|S56648 trypsin inhibitor precursor (clone
ATI21) - alfalfa, partial (95%)
Length = 590
Score = 155 bits (391), Expect = 9e-39
Identities = 79/119 (66%), Positives = 92/119 (76%), Gaps = 3/119 (2%)
Frame = +3
Query: 1 MELMNKNKKAMVMKLALLVFLLGFTSTVVDARFDSTSFITQVISNGDSTTNNYDAKSTAT 60
M+LM NKKAMVMKLALLVFLLGFTSTVVDARFD +SFITQ++SNG++T Y+ KST T
Sbjct: 57 MDLM-MNKKAMVMKLALLVFLLGFTSTVVDARFDGSSFITQLLSNGEAT---YEVKSTTT 224
Query: 61 ACCNTCLC--SVTPELTQCRCADVGKTCHSACKNCECGWS-SPLCTCYDITDFCYKPCN 116
ACCN+C C S+ P QC CAD+G+ CHSACK C C S P C C D TDFCY+PC+
Sbjct: 225 ACCNSCPCTKSIPP---QCHCADIGEKCHSACKRCLCTRSFPPQCRCTDTTDFCYEPCS 392
>TC78590 similar to PIR|S56647|S56647 trypsin inhibitor precursor (clone
ATI18) - alfalfa, partial (94%)
Length = 649
Score = 137 bits (345), Expect = 2e-33
Identities = 70/113 (61%), Positives = 83/113 (72%), Gaps = 3/113 (2%)
Frame = +1
Query: 7 NKKAMVMKLALLVFLLGFTSTVVDARFDSTSFITQVISNGDSTTNNYDAKSTATACCNTC 66
NKKAM+MKLALLVFLL FTST+VDARFD SFITQ++SNG++ T KST TACC+ C
Sbjct: 184 NKKAMMMKLALLVFLLSFTSTMVDARFDRASFITQLLSNGEANT-----KSTTTACCDFC 348
Query: 67 LC--SVTPELTQCRCADVGKTCHSACKNCECGWS-SPLCTCYDITDFCYKPCN 116
C S+ P QC+C DV + CHSACK+C C S P C CYDIT+FCY C+
Sbjct: 349 PCTRSIPP---QCQCTDVKEKCHSACKSCLCTRSFPPQCRCYDITNFCYPSCS 498
Score = 34.3 bits (77), Expect = 0.023
Identities = 20/40 (50%), Positives = 24/40 (60%)
Frame = +2
Query: 125 QVVVTIVLAQDHSHLIVLVMMLNHFVTQIASLAGASSHYL 164
Q V LAQD SHL V V+ML VTQ+A +A A +L
Sbjct: 329 QHAVISALAQDQSHLNVSVLMLKKNVTQLAKVAFAHGLFL 448
>TC86991 weakly similar to PIR|S56648|S56648 trypsin inhibitor precursor
(clone ATI21) - alfalfa, partial (54%)
Length = 587
Score = 126 bits (317), Expect = 3e-30
Identities = 64/115 (55%), Positives = 74/115 (63%), Gaps = 3/115 (2%)
Frame = +2
Query: 5 NKNKKAMVMKLALLVFLLGFTSTVVDARFDSTSFITQVISNGDSTTNNYDAKSTATACCN 64
N + + MK+ALL+F LG T+T++DARF+ S ITQVISNG KST CCN
Sbjct: 20 NIREMGLKMKIALLLFFLGLTTTIIDARFEPNSLITQVISNG--------VKSTCKPCCN 175
Query: 65 TCLC--SVTPELTQCRCADVGKTCHSACKNCECGWSSPL-CTCYDITDFCYKPCN 116
C C S+ P QCRC DV TCHSACK C C S PL C C DITDFCY+PCN
Sbjct: 176 NCSCTFSIPP---QCRCKDVKPTCHSACKTCRCFESFPLKCDCLDITDFCYEPCN 331
Score = 42.0 bits (97), Expect = 1e-04
Identities = 23/43 (53%), Positives = 28/43 (64%)
Frame = +3
Query: 122 QKVQVVVTIVLAQDHSHLIVLVMMLNHFVTQIASLAGASSHYL 164
Q V +VV IV+A S L V+V MLN VTQ+A L G SH+L
Sbjct: 153 QHVNLVVIIVVAHFQSLLSVVVKMLNQHVTQLARLVGVLSHFL 281
>TC81432 similar to PIR|S56647|S56647 trypsin inhibitor precursor (clone
ATI18) - alfalfa, partial (83%)
Length = 569
Score = 119 bits (297), Expect = 7e-28
Identities = 63/105 (60%), Positives = 76/105 (72%), Gaps = 3/105 (2%)
Frame = +1
Query: 12 VMKLALLVFLLGFTSTVVDARFDSTSFITQVISNGDSTTNNYDAKSTATACCNTCLCSVT 71
+MKLALLVFLLGFTSTVVDARFD TSFITQVISNG++ YD KST T CCN+C C+ +
Sbjct: 88 MMKLALLVFLLGFTSTVVDARFDPTSFITQVISNGEAA---YDVKSTTTGCCNSCQCTRS 258
Query: 72 -PELTQCRCADVGKTCHS-ACKNCECGWS-SPLCTCYDITDFCYK 113
P+ C C DV + C+S C +C C S P C C+D+T FCY+
Sbjct: 259 FPQ--ACVCTDVREKCNSPGCLDCVCTKSIPPQCRCHDVTTFCYQ 387
Score = 29.6 bits (65), Expect = 0.57
Identities = 20/41 (48%), Positives = 24/41 (57%), Gaps = 1/41 (2%)
Frame = +2
Query: 125 QVVVTIVLAQDHSHLIVLVMMLNHFVT-QIASLAGASSHYL 164
Q V AQDHSH V V+ML VT Q+A +A A S +L
Sbjct: 224 QDAVIAANAQDHSHKHVCVLMLEKNVTHQVA*IAFAQSLFL 346
>BE998577 weakly similar to GP|17059630|emb Trypsin/chymotrypsin inhibitor
{Lens culinaris}, partial (33%)
Length = 551
Score = 91.3 bits (225), Expect = 2e-19
Identities = 56/121 (46%), Positives = 70/121 (57%), Gaps = 7/121 (5%)
Frame = +2
Query: 2 ELMNKNKKAMVMKLALLVFLLGFTSTVVDARFDSTSFITQVISNGDSTTNNYDAKSTATA 61
++M NK V KL++L+ LL FT+T+VDARFDST F T+V+SNG+ T YD +A
Sbjct: 5 KVMESNKNTFV-KLSVLLLLLCFTATIVDARFDSTPFTTRVLSNGEDT---YDV-IYHSA 169
Query: 62 CCNTCLCSVTPE--LTQCRCADVGKTCHSACKNCECGWSS----PLCTCYDITDF-CYKP 114
CC+ C C V QC C D G CHS CK C+C S P C C D+ F CY
Sbjct: 170 CCDKCECYVKESHFFPQCICRDDG--CHSPCKKCDCIRDSESETPSCYCADVMFFTCYDL 343
Query: 115 C 115
C
Sbjct: 344 C 346
>TC92647 similar to GP|17059630|emb|CAD12253. Trypsin/chymotrypsin
inhibitor {Lens culinaris}, partial (14%)
Length = 582
Score = 54.7 bits (130), Expect(2) = 1e-13
Identities = 32/47 (68%), Positives = 38/47 (80%), Gaps = 2/47 (4%)
Frame = +1
Query: 7 NKKAMVMKLALLVFLLGFTSTVVDARFDSTSFITQVISNGD--STTN 51
NKK M+ KLA+L+FLL F +T+ DARFDS SFITQVIS+ D STTN
Sbjct: 43 NKKTMI-KLAMLIFLLSFIATI-DARFDSKSFITQVISSNDVNSTTN 177
Score = 37.0 bits (84), Expect(2) = 1e-13
Identities = 15/35 (42%), Positives = 18/35 (50%)
Frame = +2
Query: 77 CRCADVGKTCHSACKNCECGWSSPLCTCYDITDFC 111
C+C D+ +TC S CK C C CTC D C
Sbjct: 230 CQCLDIVETCESTCKYCTCK-QPKRCTCDDYEQDC 331
>AW736473 similar to PIR|C84201|C842 multidrug resistance protein homolog
[imported] - Halobacterium sp. NRC-1, partial (3%)
Length = 443
Score = 32.7 bits (73), Expect = 0.067
Identities = 14/52 (26%), Positives = 23/52 (43%), Gaps = 1/52 (1%)
Frame = -1
Query: 68 CSVTPEL-TQCRCADVGKTCHSACKNCECGWSSPLCTCYDITDFCYKPCNVW 118
CS+ + TQC+ + C++ C +C W+ C D CY +W
Sbjct: 212 CSLLDQCYTQCQSYSLLDRCYAQCPSCSLDWNCSQCPSCSRLDPCYAQWPLW 57
>TC78692 similar to GP|13384384|gb|AAK21352.1 putative extensin {Oryza
sativa (japonica cultivar-group)}, partial (21%)
Length = 1264
Score = 31.6 bits (70), Expect = 0.15
Identities = 18/59 (30%), Positives = 23/59 (38%), Gaps = 2/59 (3%)
Frame = +2
Query: 48 STTNNYDAKSTATAC--CNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSSPLCTC 104
ST T + C CNTC+ T T +D S+C NC S C+C
Sbjct: 488 STNETLPWDCTCSVC*DCNTCVLCTTSSTTYLSASD---NTGSSCTNCSSSQYSASCSC 655
>TC76916 MtN4
Length = 1850
Score = 30.0 bits (66), Expect = 0.43
Identities = 22/75 (29%), Positives = 33/75 (43%), Gaps = 2/75 (2%)
Frame = +3
Query: 36 TSFITQVISNGDSTTNNYDAKSTATACCNTC--LCSVTPELTQCRCADVGKTCHSACKNC 93
T ++ I N +T+++ ST T C T LCS T T C ++ T + +C
Sbjct: 633 TITLSTPIINTKTTSSSQTPSSTPTTCSKTTSTLCSKT---TNC*TSNSTSTLRTKTPSC 803
Query: 94 ECGWSSPLCTCYDIT 108
E P TC + T
Sbjct: 804 ETTTICP*TTCCETT 848
Score = 28.5 bits (62), Expect = 1.3
Identities = 20/57 (35%), Positives = 21/57 (36%)
Frame = +3
Query: 60 TACCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSSPLCTCYDITDFCYKPCN 116
T CC T LCS T T C C T S NC S +C T C N
Sbjct: 828 TTCCETTLCS*T---TSCTC---NTTLCS*ATNCFPTTCSSTSSCTITTTLCTNTSN 980
>AI974295 similar to GP|9759211|db gene_id:MXC17.10~unknown protein
{Arabidopsis thaliana}, partial (14%)
Length = 478
Score = 29.3 bits (64), Expect = 0.74
Identities = 14/45 (31%), Positives = 18/45 (39%), Gaps = 1/45 (2%)
Frame = +3
Query: 76 QCRCADVGKTCHSACKNCECGWSSPLC-TCYDITDFCYKPCNVWQ 119
+CRC G C + + C W S C T I PC W+
Sbjct: 84 RCRCYSTG*NCPRSSEEVSCSWIS*RCFTRS*IEGISIAPCQSWR 218
>TC87435 similar to GP|3549652|emb|CAA12272.1 MAP3K epsilon protein kinase
{Arabidopsis thaliana}, partial (29%)
Length = 1646
Score = 28.9 bits (63), Expect = 0.97
Identities = 18/63 (28%), Positives = 27/63 (42%), Gaps = 7/63 (11%)
Frame = +2
Query: 57 STATACCNTCLCSVTPELTQCR-CADVGKTCHSACKNCE------CGWSSPLCTCYDITD 109
STA C++ C V PEL++ C +G + K C C W + + C +
Sbjct: 971 STAEEGCSSEACDVLPELSRAAFCTHIGAILENHHKICPDQYHFGC*WINSITRCKA*SS 1150
Query: 110 FCY 112
CY
Sbjct: 1151 RCY 1159
>BQ137224 similar to GP|1934808|emb| unknown {Bacillus subtilis}, partial
(17%)
Length = 1046
Score = 28.9 bits (63), Expect = 0.97
Identities = 18/61 (29%), Positives = 28/61 (45%)
Frame = -1
Query: 58 TATACCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSSPLCTCYDITDFCYKPCNV 117
+ATA CLC+ Q C V ++C A + CG + +C D+ D Y C +
Sbjct: 995 SATARTRVCLCA------QSVCVSV*RSCIYASRLVACGSLACYVSCRDV-DVIYLHCRL 837
Query: 118 W 118
+
Sbjct: 836 Y 834
>AW586908 weakly similar to GP|2104814|emb| pleiotropic effects on cellular
differentiation and slug behaviour {Dictyostelium
discoideum}, partial (8%)
Length = 668
Score = 28.5 bits (62), Expect = 1.3
Identities = 19/60 (31%), Positives = 22/60 (36%), Gaps = 2/60 (3%)
Frame = -3
Query: 60 TACCNTCLCSVTPELTQCRCADVGKTCHSACKNCE-CGWSSPLC-TCYDITDFCYKPCNV 117
+ CC C C C C + C NC C WS C T DI C CN+
Sbjct: 636 SGCCCCCCC--------CCCCNF-------CNNCNCCCWSEMFCGTV*DICGCCGPLCNM 502
>BF644693 homologue to GP|2598599|emb MtN4 {Medicago truncatula}, partial
(79%)
Length = 621
Score = 28.5 bits (62), Expect = 1.3
Identities = 20/57 (35%), Positives = 21/57 (36%)
Frame = +1
Query: 60 TACCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSSPLCTCYDITDFCYKPCN 116
T CC T LCS T T C C T S NC S +C T C N
Sbjct: 55 TTCCETTLCS*T---TSCTC---NTTLCS*ATNCFPTTCSSTSSCTITTTLCTNTSN 207
Score = 26.9 bits (58), Expect = 3.7
Identities = 18/60 (30%), Positives = 23/60 (38%), Gaps = 11/60 (18%)
Frame = +1
Query: 49 TTNNYDA--KSTATACCNTCLCSVTP---ELTQCRCADVGKT------CHSACKNCECGW 97
TTN + ST++ CNT LC+ T T C + T C C C W
Sbjct: 214 TTNCFSTTCSSTSSCACNTTLCATTTYCNSTTTCTLSTTTSTTNMFH*CFEVGCMC*CAW 393
>TC88336 similar to PIR|T51477|T51477 glutamine-rich protein - Arabidopsis
thaliana, partial (67%)
Length = 1297
Score = 28.1 bits (61), Expect = 1.7
Identities = 15/49 (30%), Positives = 17/49 (34%)
Frame = -2
Query: 56 KSTATACCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSSPLCTC 104
+ A ACC C C C C K C C+ C S C C
Sbjct: 339 REIAAACCCCCCCF-------CNCCKSIKCCCCCCRCCS*INSCCCCCC 214
Score = 25.8 bits (55), Expect = 8.2
Identities = 12/43 (27%), Positives = 16/43 (36%), Gaps = 4/43 (9%)
Frame = -2
Query: 57 STATACCNTCLCSVTPEL----TQCRCADVGKTCHSACKNCEC 95
+ A CC C C+ + CRC +C C C C
Sbjct: 330 AAACCCCCCCFCNCCKSIKCCCCCCRCCS*INSCCCCCCCCCC 202
>BG645829 similar to SP|O22307|C7DB Cytochrome P450 71D11 (EC 1.14.-.-)
(Fragment). {Lotus japonicus}, partial (41%)
Length = 757
Score = 28.1 bits (61), Expect = 1.7
Identities = 17/56 (30%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
Frame = +2
Query: 105 YDITDFCYKPC-NVWQQQQKVQVVVTIVLAQDHSHLIVLVMMLNHFVTQIASLAGA 159
YD TD + P + W+Q +K+ V + L + S + +N+ V +IAS G+
Sbjct: 308 YDSTDIAFAPFGDYWRQLRKICTVELLSLKRVQSLSPIREQEMNNLVKRIASEEGS 475
>CA859527
Length = 501
Score = 28.1 bits (61), Expect = 1.7
Identities = 17/52 (32%), Positives = 22/52 (41%)
Frame = +3
Query: 62 CCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSSPLCTCYDITDFCYK 113
C ++C CS + L V K + K CEC S C C D + C K
Sbjct: 138 CSSSCNCSNSKVL-------VNKASETNPKECECTSSQGGCVCGDGCECC*K 272
>TC85814 similar to SP|P48631|FD62_SOYBN Omega-6 fatty acid desaturase
endoplasmic reticulum isozyme 2 (EC 1.14.99.-).
[Soybean], complete
Length = 1897
Score = 27.7 bits (60), Expect = 2.2
Identities = 19/58 (32%), Positives = 22/58 (37%), Gaps = 4/58 (6%)
Frame = +1
Query: 62 CCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSSPLCTCY----DITDFCYKPC 115
C NTC SV L C +C C C WSS C+ + D Y PC
Sbjct: 1012 CRNTC--SVLRALPSCHG-------QRSCLGCVCLWSSTACSQWILGIDYIFAAYSPC 1158
>BM814918 homologue to SP|P48631|FD62_ Omega-6 fatty acid desaturase
endoplasmic reticulum isozyme 2 (EC 1.14.99.-).
[Soybean], partial (18%)
Length = 672
Score = 27.7 bits (60), Expect = 2.2
Identities = 19/58 (32%), Positives = 22/58 (37%), Gaps = 4/58 (6%)
Frame = +1
Query: 62 CCNTCLCSVTPELTQCRCADVGKTCHSACKNCECGWSSPLCTCY----DITDFCYKPC 115
C NTC SV L C +C C C WSS C+ + D Y PC
Sbjct: 493 CRNTC--SVLRALPSCHG-------QRSCLGCVCLWSSTACSQWILGIDYIFAAYSPC 639
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.131 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,867,651
Number of Sequences: 36976
Number of extensions: 141466
Number of successful extensions: 1414
Number of sequences better than 10.0: 80
Number of HSP's better than 10.0 without gapping: 1357
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1389
length of query: 164
length of database: 9,014,727
effective HSP length: 89
effective length of query: 75
effective length of database: 5,723,863
effective search space: 429289725
effective search space used: 429289725
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 54 (25.4 bits)
Medicago: description of AC135311.2