Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC135234.5 - phase: 0 /pseudo
         (256 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC88749                                                                47  7e-06
BG588495                                                               29  2.0
AW689585 homologue to GP|21166122|gb|A hypothetical protein {Dic...    28  4.5
BF645909 weakly similar to GP|9758286|dbj cytochrome P450-like p...    27  5.9
TC81007 similar to GP|17473902|gb|AAL38369.1 RNA helicase - like...    27  5.9
TC87681 similar to GP|17381090|gb|AAL36357.1 unknown protein {Ar...    27  7.7
TC83355 similar to GP|6016726|gb|AAF01552.1| unknown protein {Ar...    27  7.7

>TC88749 
          Length = 1221

 Score = 47.0 bits (110), Expect = 7e-06
 Identities = 22/52 (42%), Positives = 34/52 (65%)
 Frame = +2

Query: 76  WREREELLTWVRRQGVKAGFGVCIDKSVLKRPYLTTQSERSGIYKPPKTRKK 127
           W+++E++L+ +RRQ  +AGF V I +S ++ P L    ERSG +K  K R K
Sbjct: 551 WKDKEDMLSSIRRQANRAGFTVGIKRSSIQNPMLELMCERSGDHKVSKKRLK 706


>BG588495 
          Length = 540

 Score = 28.9 bits (63), Expect = 2.0
 Identities = 11/21 (52%), Positives = 15/21 (71%)
 Frame = +3

Query: 77 REREELLTWVRRQGVKAGFGV 97
          ++R+ELL W R Q  KAGF +
Sbjct: 21 KDRDELLEWTRHQANKAGFTI 83


>AW689585 homologue to GP|21166122|gb|A hypothetical protein {Dictyostelium
           discoideum}, partial (13%)
          Length = 465

 Score = 27.7 bits (60), Expect = 4.5
 Identities = 21/64 (32%), Positives = 31/64 (47%)
 Frame = +1

Query: 153 NDWWLAILCGMHNHDLEEKLQGHLIAGRLSAEEKKKVIDMTKSLTVPRNILTNLKENNKE 212
           N W L IL  +H+H L+  LQ    A  +  E K  ++++T S  V    + NL    K 
Sbjct: 25  NQWLLTILSLLHHHLLQSLLQNQ--ACFIMVEVKISMMNLTSSKLV--IFVENLLAKTKT 192

Query: 213 SVTT 216
           S+ T
Sbjct: 193 SLCT 204


>BF645909 weakly similar to GP|9758286|dbj cytochrome P450-like protein
           {Arabidopsis thaliana}, partial (27%)
          Length = 648

 Score = 27.3 bits (59), Expect = 5.9
 Identities = 30/132 (22%), Positives = 53/132 (39%), Gaps = 11/132 (8%)
 Frame = +2

Query: 76  WREREELLTWVRRQGVKAGFGVCIDKSVLKRPYLTTQSERSGIYKPPKTRKKPNLEGIGS 135
           ++ R  LL W  +   ++     + + +  R  + T ++ +  Y   KT  K   +G   
Sbjct: 230 YKNRFRLLDWYTKLLAQSPTNTILVQRLGARRTIVTANQHNVEYVL-KTNFKNFPKGKPF 406

Query: 136 MKCNCPFRLKGFFDKDTNDWWLAILCGMHN-----------HDLEEKLQGHLIAGRLSAE 184
            +    F  KG F+ D + W +      H            H LEE+++G LI    S  
Sbjct: 407 REILSDFLGKGIFNVDDDLWMIQRKLASHEFSLRSINEFIMHTLEEEVKGKLIPLMDSLS 586

Query: 185 EKKKVIDMTKSL 196
            + KV+D  + L
Sbjct: 587 IENKVVDFQELL 622


>TC81007 similar to GP|17473902|gb|AAL38369.1 RNA helicase - like protein
           {Arabidopsis thaliana}, partial (96%)
          Length = 1181

 Score = 27.3 bits (59), Expect = 5.9
 Identities = 16/53 (30%), Positives = 29/53 (54%), Gaps = 8/53 (15%)
 Frame = -3

Query: 100 DKSVLKRPYLTTQSERS--------GIYKPPKTRKKPNLEGIGSMKCNCPFRL 144
           ++SV+ + YL   S+ +        GI KP   R   ++EG GS++C+C  ++
Sbjct: 483 NRSVINQTYLHLSSKYTIFECIRIIGIPKPVH*R---SIEGFGSLRCHCDMKI 334


>TC87681 similar to GP|17381090|gb|AAL36357.1 unknown protein {Arabidopsis
           thaliana}, partial (29%)
          Length = 796

 Score = 26.9 bits (58), Expect = 7.7
 Identities = 21/65 (32%), Positives = 31/65 (47%)
 Frame = +2

Query: 152 TNDWWLAILCGMHNHDLEEKLQGHLIAGRLSAEEKKKVIDMTKSLTVPRNILTNLKENNK 211
           T  W L IL  +H+H L+  LQ    A  +  E K  ++++T S  V    + NL    K
Sbjct: 20  TQPWLLTILSLLHHHLLQSLLQNQ--ACFIMVEVKISMMNLTSSKLV--IFVENLLAKTK 187

Query: 212 ESVTT 216
            S+ T
Sbjct: 188 TSLCT 202


>TC83355 similar to GP|6016726|gb|AAF01552.1| unknown protein {Arabidopsis
           thaliana}, partial (68%)
          Length = 1032

 Score = 26.9 bits (58), Expect = 7.7
 Identities = 12/41 (29%), Positives = 20/41 (48%)
 Frame = +2

Query: 156 WLAILCGMHNHDLEEKLQGHLIAGRLSAEEKKKVIDMTKSL 196
           W  I+ G  NH   +  + H+   RL     KKV+ ++K +
Sbjct: 110 WGMIILGTDNHQESQVSRTHIQISRLCTSVSKKVLVLSKKI 232


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.318    0.136    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,046,075
Number of Sequences: 36976
Number of extensions: 107688
Number of successful extensions: 576
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 563
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 576
length of query: 256
length of database: 9,014,727
effective HSP length: 94
effective length of query: 162
effective length of database: 5,538,983
effective search space: 897315246
effective search space used: 897315246
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)


Medicago: description of AC135234.5