BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135232.5 + phase: 0
(812 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC88055 similar to GP|4151068|emb|CAA71816.1 ribonucleotide redu... 943 0.0
TC80353 similar to GP|4151068|emb|CAA71816.1 ribonucleotide redu... 551 e-157
BF004346 similar to GP|15451586|gb Putative ribonucleotide reduc... 229 3e-62
BQ135363 homologue to GP|5091526|dbj| ESTs C27722(C52692) AU0580... 71 1e-12
BQ135955 38 0.014
BF003394 similar to EGAD|118799|127 hypothetical protein F31B9.d... 30 3.7
TC81668 similar to GP|21618160|gb|AAM67210.1 unknown {Arabidopsi... 29 8.3
BE940956 29 8.3
>TC88055 similar to GP|4151068|emb|CAA71816.1 ribonucleotide reductase
{Nicotiana tabacum}, partial (57%)
Length = 1465
Score = 943 bits (2437), Expect = 0.0
Identities = 463/463 (100%), Positives = 463/463 (100%)
Frame = +1
Query: 3 VVKRDGRQEAVHFDKITARLKKLSYGLSTEHCDPVLVAQKVCAGVYKGVTTSQLDELAAE 62
VVKRDGRQEAVHFDKITARLKKLSYGLSTEHCDPVLVAQKVCAGVYKGVTTSQLDELAAE
Sbjct: 76 VVKRDGRQEAVHFDKITARLKKLSYGLSTEHCDPVLVAQKVCAGVYKGVTTSQLDELAAE 255
Query: 63 TAAGMTANHPDYASLAARIAVSNLHKNTLKSFSETVKVMYNHFNHRSGKKAPLIADDVYE 122
TAAGMTANHPDYASLAARIAVSNLHKNTLKSFSETVKVMYNHFNHRSGKKAPLIADDVYE
Sbjct: 256 TAAGMTANHPDYASLAARIAVSNLHKNTLKSFSETVKVMYNHFNHRSGKKAPLIADDVYE 435
Query: 123 IIIKNAARLDSEIIYDRDFDYDYFGFKTLERSYLLKVQGVVVERPQHMIMRVSVGIHKDD 182
IIIKNAARLDSEIIYDRDFDYDYFGFKTLERSYLLKVQGVVVERPQHMIMRVSVGIHKDD
Sbjct: 436 IIIKNAARLDSEIIYDRDFDYDYFGFKTLERSYLLKVQGVVVERPQHMIMRVSVGIHKDD 615
Query: 183 VESAVKTYHFMSQRWFTHASPTLFNAGTPRPQLSSCFLVCMKEDSIEGIYDTLKECAVIS 242
VESAVKTYHFMSQRWFTHASPTLFNAGTPRPQLSSCFLVCMKEDSIEGIYDTLKECAVIS
Sbjct: 616 VESAVKTYHFMSQRWFTHASPTLFNAGTPRPQLSSCFLVCMKEDSIEGIYDTLKECAVIS 795
Query: 243 KSAGGIGVSVHNIRAHGSYIRGTNGTSNGIVPMLRVFNDTARYVDQGGGKRKGAFAVYLE 302
KSAGGIGVSVHNIRAHGSYIRGTNGTSNGIVPMLRVFNDTARYVDQGGGKRKGAFAVYLE
Sbjct: 796 KSAGGIGVSVHNIRAHGSYIRGTNGTSNGIVPMLRVFNDTARYVDQGGGKRKGAFAVYLE 975
Query: 303 PWHADIFEFLELKINHGKEEQRARDLFYALWVPDLFMERVQSNGEWSLFCPNEAPGLADC 362
PWHADIFEFLELKINHGKEEQRARDLFYALWVPDLFMERVQSNGEWSLFCPNEAPGLADC
Sbjct: 976 PWHADIFEFLELKINHGKEEQRARDLFYALWVPDLFMERVQSNGEWSLFCPNEAPGLADC 1155
Query: 363 WGEEYDKLYTQYEKAGKAKKIVQAQSLWFAILKSQIETGNPYMLFKDACNRKSNQQNLGT 422
WGEEYDKLYTQYEKAGKAKKIVQAQSLWFAILKSQIETGNPYMLFKDACNRKSNQQNLGT
Sbjct: 1156WGEEYDKLYTQYEKAGKAKKIVQAQSLWFAILKSQIETGNPYMLFKDACNRKSNQQNLGT 1335
Query: 423 IKSSNLCTEIIEYTSPTETAVCNLASIALPRYVRDKGLPVESH 465
IKSSNLCTEIIEYTSPTETAVCNLASIALPRYVRDKGLPVESH
Sbjct: 1336IKSSNLCTEIIEYTSPTETAVCNLASIALPRYVRDKGLPVESH 1464
>TC80353 similar to GP|4151068|emb|CAA71816.1 ribonucleotide reductase
{Nicotiana tabacum}, partial (31%)
Length = 1159
Score = 551 bits (1421), Expect = e-157
Identities = 269/271 (99%), Positives = 270/271 (99%)
Frame = +3
Query: 542 EAQQLNKDIFETIYYHALKTSCELAAKEGPYETYSGSPVSKGILQPDMWGVTPSNLWDWG 601
+AQQLNKDIFETIYYHALKTS ELAAKEGPYETYSGSPVSKGILQPDMWGVTPSNLWDWG
Sbjct: 3 QAQQLNKDIFETIYYHALKTSWELAAKEGPYETYSGSPVSKGILQPDMWGVTPSNLWDWG 182
Query: 602 ALREMISKTGVRNSLLVAPMPTASTSQILGNNECFEPYTSNIYNRRVLSGEFVVVNKHLL 661
ALREMISKTGVRNSLLVAPMPTASTSQILGNNECFEPYTSNIYNRRVLSGEFVVVNKHLL
Sbjct: 183 ALREMISKTGVRNSLLVAPMPTASTSQILGNNECFEPYTSNIYNRRVLSGEFVVVNKHLL 362
Query: 662 HDLTEMGLWNPALKNKIIYDNGSVQNLSEIPAELKVIYKTVWEIKQKILVDMAVDRGCYI 721
HDLTEMGLWNPALKNKIIYDNGSVQNLSEIPAELKVIYKTVWEIKQKILVDMAVDRGCYI
Sbjct: 363 HDLTEMGLWNPALKNKIIYDNGSVQNLSEIPAELKVIYKTVWEIKQKILVDMAVDRGCYI 542
Query: 722 DQSQSLNIHMDKPNFGKLTSLHFYAWSKGLKTGMYYLRTRAASDAIKFTVDTSAIKDQPK 781
DQSQSLNIHMDKPNFGKLTSLHFYAWSKGLKTGMYYLRTRAASDAIKFTVDTSAIKDQPK
Sbjct: 543 DQSQSLNIHMDKPNFGKLTSLHFYAWSKGLKTGMYYLRTRAASDAIKFTVDTSAIKDQPK 722
Query: 782 VKEADDEDDDTKMAQMVCSLTNRDECEACGS 812
VKEADDEDDDTKMAQMVCSLTNRDECEACGS
Sbjct: 723 VKEADDEDDDTKMAQMVCSLTNRDECEACGS 815
>BF004346 similar to GP|15451586|gb Putative ribonucleotide reductase {Oryza
sativa} [Oryza sativa (japonica cultivar-group)],
partial (50%)
Length = 548
Score = 229 bits (583), Expect(2) = 3e-62
Identities = 114/129 (88%), Positives = 119/129 (91%), Gaps = 3/129 (2%)
Frame = +2
Query: 687 NLSEIPAELKVIYKTVWEIKQKILVDMAVDRGCYIDQSQSLNIHMDKPNFGKLTSLHFYA 746
NLSEI AE K IYKTVWEIKQKILV+MAVDRGCYIDQSQSLNIHMD+P +G LTSLHF+A
Sbjct: 50 NLSEIGAERKGIYKTVWEIKQKILVNMAVDRGCYIDQSQSLNIHMDQPTYGMLTSLHFHA 229
Query: 747 WSKGLKTGMYYLRTRAASDAIKFTVDTSAIKDQPKVKEAD---DEDDDTKMAQMVCSLTN 803
WSKGLKTGMYYLRTRAASDAIKFTVDTSAIKDQPKVKEAD DEDDD KMAQMVCSLTN
Sbjct: 230 WSKGLKTGMYYLRTRAASDAIKFTVDTSAIKDQPKVKEADDAEDEDDDAKMAQMVCSLTN 409
Query: 804 RDECEACGS 812
RDEC +CGS
Sbjct: 410 RDECTSCGS 436
Score = 29.3 bits (64), Expect(2) = 3e-62
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +1
Query: 671 NPALKNKIIYDNGSV 685
NPALKN+I+YD GSV
Sbjct: 1 NPALKNEILYDYGSV 45
>BQ135363 homologue to GP|5091526|dbj| ESTs C27722(C52692) AU058088(S0509)
correspond to a region of the predicted gene.~Similar
to, partial (4%)
Length = 746
Score = 71.2 bits (173), Expect = 1e-12
Identities = 48/106 (45%), Positives = 58/106 (54%), Gaps = 5/106 (4%)
Frame = +1
Query: 257 AHGSYIRGTNGT---SNGIVPMLRVFNDTARYVDQGGGKRKGAFAVYLEPWHADIFEFLE 313
AHG +I TNGT ++ + T D GK G F V LEPW+ADI E L+
Sbjct: 70 AHGKHIP*TNGTV*RPCSYAACVQ*YCPTWGTSDDATGK--GHFGVILEPWYADILELLK 243
Query: 314 LKINHGKEEQRARDLFYAL--WVPDLFMERVQSNGEWSLFCPNEAP 357
KI EEQ ARDLFY+L + L+ E GEWSLFCP+E P
Sbjct: 244 YKIVPRMEEQHARDLFYSLLGCLISLWNES-HITGEWSLFCPSETP 378
>BQ135955
Length = 849
Score = 38.1 bits (87), Expect = 0.014
Identities = 42/136 (30%), Positives = 63/136 (45%), Gaps = 11/136 (8%)
Frame = +3
Query: 33 HCDPVLVAQKVCAGVYKGVTTSQLDELAAETAAGMTANHPD-YASLAARIAVSNLHKNTL 91
H P L QK+ +YK + + Q+ +L+ E P Y++ AA IAV N N +
Sbjct: 177 HIHPFLFRQKI*TSIYKRLFSYQIAQLSGEPELRCDR*PPAAYSTCAATIAV*NHVNNPM 356
Query: 92 KSFSETVKVMYNH-----FNHRSGKKAPLIADDVYEIIIKNAARLDSEIIY-DRDFDYD- 144
K F TVKV+ N ++S K LI I+ NA +++IY + F+
Sbjct: 357 KYFL*TVKVL*NPKISYVLENKS*KDC*LIVS--LNSILPNAN--PNKLIYINYKFEISP 524
Query: 145 ---YFGFKTLERSYLL 157
GFK+LE + L
Sbjct: 525 LDVLLGFKSLENKFFL 572
>BF003394 similar to EGAD|118799|127 hypothetical protein F31B9.d
{Caenorhabditis elegans}, partial (6%)
Length = 704
Score = 30.0 bits (66), Expect = 3.7
Identities = 18/47 (38%), Positives = 26/47 (55%)
Frame = +3
Query: 218 CFLVCMKEDSIEGIYDTLKECAVISKSAGGIGVSVHNIRAHGSYIRG 264
C+ C+ +EG++D L E + S G+G+ V A GSYIRG
Sbjct: 201 CWWCCVVVPLLEGLWDCLGEVWMDSCGCFGLGL-VSERFADGSYIRG 338
>TC81668 similar to GP|21618160|gb|AAM67210.1 unknown {Arabidopsis
thaliana}, partial (3%)
Length = 655
Score = 28.9 bits (63), Expect = 8.3
Identities = 15/52 (28%), Positives = 23/52 (43%)
Frame = +1
Query: 62 ETAAGMTANHPDYASLAARIAVSNLHKNTLKSFSETVKVMYNHFNHRSGKKA 113
E + A PD S+A + V + + SF +YN F HR+ +A
Sbjct: 427 EEKLNLAAESPDRKSMATSVEVQTGNSTEVFSFKGEKVSIYNSFQHRNRTRA 582
>BE940956
Length = 397
Score = 28.9 bits (63), Expect = 8.3
Identities = 18/49 (36%), Positives = 26/49 (52%), Gaps = 1/49 (2%)
Frame = -2
Query: 603 LREMISKTGVRNSL-LVAPMPTASTSQILGNNECFEPYTSNIYNRRVLS 650
L I ++ RNS ++ +P +T I NNE P T N++N VLS
Sbjct: 150 LSNSIPRSQYRNSSSIMGTLPHFNTFNIFSNNEQPIPTTFNLHNHTVLS 4
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.134 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,461,204
Number of Sequences: 36976
Number of extensions: 329060
Number of successful extensions: 1498
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1488
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1495
length of query: 812
length of database: 9,014,727
effective HSP length: 104
effective length of query: 708
effective length of database: 5,169,223
effective search space: 3659809884
effective search space used: 3659809884
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)
Medicago: description of AC135232.5