BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135230.12 + phase: 0
(1373 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB894097 homologue to PIR|T47523|T4 DNA-binding protein-like - A... 223 3e-58
AW329600 112 1e-24
TC78122 similar to GP|21593751|gb|AAM65718.1 unknown {Arabidopsi... 42 0.002
BF632358 weakly similar to GP|19352296|gb MULTIPLE BANDED ANTIGE... 36 0.12
TC91570 weakly similar to GP|7208782|emb|CAB76913.1 hypothetical... 33 0.58
TC81158 similar to GP|9280664|gb|AAF86533.1| F21B7.15 {Arabidops... 33 0.58
TC78206 similar to GP|15450507|gb|AAK96546.1 AT4g27600/T29A15_90... 33 0.58
AW257087 homologue to GP|6683126|dbj KIAA0339 protein {Homo sapi... 32 1.7
CB892018 32 2.2
TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - ... 32 2.2
BF645940 31 2.9
TC88618 weakly similar to PIR|T48397|T48397 S-receptor kinase-li... 31 2.9
TC80397 similar to PIR|H71404|H71404 hypothetical protein d13195... 31 3.8
BQ750900 similar to GP|295671|gb|AA selected as a weak suppresso... 31 3.8
BI308703 similar to GP|6466949|gb|A unknown protein {Arabidopsis... 30 6.4
TC88059 similar to GP|12322736|gb|AAG51360.1 putative protein ki... 30 6.4
TC93070 weakly similar to GP|17065230|gb|AAL32769.1 Unknown prot... 30 6.4
TC82216 similar to GP|16930419|gb|AAL31895.1 AT4g21800/F17L22_26... 30 6.4
BF647566 similar to GP|9294624|dbj DNA repair protein RAD54-like... 30 6.4
AW586816 30 6.4
>CB894097 homologue to PIR|T47523|T4 DNA-binding protein-like - Arabidopsis
thaliana, partial (8%)
Length = 344
Score = 223 bits (569), Expect = 3e-58
Identities = 111/114 (97%), Positives = 112/114 (97%)
Frame = +2
Query: 1083 RAQKSLNTISPSSEEVRAYFRKEELLRYSIPDRAFSYTAADGKKSIVAPLRRCGGKPTSK 1142
RAQKSLNTISPSSEEVRAYFRKEELLRYSIPDRAFSYT ADGKKSIVAPLRRCGGKPTSK
Sbjct: 2 RAQKSLNTISPSSEEVRAYFRKEELLRYSIPDRAFSYTGADGKKSIVAPLRRCGGKPTSK 181
Query: 1143 ARDHFMLKRDRPPHVTILCLVRDAAARLPGSIGTRADVCTLIRDSQYIVEDVSD 1196
AR+HFMLKRDRPPHV ILCLVRDAAARLPGSIGTRADVCTLIRDSQYIVEDVSD
Sbjct: 182 AREHFMLKRDRPPHVKILCLVRDAAARLPGSIGTRADVCTLIRDSQYIVEDVSD 343
>AW329600
Length = 183
Score = 112 bits (279), Expect = 1e-24
Identities = 52/52 (100%), Positives = 52/52 (100%)
Frame = +3
Query: 1289 PPCIEDDKEAVQLLTTDTRPNAEDQVVVNPVSEVGNSCEDNSMTWEALDLNP 1340
PPCIEDDKEAVQLLTTDTRPNAEDQVVVNPVSEVGNSCEDNSMTWEALDLNP
Sbjct: 27 PPCIEDDKEAVQLLTTDTRPNAEDQVVVNPVSEVGNSCEDNSMTWEALDLNP 182
>TC78122 similar to GP|21593751|gb|AAM65718.1 unknown {Arabidopsis
thaliana}, partial (28%)
Length = 1394
Score = 42.0 bits (97), Expect = 0.002
Identities = 44/183 (24%), Positives = 71/183 (38%), Gaps = 6/183 (3%)
Frame = +3
Query: 551 GSHMLARG---EETESDSSEQWDDDDDNNPLLQSKFAYPIGKAAGSLTKPLKSHLDPMKA 607
G H+ G E ES SS DD DN+ +QSK GS L S D +
Sbjct: 330 GRHLFFTGGVSSEPESSSSIGTPDDSDNDEEVQSKLNLKGRNGLGS----LDSLEDSLPI 497
Query: 608 KFSRTDMKATQSKKIGGFAEQGNMHGADNYLSKNAKKSKIFNGSPVRNPAGKFMEENYPS 667
K + +SK ++ N++ S K+ ++ S + + N S
Sbjct: 498 KRGLSSHFEGKSKSFTDLSQVSNLNELQKQESPFNKRRRVLIASKFSRKSSFYSWSNPQS 677
Query: 668 VSDM-LNGGHDDWRQLYKSKNDQ--IRDEPVQRFDMPSSTSYAAEHKKKGRIGLDHSSMR 724
+ + +N DD+ +Y D+ R P SS S A + K++ ++ + H M
Sbjct: 678 MPLLPVNEDRDDYDDVYYDYEDEEKARKMPSGSSSSSSSNSLADDKKQEDQVQIGHGRMH 857
Query: 725 SKY 727
Y
Sbjct: 858 ESY 866
>BF632358 weakly similar to GP|19352296|gb MULTIPLE BANDED ANTIGEN
(FRAGMENT). 6/101 {Dictyostelium discoideum}, partial
(3%)
Length = 578
Score = 35.8 bits (81), Expect = 0.12
Identities = 20/64 (31%), Positives = 34/64 (52%), Gaps = 4/64 (6%)
Frame = +2
Query: 3 IEKN--SFKVSRVDTECE--PMSKESMSSGDEEDVQRRNSGNESDEDDDEFDDADSGAGS 58
IE N FK+ + + CE P+ ++ ++ ED + ++ D DD++ DA+ G S
Sbjct: 332 IENNVFKFKILELSSMCEKMPVMEDEENNNMMEDEEEEKDSDDVDYDDNDDHDAEEGNDS 511
Query: 59 DDFD 62
DD D
Sbjct: 512 DDDD 523
>TC91570 weakly similar to GP|7208782|emb|CAB76913.1 hypothetical protein
{Cicer arietinum}, partial (17%)
Length = 1446
Score = 33.5 bits (75), Expect = 0.58
Identities = 41/190 (21%), Positives = 76/190 (39%), Gaps = 6/190 (3%)
Frame = +2
Query: 659 KFMEENYPSVSDMLNGGHDDWRQLYKSKNDQIRDEPVQRFDMPSSTSYAAEHK------K 712
K++EEN NGG + + L K ++ D+P + + + + E K
Sbjct: 98 KYLEENNDQPKKNSNGGAKEEKVLGKKTLEEKNDKPKKSLNGSAKDEKSLEQKTLEENND 277
Query: 713 KGRIGLDHSSMRSKYLHDYGNDEDDSLENRLLADENGVGQSRFWRKGQKNVAHKDDRDER 772
K + L+ S+ K L +E++ + L GV K +K++ K ++E
Sbjct: 278 KPKKSLNGSAKEEKSLGQKTPEENNDKPKKSL--NGGV-------KEEKDLGQK-TQEEN 427
Query: 773 SEVPLLGCNSAMKKRKMKFGAADFGERDEDANLLSSNPSKIDDLPAFSLKRKSKKKPGAE 832
++ P N +K+ K D G++ ++ N D P SL +K++ G
Sbjct: 428 NDKPKKSLNGGVKEEK------DLGQKTQENN----------DKPKKSLSGGAKEEKGLG 559
Query: 833 MVISEMENSE 842
E N +
Sbjct: 560 QKAQEENNGK 589
Score = 32.3 bits (72), Expect = 1.3
Identities = 48/207 (23%), Positives = 76/207 (36%), Gaps = 8/207 (3%)
Frame = +2
Query: 620 KKIGGFAEQGNMHGADNYLSKNAKKSKIFNGSPVRNPA--GKFMEENYPSVSDMLNGGHD 677
K + G A+ N K K NGS + K EEN LNGG
Sbjct: 209 KSLNGSAKDEKSLEQKTLEENNDKPKKSLNGSAKEEKSLGQKTPEENNDKPKKSLNGGVK 388
Query: 678 DWRQLYKSKNDQIRDEPVQRFDMPSSTSYAAEHKKKGRIGLDHSSMRSKYLHDYGNDEDD 737
+ + L + ++ D+P + S E K G+ +++ K L G E+
Sbjct: 389 EEKDLGQKTQEENNDKPKK-----SLNGGVKEEKDLGQKTQENNDKPKKSLSG-GAKEEK 550
Query: 738 SLENRLLADENGVGQSRF--WRKGQKNVAHKDDRDERSEVPLLGCNSAMKKRKMKFGAAD 795
L + + NG + K +K + K E P N+ ++ K G +
Sbjct: 551 GLGQKAQEENNGKPKKSLNGSAKEEKGLRQKALEQEGHNDP----NAVLQTSMKKVGDEE 718
Query: 796 FGERDEDAN----LLSSNPSKIDDLPA 818
G ED N ++S+N + LPA
Sbjct: 719 IGV-SEDLNGGDKIISNNYQRRASLPA 796
Score = 31.6 bits (70), Expect = 2.2
Identities = 42/199 (21%), Positives = 78/199 (39%), Gaps = 3/199 (1%)
Frame = +2
Query: 620 KKIGGFAEQGNMHGADNYLSKNAKKSKIFNGSPV--RNPAGKFMEENYPSVSDMLNGGHD 677
K G A++ + G KN K K NGS ++ K +EEN LNG
Sbjct: 131 KNSNGGAKEEKVLGKKTLEEKNDKPKKSLNGSAKDEKSLEQKTLEENNDKPKKSLNGSAK 310
Query: 678 DWRQLYKSKNDQIRDEPVQRFDMPSSTSYAAEHKKKGRIGLDHSSMRSKYLHDYGNDEDD 737
+ + L + ++ D+P + S E K G+ + ++ + K + G E+
Sbjct: 311 EEKSLGQKTPEENNDKPKK-----SLNGGVKEEKDLGQKTQEENNDKPKKSLNGGVKEEK 475
Query: 738 SLENRLLADENGVGQSRFWRKGQKNVAHKDDRDERSEVPLLGCNSAMKKRKMKFGAADFG 797
L + + + +S ++ + ++E + P N + K+ K A
Sbjct: 476 DLGQKTQENNDKPKKSLSGGAKEEKGLGQKAQEENNGKPKKSLNGSAKEEKGLRQKALEQ 655
Query: 798 ERDEDAN-LLSSNPSKIDD 815
E D N +L ++ K+ D
Sbjct: 656 EGHNDPNAVLQTSMKKVGD 712
>TC81158 similar to GP|9280664|gb|AAF86533.1| F21B7.15 {Arabidopsis
thaliana}, partial (6%)
Length = 890
Score = 33.5 bits (75), Expect = 0.58
Identities = 20/60 (33%), Positives = 31/60 (51%), Gaps = 1/60 (1%)
Frame = +1
Query: 2 AIEKNSFKVSRVDTECEPMSKESMSSGDEEDVQRRNSGNESDEDD-DEFDDADSGAGSDD 60
++E N + +E E S+ S S + SG++SDEDD D+ +D + G SDD
Sbjct: 382 SVEVNGNEDKDDGSEGESESESSESESESSTSSSSTSGSDSDEDDEDDAEDEEEGQISDD 561
>TC78206 similar to GP|15450507|gb|AAK96546.1 AT4g27600/T29A15_90
{Arabidopsis thaliana}, partial (76%)
Length = 1677
Score = 33.5 bits (75), Expect = 0.58
Identities = 26/82 (31%), Positives = 41/82 (49%), Gaps = 10/82 (12%)
Frame = +3
Query: 2 AIEKNSFKVSRVDT-----ECEPMSKESMSSGDEEDVQRRNSGNESDEDDDEFDDADSGA 56
+I++ FKV T E E MS+ S +EED +++ DED+ E DD + G
Sbjct: 285 SIQRGVFKVCSFSTFGGGAEFESMSQRSHD--EEEDDEQQIRTRAGDEDEVEDDDEEEGC 458
Query: 57 GS-----DDFDLLELGETGAEF 73
S + +D+L LG+ +F
Sbjct: 459 VSSFVFPERWDVLGLGQAMVDF 524
>AW257087 homologue to GP|6683126|dbj KIAA0339 protein {Homo sapiens},
partial (2%)
Length = 466
Score = 32.0 bits (71), Expect = 1.7
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = -3
Query: 40 NESDEDDDEFDDADSGAGSDDFDLLELGETGAE 72
++ DED+DE +D D DD +LLE E+ +E
Sbjct: 380 DDEDEDEDEDEDEDEDEDEDDEELLEESESESE 282
>CB892018
Length = 389
Score = 31.6 bits (70), Expect = 2.2
Identities = 28/96 (29%), Positives = 41/96 (42%), Gaps = 5/96 (5%)
Frame = +1
Query: 709 EHKKKGRIGLDHSSMRSKYLHDYGNDEDDSLENRLLADENGVGQSRF-----WRKGQKNV 763
E KK GR D + K DE+D E + L DE+ G+ R + G+K++
Sbjct: 13 EEKKHGRNEDDSRDGKHK------RDEEDRREKKSLRDEDVRGERRHGKDEDYPTGRKHL 174
Query: 764 AHKDDRDERSEVPLLGCNSAMKKRKMKFGAADFGER 799
+DD ER + +RK D+GER
Sbjct: 175 RDEDDHGERK---IRRIEDEHAERKSLRDGDDYGER 273
>TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - alfalfa,
partial (69%)
Length = 1615
Score = 31.6 bits (70), Expect = 2.2
Identities = 17/56 (30%), Positives = 30/56 (53%), Gaps = 3/56 (5%)
Frame = +3
Query: 18 EPMSKESMSSGDEEDVQRRNSGNESDEDDDEFDDADSGAGS---DDFDLLELGETG 70
E ES SS DE+ + G++SDE ++E +D S D ++++ G++G
Sbjct: 333 EVSESESDSSDDEDKMNVDKDGSDSDESEEESEDEPSKTPQKKIKDVEMVDAGKSG 500
>BF645940
Length = 507
Score = 31.2 bits (69), Expect = 2.9
Identities = 28/115 (24%), Positives = 46/115 (39%)
Frame = -2
Query: 397 NLMGLSMSSKTDLRGYTRNPNQSSDMQLFAAKPPSKKKGKYAENVQQFVGSRGSKLSHNV 456
NL LS KT ++P Q + F + K K + ++ SK ++
Sbjct: 401 NLFHLSQKKKTQ-----QSPKQLQILTFFTSS-----KNKITKTTSKYKLFSPSK---SI 261
Query: 457 DSIHSPDPDDLFYNKRPAQELGMSSLFKYEDWNPKSKKRKAERESPDLSYTAYRS 511
++HSP + KRP + L K NP++K K E P ++ R+
Sbjct: 260 PALHSPQQ----FTKRPRNTNHYNKLNKTHQSNPRTKTSKGYEEKPYYTFLKIRN 108
>TC88618 weakly similar to PIR|T48397|T48397 S-receptor kinase-like protein -
Arabidopsis thaliana, partial (38%)
Length = 2304
Score = 31.2 bits (69), Expect = 2.9
Identities = 22/75 (29%), Positives = 33/75 (43%)
Frame = +1
Query: 1267 TVACNGTGEQSGYDLCSDLNVDPPCIEDDKEAVQLLTTDTRPNAEDQVVVNPVSEVGNSC 1326
T A G GE++ Y + + V+PP KE ++ +TT + ++E C
Sbjct: 952 TYAVGGIGEENSYKILRKVGVEPP----HKELLEEVTTLS------------LTECEGLC 1083
Query: 1327 EDNSMTWEALDLNPT 1341
E N W AL N T
Sbjct: 1084 EKNCKCWGALYNNQT 1128
>TC80397 similar to PIR|H71404|H71404 hypothetical protein d13195c -
Arabidopsis thaliana, partial (16%)
Length = 1636
Score = 30.8 bits (68), Expect = 3.8
Identities = 15/57 (26%), Positives = 31/57 (54%)
Frame = +1
Query: 1220 FDQERKLWVYLHREREEEDFDDDGTSSTKKWKRQKKDVADQSDQAPVTVACNGTGEQ 1276
F+Q+ + + L + + E+FDD G + + ++ +AD++D+A NG G +
Sbjct: 13 FNQKCDIDMVLEADEKLEEFDDQGKENNNNTQEEEGFIADETDEA-FNFKLNGIGNK 180
>BQ750900 similar to GP|295671|gb|AA selected as a weak suppressor of a
mutant of the subunit AC40 of DNA dependant RNA
polymerase I and, partial (6%)
Length = 737
Score = 30.8 bits (68), Expect = 3.8
Identities = 19/41 (46%), Positives = 23/41 (55%)
Frame = -3
Query: 20 MSKESMSSGDEEDVQRRNSGNESDEDDDEFDDADSGAGSDD 60
+ E M+SGDEE G ESD+DD+ D DS SDD
Sbjct: 561 LDDEEMTSGDEE-------GAESDDDDEPSDKQDS---SDD 469
>BI308703 similar to GP|6466949|gb|A unknown protein {Arabidopsis thaliana},
partial (4%)
Length = 750
Score = 30.0 bits (66), Expect = 6.4
Identities = 15/65 (23%), Positives = 28/65 (43%)
Frame = +3
Query: 438 AENVQQFVGSRGSKLSHNVDSIHSPDPDDLFYNKRPAQELGMSSLFKYEDWNPKSKKRKA 497
A+N + S + ++H S+ P + YN R Q++G + ++W PK
Sbjct: 243 AKNHGNEIPSPSTPVTHGSPSVSRPSSN---YNNRSQQQVGSQKVGSNKEWKPKPTNTSN 413
Query: 498 ERESP 502
+ P
Sbjct: 414 QNSGP 428
>TC88059 similar to GP|12322736|gb|AAG51360.1 putative protein kinase;
70907-69052 {Arabidopsis thaliana}, partial (62%)
Length = 2063
Score = 30.0 bits (66), Expect = 6.4
Identities = 28/141 (19%), Positives = 52/141 (36%), Gaps = 3/141 (2%)
Frame = +1
Query: 802 DANLLSSNPSKIDDLPAFSLKRKSKKKPGAEMVISEMENSELPLTHTVTADVEVETKPQK 861
D N +PS + + F++ ++ KKKP + + + E
Sbjct: 223 DKNRYL*HPSSVHRMGCFTILKRKKKKPDQIVYVKRVSPGE------------------D 348
Query: 862 KPYILITPTVHTGFSFSIMHLLTAVRTAMISPPEVESLEAGKPVEQQNKAQEDSLNGVIS 921
P +L P HT R+ +PP + KP++ NKA + + + +
Sbjct: 349 SPTVLPEPQTHT-------------RSLQSAPPSFKIRV--KPIQPSNKATNNRIRALSA 483
Query: 922 SDKVDD---KVAANVEPSDQK 939
+DD A +E +Q+
Sbjct: 484 PSSLDDAEQDALATIEYEEQE 546
>TC93070 weakly similar to GP|17065230|gb|AAL32769.1 Unknown protein
{Arabidopsis thaliana}, partial (19%)
Length = 679
Score = 30.0 bits (66), Expect = 6.4
Identities = 17/49 (34%), Positives = 23/49 (46%)
Frame = +1
Query: 14 DTECEPMSKESMSSGDEEDVQRRNSGNESDEDDDEFDDADSGAGSDDFD 62
D E E M E ++ED ++ D+DDDE DD D A D +
Sbjct: 226 DDEIEEMDFED---DEDEDEDEDEDDDDDDDDDDEDDDDDGFAEPTDLN 363
Score = 29.6 bits (65), Expect = 8.4
Identities = 13/41 (31%), Positives = 21/41 (50%)
Frame = +1
Query: 23 ESMSSGDEEDVQRRNSGNESDEDDDEFDDADSGAGSDDFDL 63
E M D+ED ++ D+DDD+ +D D ++ DL
Sbjct: 238 EEMDFEDDEDEDEDEDEDDDDDDDDDDEDDDDDGFAEPTDL 360
>TC82216 similar to GP|16930419|gb|AAL31895.1 AT4g21800/F17L22_260
{Arabidopsis thaliana}, partial (31%)
Length = 702
Score = 30.0 bits (66), Expect = 6.4
Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 6/71 (8%)
Frame = +2
Query: 4 EKNSFKVSRVDTECEPMSKESMSSGDEEDVQR---RNSGNESDEDDDEFDDADS---GAG 57
EK + +R + + +E SG E V +N + DE+D+E DD D+ G
Sbjct: 239 EKQLLEENRRKENMDKLRREMEKSGGETVVLSTGLKNKEEDEDEEDEEMDDDDNVDFGTY 418
Query: 58 SDDFDLLELGE 68
++D D ++ E
Sbjct: 419 TEDEDAIDEDE 451
>BF647566 similar to GP|9294624|dbj DNA repair protein RAD54-like
{Arabidopsis thaliana}, partial (12%)
Length = 669
Score = 30.0 bits (66), Expect = 6.4
Identities = 17/47 (36%), Positives = 24/47 (50%), Gaps = 1/47 (2%)
Frame = -3
Query: 14 DTECEPMS-KESMSSGDEEDVQRRNSGNESDEDDDEFDDADSGAGSD 59
D CE S ES +EED S +ES+E DD+ + S + S+
Sbjct: 217 DFRCETCSISESEEEEEEEDDDTSTSSSESEEKDDDDNKTTSSSSSN 77
>AW586816
Length = 624
Score = 30.0 bits (66), Expect = 6.4
Identities = 20/63 (31%), Positives = 29/63 (45%)
Frame = +1
Query: 1292 IEDDKEAVQLLTTDTRPNAEDQVVVNPVSEVGNSCEDNSMTWEALDLNPTRELCQENSTN 1351
+E + A LLT + +A + + P S G+S D+S TW N LC + N
Sbjct: 358 LELESRAYNLLTCTGKGSASIRSIEVPDSATGSSSIDSSKTWNV--CNSGNVLCCKVDKN 531
Query: 1352 EDF 1354
E F
Sbjct: 532 EKF 540
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.312 0.131 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,048,532
Number of Sequences: 36976
Number of extensions: 525491
Number of successful extensions: 2476
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 2169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2378
length of query: 1373
length of database: 9,014,727
effective HSP length: 108
effective length of query: 1265
effective length of database: 5,021,319
effective search space: 6351968535
effective search space used: 6351968535
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 64 (29.3 bits)
Medicago: description of AC135230.12