BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134521.7 + phase: 0
(183 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78972 weakly similar to GP|21592657|gb|AAM64606.1 unknown {Ara... 30 0.54
TC88281 similar to PIR|B84784|B84784 hypothetical protein At2g36... 30 0.54
AJ498285 weakly similar to GP|20799090|em ADP-ribosylation facto... 28 2.0
TC84424 similar to GP|23504658|emb|CAD51536. hypothetical protei... 28 2.0
TC77690 similar to PIR|E86385|E86385 probable transmembrane prot... 28 2.0
TC85700 weakly similar to GP|8978266|dbj|BAA98157.1 anthocyanidi... 28 2.7
TC83590 similar to GP|9758365|dbj|BAB08866.1 GTP binding protein... 27 3.5
TC87567 similar to GP|17065112|gb|AAL32710.1 Unknown protein {Ar... 27 3.5
BI308153 similar to GP|9294410|dbj protein kinase-like protein {... 27 3.5
TC78491 similar to SP|Q43068|C821_PEA Cytochrome P450 82A1 (EC 1... 27 3.5
TC82194 similar to PIR|T48601|T48601 hypothetical protein F18O22... 27 3.5
TC87399 similar to GP|17381168|gb|AAL36396.1 putative cellulose ... 27 4.5
BQ139153 similar to GP|19310589|gb unknown protein {Arabidopsis ... 27 4.5
TC86198 similar to GP|21536867|gb|AAM61199.1 putative long-chain... 27 4.5
BF640151 similar to PIR|D96495|D964 unknown protein [imported] -... 27 4.5
TC85811 similar to PIR|T09943|T09943 probable 12-oxophytodienoat... 27 4.5
TC86492 homologue to GP|11907554|dbj|BAB19676. expansin {Prunus ... 27 5.9
TC88040 homologue to SP|Q41266|AOX2_SOYBN Alternative oxidase 2 ... 27 5.9
TC86491 homologue to GP|11907554|dbj|BAB19676. expansin {Prunus ... 27 5.9
TC82355 similar to SP|P82538|TL26_ARATH Thylakoid lumenal 25.6 k... 27 5.9
>TC78972 weakly similar to GP|21592657|gb|AAM64606.1 unknown {Arabidopsis
thaliana}, partial (27%)
Length = 1396
Score = 30.0 bits (66), Expect = 0.54
Identities = 14/45 (31%), Positives = 25/45 (55%), Gaps = 2/45 (4%)
Frame = +2
Query: 9 NPILQSSTQSPICKPQKFPFSQPNTSKQLNY--QFTTLKPQASAK 51
+P+ + + KPQK +P +K NY + +TL+P A++K
Sbjct: 626 HPLPKPQPNQEVLKPQKLKIKEPKIAKGRNYNRKESTLRPHAASK 760
>TC88281 similar to PIR|B84784|B84784 hypothetical protein At2g36740
[imported] - Arabidopsis thaliana, partial (59%)
Length = 1086
Score = 30.0 bits (66), Expect = 0.54
Identities = 24/62 (38%), Positives = 29/62 (46%), Gaps = 3/62 (4%)
Frame = +2
Query: 32 NTSKQLNY--QFTTLKPQASAKGFSNTR-PPSTNKAKDDVPIKKNPNNKNDDDDIPKEVM 88
N K+L Y + T+ K + K SN PP + D K N NN DDD KEVM
Sbjct: 302 NKKKRLVYPGKTTSAKKKKKKKILSNLDDPPKIDGDDDQDDDKNNKNNNQDDD--AKEVM 475
Query: 89 YR 90
R
Sbjct: 476 IR 481
>AJ498285 weakly similar to GP|20799090|em ADP-ribosylation factor guanine
nucleotide-exchange factor 6 {Homo sapiens}, partial
(2%)
Length = 568
Score = 28.1 bits (61), Expect = 2.0
Identities = 16/65 (24%), Positives = 30/65 (45%), Gaps = 2/65 (3%)
Frame = +1
Query: 14 SSTQSPICKPQKFPFSQPNTSKQLNYQ--FTTLKPQASAKGFSNTRPPSTNKAKDDVPIK 71
S SP P + P P+ S +N + P ++ +++ PST ++ D P+
Sbjct: 151 SPASSPESLPPESPSQSPSMSPSMNMSPSMSPPFPTDASPSPASSPSPSTGESMSDNPVA 330
Query: 72 KNPNN 76
+P+N
Sbjct: 331 SSPSN 345
>TC84424 similar to GP|23504658|emb|CAD51536. hypothetical protein
{Plasmodium falciparum 3D7}, partial (3%)
Length = 644
Score = 28.1 bits (61), Expect = 2.0
Identities = 16/58 (27%), Positives = 28/58 (47%), Gaps = 9/58 (15%)
Frame = +3
Query: 55 NTRPPSTNKAKDDVPIKKNPNNKNDDDDIPKEVMYRI---------IGRILFSTLVPM 103
N P N+ + D +N ++D+DD+P+ + R+ + R L S LVP+
Sbjct: 57 NPNNPKPNEEEIDEHENENEEEEDDEDDVPQSPLSRLRDQKFKLETLSRRLSSELVPI 230
>TC77690 similar to PIR|E86385|E86385 probable transmembrane protein
[imported] - Arabidopsis thaliana, partial (95%)
Length = 1215
Score = 28.1 bits (61), Expect = 2.0
Identities = 17/53 (32%), Positives = 24/53 (45%)
Frame = +1
Query: 127 WMPFVTTLVFFGASGLGIAYGVLSTSLDAEREGSLLGFEEVEKNWDEMWQQEN 179
W +TTL+FFG +G+ S EG EVE D+ W+ +N
Sbjct: 340 WAHHITTLLFFG-------FGLWSLKEAIFGEGESEELAEVEAELDKDWKAKN 477
>TC85700 weakly similar to GP|8978266|dbj|BAA98157.1
anthocyanidin-3-glucoside rhamnosyltransferase-like
{Arabidopsis thaliana}, partial (9%)
Length = 1179
Score = 27.7 bits (60), Expect = 2.7
Identities = 12/31 (38%), Positives = 18/31 (57%)
Frame = +1
Query: 104 ALGLSFLHLYGELKDRHIFNAPLWMPFVTTL 134
A L F + GE D +++ P W+PF TT+
Sbjct: 469 ARNLVFFNPPGERTDIDLYSPPKWVPFQTTI 561
>TC83590 similar to GP|9758365|dbj|BAB08866.1 GTP binding protein-like
{Arabidopsis thaliana}, partial (49%)
Length = 1061
Score = 27.3 bits (59), Expect = 3.5
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +3
Query: 58 PPSTNKAKDDVPIKKNPNNKNDDDD 82
PP+ ++ K +KK +KNDDD+
Sbjct: 165 PPTPDEDKSTTKLKKKKKDKNDDDN 239
>TC87567 similar to GP|17065112|gb|AAL32710.1 Unknown protein {Arabidopsis
thaliana}, partial (82%)
Length = 2117
Score = 27.3 bits (59), Expect = 3.5
Identities = 38/145 (26%), Positives = 57/145 (39%), Gaps = 12/145 (8%)
Frame = +2
Query: 32 NTSKQLNYQFTTLKPQASAKGFSNTR------PPSTNKAKDDVPIKKNPNNKNDDDDIPK 85
+ S Q+N F + + + S R P+T KD +N NN +DDD+ K
Sbjct: 173 SVSGQINTTFLSYTKKQHTRSSSVQRLPPIAAAPNTRDGKDSKSNTQNNNNDDDDDNDDK 352
Query: 86 EVMYRIIGRILFSTLVPMALGLSFLHLYGELKDRHIFNAPLWMPFVTTLVFFGASGLGIA 145
+ + + + P+ LG K R IF+ +W F SGLG
Sbjct: 353 QSV-----SLSLDDVNPVGLG---------RKSRQIFD-EVWRKF---------SGLGQI 460
Query: 146 YGVLST----SLDA--EREGSLLGF 164
+ T +LDA REG + F
Sbjct: 461 SRTIRTDDVEALDALLVREGPMCEF 535
>BI308153 similar to GP|9294410|dbj protein kinase-like protein {Arabidopsis
thaliana}, partial (33%)
Length = 825
Score = 27.3 bits (59), Expect = 3.5
Identities = 19/63 (30%), Positives = 27/63 (42%)
Frame = +1
Query: 14 SSTQSPICKPQKFPFSQPNTSKQLNYQFTTLKPQASAKGFSNTRPPSTNKAKDDVPIKKN 73
SST P +P + P S P S L P A+ G S + PPS + + N
Sbjct: 130 SSTTPPPQQPPQTPPSLP*KSLTKPPSTPNLTPNAAPDGKSKSSPPSPTLSSLQSLVHMN 309
Query: 74 PNN 76
P++
Sbjct: 310 PHS 318
>TC78491 similar to SP|Q43068|C821_PEA Cytochrome P450 82A1 (EC 1.14.-.-)
(CYPLXXXII) (Fragment). [Garden pea] {Pisum sativum},
partial (59%)
Length = 1032
Score = 27.3 bits (59), Expect = 3.5
Identities = 13/35 (37%), Positives = 22/35 (62%)
Frame = +2
Query: 41 FTTLKPQASAKGFSNTRPPSTNKAKDDVPIKKNPN 75
F+ KP +++ +NTRP STN+ + P +NP+
Sbjct: 80 FSYQKPSYNSRVMANTRPSSTNEKYTNTP--QNPS 178
>TC82194 similar to PIR|T48601|T48601 hypothetical protein F18O22.70 -
Arabidopsis thaliana, partial (10%)
Length = 858
Score = 27.3 bits (59), Expect = 3.5
Identities = 17/57 (29%), Positives = 26/57 (44%)
Frame = +2
Query: 18 SPICKPQKFPFSQPNTSKQLNYQFTTLKPQASAKGFSNTRPPSTNKAKDDVPIKKNP 74
+P+ K P S PN+ + FT PQ+SA S+ P T + P+ + P
Sbjct: 17 TPLSSVTKTPKSDPNSPAV*SPSFTAHPPQSSAPXPSS--PTPTAASHPSTPLSRKP 181
>TC87399 similar to GP|17381168|gb|AAL36396.1 putative cellulose synthase
catalytic subunit {Arabidopsis thaliana}, partial (53%)
Length = 1829
Score = 26.9 bits (58), Expect = 4.5
Identities = 14/38 (36%), Positives = 20/38 (51%)
Frame = -2
Query: 4 LVFASNPILQSSTQSPICKPQKFPFSQPNTSKQLNYQF 41
L+ NP++ SST P KP FP ++ +QL F
Sbjct: 1417 LLCIDNPVITSSTGHPYFKPISFPHCVFSSYEQLARPF 1304
>BQ139153 similar to GP|19310589|gb unknown protein {Arabidopsis thaliana},
partial (31%)
Length = 673
Score = 26.9 bits (58), Expect = 4.5
Identities = 12/39 (30%), Positives = 20/39 (50%)
Frame = +3
Query: 31 PNTSKQLNYQFTTLKPQASAKGFSNTRPPSTNKAKDDVP 69
P+T +L + TT+ +S F++T PP + D P
Sbjct: 174 PSTESELPHSTTTIISHSSPPSFTSTPPPLEKENTFDTP 290
>TC86198 similar to GP|21536867|gb|AAM61199.1 putative long-chain acyl-CoA
synthetase {Arabidopsis thaliana}, partial (58%)
Length = 1654
Score = 26.9 bits (58), Expect = 4.5
Identities = 12/43 (27%), Positives = 20/43 (45%)
Frame = +2
Query: 19 PICKPQKFPFSQPNTSKQLNYQFTTLKPQASAKGFSNTRPPST 61
P P KFP ++S +NY +T + + + S + ST
Sbjct: 293 PFHHPHKFPHHHSSSSSSINYSYTPIFSSSQFRSSSCSTSQST 421
>BF640151 similar to PIR|D96495|D964 unknown protein [imported] - Arabidopsis
thaliana, partial (6%)
Length = 588
Score = 26.9 bits (58), Expect = 4.5
Identities = 15/42 (35%), Positives = 19/42 (44%)
Frame = +3
Query: 39 YQFTTLKPQASAKGFSNTRPPSTNKAKDDVPIKKNPNNKNDD 80
YQ T +A++ T P AKDD K NNKN +
Sbjct: 159 YQSHTTARRAASLALPRTSVPFVPSAKDDNDTAKLDNNKNSN 284
>TC85811 similar to PIR|T09943|T09943 probable 12-oxophytodienoate reductase
(EC 1.3.1.42) - Madagascar periwinkle, partial (94%)
Length = 1462
Score = 26.9 bits (58), Expect = 4.5
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +2
Query: 112 LYGELKDRHIFNAPLWMPFVTTLVFFGASGLGIAYGVLSTSL 153
+YG LK + + PLWM F+T V+F + +G+L L
Sbjct: 263 VYG-LKSKSMHGNPLWMLFMTKAVYF-----SVKFGMLEEFL 370
>TC86492 homologue to GP|11907554|dbj|BAB19676. expansin {Prunus persica},
partial (95%)
Length = 1257
Score = 26.6 bits (57), Expect = 5.9
Identities = 8/22 (36%), Positives = 14/22 (63%)
Frame = +3
Query: 158 EGSLLGFEEVEKNWDEMWQQEN 179
+GS G++ + +NW + WQ N
Sbjct: 753 KGSKTGWQAMSRNWGQNWQSNN 818
>TC88040 homologue to SP|Q41266|AOX2_SOYBN Alternative oxidase 2
mitochondrial precursor (EC 1.-.-.-). [Soybean] {Glycine
max}, partial (82%)
Length = 1678
Score = 26.6 bits (57), Expect = 5.9
Identities = 13/48 (27%), Positives = 21/48 (43%)
Frame = -3
Query: 31 PNTSKQLNYQFTTLKPQASAKGFSNTRPPSTNKAKDDVPIKKNPNNKN 78
PN S Q Y P +S++ + PPS VP+ K ++ +
Sbjct: 689 PNNSTQPRYSSFRNSPPSSSRSSRSISPPSLPPMASTVPLAKTSSSSS 546
>TC86491 homologue to GP|11907554|dbj|BAB19676. expansin {Prunus persica},
partial (95%)
Length = 1166
Score = 26.6 bits (57), Expect = 5.9
Identities = 8/22 (36%), Positives = 14/22 (63%)
Frame = +2
Query: 158 EGSLLGFEEVEKNWDEMWQQEN 179
+GS G++ + +NW + WQ N
Sbjct: 644 KGSKTGWQAMSRNWGQNWQSNN 709
>TC82355 similar to SP|P82538|TL26_ARATH Thylakoid lumenal 25.6 kDa protein
chloroplast precursor. [Mouse-ear cress] {Arabidopsis
thaliana}, partial (37%)
Length = 739
Score = 26.6 bits (57), Expect = 5.9
Identities = 19/71 (26%), Positives = 33/71 (45%), Gaps = 2/71 (2%)
Frame = +1
Query: 66 DDVPIKKNPNNKNDDDDI--PKEVMYRIIGRILFSTLVPMALGLSFLHLYGELKDRHIFN 123
++V + P +K D + P++V +I ++L P F L L ++HI N
Sbjct: 502 ENVSVTMIPTSKQDIKEFGSPEQVAATLIKKVLAP---PNQKQR*FKQLXVRLTEKHIIN 672
Query: 124 APLWMPFVTTL 134
+ W+ TTL
Sbjct: 673 SNSWLXLQTTL 705
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.134 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,722,066
Number of Sequences: 36976
Number of extensions: 77830
Number of successful extensions: 560
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 559
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 560
length of query: 183
length of database: 9,014,727
effective HSP length: 90
effective length of query: 93
effective length of database: 5,686,887
effective search space: 528880491
effective search space used: 528880491
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC134521.7