
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134049.15 + phase: 0 /pseudo
(300 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI309716 weakly similar to PIR|G96722|G9 hypothetical protein F2... 45 3e-05
CB893805 similar to GP|10177935|d copia-type polyprotein {Arabid... 33 7e-05
AW689768 weakly similar to GP|10177485|d polyprotein {Arabidopsi... 43 1e-04
BG587156 similar to PIR|G85055|G8 probable polyprotein [imported... 39 0.003
BG452576 weakly similar to GP|12005223|gb|A reverse transcriptas... 35 0.046
BE123913 weakly similar to GP|22093573|d polyprotein {Oryza sati... 31 0.51
CB893680 weakly similar to GP|1167523|db ORF(AA 1-1338) {Nicotia... 30 1.1
BG647636 29 2.5
TC83658 28 3.3
TC85814 similar to SP|P48631|FD62_SOYBN Omega-6 fatty acid desat... 28 3.3
TC82246 similar to PIR|T02580|T02580 hypothetical protein At2g39... 28 4.3
BF640635 27 7.3
TC93570 similar to GP|21553436|gb|AAM62529.1 unknown {Arabidopsi... 27 7.3
>BI309716 weakly similar to PIR|G96722|G9 hypothetical protein F20P5.25
[imported] - Arabidopsis thaliana, partial (10%)
Length = 744
Score = 45.1 bits (105), Expect = 3e-05
Identities = 22/44 (50%), Positives = 30/44 (68%)
Frame = +2
Query: 158 SSFLTSLGYAQSQSGHSLFTKIHNQKNFTVILIFVDNLILPGND 201
S L S GY QS S SLFTK + +FT +L++VD+++L GND
Sbjct: 86 SESLISFGYLQSSSDFSLFTKFKDS-SFTTLLVYVDDIVLAGND 214
>CB893805 similar to GP|10177935|d copia-type polyprotein {Arabidopsis
thaliana}, partial (14%)
Length = 778
Score = 32.7 bits (73), Expect(2) = 7e-05
Identities = 14/43 (32%), Positives = 24/43 (55%)
Frame = +3
Query: 159 SFLTSLGYAQSQSGHSLFTKIHNQKNFTVILIFVDNLILPGND 201
++ T G+ + H+LF K+ +I ++VD+LI GND
Sbjct: 525 AYFTKEGFEKCPYEHTLFVKLSEGGKILIISLYVDDLIFIGND 653
Score = 30.4 bits (67), Expect(2) = 7e-05
Identities = 12/27 (44%), Positives = 20/27 (73%)
Frame = +2
Query: 101 LHQLDVNDTFIHGELDEKVYMVVPPFY 127
++QLDV F++GEL+E+V++ P Y
Sbjct: 359 VYQLDVKSAFLYGELNEEVFVDQPQGY 439
>AW689768 weakly similar to GP|10177485|d polyprotein {Arabidopsis thaliana},
partial (9%)
Length = 675
Score = 43.1 bits (100), Expect = 1e-04
Identities = 36/122 (29%), Positives = 59/122 (47%), Gaps = 10/122 (8%)
Frame = +1
Query: 89 LLLAIATTQY*HLHQLDVNDTFIHGELDEKVYMVVPPFYSHHALNQVYTDS*SLFMVSIR 148
L+L IA T + Q+D+N+ F++G L E+VYM P Q + + + +
Sbjct: 337 LILTIAITYKWEIQQIDINNAFLNGFLQEEVYMSQP---------QGFEAANKSLVCKLN 489
Query: 149 SASYSL-PCPSSFLTSLGYAQSQSGHSLFTK--------IHNQKNFTVIL-IFVDNLILP 198
+ Y L P ++ L AQ Q G FTK I+NQ + L I+VD++++
Sbjct: 490 KSLYGLKQAPRAWYEXLTSAQIQFG---FTKSRCDPSLLIYNQNGACIYLXIYVDDILIT 660
Query: 199 GN 200
G+
Sbjct: 661 GS 666
>BG587156 similar to PIR|G85055|G8 probable polyprotein [imported] -
Arabidopsis thaliana, partial (17%)
Length = 618
Score = 38.5 bits (88), Expect = 0.003
Identities = 27/97 (27%), Positives = 46/97 (46%), Gaps = 8/97 (8%)
Frame = -1
Query: 89 LLLAIATTQY*HLHQLDVNDTFIHGELDEKVYMVVPPFYSHHALNQVYTDS*SLFMVSIR 148
++L++A L Q+DV + F+ GEL+++VYM PP H ++ ++
Sbjct: 282 IVLSLAVNLGWGLWQMDVKNAFLQGELEDEVYMYPPPGLEHLVKRG--------NVLRLK 127
Query: 149 SASYSLPCP--------SSFLTSLGYAQSQSGHSLFT 177
A Y L S+ L G+ +S+ H+LFT
Sbjct: 126 KAIYGLKQSPRAWYNKLSTTLNGRGFRKSELDHTLFT 16
>BG452576 weakly similar to GP|12005223|gb|A reverse transcriptase-like
protein {Spiranthes hongkongensis}, partial (25%)
Length = 676
Score = 34.7 bits (78), Expect = 0.046
Identities = 16/43 (37%), Positives = 28/43 (64%)
Frame = +2
Query: 159 SFLTSLGYAQSQSGHSLFTKIHNQKNFTVILIFVDNLILPGND 201
S L SLGY QS + HS+F+ + FT+ L+++D+++ +D
Sbjct: 260 STLISLGYKQSPNDHSIFS---FGRRFTIFLVYLDDIVFARDD 379
>BE123913 weakly similar to GP|22093573|d polyprotein {Oryza sativa (japonica
cultivar-group)}, partial (8%)
Length = 503
Score = 31.2 bits (69), Expect = 0.51
Identities = 11/36 (30%), Positives = 23/36 (63%)
Frame = +1
Query: 165 GYAQSQSGHSLFTKIHNQKNFTVILIFVDNLILPGN 200
GY Q Q+ H++F K + +++++VD++ L G+
Sbjct: 52 GYIQCQTDHAMFIKHSSTVKKAILIVYVDDIFLTGD 159
>CB893680 weakly similar to GP|1167523|db ORF(AA 1-1338) {Nicotiana tabacum},
partial (7%)
Length = 780
Score = 30.0 bits (66), Expect = 1.1
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = -2
Query: 87 LFLLLAIATTQY*HLHQLDVNDTFIHGELDEKVYMVVPPFYS 128
+ LL+I + +L LDV F+ G+L E +YM P +S
Sbjct: 527 IMFLLSIVAIENLYLE*LDVKTAFLRGDLVEDIYMHQPEGFS 402
>BG647636
Length = 803
Score = 28.9 bits (63), Expect = 2.5
Identities = 17/43 (39%), Positives = 26/43 (59%)
Frame = +3
Query: 215 LCGSFLVLFVFQCANVNMAQIY*LKLVSWVLNGCLHPCLCLRT 257
+CGSFLVLF++ C ++ A+ K+ WVL P L L++
Sbjct: 579 VCGSFLVLFIW*C*AIHGAK----KIGWWVLKVV*FPHLILKS 695
>TC83658
Length = 834
Score = 28.5 bits (62), Expect = 3.3
Identities = 17/48 (35%), Positives = 25/48 (51%)
Frame = +3
Query: 229 NVNMAQIY*LKLVSWVLNGCLHPCLCLRTRLPLKSLLLWTLADVLVSS 276
NVN+ + L L+ W+ N CL+ C R L +LW+L + V S
Sbjct: 243 NVNIHHFFLLDLL-WIFNCCLNHQSCCRVGTRL*FFVLWSLLNPNVLS 383
>TC85814 similar to SP|P48631|FD62_SOYBN Omega-6 fatty acid desaturase
endoplasmic reticulum isozyme 2 (EC 1.14.99.-).
[Soybean], complete
Length = 1897
Score = 28.5 bits (62), Expect = 3.3
Identities = 13/46 (28%), Positives = 24/46 (51%), Gaps = 2/46 (4%)
Frame = -2
Query: 100 HLHQLDVNDTFIHGELDEKVYMVV--PPFYSHHALNQVYTDS*SLF 143
H+H+ + +IH E +Y+++ + + L Q+Y DS LF
Sbjct: 1689 HMHRFQIKVQYIHYYCSEMLYLILQSSKLFQYCLLLQIYQDSTKLF 1552
>TC82246 similar to PIR|T02580|T02580 hypothetical protein At2g39220
[imported] - Arabidopsis thaliana, partial (45%)
Length = 780
Score = 28.1 bits (61), Expect = 4.3
Identities = 15/43 (34%), Positives = 27/43 (61%), Gaps = 2/43 (4%)
Frame = -3
Query: 260 PLKSLLLWTLADVLVSSGS--FYI*LQYVLTYSLQHISLASTC 300
P+K L + L+++L S + + LQ+++ YS QH+ +A TC
Sbjct: 610 PIKFLKVTLLSNLLPSKQN*LHILLLQHLICYSHQHLYIAGTC 482
>BF640635
Length = 289
Score = 27.3 bits (59), Expect = 7.3
Identities = 16/50 (32%), Positives = 22/50 (44%)
Frame = +3
Query: 111 IHGELDEKVYMVVPPFYSHHALNQVYTDS*SLFMVSIRSASYSLPCPSSF 160
+ L VY+ PPF +A ++T ASY+ PCPS F
Sbjct: 171 LRNTLSVDVYLFSPPFSFSYA*ISLWT-----------CASYNAPCPSGF 287
>TC93570 similar to GP|21553436|gb|AAM62529.1 unknown {Arabidopsis
thaliana}, partial (57%)
Length = 759
Score = 27.3 bits (59), Expect = 7.3
Identities = 11/26 (42%), Positives = 19/26 (72%)
Frame = +1
Query: 151 SYSLPCPSSFLTSLGYAQSQSGHSLF 176
++SLPC ++L SLG+ QS+ +L+
Sbjct: 301 THSLPCIEAWLKSLGFNQSKDDRALW 378
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.351 0.154 0.540
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,270,636
Number of Sequences: 36976
Number of extensions: 185134
Number of successful extensions: 1740
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1739
length of query: 300
length of database: 9,014,727
effective HSP length: 96
effective length of query: 204
effective length of database: 5,465,031
effective search space: 1114866324
effective search space used: 1114866324
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (22.0 bits)
S2: 58 (26.9 bits)
Medicago: description of AC134049.15