Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC134049.15 + phase: 0 /pseudo
         (300 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BI309716 weakly similar to PIR|G96722|G9 hypothetical protein F2...    45  3e-05
CB893805 similar to GP|10177935|d copia-type polyprotein {Arabid...    33  7e-05
AW689768 weakly similar to GP|10177485|d polyprotein {Arabidopsi...    43  1e-04
BG587156 similar to PIR|G85055|G8 probable polyprotein [imported...    39  0.003
BG452576 weakly similar to GP|12005223|gb|A reverse transcriptas...    35  0.046
BE123913 weakly similar to GP|22093573|d polyprotein {Oryza sati...    31  0.51
CB893680 weakly similar to GP|1167523|db ORF(AA 1-1338) {Nicotia...    30  1.1
BG647636                                                               29  2.5
TC83658                                                                28  3.3
TC85814 similar to SP|P48631|FD62_SOYBN Omega-6 fatty acid desat...    28  3.3
TC82246 similar to PIR|T02580|T02580 hypothetical protein At2g39...    28  4.3
BF640635                                                               27  7.3
TC93570 similar to GP|21553436|gb|AAM62529.1 unknown {Arabidopsi...    27  7.3

>BI309716 weakly similar to PIR|G96722|G9 hypothetical protein F20P5.25
           [imported] - Arabidopsis thaliana, partial (10%)
          Length = 744

 Score = 45.1 bits (105), Expect = 3e-05
 Identities = 22/44 (50%), Positives = 30/44 (68%)
 Frame = +2

Query: 158 SSFLTSLGYAQSQSGHSLFTKIHNQKNFTVILIFVDNLILPGND 201
           S  L S GY QS S  SLFTK  +  +FT +L++VD+++L GND
Sbjct: 86  SESLISFGYLQSSSDFSLFTKFKDS-SFTTLLVYVDDIVLAGND 214


>CB893805 similar to GP|10177935|d copia-type polyprotein {Arabidopsis
           thaliana}, partial (14%)
          Length = 778

 Score = 32.7 bits (73), Expect(2) = 7e-05
 Identities = 14/43 (32%), Positives = 24/43 (55%)
 Frame = +3

Query: 159 SFLTSLGYAQSQSGHSLFTKIHNQKNFTVILIFVDNLILPGND 201
           ++ T  G+ +    H+LF K+       +I ++VD+LI  GND
Sbjct: 525 AYFTKEGFEKCPYEHTLFVKLSEGGKILIISLYVDDLIFIGND 653



 Score = 30.4 bits (67), Expect(2) = 7e-05
 Identities = 12/27 (44%), Positives = 20/27 (73%)
 Frame = +2

Query: 101 LHQLDVNDTFIHGELDEKVYMVVPPFY 127
           ++QLDV   F++GEL+E+V++  P  Y
Sbjct: 359 VYQLDVKSAFLYGELNEEVFVDQPQGY 439


>AW689768 weakly similar to GP|10177485|d polyprotein {Arabidopsis thaliana},
           partial (9%)
          Length = 675

 Score = 43.1 bits (100), Expect = 1e-04
 Identities = 36/122 (29%), Positives = 59/122 (47%), Gaps = 10/122 (8%)
 Frame = +1

Query: 89  LLLAIATTQY*HLHQLDVNDTFIHGELDEKVYMVVPPFYSHHALNQVYTDS*SLFMVSIR 148
           L+L IA T    + Q+D+N+ F++G L E+VYM  P         Q +  +    +  + 
Sbjct: 337 LILTIAITYKWEIQQIDINNAFLNGFLQEEVYMSQP---------QGFEAANKSLVCKLN 489

Query: 149 SASYSL-PCPSSFLTSLGYAQSQSGHSLFTK--------IHNQKNFTVIL-IFVDNLILP 198
            + Y L   P ++   L  AQ Q G   FTK        I+NQ    + L I+VD++++ 
Sbjct: 490 KSLYGLKQAPRAWYEXLTSAQIQFG---FTKSRCDPSLLIYNQNGACIYLXIYVDDILIT 660

Query: 199 GN 200
           G+
Sbjct: 661 GS 666


>BG587156 similar to PIR|G85055|G8 probable polyprotein [imported] -
           Arabidopsis thaliana, partial (17%)
          Length = 618

 Score = 38.5 bits (88), Expect = 0.003
 Identities = 27/97 (27%), Positives = 46/97 (46%), Gaps = 8/97 (8%)
 Frame = -1

Query: 89  LLLAIATTQY*HLHQLDVNDTFIHGELDEKVYMVVPPFYSHHALNQVYTDS*SLFMVSIR 148
           ++L++A      L Q+DV + F+ GEL+++VYM  PP   H              ++ ++
Sbjct: 282 IVLSLAVNLGWGLWQMDVKNAFLQGELEDEVYMYPPPGLEHLVKRG--------NVLRLK 127

Query: 149 SASYSLPCP--------SSFLTSLGYAQSQSGHSLFT 177
            A Y L           S+ L   G+ +S+  H+LFT
Sbjct: 126 KAIYGLKQSPRAWYNKLSTTLNGRGFRKSELDHTLFT 16


>BG452576 weakly similar to GP|12005223|gb|A reverse transcriptase-like
           protein {Spiranthes hongkongensis}, partial (25%)
          Length = 676

 Score = 34.7 bits (78), Expect = 0.046
 Identities = 16/43 (37%), Positives = 28/43 (64%)
 Frame = +2

Query: 159 SFLTSLGYAQSQSGHSLFTKIHNQKNFTVILIFVDNLILPGND 201
           S L SLGY QS + HS+F+     + FT+ L+++D+++   +D
Sbjct: 260 STLISLGYKQSPNDHSIFS---FGRRFTIFLVYLDDIVFARDD 379


>BE123913 weakly similar to GP|22093573|d polyprotein {Oryza sativa (japonica
           cultivar-group)}, partial (8%)
          Length = 503

 Score = 31.2 bits (69), Expect = 0.51
 Identities = 11/36 (30%), Positives = 23/36 (63%)
 Frame = +1

Query: 165 GYAQSQSGHSLFTKIHNQKNFTVILIFVDNLILPGN 200
           GY Q Q+ H++F K  +     +++++VD++ L G+
Sbjct: 52  GYIQCQTDHAMFIKHSSTVKKAILIVYVDDIFLTGD 159


>CB893680 weakly similar to GP|1167523|db ORF(AA 1-1338) {Nicotiana tabacum},
           partial (7%)
          Length = 780

 Score = 30.0 bits (66), Expect = 1.1
 Identities = 15/42 (35%), Positives = 23/42 (54%)
 Frame = -2

Query: 87  LFLLLAIATTQY*HLHQLDVNDTFIHGELDEKVYMVVPPFYS 128
           +  LL+I   +  +L  LDV   F+ G+L E +YM  P  +S
Sbjct: 527 IMFLLSIVAIENLYLE*LDVKTAFLRGDLVEDIYMHQPEGFS 402


>BG647636 
          Length = 803

 Score = 28.9 bits (63), Expect = 2.5
 Identities = 17/43 (39%), Positives = 26/43 (59%)
 Frame = +3

Query: 215 LCGSFLVLFVFQCANVNMAQIY*LKLVSWVLNGCLHPCLCLRT 257
           +CGSFLVLF++ C  ++ A+    K+  WVL     P L L++
Sbjct: 579 VCGSFLVLFIW*C*AIHGAK----KIGWWVLKVV*FPHLILKS 695


>TC83658 
          Length = 834

 Score = 28.5 bits (62), Expect = 3.3
 Identities = 17/48 (35%), Positives = 25/48 (51%)
 Frame = +3

Query: 229 NVNMAQIY*LKLVSWVLNGCLHPCLCLRTRLPLKSLLLWTLADVLVSS 276
           NVN+   + L L+ W+ N CL+   C R    L   +LW+L +  V S
Sbjct: 243 NVNIHHFFLLDLL-WIFNCCLNHQSCCRVGTRL*FFVLWSLLNPNVLS 383


>TC85814 similar to SP|P48631|FD62_SOYBN Omega-6 fatty acid desaturase
            endoplasmic reticulum isozyme 2 (EC 1.14.99.-).
            [Soybean], complete
          Length = 1897

 Score = 28.5 bits (62), Expect = 3.3
 Identities = 13/46 (28%), Positives = 24/46 (51%), Gaps = 2/46 (4%)
 Frame = -2

Query: 100  HLHQLDVNDTFIHGELDEKVYMVV--PPFYSHHALNQVYTDS*SLF 143
            H+H+  +   +IH    E +Y+++     + +  L Q+Y DS  LF
Sbjct: 1689 HMHRFQIKVQYIHYYCSEMLYLILQSSKLFQYCLLLQIYQDSTKLF 1552


>TC82246 similar to PIR|T02580|T02580 hypothetical protein At2g39220
           [imported] - Arabidopsis thaliana, partial (45%)
          Length = 780

 Score = 28.1 bits (61), Expect = 4.3
 Identities = 15/43 (34%), Positives = 27/43 (61%), Gaps = 2/43 (4%)
 Frame = -3

Query: 260 PLKSLLLWTLADVLVSSGS--FYI*LQYVLTYSLQHISLASTC 300
           P+K L +  L+++L S  +    + LQ+++ YS QH+ +A TC
Sbjct: 610 PIKFLKVTLLSNLLPSKQN*LHILLLQHLICYSHQHLYIAGTC 482


>BF640635 
          Length = 289

 Score = 27.3 bits (59), Expect = 7.3
 Identities = 16/50 (32%), Positives = 22/50 (44%)
 Frame = +3

Query: 111 IHGELDEKVYMVVPPFYSHHALNQVYTDS*SLFMVSIRSASYSLPCPSSF 160
           +   L   VY+  PPF   +A   ++T            ASY+ PCPS F
Sbjct: 171 LRNTLSVDVYLFSPPFSFSYA*ISLWT-----------CASYNAPCPSGF 287


>TC93570 similar to GP|21553436|gb|AAM62529.1 unknown {Arabidopsis
           thaliana}, partial (57%)
          Length = 759

 Score = 27.3 bits (59), Expect = 7.3
 Identities = 11/26 (42%), Positives = 19/26 (72%)
 Frame = +1

Query: 151 SYSLPCPSSFLTSLGYAQSQSGHSLF 176
           ++SLPC  ++L SLG+ QS+   +L+
Sbjct: 301 THSLPCIEAWLKSLGFNQSKDDRALW 378


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.351    0.154    0.540 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,270,636
Number of Sequences: 36976
Number of extensions: 185134
Number of successful extensions: 1740
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1739
length of query: 300
length of database: 9,014,727
effective HSP length: 96
effective length of query: 204
effective length of database: 5,465,031
effective search space: 1114866324
effective search space used: 1114866324
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (22.0 bits)
S2: 58 (26.9 bits)


Medicago: description of AC134049.15