Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC133139.2 + phase: 0 /pseudo
         (155 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

CB891859 similar to GP|6175165|gb| Mutator-like transposase {Ara...    49  1e-06
TC79804 weakly similar to GP|6175165|gb|AAF04891.1| Mutator-like...    46  5e-06
AL375901 similar to GP|9759134|dbj mutator-like transposase-like...    45  2e-05
TC90326 similar to GP|13775111|gb|AAK39132.1 bZIP transcription ...    32  0.079
TC86762 similar to SP|P54967|BIOB_ARATH Biotin synthase (EC 2.8....    32  0.10
TC86954 similar to GP|11385433|gb|AAG34799.1 glutathione S-trans...    29  0.67
BG451959 homologue to GP|6175165|gb| Mutator-like transposase {A...    27  2.5
BF646436 weakly similar to GP|9828634|gb F1N21.5 {Arabidopsis th...    26  5.6
TC92824 similar to PIR|H86263|H86263 hypothetical protein AAD310...    26  5.6
TC76323 homologue to GP|12054507|emb|CAC20221. ribosomal protein...    26  5.6
TC81348 similar to GP|17979464|gb|AAL50069.1 At1g68530/T26J14_10...    26  7.4
TC79878 similar to GP|15485612|emb|CAC67408. insulin degrading e...    26  7.4
CA917564                                                               26  7.4
TC87218 similar to GP|8778643|gb|AAF79651.1| F5O11.25 {Arabidops...    25  9.6
TC89125 similar to PIR|H86253|H86253 hypothetical protein [impor...    25  9.6
TC88417 similar to SP|Q40285|UFO2_MANES Flavonol 3-O-glucosyltra...    25  9.6
TC86955 similar to GP|11385433|gb|AAG34799.1 glutathione S-trans...    25  9.6
CA920463 weakly similar to GP|2852684|gb|A resistance protein ca...    25  9.6
BF519771 homologue to GP|7297246|gb| CG5177-PA {Drosophila melan...    25  9.6

>CB891859 similar to GP|6175165|gb| Mutator-like transposase {Arabidopsis
           thaliana}, partial (27%)
          Length = 625

 Score = 48.5 bits (114), Expect = 1e-06
 Identities = 34/121 (28%), Positives = 53/121 (43%), Gaps = 1/121 (0%)
 Frame = +1

Query: 15  PDLGLISDRHESIKSVVRRLDSNWHHVFCIRHITQLFEDFQEHRDEEKSKKYGLNNINFL 74
           P L ++SDR + I   V        H FC+RH++  F      R E  +       +N L
Sbjct: 28  PRLTILSDRQQGIVDGVEANFPTAFHGFCMRHLSDSF------RKEFNNTML----VNLL 177

Query: 75  -PSGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFN 133
             +   +T         +I + S D   W+   P + W  A+ +G R+GH+T NI E  N
Sbjct: 178 WEAANCLTIIEFEGKVMEIEEISQDAAYWIRRVPPRLWATAYFEGHRFGHLTANIVEALN 357

Query: 134 N 134
           +
Sbjct: 358 S 360


>TC79804 weakly similar to GP|6175165|gb|AAF04891.1| Mutator-like transposase
            {Arabidopsis thaliana}, partial (57%)
          Length = 2129

 Score = 46.2 bits (108), Expect = 5e-06
 Identities = 30/118 (25%), Positives = 55/118 (46%), Gaps = 2/118 (1%)
 Frame = +1

Query: 15   PDLGLISDRHESIKSVVRRLDSNWHHVFCIRHITQ-LFEDFQEHRDEEKSKKYGLNNINF 73
            P +  +SD  + I   +RR      H FC+RH+++ + ++F+  R            I+ 
Sbjct: 946  PQIIFLSDGQKGIVDAIRRKFPRSSHAFCMRHLSENIGKEFKNSR-----------LIHL 1092

Query: 74   LPSG-YSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISE 130
            L S  Y+ T  +      +I + S +   W+      QW   + +G R+GH+++NI E
Sbjct: 1093 LWSAAYATTINAFREKMAEIEEVSPNASMWLQHFHPSQWALVYFEGTRYGHLSSNIEE 1266


>AL375901 similar to GP|9759134|dbj mutator-like transposase-like protein
           {Arabidopsis thaliana}, partial (27%)
          Length = 493

 Score = 44.7 bits (104), Expect = 2e-05
 Identities = 29/95 (30%), Positives = 46/95 (47%)
 Frame = +3

Query: 40  HVFCIRHITQLFEDFQEHRDEEKSKKYGLNNINFLPSGYSMTQPSITHYRNQIRDWSDDV 99
           H FC+R++++ F      RD  K+ K  L NI F  + Y++T         ++ + S DV
Sbjct: 24  HGFCLRYVSENF------RDTFKNTK--LVNI-FWNAVYALTAAEFESKITEMIEVSQDV 176

Query: 100 VKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNN 134
           + W    P   W  A+  G R+GH T  ++E   N
Sbjct: 177 ISWFQHFPPFLWAVAYFDGVRYGHFTLGVTELLYN 281


>TC90326 similar to GP|13775111|gb|AAK39132.1 bZIP transcription factor 6
           {Phaseolus vulgaris}, partial (34%)
          Length = 872

 Score = 32.3 bits (72), Expect = 0.079
 Identities = 16/57 (28%), Positives = 32/57 (56%)
 Frame = -2

Query: 36  SNWHHVFCIRHITQLFEDFQEHRDEEKSKKYGLNNINFLPSGYSMTQPSITHYRNQI 92
           SN+ H+  ++  TQ +  FQ ++D+EK   +GL +        S+T P++T   +++
Sbjct: 709 SNFIHLLSLKSQTQNYHYFQHYQDQEKHHSFGLAS--------SLTTPALTKNSSKV 563


>TC86762 similar to SP|P54967|BIOB_ARATH Biotin synthase (EC 2.8.1.6)
           (Biotin synthetase). [Mouse-ear cress] {Arabidopsis
           thaliana}, partial (87%)
          Length = 1555

 Score = 32.0 bits (71), Expect = 0.10
 Identities = 25/93 (26%), Positives = 38/93 (39%), Gaps = 1/93 (1%)
 Frame = +1

Query: 58  RDEEKSKKYGLNNIN-FLPSGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHD 116
           R + +S  + L + N F  S  +  Q   T       DW+ D VK + D+P    L    
Sbjct: 289 RSQSRSSIWVLQHCNSFSTSSAAAIQAEKTIQNGPRNDWTKDEVKSIYDSPILDLLFHGA 468

Query: 117 QGRRWGHITTNISECFNNVLKGGPQSSNHTNCP 149
           Q  R  H    + +C    +K G  S + + CP
Sbjct: 469 QVHRHAHNFREVQQCTLLSVKTGGCSEDCSYCP 567


>TC86954 similar to GP|11385433|gb|AAG34799.1 glutathione S-transferase GST
           9 {Glycine max}, partial (98%)
          Length = 664

 Score = 29.3 bits (64), Expect = 0.67
 Identities = 15/41 (36%), Positives = 23/41 (55%), Gaps = 3/41 (7%)
 Frame = +3

Query: 83  PSITHYRNQIRDWSDDVVKWVDDNPKKQWL---QAHDQGRR 120
           PS  + R Q R W D + K V D  +K WL   + H++G++
Sbjct: 309 PSDPYERAQARFWVDYIDKKVYDTWRKMWLSKGEEHEEGKK 431


>BG451959 homologue to GP|6175165|gb| Mutator-like transposase {Arabidopsis
           thaliana}, partial (28%)
          Length = 658

 Score = 27.3 bits (59), Expect = 2.5
 Identities = 13/37 (35%), Positives = 19/37 (51%)
 Frame = +2

Query: 15  PDLGLISDRHESIKSVVRRLDSNWHHVFCIRHITQLF 51
           P L ++SDR + I   V        H FC+RH++  F
Sbjct: 539 PRLTILSDRQQGIVDGVEANFPTAFHGFCMRHLSDNF 649


>BF646436 weakly similar to GP|9828634|gb F1N21.5 {Arabidopsis thaliana},
           partial (11%)
          Length = 659

 Score = 26.2 bits (56), Expect = 5.6
 Identities = 16/55 (29%), Positives = 26/55 (47%)
 Frame = +1

Query: 54  FQEHRDEEKSKKYGLNNINFLPSGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPK 108
           F+  R  E+ ++  LNNINFL    +     + H R+++     D     D+N K
Sbjct: 466 FESRRLFEEKRESELNNINFLREVANRGMEEMKHQRSKLEREKQD----ADENRK 618


>TC92824 similar to PIR|H86263|H86263 hypothetical protein AAD31053.1
           [imported] - Arabidopsis thaliana, partial (48%)
          Length = 578

 Score = 26.2 bits (56), Expect = 5.6
 Identities = 8/15 (53%), Positives = 11/15 (73%)
 Frame = +2

Query: 35  DSNWHHVFCIRHITQ 49
           D+ WH  FC RHI++
Sbjct: 404 DAGWHCSFCFRHISE 448


>TC76323 homologue to GP|12054507|emb|CAC20221. ribosomal protein L2
           {Glycine max}, complete
          Length = 1031

 Score = 26.2 bits (56), Expect = 5.6
 Identities = 19/68 (27%), Positives = 34/68 (49%), Gaps = 1/68 (1%)
 Frame = -1

Query: 20  ISDRHESIKSVV-RRLDSNWHHVFCIRHITQLFEDFQEHRDEEKSKKYGLNNINFLPSGY 78
           + DR   I + V ++L + W   F  +H          ++  +++ +Y LNN+N L  G+
Sbjct: 977 LQDRCNKI*NAV*QKLHNFWGKWFSKQH-----NSLTRNKIGKQAIEYNLNNLNCLFIGF 813

Query: 79  SMTQPSIT 86
           S T  S+T
Sbjct: 812 SSTCSSLT 789


>TC81348 similar to GP|17979464|gb|AAL50069.1 At1g68530/T26J14_10
           {Arabidopsis thaliana}, partial (53%)
          Length = 906

 Score = 25.8 bits (55), Expect = 7.4
 Identities = 8/20 (40%), Positives = 12/20 (60%)
 Frame = -3

Query: 32  RRLDSNWHHVFCIRHITQLF 51
           R +  +W HVFC+R +   F
Sbjct: 106 REIRQDWRHVFCLRKVLFFF 47


>TC79878 similar to GP|15485612|emb|CAC67408. insulin degrading enzyme
           {Lycopersicon esculentum}, partial (32%)
          Length = 1141

 Score = 25.8 bits (55), Expect = 7.4
 Identities = 7/29 (24%), Positives = 15/29 (51%)
 Frame = -1

Query: 38  WHHVFCIRHITQLFEDFQEHRDEEKSKKY 66
           WH   C     ++ +D Q+HR  ++ + +
Sbjct: 370 WHKTACALENERVLQDHQDHRSNQRQRSW 284


>CA917564 
          Length = 656

 Score = 25.8 bits (55), Expect = 7.4
 Identities = 10/31 (32%), Positives = 17/31 (54%)
 Frame = -3

Query: 121 WGHITTNISECFNNVLKGGPQSSNHTNCPGN 151
           +G +   + +C N +LKG P+  N   C G+
Sbjct: 135 FGALI*TLGDCNNGLLKGSPEIVNECCCRGS 43


>TC87218 similar to GP|8778643|gb|AAF79651.1| F5O11.25 {Arabidopsis
           thaliana}, partial (85%)
          Length = 1557

 Score = 25.4 bits (54), Expect = 9.6
 Identities = 24/85 (28%), Positives = 35/85 (40%), Gaps = 16/85 (18%)
 Frame = +3

Query: 59  DEEKSKKYGLNNINFLPSGYSMT----------------QPSITHYRNQIRDWSDDVVKW 102
           D+ K+  Y + N NF    + +T                QP ITH R++ R  +      
Sbjct: 24  DQTKTICYYIYNNNFFHISFLLTFSCNGGSTNMDNKKNEQPKITHIRHK-RPITIRHTTN 200

Query: 103 VDDNPKKQWLQAHDQGRRWGHITTN 127
               PKKQW+Q H Q +   + T N
Sbjct: 201 TTK*PKKQWIQ-HQQQQPSNNFTYN 272


>TC89125 similar to PIR|H86253|H86253 hypothetical protein [imported] -
            Arabidopsis thaliana, partial (70%)
          Length = 1334

 Score = 25.4 bits (54), Expect = 9.6
 Identities = 11/24 (45%), Positives = 13/24 (53%)
 Frame = +3

Query: 63   SKKYGLNNINFLPSGYSMTQPSIT 86
            SK Y  N   F+PSGY    P+ T
Sbjct: 996  SKDYFSNGAYFIPSGYGSCAPNYT 1067


>TC88417 similar to SP|Q40285|UFO2_MANES Flavonol 3-O-glucosyltransferase 2
           (EC 2.4.1.91) (UDP-glucose flavonoid
           3-O-glucosyltransferase 2), partial (47%)
          Length = 828

 Score = 25.4 bits (54), Expect = 9.6
 Identities = 7/11 (63%), Positives = 10/11 (90%)
 Frame = +3

Query: 97  DDVVKWVDDNP 107
           DD++KW+DD P
Sbjct: 27  DDIIKWLDDQP 59


>TC86955 similar to GP|11385433|gb|AAG34799.1 glutathione S-transferase GST
           9 {Glycine max}, complete
          Length = 1065

 Score = 25.4 bits (54), Expect = 9.6
 Identities = 14/35 (40%), Positives = 17/35 (48%)
 Frame = +1

Query: 83  PSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQ 117
           PS  + R Q R W D   K V D  KK WL   ++
Sbjct: 391 PSDPYERAQARFWVDYSDKKVYDTWKKMWLSKGEE 495


>CA920463 weakly similar to GP|2852684|gb|A resistance protein candidate
           {Lactuca sativa}, partial (6%)
          Length = 810

 Score = 25.4 bits (54), Expect = 9.6
 Identities = 11/26 (42%), Positives = 16/26 (61%)
 Frame = -1

Query: 95  WSDDVVKWVDDNPKKQWLQAHDQGRR 120
           WS+D VKW++    K  LQ  D+G +
Sbjct: 726 WSEDRVKWIE---VKNLLQVGDEGSK 658


>BF519771 homologue to GP|7297246|gb| CG5177-PA {Drosophila melanogaster},
           partial (61%)
          Length = 593

 Score = 25.4 bits (54), Expect = 9.6
 Identities = 9/22 (40%), Positives = 15/22 (67%)
 Frame = -2

Query: 67  GLNNINFLPSGYSMTQPSITHY 88
           G++ +NF P GYS ++P +  Y
Sbjct: 418 GISILNFFPDGYSTSKP*LPAY 353


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.318    0.135    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,640,592
Number of Sequences: 36976
Number of extensions: 102316
Number of successful extensions: 449
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 448
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 448
length of query: 155
length of database: 9,014,727
effective HSP length: 88
effective length of query: 67
effective length of database: 5,760,839
effective search space: 385976213
effective search space used: 385976213
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)


Medicago: description of AC133139.2