BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC131248.8 + phase: 0
(285 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC88121 homologue to PIR|T06558|T06558 choline-phosphate cytidyl... 586 e-168
TC88230 similar to PIR|T07981|T07981 probable choline-phosphate ... 355 1e-98
TC77924 similar to PIR|H84807|H84807 probable phospholipid cytid... 110 8e-25
TC88702 similar to GP|13129435|gb|AAK13093.1 Putative phospholip... 92 3e-19
TC92238 weakly similar to GP|14456138|emb|CAC41653. hypothetical... 43 1e-04
AW695732 similar to GP|13129435|gb| Putative phospholipid cytidy... 38 0.004
CB065364 homologue to PIR|A83022|A83 LPS biosynthesis protein Rf... 30 1.1
AW774294 30 1.4
TC80044 weakly similar to GP|7300238|gb|AAF55402.1| CG4090 gene ... 29 1.8
BQ144511 similar to GP|18855061|gb| putative RNA helicase {Oryza... 28 3.1
TC85121 similar to GP|12855997|dbj|BAB30531. evidence:NAS~putati... 28 3.1
BQ150646 28 3.1
TC93418 28 3.1
TC89807 weakly similar to PIR|B96600|B96600 protein F14J16.14 [i... 28 3.1
TC86742 similar to PIR|T01541|T01541 hypothetical protein A_IG00... 28 3.1
TC92129 homologue to PIR|H84807|H84807 probable phospholipid cyt... 28 4.0
TC80424 homologue to GP|21593052|gb|AAM65001.1 DAG protein puta... 27 6.9
TC84306 weakly similar to PIR|T01047|T01047 hypothetical protein... 27 6.9
BG451797 similar to GP|18028626|gb| maturase {Zippelia begoniifo... 27 6.9
CA918758 weakly similar to PIR|S74637|S746 lysine decarboxylase ... 27 9.0
>TC88121 homologue to PIR|T06558|T06558 choline-phosphate
cytidylyltransferase (EC 2.7.7.15) - garden pea, partial
(97%)
Length = 1063
Score = 586 bits (1511), Expect = e-168
Identities = 285/285 (100%), Positives = 285/285 (100%)
Frame = +3
Query: 1 MNEQDDSEVGKNDKPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCSDEITHKY 60
MNEQDDSEVGKNDKPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCSDEITHKY
Sbjct: 96 MNEQDDSEVGKNDKPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCSDEITHKY 275
Query: 61 KGKTVMNEQERYESLRHCKWVDEVIPDVPWVINQEFIDKHKIDYVAHDALPYADTSGAGN 120
KGKTVMNEQERYESLRHCKWVDEVIPDVPWVINQEFIDKHKIDYVAHDALPYADTSGAGN
Sbjct: 276 KGKTVMNEQERYESLRHCKWVDEVIPDVPWVINQEFIDKHKIDYVAHDALPYADTSGAGN 455
Query: 121 DVYEFVKAIGKFKETKRTEGISTSDIIMRIIKDYNQYVMRNLDRGYSRKELGVSYVKEKR 180
DVYEFVKAIGKFKETKRTEGISTSDIIMRIIKDYNQYVMRNLDRGYSRKELGVSYVKEKR
Sbjct: 456 DVYEFVKAIGKFKETKRTEGISTSDIIMRIIKDYNQYVMRNLDRGYSRKELGVSYVKEKR 635
Query: 181 LRMNMGLKKLQERVKKQQETVGKKIGTVRRIAGMNRTEWVENADRLVAGFLEMFEEGCHK 240
LRMNMGLKKLQERVKKQQETVGKKIGTVRRIAGMNRTEWVENADRLVAGFLEMFEEGCHK
Sbjct: 636 LRMNMGLKKLQERVKKQQETVGKKIGTVRRIAGMNRTEWVENADRLVAGFLEMFEEGCHK 815
Query: 241 MGTAIRDRIQEQLKAQQLKSLLYDEWDDDVDDEFYEDESVEYYSD 285
MGTAIRDRIQEQLKAQQLKSLLYDEWDDDVDDEFYEDESVEYYSD
Sbjct: 816 MGTAIRDRIQEQLKAQQLKSLLYDEWDDDVDDEFYEDESVEYYSD 950
>TC88230 similar to PIR|T07981|T07981 probable choline-phosphate
cytidylyltransferase (EC 2.7.7.15) (clone CCT1) - rape,
partial (59%)
Length = 1364
Score = 355 bits (910), Expect = 1e-98
Identities = 166/192 (86%), Positives = 182/192 (94%)
Frame = +3
Query: 13 DKPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCSDEITHKYKGKTVMNEQERY 72
D+PVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCSD +THKYKGKTVM E ERY
Sbjct: 111 DRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCSDAVTHKYKGKTVMTEDERY 290
Query: 73 ESLRHCKWVDEVIPDVPWVINQEFIDKHKIDYVAHDALPYADTSGAGNDVYEFVKAIGKF 132
ESLRHCKWVDEVIPD PWVINQEF+DK+KID+VAHD+LPYADTSGA NDVYEFVKA+G+F
Sbjct: 291 ESLRHCKWVDEVIPDAPWVINQEFLDKNKIDFVAHDSLPYADTSGAANDVYEFVKAVGRF 470
Query: 133 KETKRTEGISTSDIIMRIIKDYNQYVMRNLDRGYSRKELGVSYVKEKRLRMNMGLKKLQE 192
KET+RTEGISTSDIIMRI+KDYNQYV+RNLDRGYSRK+LGVSYVKEKRLR+N LK LQE
Sbjct: 471 KETQRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKDLGVSYVKEKRLRVNRRLKTLQE 650
Query: 193 RVKKQQETVGKK 204
+V++QQE VG K
Sbjct: 651 KVREQQEKVGVK 686
>TC77924 similar to PIR|H84807|H84807 probable phospholipid
cytidylyltransferase [imported] - Arabidopsis thaliana,
partial (86%)
Length = 1691
Score = 110 bits (274), Expect = 8e-25
Identities = 58/145 (40%), Positives = 84/145 (57%), Gaps = 4/145 (2%)
Frame = +2
Query: 11 KNDKPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCSDEITHKYKGKTVMNEQE 70
+N KP+RVY DG +D+ H+GH +L QA+ L+VG SD+ KG V E
Sbjct: 215 RNKKPIRVYMDGCFDMMHYGHCNALRQARAL--GDQLIVGVVSDDEIIANKGPPVTPLHE 388
Query: 71 RYESLRHCKWVDEVIPDVPWVINQEFI----DKHKIDYVAHDALPYADTSGAGNDVYEFV 126
R + KWVDEVIP+ P+ I +EF+ D++ IDY+ H P G D Y
Sbjct: 389 RLIMVNAVKWVDEVIPEAPYAITEEFMKKLFDEYNIDYIIHGDDPCVLPD--GTDAYAHA 562
Query: 127 KAIGKFKETKRTEGISTSDIIMRII 151
K G++K+ KRTEG+S++DI+ R++
Sbjct: 563 KKAGRYKQIKRTEGVSSTDIVGRML 637
Score = 78.6 bits (192), Expect = 3e-15
Identities = 50/156 (32%), Positives = 78/156 (49%), Gaps = 3/156 (1%)
Frame = +2
Query: 18 VYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCSDEITHKYKG--KTVMNEQERYESL 75
VY DG +DLFH GH L A+ +LLVG +D+ +G + +M+ ER S+
Sbjct: 839 VYIDGAFDLFHAGHVEILRLARDR--GDFLLVGIHTDQTVSATRGLHRPIMSLHERSLSV 1012
Query: 76 RHCKWVDEVIPDVPWVINQEFIDKHKIDYVAH-DALPYADTSGAGNDVYEFVKAIGKFKE 134
C++VDEVI PW ++++ I I V H D ++ Y ++G F+
Sbjct: 1013LACRYVDEVIIGAPWEVSKDMITTFNISLVVHGTTAENTDFQKEQSNPYAVPISLGIFQI 1192
Query: 135 TKRTEGISTSDIIMRIIKDYNQYVMRNLDRGYSRKE 170
I+T+ II RI+ ++ Y RN +G S K+
Sbjct: 1193LDSPLDITTTTIIRRIVSNHEAYQKRNEKKGESEKK 1300
>TC88702 similar to GP|13129435|gb|AAK13093.1 Putative phospholipid
cytidylyltransferase {Oryza sativa}, partial (17%)
Length = 801
Score = 91.7 bits (226), Expect = 3e-19
Identities = 54/128 (42%), Positives = 70/128 (54%), Gaps = 4/128 (3%)
Frame = +1
Query: 16 VRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCSDEITHKYKGKTVMNEQERYESL 75
VRVY DG +DL H+GHA +L QAK L+VG SDE KG V++ ER +
Sbjct: 430 VRVYMDGCFDLMHYGHANALRQAKAL--GDELVVGLVSDEEIVANKGPPVLSMDERLALV 603
Query: 76 RHCKWVDEVIPDVPWVINQEFIDK----HKIDYVAHDALPYADTSGAGNDVYEFVKAIGK 131
KWVDEVI D P+ I + F+++ + IDYV H P G D Y K G+
Sbjct: 604 SGLKWVDEVITDAPYAITETFLNRLFHEYNIDYVIHGDDPCLLPD--GTDAYAAAKKAGR 777
Query: 132 FKETKRTE 139
+K+ KRTE
Sbjct: 778 YKQIKRTE 801
>TC92238 weakly similar to GP|14456138|emb|CAC41653. hypothetical protein
{Ustilago maydis}, partial (5%)
Length = 641
Score = 43.1 bits (100), Expect = 1e-04
Identities = 25/54 (46%), Positives = 31/54 (57%), Gaps = 6/54 (11%)
Frame = -3
Query: 230 FLEMFEEGCHKMG----TAIRDRIQEQLKAQQL--KSLLYDEWDDDVDDEFYED 277
F F GCH MG TAI DRIQE L+ QQ +S+ DD D+E+Y+D
Sbjct: 591 FKLFFSVGCHCMGIYQGTAICDRIQESLRGQQPNDESIFLQNGKDDEDEEYYDD 430
>AW695732 similar to GP|13129435|gb| Putative phospholipid
cytidylyltransferase {Oryza sativa}, partial (7%)
Length = 497
Score = 38.1 bits (87), Expect = 0.004
Identities = 15/24 (62%), Positives = 19/24 (78%)
Frame = +3
Query: 16 VRVYADGIYDLFHFGHARSLEQAK 39
V VY DG +DL H+GHA +L+QAK
Sbjct: 330 VHVYMDGCFDLMHYGHANALKQAK 401
>CB065364 homologue to PIR|A83022|A83 LPS biosynthesis protein RfaE PA4996
[imported] - Pseudomonas aeruginosa (strain PAO1),
partial (47%)
Length = 682
Score = 30.0 bits (66), Expect = 1.1
Identities = 21/76 (27%), Positives = 31/76 (40%)
Frame = +2
Query: 18 VYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCSDEITHKYKGKTVMNEQERYESLRH 77
V+ +G +D+ H GH LEQA+ + V + K G+ + + R L
Sbjct: 452 VFTNGCFDILHAGHVTYLEQARAQGDRLIVAVNDDASVSRLKGPGRPINSVDRRMAVLAG 631
Query: 78 CKWVDEVIPDVPWVIN 93
VD WVIN
Sbjct: 632 LGAVD-------WVIN 658
>AW774294
Length = 690
Score = 29.6 bits (65), Expect = 1.4
Identities = 17/48 (35%), Positives = 28/48 (57%)
Frame = -3
Query: 158 VMRNLDRGYSRKELGVSYVKEKRLRMNMGLKKLQERVKKQQETVGKKI 205
+ + ++ G+++K +S KEK LKK Q+R+K Q+T KKI
Sbjct: 670 INKKMNEGFTKKFEKISQTKEK-------LKKKQKRLKINQKTKTKKI 548
>TC80044 weakly similar to GP|7300238|gb|AAF55402.1| CG4090 gene product
{Drosophila melanogaster}, partial (2%)
Length = 1225
Score = 29.3 bits (64), Expect = 1.8
Identities = 23/68 (33%), Positives = 35/68 (50%), Gaps = 1/68 (1%)
Frame = -2
Query: 212 AGMNRTEWVENADRLVAGFLEMFEEGCHKMGTAIRDRIQEQLKAQQLKSLLYDEW-DDDV 270
A +N ++ V N D + E F+E D E L+ + L+ L DE+ +DD+
Sbjct: 438 AAINFSKQVINEDVVHDDIDEEFQE---------EDVDDEFLEDEFLEDNLSDEFQEDDI 286
Query: 271 DDEFYEDE 278
DDEF ED+
Sbjct: 285 DDEFQEDD 262
>BQ144511 similar to GP|18855061|gb| putative RNA helicase {Oryza sativa},
partial (3%)
Length = 1356
Score = 28.5 bits (62), Expect = 3.1
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = +3
Query: 204 KIGTVRRIAGMNRTEWVENADRLVAGFLEMFEEGCHKMGTAIRDRIQ 250
++GT RR T W E A R V ++ ++G + GT +RD Q
Sbjct: 1164 RVGTYRRDRAATLTAWREEAVRAVRACYDINKDGNRESGT-LRDETQ 1301
>TC85121 similar to GP|12855997|dbj|BAB30531.
evidence:NAS~putative~unclassifiable {Mus musculus},
partial (10%)
Length = 482
Score = 28.5 bits (62), Expect = 3.1
Identities = 13/35 (37%), Positives = 21/35 (59%)
Frame = +2
Query: 251 EQLKAQQLKSLLYDEWDDDVDDEFYEDESVEYYSD 285
+ + A + +++ DD+ DDE YEDE E Y+D
Sbjct: 86 DPMDAALISMMMHSHMDDEEDDEDYEDE--EEYTD 184
>BQ150646
Length = 917
Score = 28.5 bits (62), Expect = 3.1
Identities = 13/32 (40%), Positives = 16/32 (49%)
Frame = +2
Query: 15 PVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 46
P+ V + DLFHF +A SL S P Y
Sbjct: 302 PILV*PPTLIDLFHFSYADSLSDCVSSLPTQY 397
>TC93418
Length = 533
Score = 28.5 bits (62), Expect = 3.1
Identities = 10/38 (26%), Positives = 20/38 (52%)
Frame = +3
Query: 247 DRIQEQLKAQQLKSLLYDEWDDDVDDEFYEDESVEYYS 284
D Q +L++ EW + +DD +++ S+E+ S
Sbjct: 216 DHYDNQSALHVTSNLIFHEWTNHIDDHHFQNISIEFVS 329
>TC89807 weakly similar to PIR|B96600|B96600 protein F14J16.14 [imported] -
Arabidopsis thaliana, partial (53%)
Length = 1241
Score = 28.5 bits (62), Expect = 3.1
Identities = 21/63 (33%), Positives = 28/63 (44%), Gaps = 6/63 (9%)
Frame = +1
Query: 181 LRMNMGLKKLQERVKKQQET--VGKKIG----TVRRIAGMNRTEWVENADRLVAGFLEMF 234
L LK L E+ KK + KK G TVRR+AG R WV + + ++
Sbjct: 79 LYKEQNLKTLVEKFKKASDIDRFRKKNGIYEDTVRRLAGAKRFRWVRDIIEHQKSYADIS 258
Query: 235 EEG 237
EG
Sbjct: 259 NEG 267
>TC86742 similar to PIR|T01541|T01541 hypothetical protein A_IG005I10.16 -
Arabidopsis thaliana, partial (19%)
Length = 2073
Score = 28.5 bits (62), Expect = 3.1
Identities = 16/55 (29%), Positives = 27/55 (49%)
Frame = -1
Query: 134 ETKRTEGISTSDIIMRIIKDYNQYVMRNLDRGYSRKELGVSYVKEKRLRMNMGLK 188
E ++ E ++++++ +MR L RG R E VKE++ R GLK
Sbjct: 843 EVEKEECSGVKRRLLKVVERVEPVLMR*LSRGEKRWEEMEMVVKERKTRREKGLK 679
>TC92129 homologue to PIR|H84807|H84807 probable phospholipid
cytidylyltransferase [imported] - Arabidopsis thaliana,
partial (14%)
Length = 510
Score = 28.1 bits (61), Expect = 4.0
Identities = 10/16 (62%), Positives = 12/16 (74%)
Frame = +1
Query: 18 VYADGIYDLFHFGHAR 33
VY DG +DLFH GH +
Sbjct: 82 VYIDGAFDLFHAGHVQ 129
>TC80424 homologue to GP|21593052|gb|AAM65001.1 DAG protein putative
{Arabidopsis thaliana}, partial (57%)
Length = 1039
Score = 27.3 bits (59), Expect = 6.9
Identities = 22/76 (28%), Positives = 30/76 (38%), Gaps = 5/76 (6%)
Frame = +2
Query: 34 SLEQAKKSF----PNTYLLVGCCSDEIT-HKYKGKTVMNEQERYESLRHCKWVDEVIPDV 88
S+E+AKK+ TY C DE T K+KG +P V
Sbjct: 281 SMEEAKKNMYAFSTTTYTGFQCTVDEATSEKFKG----------------------LPGV 394
Query: 89 PWVINQEFIDKHKIDY 104
WV+ +ID +DY
Sbjct: 395 LWVLPDSYIDVKNMDY 442
>TC84306 weakly similar to PIR|T01047|T01047 hypothetical protein
YUP8H12R.30 - Arabidopsis thaliana, partial (10%)
Length = 666
Score = 27.3 bits (59), Expect = 6.9
Identities = 15/45 (33%), Positives = 23/45 (50%), Gaps = 1/45 (2%)
Frame = +3
Query: 9 VGKNDKPVRVYADGI-YDLFHFGHARSLEQAKKSFPNTYLLVGCC 52
VGK +KP A + YDL G AR + + ++ ++ GCC
Sbjct: 519 VGKGNKPEVTQATQLLYDLCRSGKARKAVRVMEIMVSSGIIPGCC 653
>BG451797 similar to GP|18028626|gb| maturase {Zippelia begoniifolia},
partial (2%)
Length = 383
Score = 27.3 bits (59), Expect = 6.9
Identities = 12/40 (30%), Positives = 19/40 (47%)
Frame = -2
Query: 52 CSDEITHKYKGKTVMNEQERYESLRHCKWVDEVIPDVPWV 91
C + H YK +T R+E L C ++ V +V W+
Sbjct: 184 CYKK*NHSYKNRTGNRWYVRFEQLNGCCAIEPVKTNVAWI 65
>CA918758 weakly similar to PIR|S74637|S746 lysine decarboxylase cad -
Synechocystis sp. (strain PCC 6803), partial (9%)
Length = 664
Score = 26.9 bits (58), Expect = 9.0
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = -3
Query: 86 PDVPWVINQEFIDKHKIDYVAHDALPYADTSGAGN 120
P +P +I E I + +DY+ H AD SGA +
Sbjct: 281 PGIPVLIPGEVITEKAVDYLLHVRSEGADISGASD 177
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.136 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,439,963
Number of Sequences: 36976
Number of extensions: 89221
Number of successful extensions: 546
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 517
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 535
length of query: 285
length of database: 9,014,727
effective HSP length: 95
effective length of query: 190
effective length of database: 5,502,007
effective search space: 1045381330
effective search space used: 1045381330
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC131248.8