BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC131240.2 + phase: 0
(90 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87104 weakly similar to GP|10176854|dbj|BAB10060. pectinacetyl... 169 2e-43
TC88870 similar to PIR|S68805|S68805 pectin acetylesterase (EC 3... 62 4e-11
TC76956 similar to PIR|S68805|S68805 pectin acetylesterase (EC 3... 57 1e-09
TC77757 weakly similar to PIR|A96610|A96610 probable pectinacety... 57 1e-09
TC90898 similar to PIR|A96610|A96610 probable pectinacetylestera... 56 2e-09
TC76957 similar to PIR|S68805|S68805 pectin acetylesterase (EC 3... 56 2e-09
TC77849 similar to PIR|A96610|A96610 probable pectinacetylestera... 54 8e-09
TC83626 similar to PIR|T01197|T01197 pectin acetylesterase homol... 53 2e-08
AL375964 similar to GP|10176999|dbj pectin acetylesterase {Arabi... 50 1e-07
TC87235 weakly similar to PIR|S68805|S68805 pectin acetylesteras... 50 1e-07
AW736314 weakly similar to PIR|S68805|S688 pectin acetylesterase... 47 1e-06
TC80293 homologue to GP|15292863|gb|AAK92802.1 unknown protein {... 29 0.27
AL374751 GP|2584797|emb| MtN28 {Medicago truncatula}, partial (25%) 27 1.8
BQ135869 26 2.3
CB893018 weakly similar to GP|15028251|gb unknown protein {Arabi... 26 3.0
TC86034 similar to GP|6815067|dbj|BAA90354.1 ESTs AU082210(C5365... 25 3.9
TC76480 similar to GP|11320850|dbj|BAB18333. contains ESTs AU094... 25 6.7
AL386407 25 6.7
BF647566 similar to GP|9294624|dbj DNA repair protein RAD54-like... 24 8.8
BF633915 24 8.8
>TC87104 weakly similar to GP|10176854|dbj|BAB10060. pectinacetylesterase
{Arabidopsis thaliana}, partial (73%)
Length = 1499
Score = 169 bits (427), Expect = 2e-43
Identities = 80/80 (100%), Positives = 80/80 (100%)
Frame = +2
Query: 1 MIHNSFSVTMNFAVAFALFYLITVESWRVHSQEPKKLYVNMTLVNNARETGAFCLDGSLP 60
MIHNSFSVTMNFAVAFALFYLITVESWRVHSQEPKKLYVNMTLVNNARETGAFCLDGSLP
Sbjct: 455 MIHNSFSVTMNFAVAFALFYLITVESWRVHSQEPKKLYVNMTLVNNARETGAFCLDGSLP 634
Query: 61 AYHLDRGFGAGEDNWLLQFE 80
AYHLDRGFGAGEDNWLLQFE
Sbjct: 635 AYHLDRGFGAGEDNWLLQFE 694
>TC88870 similar to PIR|S68805|S68805 pectin acetylesterase (EC 3.1.1.-)
precursor - mung bean, partial (93%)
Length = 1409
Score = 62.0 bits (149), Expect = 4e-11
Identities = 27/43 (62%), Positives = 34/43 (78%)
Frame = +3
Query: 38 YVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
YV +TL+ +A GA CLDGS PAYHLD+GFG G ++WL+QFE
Sbjct: 312 YVPITLLKSAVSKGAVCLDGSPPAYHLDKGFGTGINSWLVQFE 440
>TC76956 similar to PIR|S68805|S68805 pectin acetylesterase (EC 3.1.1.-)
precursor - mung bean, partial (93%)
Length = 1573
Score = 57.0 bits (136), Expect = 1e-09
Identities = 34/78 (43%), Positives = 45/78 (57%)
Frame = +3
Query: 3 HNSFSVTMNFAVAFALFYLITVESWRVHSQEPKKLYVNMTLVNNARETGAFCLDGSLPAY 62
H+SF+ M F LF L+ + + E + V +T V +A GA CLDGS PAY
Sbjct: 105 HSSFN*-MKFIRMDQLFSLVICTLLLLKAAEG--VTVPITFVQSAVAKGAVCLDGSPPAY 275
Query: 63 HLDRGFGAGEDNWLLQFE 80
H D+GF AG DNW++ FE
Sbjct: 276 HFDKGFEAGIDNWIVHFE 329
>TC77757 weakly similar to PIR|A96610|A96610 probable pectinacetylesterase
precursor T8L23.6 [imported] - Arabidopsis thaliana,
partial (86%)
Length = 1536
Score = 57.0 bits (136), Expect = 1e-09
Identities = 23/42 (54%), Positives = 31/42 (73%)
Frame = +2
Query: 39 VNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
V++TL+ A GA CLDG+LP YHLDRGF +G ++WL+ E
Sbjct: 338 VDLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLE 463
>TC90898 similar to PIR|A96610|A96610 probable pectinacetylesterase
precursor T8L23.6 [imported] - Arabidopsis thaliana,
partial (18%)
Length = 579
Score = 56.2 bits (134), Expect = 2e-09
Identities = 22/42 (52%), Positives = 31/42 (73%)
Frame = +1
Query: 39 VNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
V +TL+ +A GA CLDG+LPAYH D G+G+G ++WL+ E
Sbjct: 352 VGITLIQSAAAKGAVCLDGTLPAYHFDHGYGSGANSWLVNLE 477
>TC76957 similar to PIR|S68805|S68805 pectin acetylesterase (EC 3.1.1.-)
precursor - mung bean, partial (68%)
Length = 858
Score = 56.2 bits (134), Expect = 2e-09
Identities = 25/42 (59%), Positives = 29/42 (68%)
Frame = +3
Query: 39 VNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
V T V +A GA CLDGS PAYH D+GF AG DNW++ FE
Sbjct: 24 VPFTFVQSAVAKGAVCLDGSPPAYHFDKGFEAGIDNWIVHFE 149
>TC77849 similar to PIR|A96610|A96610 probable pectinacetylesterase
precursor T8L23.6 [imported] - Arabidopsis thaliana,
partial (86%)
Length = 1589
Score = 54.3 bits (129), Expect = 8e-09
Identities = 22/47 (46%), Positives = 32/47 (67%)
Frame = +3
Query: 34 PKKLYVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
P+ L + +TL++ A GA CLDG+LP YH G G+G ++WL+Q E
Sbjct: 234 PQPLMIPLTLIHGAVSKGAVCLDGTLPGYHFHPGSGSGANSWLIQLE 374
>TC83626 similar to PIR|T01197|T01197 pectin acetylesterase homolog
F21E10.11 - Arabidopsis thaliana, partial (9%)
Length = 562
Score = 52.8 bits (125), Expect = 2e-08
Identities = 22/40 (55%), Positives = 29/40 (72%)
Frame = +2
Query: 35 KKLYVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDN 74
+ L V +TL+N A GA CLDGSLPAYH RG+G+G ++
Sbjct: 419 RPLMVGLTLINGAAAKGAVCLDGSLPAYHFHRGYGSGSNS 538
>AL375964 similar to GP|10176999|dbj pectin acetylesterase {Arabidopsis
thaliana}, partial (24%)
Length = 515
Score = 50.1 bits (118), Expect = 1e-07
Identities = 21/43 (48%), Positives = 28/43 (64%)
Frame = +3
Query: 38 YVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
YV +T + +A GA CLDGS PAYH D+G G +NW++ E
Sbjct: 105 YVPLTRLESAVSKGAVCLDGSPPAYHFDQGHDEGANNWIVHIE 233
>TC87235 weakly similar to PIR|S68805|S68805 pectin acetylesterase (EC
3.1.1.-) precursor - mung bean, partial (89%)
Length = 1447
Score = 50.1 bits (118), Expect = 1e-07
Identities = 21/43 (48%), Positives = 28/43 (64%)
Frame = +3
Query: 38 YVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
YV +T + +A GA CLDGS PAYH D+G G +NW++ E
Sbjct: 120 YVPLTRLESAVSKGAVCLDGSPPAYHFDQGHDEGANNWIVHIE 248
>AW736314 weakly similar to PIR|S68805|S688 pectin acetylesterase (EC
3.1.1.-) precursor - mung bean, partial (13%)
Length = 203
Score = 47.0 bits (110), Expect = 1e-06
Identities = 22/42 (52%), Positives = 27/42 (63%)
Frame = +2
Query: 39 VNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
V +T +A GA LDG PAYH D+GF AG DNW++ FE
Sbjct: 65 VPITFDQSASA*GAV*LDGRPPAYHCDQGFQAGNDNWIVHFE 190
>TC80293 homologue to GP|15292863|gb|AAK92802.1 unknown protein {Arabidopsis
thaliana}, partial (10%)
Length = 703
Score = 29.3 bits (64), Expect = 0.27
Identities = 10/29 (34%), Positives = 20/29 (68%)
Frame = +3
Query: 5 SFSVTMNFAVAFALFYLITVESWRVHSQE 33
SFS+T N +++F F+ IT + ++H+ +
Sbjct: 195 SFSITKNLSISFIYFFKITAKHRKIHANK 281
>AL374751 GP|2584797|emb| MtN28 {Medicago truncatula}, partial (25%)
Length = 578
Score = 26.6 bits (57), Expect = 1.8
Identities = 12/31 (38%), Positives = 20/31 (63%)
Frame = +2
Query: 14 VAFALFYLITVESWRVHSQEPKKLYVNMTLV 44
VA AL+YL + W + + P +LYV++ L+
Sbjct: 299 VALALYYLYSSHYWGLWLKFPIELYVSIYLI 391
>BQ135869
Length = 791
Score = 26.2 bits (56), Expect = 2.3
Identities = 19/75 (25%), Positives = 36/75 (47%), Gaps = 2/75 (2%)
Frame = -3
Query: 18 LFYLITVESWRVHSQEPKKLYVNMTLVNNARETGAFCLDGSLPAY--HLDRGFGAGEDNW 75
+F+L+ + S R+ SQE K+ + + R++G LD + HLD+ G N
Sbjct: 777 IFFLLGIRSKRISSQEKKRFCMKQVFI---RKSGFL*LDPRQNNFLIHLDK-TGL*VPNK 610
Query: 76 LLQFEVYSYFSHCSV 90
+L+ + + C +
Sbjct: 609 VLK*SKFGMYGQCKI 565
>CB893018 weakly similar to GP|15028251|gb unknown protein {Arabidopsis
thaliana}, partial (39%)
Length = 582
Score = 25.8 bits (55), Expect = 3.0
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = +2
Query: 67 GFGAGEDNWLLQFEVYSYFS 86
G GAG NWL F V Y S
Sbjct: 197 GIGAGLGNWLYDFTVLCYHS 256
>TC86034 similar to GP|6815067|dbj|BAA90354.1 ESTs AU082210(C53655)
AU056546(S20671) AU056545(S20671) correspond to a region
of the predicted, partial (42%)
Length = 2412
Score = 25.4 bits (54), Expect = 3.9
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +3
Query: 16 FALFYLITVESWRVHSQEPKKLYVNMTL 43
FALF TVES + +EP K++ TL
Sbjct: 1008 FALFVYKTVESAKKALEEPNKVFEGHTL 1091
>TC76480 similar to GP|11320850|dbj|BAB18333. contains ESTs AU094466(E10996)
C19834(E10996)~similar to Arabidopsis thaliana
chromosome 3 T2E22., partial (67%)
Length = 1127
Score = 24.6 bits (52), Expect = 6.7
Identities = 12/48 (25%), Positives = 22/48 (45%)
Frame = -3
Query: 25 ESWRVHSQEPKKLYVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGE 72
E VH Q+ +++ + N G+F L +LP+ + G G+
Sbjct: 756 ECCHVHGQKEVLKFLHNEMANAPTSLGSFSLPPTLPSTYQQSSTGEGQ 613
>AL386407
Length = 468
Score = 24.6 bits (52), Expect = 6.7
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = -3
Query: 8 VTMNFAVAFALFYLITVESWRVHS 31
V +FA+ F+ FY++ + SW S
Sbjct: 118 VEWHFAINFSSFYILNIWSWSQQS 47
>BF647566 similar to GP|9294624|dbj DNA repair protein RAD54-like
{Arabidopsis thaliana}, partial (12%)
Length = 669
Score = 24.3 bits (51), Expect = 8.8
Identities = 10/24 (41%), Positives = 13/24 (53%)
Frame = +1
Query: 63 HLDRGFGAGEDNWLLQFEVYSYFS 86
H R +G GED + +YSY S
Sbjct: 403 HFGR*YGFGEDTAVHHITIYSYLS 474
>BF633915
Length = 468
Score = 24.3 bits (51), Expect = 8.8
Identities = 12/32 (37%), Positives = 14/32 (43%)
Frame = -3
Query: 54 CLDGSLPAYHLDRGFGAGEDNWLLQFEVYSYF 85
C P +H G WLLQFE+ S F
Sbjct: 115 CRKNGPPPFHCSNGVPV---TWLLQFELSSLF 29
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.137 0.436
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,517,636
Number of Sequences: 36976
Number of extensions: 41787
Number of successful extensions: 262
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 262
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 262
length of query: 90
length of database: 9,014,727
effective HSP length: 66
effective length of query: 24
effective length of database: 6,574,311
effective search space: 157783464
effective search space used: 157783464
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)
Medicago: description of AC131240.2