Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC130807.9 + phase: 0 /pseudo
         (311 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BG644740 similar to PIR|A84460|A84 probable retroelement pol pol...    74  9e-21
BG644693 weakly similar to GP|18767374|g Putative 22 kDa kafirin...    96  2e-20
TC86737 weakly similar to GP|6683624|dbj|BAA89272.1 Pol {Alterna...    83  1e-16
AL366725                                                               59  3e-09
TC79627                                                                32  0.41
TC88532 similar to PIR|S45033|S45033 probable imbibition protein...    29  2.7
TC81162 weakly similar to PIR|T47673|T47673 hypothetical protein...    29  2.7
TC88387 similar to GP|13357253|gb|AAK20050.1 putative zinc finge...    28  4.5
TC87639 GP|9663153|emb|CAC01132.1 transport-secretion protein 2....    28  4.5
TC87954 similar to GP|21928168|gb|AAM78111.1 AT5g23040/MYJ24_3 {...    28  5.9
TC93065                                                                27  7.7

>BG644740 similar to PIR|A84460|A84 probable retroelement pol polyprotein
           [imported] - Arabidopsis thaliana, partial (4%)
          Length = 754

 Score = 74.3 bits (181), Expect(2) = 9e-21
 Identities = 33/50 (66%), Positives = 40/50 (80%)
 Frame = -1

Query: 261 KFVRPSVSPWGAPVFLVMKKDGSMRLCIDYRQLNKVTIKNRYPLPRIDDL 310
           +F +PS+SP GA +  V KKDG  R+CIDYRQ NKVT KN+YPLPRID+L
Sbjct: 271 RFQQPSISP*GAALLFVRKKDGYFRMCIDYRQFNKVTTKNKYPLPRIDNL 122



 Score = 43.1 bits (100), Expect(2) = 9e-21
 Identities = 26/62 (41%), Positives = 36/62 (57%)
 Frame = -2

Query: 201 VVCDFPEVLPDEIPDMPPKREVEFSIDLVPRTKPVSMAPYRMSASELAELKKRLEDLLEN 260
           V+  F  V PD  P +P +RE+ F IDL+  T+ +S  P  M  +EL +LK  L+D LE 
Sbjct: 450 VLKGFS*VFPDNFPVIPLEREIFFCIDLLLDTQLISNPP*LMDRTELKKLKI*LKDSLEK 271

Query: 261 KF 262
            F
Sbjct: 270 GF 265


>BG644693 weakly similar to GP|18767374|g Putative 22 kDa kafirin cluster;
           Ty3-Gypsy type {Oryza sativa}, partial (15%)
          Length = 716

 Score = 95.9 bits (237), Expect = 2e-20
 Identities = 46/100 (46%), Positives = 68/100 (68%)
 Frame = +2

Query: 211 DEIPDMPPKREVEFSIDLVPRTKPVSMAPYRMSASELAELKKRLEDLLENKFVRPSVSPW 270
           D +  +PP+ +++F IDL+P   P+ +  YR++  +L  LK +L+DLLE  F++PS+ P 
Sbjct: 2   DHLL*VPPEWKIDFGIDLLPNMNPI*IPSYRINPLKLKVLKLQLKDLLEKGFIQPSIYP* 181

Query: 271 GAPVFLVMKKDGSMRLCIDYRQLNKVTIKNRYPLPRIDDL 310
           G  V  + KKDG +R+ IDY QLN V IK +YPLP ID+L
Sbjct: 182 GVVVLFLKKKDGFLRMSIDYPQLNNVNIKIKYPLPLIDEL 301


>TC86737 weakly similar to GP|6683624|dbj|BAA89272.1 Pol {Alternaria
           alternata}, partial (21%)
          Length = 1540

 Score = 83.2 bits (204), Expect = 1e-16
 Identities = 49/118 (41%), Positives = 70/118 (58%), Gaps = 7/118 (5%)
 Frame = +1

Query: 201 VVCDFPEVL-PDEIPDMPPKREV-EFSIDLVP----RTKPVSMAP-YRMSASELAELKKR 253
           V+ +FP++  P++   +P  R + + +I L+P       P+   P Y MS  EL  LKK 
Sbjct: 343 VLEEFPDLFNPEKAYQVPASRGLLDHAIPLIPDKDGNDPPLPWGPLYGMSRQELLVLKKT 522

Query: 254 LEDLLENKFVRPSVSPWGAPVFLVMKKDGSMRLCIDYRQLNKVTIKNRYPLPRIDDLM 311
           LEDLL+  F++ S S  GAPV  V K  G +R C+DYR LN +T K+RYPLP I + +
Sbjct: 523 LEDLLDKGFIKASGSAAGAPVLFVRKPGGGIRFCVDYRALNAITKKDRYPLPLISETL 696


>AL366725 
          Length = 485

 Score = 58.5 bits (140), Expect = 3e-09
 Identities = 24/24 (100%), Positives = 24/24 (100%)
 Frame = +2

Query: 1   RNDIVCFNCNEEGHIGSQCKQPKR 24
           RNDIVCFNCNEEGHIGSQCKQPKR
Sbjct: 413 RNDIVCFNCNEEGHIGSQCKQPKR 484


>TC79627 
          Length = 1522

 Score = 31.6 bits (70), Expect = 0.41
 Identities = 20/75 (26%), Positives = 35/75 (46%), Gaps = 7/75 (9%)
 Frame = +2

Query: 122  VCLHLSGMDVILGMNWLEYNHVHINCFSKSVC-------FSSAEEESGA*FLSTKQLKQM 174
            +C    GMD++L   W+ YN+  ++C     C       FS+ E+   A  L  K    +
Sbjct: 950  ICRTARGMDLMLV--WIAYNYSLVSCIHAGFCFLLPTVVFSTLEDNPAANLL*HKDSFNL 1123

Query: 175  ERDGILMFSLMASLS 189
              + + MF+L+ + S
Sbjct: 1124 VPEVVRMFNLLVNTS 1168


>TC88532 similar to PIR|S45033|S45033 probable imbibition protein - wild
           cabbage, partial (33%)
          Length = 1273

 Score = 28.9 bits (63), Expect = 2.7
 Identities = 16/52 (30%), Positives = 26/52 (49%), Gaps = 6/52 (11%)
 Frame = +1

Query: 73  DCVSALGLDLSDMNGEMVVETPTKGSVTTSLVCLRC------PLSMFGHDFE 118
           DC+      L   N  ++ + P+KG +  SL  L+C      P+ +FGHD +
Sbjct: 727 DCI------LYAFNAGLLSKLPSKGKLEVSLETLQCEVYTVSPIRVFGHDVQ 864


>TC81162 weakly similar to PIR|T47673|T47673 hypothetical protein T26I12.220
           - Arabidopsis thaliana, partial (9%)
          Length = 756

 Score = 28.9 bits (63), Expect = 2.7
 Identities = 10/28 (35%), Positives = 18/28 (63%)
 Frame = +2

Query: 1   RNDIVCFNCNEEGHIGSQCKQPKRAPTT 28
           R + +C+ C ++GH  S+C  P+ A +T
Sbjct: 407 RKNRMCYGCRQKGHNLSECPNPQIAAST 490


>TC88387 similar to GP|13357253|gb|AAK20050.1 putative zinc finger protein
           {Oryza sativa (japonica cultivar-group)}, partial (96%)
          Length = 1286

 Score = 28.1 bits (61), Expect = 4.5
 Identities = 10/26 (38%), Positives = 14/26 (53%)
 Frame = +1

Query: 5   VCFNCNEEGHIGSQCKQPKRAPTTGR 30
           +C+NC E GH+ S C       T G+
Sbjct: 535 LCWNCKEPGHMASSCPNEGICHTCGK 612



 Score = 27.3 bits (59), Expect = 7.7
 Identities = 10/18 (55%), Positives = 12/18 (66%)
 Frame = +1

Query: 2   NDIVCFNCNEEGHIGSQC 19
           N  VC NC+  GHI S+C
Sbjct: 469 NVAVCHNCSLPGHIASEC 522


>TC87639 GP|9663153|emb|CAC01132.1 transport-secretion protein 2.2 (TTS-2.2)
           {Homo sapiens}, partial (2%)
          Length = 1522

 Score = 28.1 bits (61), Expect = 4.5
 Identities = 11/32 (34%), Positives = 17/32 (52%), Gaps = 6/32 (18%)
 Frame = -1

Query: 6   CFNCNEEGHIGSQC------KQPKRAPTTGRV 31
           C++C+E GHI   C      K+ K+ P   +V
Sbjct: 316 CYSCHERGHIARNCTNTSAAKKKKKGPKVAKV 221


>TC87954 similar to GP|21928168|gb|AAM78111.1 AT5g23040/MYJ24_3 {Arabidopsis
           thaliana}, partial (82%)
          Length = 1200

 Score = 27.7 bits (60), Expect = 5.9
 Identities = 11/18 (61%), Positives = 13/18 (72%)
 Frame = -3

Query: 14  HIGSQCKQPKRAPTTGRV 31
           H+GS CKQPKR P   +V
Sbjct: 754 HLGSICKQPKRLPPEKQV 701


>TC93065 
          Length = 783

 Score = 27.3 bits (59), Expect = 7.7
 Identities = 13/52 (25%), Positives = 24/52 (46%)
 Frame = +2

Query: 1   RNDIVCFNCNEEGHIGSQCKQPKRAPTTGRVFAMAGTQTENEDRLIRGTCYI 52
           R  + C  CN+ GH+   CK   +     +   +     E+E+ L + +CY+
Sbjct: 620 RPGVKCRACNQLGHVEKVCK--NKTNQQEQEARVVEHHQEDEEPLFKASCYL 769


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.326    0.140    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,083,451
Number of Sequences: 36976
Number of extensions: 137618
Number of successful extensions: 959
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 939
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 958
length of query: 311
length of database: 9,014,727
effective HSP length: 96
effective length of query: 215
effective length of database: 5,465,031
effective search space: 1174981665
effective search space used: 1174981665
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)


Medicago: description of AC130807.9