BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC130807.4 - phase: 0
(329 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF003873 similar to GP|14715222|em putative polyprotein {Cicer a... 182 2e-46
TC77595 weakly similar to PIR|T18350|T18350 probable pol polypro... 119 2e-27
BG454871 weakly similar to GP|10140673|g putative gag-pol polypr... 73 3e-17
BG644699 similar to PIR|T07863|T078 probable polyprotein - pinea... 62 3e-10
BG587176 weakly similar to PIR|G84493|G84 probable retroelement ... 39 0.003
BG644733 weakly similar to GP|15289942|db putative polyprotein {... 36 0.023
BF636649 similar to GP|21628724|emb OSJNBa0033H08.7 {Oryza sativ... 35 0.031
BG587101 similar to GP|6691191|gb F7F22.15 {Arabidopsis thaliana... 34 0.068
BG586308 weakly similar to PIR|F84528|F8 probable retroelement p... 32 0.34
TC89832 homologue to GP|21618319|gb|AAM67369.1 unknown {Arabidop... 31 0.58
CA858686 homologue to GP|8489871|gb| DEAD box protein P68 {Pisum... 31 0.76
TC78917 weakly similar to GP|11385425|gb|AAG34795.1 glutathione ... 29 2.2
TC87772 weakly similar to GP|8574455|gb|AAF77578.1| pepper ester... 29 2.9
BF647164 weakly similar to GP|6691191|gb F7F22.15 {Arabidopsis t... 28 3.8
BQ135590 similar to GP|23499166|em hypothetical protein {Plasmod... 28 6.4
BE204912 similar to PIR|T02686|T026 probable Ser/Thr protein kin... 27 8.4
TC77637 similar to GP|15292697|gb|AAK92717.1 putative RNA-bindin... 27 8.4
BQ137251 similar to GP|18026135|gb| cytochrome c oxidase subunit... 27 8.4
>BF003873 similar to GP|14715222|em putative polyprotein {Cicer arietinum},
partial (82%)
Length = 559
Score = 182 bits (461), Expect = 2e-46
Identities = 94/125 (75%), Positives = 99/125 (79%), Gaps = 17/125 (13%)
Frame = +2
Query: 221 GVGHALKSRKLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHVSQLRKYVADPSHV 280
GVG ALKS+KLT +FIGPYQISERVGTVAYRVGLPPHL NLHDVFHVSQLRKYV DPSHV
Sbjct: 2 GVGRALKSKKLTVRFIGPYQISERVGTVAYRVGLPPHLLNLHDVFHVSQLRKYVPDPSHV 181
Query: 281 IPRDDVQVRDNLTVETL-----------------PLVKVVWDGATGESLTWELESKMRDS 323
I DDVQVRDNLTVETL PLV+VVWD A GESLTWELESKM +S
Sbjct: 182 IQSDDVQVRDNLTVETLPVRIDDRKVKTLRGKEIPLVRVVWDRANGESLTWELESKMVES 361
Query: 324 YPKLF 328
YP+LF
Sbjct: 362 YPELF 376
>TC77595 weakly similar to PIR|T18350|T18350 probable pol polyprotein - rice
blast fungus gypsy retroelement (fragment), partial
(14%)
Length = 1708
Score = 119 bits (298), Expect = 2e-27
Identities = 90/284 (31%), Positives = 137/284 (47%), Gaps = 7/284 (2%)
Frame = +2
Query: 1 MDFVMSLPNTT-RGHDSIWVVVDRLRKSAHFIPINIKYPVAQLAEIYIHNIVKLHGVPSS 59
MDF+ SLP T RG +WV+VDRL KS ++ A A+ ++ + HG+P S
Sbjct: 401 MDFITSLPPTRGRGSQYLWVIVDRLSKSVTLEEMDTMEAEA-CAQRFLSCHYRFHGMPQS 577
Query: 60 IVSDRDPRFTSRFWKSLQDALGSKLRLSSAYHPQTDGQSKRTIQSLEYLLRVCVLEEGGA 119
IVSDR + RFW+ G LS++YHPQTDG ++R Q ++ +LR V
Sbjct: 578 IVSDRGSNWVGRFWREFCRLTGVTQLLSTSYHPQTDGGTERWNQEIQAVLRAYVCWSQDN 757
Query: 120 WDSHLPLIEFTYNNSYHSSIGMAPFKALYGRRCRTPLCWFESGESVVLGPD-----LVHE 174
W LP ++ N ++SSIG PF +G P+ E VV + LV
Sbjct: 758 WGDLLPTVQLALRNRHNSSIGATPFFVEHGYHV-DPIPTVEDTGGVVSEGEAAAQLLVKR 934
Query: 175 TTEKVKMIRKKMKASQSRQKSYHDKRR-KALEFQEGDHVFLRVTPMTGVGHALKSRKLTP 233
+ I+ ++ A+Q R ++ +KRR A +Q GD V+L V+ + K L
Sbjct: 935 MKDVTGFIQAEIVAAQQRSEASANKRRCPADRYQVGDKVWLNVSNYKSPRPSKKLDWLHH 1114
Query: 234 KFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHVSQLRKYVADP 277
K Y+++ V + +P ++ FHV LR+ +DP
Sbjct: 1115K----YEVTRFVTPHVVELNVP---GTVYPKFHVDLLRRAASDP 1225
>BG454871 weakly similar to GP|10140673|g putative gag-pol polyprotein {Oryza
sativa (japonica cultivar-group)}, partial (7%)
Length = 674
Score = 73.2 bits (178), Expect(2) = 3e-17
Identities = 37/81 (45%), Positives = 46/81 (56%)
Frame = +2
Query: 70 SRFWKSLQDALGSKLRLSSAYHPQTDGQSKRTIQSLEYLLRVCVLEEGGAWDSHLPLIEF 129
S FWK L G+ L +SSAYHP +DGQS+ + E LR + + W P E+
Sbjct: 32 SNFWKQLFKLHGTILTMSSAYHP*SDGQSEALNKGXEMYLRCLMFTDPLKWSKAFPWAEY 211
Query: 130 TYNNSYHSSIGMAPFKALYGR 150
YN SY+ S M PFKALYGR
Sbjct: 212 WYNTSYNISAAMTPFKALYGR 274
Score = 32.7 bits (73), Expect(2) = 3e-17
Identities = 21/73 (28%), Positives = 34/73 (45%), Gaps = 2/73 (2%)
Frame = +1
Query: 189 SQSRQKSYHDKRRKALEFQEGDHVFLRVTPMTGVGHALKSRKL--TPKFIGPYQISERVG 246
+Q K DK+R+ EFQ G+HV +++ P AL+ + +P F +
Sbjct: 388 AQQTMKHQADKKRRHFEFQLGEHVLVKLQPYQQSSVALRKYQKFGSPNFGSLLTVCSL*V 567
Query: 247 TVAYRVGLPPHLS 259
A+ PP+LS
Sbjct: 568 ESAFHCKSPPYLS 606
>BG644699 similar to PIR|T07863|T078 probable polyprotein - pineapple
retrotransposon dea1 (fragment), partial (5%)
Length = 231
Score = 62.0 bits (149), Expect = 3e-10
Identities = 30/73 (41%), Positives = 47/73 (64%), Gaps = 1/73 (1%)
Frame = +2
Query: 210 DHVFLRVTPMT-GVGHALKSRKLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHVS 268
+ V L+V P G K KL+ ++IGP+++ +R+G VAY + LPP LS +H VFHVS
Sbjct: 2 EQVLLKVLPTERGDCRFGKRGKLSLRYIGPFEVIKRIGEVAYELALPPGLSGVHPVFHVS 181
Query: 269 QLRKYVADPSHVI 281
++Y D +++I
Sbjct: 182 MFKRYHGDGNYII 220
>BG587176 weakly similar to PIR|G84493|G84 probable retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(1%)
Length = 729
Score = 38.9 bits (89), Expect = 0.003
Identities = 14/32 (43%), Positives = 23/32 (71%)
Frame = -1
Query: 297 LPLVKVVWDGATGESLTWELESKMRDSYPKLF 328
+ +VK+VWD + E +TWE E++M+ YP+ F
Sbjct: 651 IQMVKIVWDCSGREEITWETEARMKADYPEWF 556
>BG644733 weakly similar to GP|15289942|db putative polyprotein {Oryza sativa
(japonica cultivar-group)}, partial (1%)
Length = 174
Score = 35.8 bits (81), Expect = 0.023
Identities = 19/43 (44%), Positives = 24/43 (55%)
Frame = -3
Query: 12 RGHDSIWVVVDRLRKSAHFIPINIKYPVAQLAEIYIHNIVKLH 54
+ ++SI VVVDRL KS FIP Y A I + IV +H
Sbjct: 130 KSYESI*VVVDRLTKSTLFIPFKTSYSAK*YARILLDEIVCIH 2
>BF636649 similar to GP|21628724|emb OSJNBa0033H08.7 {Oryza sativa}, partial
(4%)
Length = 653
Score = 35.4 bits (80), Expect = 0.031
Identities = 36/166 (21%), Positives = 67/166 (39%), Gaps = 4/166 (2%)
Frame = +1
Query: 94 TDGQSKRTIQSLEYLLRVCVLEEGGAWDSHLPLIEFTYNNSYHSSIGMAPFKALYGRRCR 153
+D Q+ +LE L E+ G + L E YN ++H + G PFK +Y
Sbjct: 7 SDEQAGLLNHTLETHLLYFTSEQQGV*NFFLTWAECLYNTNFHRTAGCTPFKVVY---VV 177
Query: 154 TPLCWFESGESVVLGPDLVHETTEKVKMIRKKMKASQSRQKSY----HDKRRKALEFQEG 209
L F ++ + +H+++ R + +Y H +R ++ +
Sbjct: 178 AHLQKFVVARDLIYRNEGLHKSST*TSFGRGTRAYEALSRPAYETC*HPRRPLSIVYTRD 357
Query: 210 DHVFLRVTPMTGVGHALKSRKLTPKFIGPYQISERVGTVAYRVGLP 255
+V P K GPYQ+ +++G+VA+++ LP
Sbjct: 358 RTYEWQVLP-----------KYVA*CYGPYQVIKQIGSVAFKL*LP 462
>BG587101 similar to GP|6691191|gb F7F22.15 {Arabidopsis thaliana}, partial
(10%)
Length = 624
Score = 34.3 bits (77), Expect = 0.068
Identities = 27/101 (26%), Positives = 49/101 (47%), Gaps = 2/101 (1%)
Frame = +2
Query: 1 MDFVMSLPNTTRGHDSIWVVVDRLRKSAHFI--PINIKYPVAQLAEIYIHNIVKLHGVPS 58
+DF+ P++ + I V VD + K I P N + +++ I GVP
Sbjct: 326 IDFMGPFPSSYN-NKYILVAVDYVSKWVEAIASPTN---DATVVVKMFKSVIFPRFGVPR 493
Query: 59 SIVSDRDPRFTSRFWKSLQDALGSKLRLSSAYHPQTDGQSK 99
++SD F ++ ++ L G + ++++AYHPQ +SK
Sbjct: 494 VVISDGGSHFINKVFEKLLKKNGVRHKVATAYHPQKAERSK 616
>BG586308 weakly similar to PIR|F84528|F8 probable retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(7%)
Length = 686
Score = 32.0 bits (71), Expect = 0.34
Identities = 18/71 (25%), Positives = 36/71 (50%)
Frame = -2
Query: 54 HGVPSSIVSDRDPRFTSRFWKSLQDALGSKLRLSSAYHPQTDGQSKRTIQSLEYLLRVCV 113
HG+P IV+D F S ++ + +L +S +PQ++GQ++ + + + L+ +
Sbjct: 685 HGLPYEIVTDNGSHFISNKFREFCERWRIRLNTASPRYPQSNGQAEASNKIIIDGLKKRL 506
Query: 114 LEEGGAWDSHL 124
+ G W L
Sbjct: 505 DLKKGCWADEL 473
>TC89832 homologue to GP|21618319|gb|AAM67369.1 unknown {Arabidopsis
thaliana}, partial (62%)
Length = 1347
Score = 31.2 bits (69), Expect = 0.58
Identities = 19/53 (35%), Positives = 25/53 (46%)
Frame = +1
Query: 48 HNIVKLHGVPSSIVSDRDPRFTSRFWKSLQDALGSKLRLSSAYHPQTDGQSKR 100
H +++G PS I R FW S+ LGS R Y P TDG ++R
Sbjct: 715 HWCPQIYGPPSKINKARQRTNVQVFWGSVH-GLGSFCRRCYCYSPDTDGIARR 870
>CA858686 homologue to GP|8489871|gb| DEAD box protein P68 {Pisum sativum},
partial (31%)
Length = 598
Score = 30.8 bits (68), Expect = 0.76
Identities = 25/92 (27%), Positives = 38/92 (41%), Gaps = 2/92 (2%)
Frame = +2
Query: 187 KASQSRQKSYHDKRRKALEFQEGDHVFLRVTPMTGVGHA--LKSRKLTPKFIGPYQISER 244
++ R+ + HD R + V R +TGV H L K T +I + R
Sbjct: 308 RSQSEREAALHDFRSSSTSILVATDVASRGLDVTGVSHVINLDLPKTTEDYIHRIGRTGR 487
Query: 245 VGTVAYRVGLPPHLSNLHDVFHVSQLRKYVAD 276
G+ G+ D+F VS +RK +AD
Sbjct: 488 AGS----TGIATSFYTDRDMFLVSNIRKAIAD 571
>TC78917 weakly similar to GP|11385425|gb|AAG34795.1 glutathione
S-transferase GST 5 {Glycine max}, partial (90%)
Length = 894
Score = 29.3 bits (64), Expect = 2.2
Identities = 19/67 (28%), Positives = 35/67 (51%), Gaps = 1/67 (1%)
Frame = +2
Query: 46 YIHNIVKLHGVPSSIVSDRDPRFTSRFWKS-LQDALGSKLRLSSAYHPQTDGQSKRTIQS 104
YI + K +G PS + S+ R ++RFW + + D L + LR + + + + K +
Sbjct: 245 YIDEVWKNNGTPSILPSNAYDRASARFWGAYIDDKLYNSLR-NGLFAQDIESKKKHFEEL 421
Query: 105 LEYLLRV 111
E LL++
Sbjct: 422 AEVLLKL 442
>TC87772 weakly similar to GP|8574455|gb|AAF77578.1| pepper esterase
{Capsicum annuum}, partial (34%)
Length = 1313
Score = 28.9 bits (63), Expect = 2.9
Identities = 16/43 (37%), Positives = 25/43 (57%), Gaps = 4/43 (9%)
Frame = -2
Query: 257 HLSNLHDVFHVSQLRKYVADPS----HVIPRDDVQVRDNLTVE 295
H+SN HDV + LR ++ S H +P D V V+D++ V+
Sbjct: 628 HVSNQHDVLAMDLLRF*SSEESPRSLHRLPEDGVPVQDDIRVK 500
>BF647164 weakly similar to GP|6691191|gb F7F22.15 {Arabidopsis thaliana},
partial (6%)
Length = 469
Score = 28.5 bits (62), Expect = 3.8
Identities = 20/66 (30%), Positives = 32/66 (48%)
Frame = +1
Query: 187 KASQSRQKSYHDKRRKALEFQEGDHVFLRVTPMTGVGHALKSRKLTPKFIGPYQISERVG 246
K + R K +HD+R EF+EG+ V L + + L KL + GP+Q+ +
Sbjct: 151 KIYKERTKKWHDRRIIRREFREGELVLLFNSRL-----KLFPGKLRSHWSGPFQVKNVMP 315
Query: 247 TVAYRV 252
+ A V
Sbjct: 316 SGAVEV 333
>BQ135590 similar to GP|23499166|em hypothetical protein {Plasmodium
falciparum 3D7}, partial (0%)
Length = 1238
Score = 27.7 bits (60), Expect = 6.4
Identities = 25/90 (27%), Positives = 43/90 (47%), Gaps = 1/90 (1%)
Frame = +1
Query: 3 FVMSLPNTTRGHDSIWVVVDRLRKSAHFIPINIKYPVAQLAEIYIHNIVKLHGVPSSIVS 62
F SLP T+G +++ +V + HF I + A +A ++ +N +IV
Sbjct: 400 FSSSLPPKTKGDENVPIVAREMSMQLHFFFITL--CSAFIALMFSNN*ENARA--CTIVD 567
Query: 63 DRDPRFTSRFWKSLQD-ALGSKLRLSSAYH 91
R+ + S W S+QD + S+ R + YH
Sbjct: 568 VREDKR*STQWWSVQDISSSSRARAAV*YH 657
>BE204912 similar to PIR|T02686|T026 probable Ser/Thr protein kinase
[imported] - Arabidopsis thaliana, partial (2%)
Length = 603
Score = 27.3 bits (59), Expect = 8.4
Identities = 18/47 (38%), Positives = 26/47 (55%), Gaps = 2/47 (4%)
Frame = +3
Query: 248 VAYRVGLPPHLS--NLHDVFHVSQLRKYVADPSHVIPRDDVQVRDNL 292
VAYR P LS N+ D+F++S LR +A S++ D + D L
Sbjct: 186 VAYRAQSPNFLSLSNISDIFNLSPLR--IAKASNIEAEDKKLIPDQL 320
>TC77637 similar to GP|15292697|gb|AAK92717.1 putative RNA-binding protein
{Arabidopsis thaliana}, partial (59%)
Length = 1724
Score = 27.3 bits (59), Expect = 8.4
Identities = 29/112 (25%), Positives = 45/112 (39%), Gaps = 7/112 (6%)
Frame = -3
Query: 152 CRTPLCWFESG---ESVVLGPDLVHETTEKVKMIRKKM----KASQSRQKSYHDKRRKAL 204
CR CW ESG S+ P + + I + A+ SR ++RR +
Sbjct: 1587 CRV*RCWCESGTTASSIPFSPQSITVVATSISKIATTL*HIVPATMSR-----NRRRTPV 1423
Query: 205 EFQEGDHVFLRVTPMTGVGHALKSRKLTPKFIGPYQISERVGTVAYRVGLPP 256
+ Q + F P+ +AL+ +L P P+ RV T + LPP
Sbjct: 1422 DTQYCQNAFPPTFPLPDPMYALEFAELVP*LRLPFPHKFRV-TELKKELLPP 1270
>BQ137251 similar to GP|18026135|gb| cytochrome c oxidase subunit II
{Sphaerospira fraseri}, partial (12%)
Length = 1073
Score = 27.3 bits (59), Expect = 8.4
Identities = 19/63 (30%), Positives = 27/63 (42%)
Frame = -3
Query: 85 RLSSAYHPQTDGQSKRTIQSLEYLLRVCVLEEGGAWDSHLPLIEFTYNNSYHSSIGMAPF 144
RLS + P D R + L LLR +W PL+EF ++ + G AP
Sbjct: 300 RLSCPFRPPPD-TGLRNLSFLPPLLRNFCFSVSLSWRQPSPLVEFRWSPRCAARAGRAPV 124
Query: 145 KAL 147
+L
Sbjct: 123 VSL 115
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.136 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,561,519
Number of Sequences: 36976
Number of extensions: 142812
Number of successful extensions: 714
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 708
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 711
length of query: 329
length of database: 9,014,727
effective HSP length: 96
effective length of query: 233
effective length of database: 5,465,031
effective search space: 1273352223
effective search space used: 1273352223
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC130807.4