
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127429.2 + phase: 2 /pseudo
(500 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI272114 212 2e-55
AL389604 similar to PIR|T39705|S673 probable diphthamide biosynt... 138 4e-33
BI265981 similar to GP|10176920|db gb|AAF50025.1~gene_id:MTG10.5... 42 4e-04
TC90508 homologue to PIR|JC1122|JC1122 14-3-3 protein - fruit fl... 34 0.15
TC86421 similar to GP|2407790|gb|AAB70660.1| grr1 {Glycine max},... 30 2.8
BQ147264 similar to GP|21805715|gb| hypothetical protein {Arabid... 29 4.8
TC81263 similar to GP|21689769|gb|AAM67528.1 putative SIR2-famil... 28 6.3
TC90246 similar to PIR|T00872|T00872 probable protein kinase At2... 28 8.2
BE204783 weakly similar to GP|18157659|gb cytochrome P450 {Eupho... 28 8.2
>BI272114
Length = 588
Score = 212 bits (540), Expect = 2e-55
Identities = 104/105 (99%), Positives = 105/105 (99%)
Frame = +3
Query: 396 QVQVGNQEEARFSFLKGGYVEDFENQENNVEEEREDLALANATEKALQLRDNCNSIIKGG 455
QVQVGNQEEARFSFLKGGYVEDFENQENNVEEEREDLALANATEKALQLRDNCNSII+GG
Sbjct: 3 QVQVGNQEEARFSFLKGGYVEDFENQENNVEEEREDLALANATEKALQLRDNCNSIIEGG 182
Query: 456 AKSGAEFFANRTYQGLNIPDDNTTPQSFVIGRKGRAAGYEDENNK 500
AKSGAEFFANRTYQGLNIPDDNTTPQSFVIGRKGRAAGYEDENNK
Sbjct: 183 AKSGAEFFANRTYQGLNIPDDNTTPQSFVIGRKGRAAGYEDENNK 317
>AL389604 similar to PIR|T39705|S673 probable diphthamide biosynthesis
protein - fission yeast (Schizosaccharomyces pombe),
partial (17%)
Length = 440
Score = 138 bits (348), Expect = 4e-33
Identities = 66/146 (45%), Positives = 97/146 (66%), Gaps = 4/146 (2%)
Frame = +3
Query: 271 QQRRILKRRYYLVERAKDANIVGILVGTLGVAGYLHIINQMMGLITAAGKKAYTLVMGKP 330
Q R L +RYY++++AKDA+++GI+VGTLG+A YL +I+ + LI AGKK YT VMGK
Sbjct: 3 QVNRALMKRYYMIQKAKDADVIGIVVGTLGIASYLTVISHLKKLIIHAGKKPYTFVMGKL 182
Query: 331 NPAKLANFPECDVFLYVSCAQTALLDSKDYLAPVITPFEATIAFNRGSQWTGAYVMGFGD 390
N AK+ANF E + F+ V+C + +L+DSK++ P++TPFE IA N QWTG Y+ F
Sbjct: 183 NVAKMANFMEIECFVLVACPENSLIDSKEFYRPIVTPFELEIALNPSRQWTGEYITDFQL 362
Query: 391 LINLPQVQV----GNQEEARFSFLKG 412
L++ + + +E FS + G
Sbjct: 363 LLSCNHSNIEEVGEDDDEPHFSLVTG 440
>BI265981 similar to GP|10176920|db gb|AAF50025.1~gene_id:MTG10.5~similar to
unknown protein {Arabidopsis thaliana}, partial (24%)
Length = 547
Score = 42.4 bits (98), Expect = 4e-04
Identities = 14/31 (45%), Positives = 22/31 (70%)
Frame = +2
Query: 66 FVMADTAYGSCCVDEVGASHINADCVIHYGH 96
+V+ D YG+CC+D+ A + A+ +IHYGH
Sbjct: 455 YVLGDVTYGACCIDDFSAIALGAEILIHYGH 547
>TC90508 homologue to PIR|JC1122|JC1122 14-3-3 protein - fruit fly
(Drosophila melanogaster), complete
Length = 1032
Score = 33.9 bits (76), Expect = 0.15
Identities = 18/36 (50%), Positives = 23/36 (63%)
Frame = +2
Query: 29 DDLLKDSTRVVSVLRKRLRSLRESDTTGSGDEPDIG 64
+D KDST ++ +LR L +L SDT G GDEP G
Sbjct: 788 EDSYKDSTLIMQLLRDNL-TLWTSDTQGDGDEPQEG 892
>TC86421 similar to GP|2407790|gb|AAB70660.1| grr1 {Glycine max}, partial
(74%)
Length = 2856
Score = 29.6 bits (65), Expect = 2.8
Identities = 19/76 (25%), Positives = 31/76 (40%), Gaps = 6/76 (7%)
Frame = +1
Query: 46 LRSLRESDTTGSGDEPDIGLFVMADTAYGSCCVDEVGASHINADCVIHYGHTC------F 99
L+++ D G GD+ GLF + + S ++ + HYG T F
Sbjct: 1024 LKAISIKDCAGVGDQGIAGLFSSTSLVLTKVKLQALAVSDLSLAVIGHYGKTVTDLVLNF 1203
Query: 100 SPTTTLPAFFVFGKAS 115
P + F+V G A+
Sbjct: 1204 LPNVSERGFWVMGNAN 1251
>BQ147264 similar to GP|21805715|gb| hypothetical protein {Arabidopsis
thaliana}, partial (15%)
Length = 646
Score = 28.9 bits (63), Expect = 4.8
Identities = 25/118 (21%), Positives = 46/118 (38%)
Frame = +1
Query: 77 CVDEVGASHINADCVIHYGHTCFSPTTTLPAFFVFGKASIGLADCVESMSQYALTSSKPI 136
C E + NA C + +TC SP P+ + + + S+ + L++ K +
Sbjct: 127 CFSENSINVSNASCSSYSNNTCISPNVVSPS----------IQNSISSVYKLVLSTLKHL 276
Query: 137 MVLYGLEYAHSIQQVRKALLESSTFCTSDPKSEVHFADVPSPYMFPSKDTKKLNDLQE 194
++ HS Q + S +F DP + F + F K K+ D ++
Sbjct: 277 LITVTWCKNHSNQGL------SISFGDEDPSTVPSFRLNTNSRFFRKKKGSKMLDFED 432
>TC81263 similar to GP|21689769|gb|AAM67528.1 putative SIR2-family protein
{Arabidopsis thaliana}, partial (76%)
Length = 1297
Score = 28.5 bits (62), Expect = 6.3
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = -3
Query: 133 SKPIMVLYGLEYAHSIQQVRKALLESSTFCTSDPKSEVHFADVPSPYMFPSKDTK 187
+K ++V Y E +I +R +++ +TFCT S+ H F DTK
Sbjct: 335 TKKLLVGYWSE-TRTISTIRASIVRDATFCTDSSSSQYHELGTVH*NFFQKIDTK 174
>TC90246 similar to PIR|T00872|T00872 probable protein kinase At2g45590
[imported] - Arabidopsis thaliana, partial (28%)
Length = 1451
Score = 28.1 bits (61), Expect = 8.2
Identities = 18/60 (30%), Positives = 30/60 (50%)
Frame = +2
Query: 180 MFPSKDTKKLNDLQEETCGCGDNSSSDGASGAAYSIGGLTWKLLEGQSMEDYSLFWIGHD 239
M S K+++ E+ G NS D + GLT ++ EG+ ++DY + WIG +
Sbjct: 50 MAASPGFDKVSEKSEKGKGLTSNSVRDWWWKQDNEVAGLT-QVGEGKKVKDYVMDWIGRE 226
>BE204783 weakly similar to GP|18157659|gb cytochrome P450 {Euphorbia
lagascae}, partial (34%)
Length = 599
Score = 28.1 bits (61), Expect = 8.2
Identities = 11/35 (31%), Positives = 22/35 (62%)
Frame = +2
Query: 388 FGDLINLPQVQVGNQEEARFSFLKGGYVEDFENQE 422
F +++ P++ QEE R +F+ GYV++ + +E
Sbjct: 23 FSEMLKNPRILKRAQEEVRHAFVSRGYVDEKDLEE 127
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.137 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,838,069
Number of Sequences: 36976
Number of extensions: 197048
Number of successful extensions: 820
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 815
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 819
length of query: 500
length of database: 9,014,727
effective HSP length: 100
effective length of query: 400
effective length of database: 5,317,127
effective search space: 2126850800
effective search space used: 2126850800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
Medicago: description of AC127429.2