Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC127429.2 + phase: 2 /pseudo
         (500 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BI272114                                                              212  2e-55
AL389604 similar to PIR|T39705|S673 probable diphthamide biosynt...   138  4e-33
BI265981 similar to GP|10176920|db gb|AAF50025.1~gene_id:MTG10.5...    42  4e-04
TC90508 homologue to PIR|JC1122|JC1122 14-3-3 protein - fruit fl...    34  0.15
TC86421 similar to GP|2407790|gb|AAB70660.1| grr1 {Glycine max},...    30  2.8
BQ147264 similar to GP|21805715|gb| hypothetical protein {Arabid...    29  4.8
TC81263 similar to GP|21689769|gb|AAM67528.1 putative SIR2-famil...    28  6.3
TC90246 similar to PIR|T00872|T00872 probable protein kinase At2...    28  8.2
BE204783 weakly similar to GP|18157659|gb cytochrome P450 {Eupho...    28  8.2

>BI272114 
          Length = 588

 Score =  212 bits (540), Expect = 2e-55
 Identities = 104/105 (99%), Positives = 105/105 (99%)
 Frame = +3

Query: 396 QVQVGNQEEARFSFLKGGYVEDFENQENNVEEEREDLALANATEKALQLRDNCNSIIKGG 455
           QVQVGNQEEARFSFLKGGYVEDFENQENNVEEEREDLALANATEKALQLRDNCNSII+GG
Sbjct: 3   QVQVGNQEEARFSFLKGGYVEDFENQENNVEEEREDLALANATEKALQLRDNCNSIIEGG 182

Query: 456 AKSGAEFFANRTYQGLNIPDDNTTPQSFVIGRKGRAAGYEDENNK 500
           AKSGAEFFANRTYQGLNIPDDNTTPQSFVIGRKGRAAGYEDENNK
Sbjct: 183 AKSGAEFFANRTYQGLNIPDDNTTPQSFVIGRKGRAAGYEDENNK 317


>AL389604 similar to PIR|T39705|S673 probable diphthamide biosynthesis
           protein - fission yeast (Schizosaccharomyces pombe),
           partial (17%)
          Length = 440

 Score =  138 bits (348), Expect = 4e-33
 Identities = 66/146 (45%), Positives = 97/146 (66%), Gaps = 4/146 (2%)
 Frame = +3

Query: 271 QQRRILKRRYYLVERAKDANIVGILVGTLGVAGYLHIINQMMGLITAAGKKAYTLVMGKP 330
           Q  R L +RYY++++AKDA+++GI+VGTLG+A YL +I+ +  LI  AGKK YT VMGK 
Sbjct: 3   QVNRALMKRYYMIQKAKDADVIGIVVGTLGIASYLTVISHLKKLIIHAGKKPYTFVMGKL 182

Query: 331 NPAKLANFPECDVFLYVSCAQTALLDSKDYLAPVITPFEATIAFNRGSQWTGAYVMGFGD 390
           N AK+ANF E + F+ V+C + +L+DSK++  P++TPFE  IA N   QWTG Y+  F  
Sbjct: 183 NVAKMANFMEIECFVLVACPENSLIDSKEFYRPIVTPFELEIALNPSRQWTGEYITDFQL 362

Query: 391 LINLPQVQV----GNQEEARFSFLKG 412
           L++     +     + +E  FS + G
Sbjct: 363 LLSCNHSNIEEVGEDDDEPHFSLVTG 440


>BI265981 similar to GP|10176920|db gb|AAF50025.1~gene_id:MTG10.5~similar to
           unknown protein {Arabidopsis thaliana}, partial (24%)
          Length = 547

 Score = 42.4 bits (98), Expect = 4e-04
 Identities = 14/31 (45%), Positives = 22/31 (70%)
 Frame = +2

Query: 66  FVMADTAYGSCCVDEVGASHINADCVIHYGH 96
           +V+ D  YG+CC+D+  A  + A+ +IHYGH
Sbjct: 455 YVLGDVTYGACCIDDFSAIALGAEILIHYGH 547


>TC90508 homologue to PIR|JC1122|JC1122 14-3-3 protein - fruit fly
           (Drosophila melanogaster), complete
          Length = 1032

 Score = 33.9 bits (76), Expect = 0.15
 Identities = 18/36 (50%), Positives = 23/36 (63%)
 Frame = +2

Query: 29  DDLLKDSTRVVSVLRKRLRSLRESDTTGSGDEPDIG 64
           +D  KDST ++ +LR  L +L  SDT G GDEP  G
Sbjct: 788 EDSYKDSTLIMQLLRDNL-TLWTSDTQGDGDEPQEG 892


>TC86421 similar to GP|2407790|gb|AAB70660.1| grr1 {Glycine max}, partial
            (74%)
          Length = 2856

 Score = 29.6 bits (65), Expect = 2.8
 Identities = 19/76 (25%), Positives = 31/76 (40%), Gaps = 6/76 (7%)
 Frame = +1

Query: 46   LRSLRESDTTGSGDEPDIGLFVMADTAYGSCCVDEVGASHINADCVIHYGHTC------F 99
            L+++   D  G GD+   GLF           +  +  S ++   + HYG T       F
Sbjct: 1024 LKAISIKDCAGVGDQGIAGLFSSTSLVLTKVKLQALAVSDLSLAVIGHYGKTVTDLVLNF 1203

Query: 100  SPTTTLPAFFVFGKAS 115
             P  +   F+V G A+
Sbjct: 1204 LPNVSERGFWVMGNAN 1251


>BQ147264 similar to GP|21805715|gb| hypothetical protein {Arabidopsis
           thaliana}, partial (15%)
          Length = 646

 Score = 28.9 bits (63), Expect = 4.8
 Identities = 25/118 (21%), Positives = 46/118 (38%)
 Frame = +1

Query: 77  CVDEVGASHINADCVIHYGHTCFSPTTTLPAFFVFGKASIGLADCVESMSQYALTSSKPI 136
           C  E   +  NA C  +  +TC SP    P+          + + + S+ +  L++ K +
Sbjct: 127 CFSENSINVSNASCSSYSNNTCISPNVVSPS----------IQNSISSVYKLVLSTLKHL 276

Query: 137 MVLYGLEYAHSIQQVRKALLESSTFCTSDPKSEVHFADVPSPYMFPSKDTKKLNDLQE 194
           ++       HS Q +      S +F   DP +   F    +   F  K   K+ D ++
Sbjct: 277 LITVTWCKNHSNQGL------SISFGDEDPSTVPSFRLNTNSRFFRKKKGSKMLDFED 432


>TC81263 similar to GP|21689769|gb|AAM67528.1 putative SIR2-family protein
           {Arabidopsis thaliana}, partial (76%)
          Length = 1297

 Score = 28.5 bits (62), Expect = 6.3
 Identities = 16/55 (29%), Positives = 26/55 (47%)
 Frame = -3

Query: 133 SKPIMVLYGLEYAHSIQQVRKALLESSTFCTSDPKSEVHFADVPSPYMFPSKDTK 187
           +K ++V Y  E   +I  +R +++  +TFCT    S+ H         F   DTK
Sbjct: 335 TKKLLVGYWSE-TRTISTIRASIVRDATFCTDSSSSQYHELGTVH*NFFQKIDTK 174


>TC90246 similar to PIR|T00872|T00872 probable protein kinase At2g45590
           [imported] - Arabidopsis thaliana, partial (28%)
          Length = 1451

 Score = 28.1 bits (61), Expect = 8.2
 Identities = 18/60 (30%), Positives = 30/60 (50%)
 Frame = +2

Query: 180 MFPSKDTKKLNDLQEETCGCGDNSSSDGASGAAYSIGGLTWKLLEGQSMEDYSLFWIGHD 239
           M  S    K+++  E+  G   NS  D        + GLT ++ EG+ ++DY + WIG +
Sbjct: 50  MAASPGFDKVSEKSEKGKGLTSNSVRDWWWKQDNEVAGLT-QVGEGKKVKDYVMDWIGRE 226


>BE204783 weakly similar to GP|18157659|gb cytochrome P450 {Euphorbia
           lagascae}, partial (34%)
          Length = 599

 Score = 28.1 bits (61), Expect = 8.2
 Identities = 11/35 (31%), Positives = 22/35 (62%)
 Frame = +2

Query: 388 FGDLINLPQVQVGNQEEARFSFLKGGYVEDFENQE 422
           F +++  P++    QEE R +F+  GYV++ + +E
Sbjct: 23  FSEMLKNPRILKRAQEEVRHAFVSRGYVDEKDLEE 127


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.321    0.137    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,838,069
Number of Sequences: 36976
Number of extensions: 197048
Number of successful extensions: 820
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 815
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 819
length of query: 500
length of database: 9,014,727
effective HSP length: 100
effective length of query: 400
effective length of database: 5,317,127
effective search space: 2126850800
effective search space used: 2126850800
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)


Medicago: description of AC127429.2