BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127428.6 - phase: 0 /pseudo
(322 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC81139 similar to PIR|D86427|D86427 hypothetical protein AAG309... 417 e-117
TC91367 similar to GP|9294602|dbj|BAB02903.1 hydrolase-like prot... 83 1e-16
TC90841 similar to GP|9758283|dbj|BAB08807.1 gene_id:MLE2.5~simi... 32 0.25
TC92221 similar to GP|9758283|dbj|BAB08807.1 gene_id:MLE2.5~simi... 29 2.1
TC88581 similar to PIR|A84634|A84634 hypothetical protein At2g24... 28 3.6
TC92553 similar to PIR|T39903|T39903 serine-rich protein - fissi... 28 3.6
TC79517 similar to PIR|T08874|T08874 calcium-dependent protein k... 28 4.8
TC93077 weakly similar to GP|19920182|gb|AAM08614.1 Putative Glu... 28 4.8
BG447781 weakly similar to PIR|F86378|F8 protein F21J9.12 [impor... 28 4.8
TC88228 similar to GP|13605916|gb|AAK32943.1 AT4g00830/A_TM018A1... 27 8.1
>TC81139 similar to PIR|D86427|D86427 hypothetical protein AAG30964.1
[imported] - Arabidopsis thaliana, partial (56%)
Length = 741
Score = 417 bits (1072), Expect = e-117
Identities = 199/199 (100%), Positives = 199/199 (100%)
Frame = +3
Query: 1 MATSNGTDDGTPPSESALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNY 60
MATSNGTDDGTPPSESALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNY
Sbjct: 42 MATSNGTDDGTPPSESALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNY 221
Query: 61 RCNTSMLIDYCGSGSNHNYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGL 120
RCNTSMLIDYCGSGSNHNYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGL
Sbjct: 222 RCNTSMLIDYCGSGSNHNYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGL 401
Query: 121 DDVRAVQPFSPTNDIDPTPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWNIIT 180
DDVRAVQPFSPTNDIDPTPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWNIIT
Sbjct: 402 DDVRAVQPFSPTNDIDPTPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWNIIT 581
Query: 181 DDCNQPFFASGLKFTPLPV 199
DDCNQPFFASGLKFTPLPV
Sbjct: 582 DDCNQPFFASGLKFTPLPV 638
>TC91367 similar to GP|9294602|dbj|BAB02903.1 hydrolase-like protein
{Arabidopsis thaliana}, partial (45%)
Length = 656
Score = 83.2 bits (204), Expect = 1e-16
Identities = 49/115 (42%), Positives = 68/115 (58%), Gaps = 1/115 (0%)
Frame = +3
Query: 3 TSNGTDD-GTPPSESALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYR 61
+SNG + + +S +IF+GTG S +P V CL NPS C VC ++ P +KN R
Sbjct: 171 SSNGIGEVDSHVDQSQVIFIGTGTSEGIPRVSCLTNPSTK-CPVCTKAAK-PGDKNR--R 338
Query: 62 CNTSMLIDYCGSGSNHNYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADA 116
NTS+L+ + HN ILID GK F + L+WF I +D++I+TH HADA
Sbjct: 339 LNTSILVRHSNGTGTHN-ILIDAGKFFYHSALQWFPKFGIRTLDAVIITHSHADA 500
>TC90841 similar to GP|9758283|dbj|BAB08807.1 gene_id:MLE2.5~similar to
unknown protein~sp|P54122 {Arabidopsis thaliana},
partial (27%)
Length = 658
Score = 32.3 bits (72), Expect = 0.25
Identities = 27/93 (29%), Positives = 45/93 (48%), Gaps = 13/93 (13%)
Frame = +1
Query: 74 GSNHNYILIDVGKTFRE-------------TVLRWFVHHRIPKIDSIILTHEHADAVLGL 120
G++ YILID G F + T +R + H KI+++++TH H D + L
Sbjct: 382 GNHDRYILIDAGIMFPDYDDLGVQKIIPDTTFIRKWSH----KIEALVITHGHEDHIGAL 549
Query: 121 DDVRAVQPFSPTNDIDPTPIYLSQHSMDSIEEK 153
V P +N TPI+ S +M+ I+++
Sbjct: 550 P---WVIPALDSN----TPIFASSFTMELIKKR 627
>TC92221 similar to GP|9758283|dbj|BAB08807.1 gene_id:MLE2.5~similar to
unknown protein~sp|P54122 {Arabidopsis thaliana},
partial (37%)
Length = 956
Score = 29.3 bits (64), Expect = 2.1
Identities = 24/93 (25%), Positives = 42/93 (44%), Gaps = 13/93 (13%)
Frame = +3
Query: 74 GSNHNYILIDVGKTFR-------------ETVLRWFVHHRIPKIDSIILTHEHADAVLGL 120
G++ YIL+D G F T ++ + H KI+++++TH H D + L
Sbjct: 444 GNHDRYILVDAGVMFPGDDELGVQKIIPDTTFIKKWSH----KIEAVVITHGHEDHIGAL 611
Query: 121 DDVRAVQPFSPTNDIDPTPIYLSQHSMDSIEEK 153
V P D TP++ S +M+ I ++
Sbjct: 612 PWV------IPMLD-SQTPVFASSFTMELIRKR 689
>TC88581 similar to PIR|A84634|A84634 hypothetical protein At2g24220
[imported] - Arabidopsis thaliana, partial (94%)
Length = 1367
Score = 28.5 bits (62), Expect = 3.6
Identities = 21/85 (24%), Positives = 39/85 (45%)
Frame = +1
Query: 171 VAQMAWNIITDDCNQPFFASGLKFTPLPVMHGEDYICLGFLFGEKSRVAYISDVSRIPAS 230
VA + W +I +F + TPL ++ Y+ LGFL + Y + +PAS
Sbjct: 319 VAVVGWPLIALILLPTYFVTKTVPTPLSLILFLSYVVLGFL-SAADNLMYAYAYAYLPAS 495
Query: 231 TEYVISKSGAGQLDLLILDSLYRFL 255
T +++ S L+ +L+ ++
Sbjct: 496 TAALVASSS------LVFSALFGYI 552
>TC92553 similar to PIR|T39903|T39903 serine-rich protein - fission yeast
(Schizosaccharomyces pombe), partial (6%)
Length = 627
Score = 28.5 bits (62), Expect = 3.6
Identities = 18/53 (33%), Positives = 21/53 (38%)
Frame = +2
Query: 40 DPPCSVCAQSLSIPPEKNPNYRCNTSMLIDYCGSGSNHNYILIDVGKTFRETV 92
DP QSL+IP K+P C S HN L D K FR +
Sbjct: 161 DPKQITALQSLNIPTSKDP------------CTQPSYHNATLCDSSKPFRHLI 283
>TC79517 similar to PIR|T08874|T08874 calcium-dependent protein kinase (EC
2.7.1.-) gamma - soybean, partial (80%)
Length = 1930
Score = 28.1 bits (61), Expect = 4.8
Identities = 24/83 (28%), Positives = 32/83 (37%), Gaps = 25/83 (30%)
Frame = -3
Query: 2 ATSNGTDDGTPPS---------ESALIFLGTGCSSMVPNVLCLIN--------------- 37
A S G + PPS E+ + F G+ S ++ L L +
Sbjct: 1451 ALSIGLSEAFPPSLSQG*SRT*EAVIRFFGSCISILLTRSLALFDILGHG*LSKSI*PCN 1272
Query: 38 -PSDPPCSVCAQSLSIPPEKNPN 59
SD PCSVCAQ+ P K N
Sbjct: 1271 MASDIPCSVCAQNGGTPQSKMCN 1203
>TC93077 weakly similar to GP|19920182|gb|AAM08614.1 Putative Glucan 1
3-beta-glucosidase precursor {Oryza sativa (japonica
cultivar-group)}, partial (29%)
Length = 833
Score = 28.1 bits (61), Expect = 4.8
Identities = 13/45 (28%), Positives = 23/45 (50%)
Frame = -1
Query: 21 LGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYRCNTS 65
+G+G S P + PCS C SL +PP + +++ +T+
Sbjct: 218 IGSGWSVYAPR-------RNQPCSTCLWSLGLPPGERGSFKSSTA 105
>BG447781 weakly similar to PIR|F86378|F8 protein F21J9.12 [imported] -
Arabidopsis thaliana, partial (9%)
Length = 519
Score = 28.1 bits (61), Expect = 4.8
Identities = 21/72 (29%), Positives = 28/72 (38%)
Frame = -3
Query: 186 PFFASGLKFTPLPVMHGEDYICLGFLFGEKSRVAYISDVSRIPASTEYVISKSGAGQLDL 245
PFF + ED CL LF SRV S +S ++++S+ L L
Sbjct: 385 PFFPANSSMYEFNFKLSEDISCLAILFA-SSRVEISSCAFNNASSIDFIVSEEPTNLLAL 209
Query: 246 LILDSLYRFLSF 257
S F SF
Sbjct: 208 FAASSCSFFNSF 173
>TC88228 similar to GP|13605916|gb|AAK32943.1 AT4g00830/A_TM018A10_14
{Arabidopsis thaliana}, partial (67%)
Length = 1712
Score = 27.3 bits (59), Expect = 8.1
Identities = 18/60 (30%), Positives = 27/60 (45%), Gaps = 4/60 (6%)
Frame = -1
Query: 25 CSSMVPNVLCLINPSDPPCSVCAQS----LSIPPEKNPNYRCNTSMLIDYCGSGSNHNYI 80
CSS +P+ PS P S+C+ + SI + + R + S D GSG H +
Sbjct: 302 CSSTLPSAFSSSPPSTPSSSICSSTSSSISSI*FSSSRSTRSSISAPSDMIGSGICHKQV 123
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.332 0.145 0.468
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,596,958
Number of Sequences: 36976
Number of extensions: 189648
Number of successful extensions: 1183
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1181
length of query: 322
length of database: 9,014,727
effective HSP length: 96
effective length of query: 226
effective length of database: 5,465,031
effective search space: 1235097006
effective search space used: 1235097006
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 58 (26.9 bits)
Medicago: description of AC127428.6