BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127169.10 + phase: 1 /pseudo/partial
(367 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86032 similar to PIR|T07612|T07612 cellulase (EC 3.2.1.4) Cel3... 57 1e-08
TC88778 similar to GP|1039431|gb|AAC78504.1| cellulase {Phaseolu... 36 0.001
TC89276 weakly similar to GP|21740771|emb|CAD41248. OSJNBa0067K0... 38 0.005
TC77203 similar to GP|22208353|emb|CAC94006. endo-beta-1 4-gluca... 32 0.30
TC89088 homologue to PIR|H85135|H85135 hypothetical protein AT4g... 25 1.4
TC86381 similar to PIR|E96786|E96786 protein F10A5.13 [imported]... 30 1.5
TC81637 homologue to PIR|JC7226|JC7226 endo-1 3(4)-beta-glucanas... 30 1.9
BE205176 similar to GP|20530716|gb GAGA-binding protein {Glycine... 29 2.5
TC76365 homologue to GP|456568|gb|AAA64427.1|| ubiquitin conjuga... 28 4.3
TC77786 28 4.3
TC84536 similar to GP|18256903|gb|AAH21873.1 Similar to nucleola... 28 7.4
TC76657 homologue to SP|P16059|PSBP_PEA Oxygen-evolving enhancer... 27 9.6
TC92703 similar to PIR|T04993|T04993 probable aspartate--tRNA li... 27 9.6
>TC86032 similar to PIR|T07612|T07612 cellulase (EC 3.2.1.4) Cel3
membrane-anchored - tomato, complete
Length = 2536
Score = 57.0 bits (136), Expect = 1e-08
Identities = 27/63 (42%), Positives = 38/63 (59%), Gaps = 11/63 (17%)
Frame = +2
Query: 52 DHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGSE-----------KPEDMEYPR 100
+H +ELIKWGTDY L TF+S+A I + A+VG + G + +PED++Y R
Sbjct: 803 NHVKELIKWGTDYFLKTFNSTADTITTLAAQVGSGVTGEDSTTPNDHYCWMRPEDIDYDR 982
Query: 101 PTT 103
P T
Sbjct: 983 PVT 991
Score = 48.1 bits (113), Expect = 5e-06
Identities = 26/57 (45%), Positives = 36/57 (62%)
Frame = +2
Query: 311 YDLRNNNSYTEPTLAGNAGLVAALISLTSTSTIGSGFDTDHTIFNQTRQYRPQNLPP 367
+D+R N +YTEPTLAGNAGLVAAL++L+ +I +T+F+ P PP
Sbjct: 1886 HDVRKNYNYTEPTLAGNAGLVAALVALSGDKSIPI---DKNTLFSAVPPMFPTPPPP 2047
Score = 45.8 bits (107), Expect(2) = 3e-06
Identities = 31/88 (35%), Positives = 46/88 (52%)
Frame = +1
Query: 219 WLPLRGNFEYVP*IY*PYHVLLTKIVVAIIKICRGIYTIEPWIPKFSPICC*CFIYGFSF 278
W+ + N + +P HVLL + + R + +I+PW SPICC C ++G+
Sbjct: 1393 WISI*RNSKDLPQSDQHRHVLLPTCFHKL*QNQRKLDSIKPW*ASASPICCQCSLFGYPI 1572
Query: 279 C*LYESPRCSWMVL*S*LLFNICSKGIC 306
*L SWMVL +*LLFN + +C
Sbjct: 1573 Q*LP*CR*YSWMVLWT*LLFN*KTP*VC 1656
Score = 22.7 bits (47), Expect(2) = 3e-06
Identities = 8/13 (61%), Positives = 13/13 (99%)
Frame = +2
Query: 205 LLLTRVRMFLNPG 217
+LL+R+R+FL+PG
Sbjct: 1358 VLLSRLRLFLSPG 1396
>TC88778 similar to GP|1039431|gb|AAC78504.1| cellulase {Phaseolus
vulgaris}, partial (49%)
Length = 781
Score = 35.8 bits (81), Expect(2) = 0.001
Identities = 19/65 (29%), Positives = 34/65 (52%)
Frame = +1
Query: 46 NTVNRYDHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGSEKPEDMEYPRPTTTI 105
++VN+ + R I+WGT+++L + S + Q+ + E+PEDM+ PR I
Sbjct: 160 SSVNQIGYLRRAIRWGTNFILQSHTSPITLFTQV-GEGNADHNCWERPEDMDTPRTLYKI 336
Query: 106 *SEGP 110
+ P
Sbjct: 337 DANSP 351
Score = 23.5 bits (49), Expect(2) = 0.001
Identities = 24/86 (27%), Positives = 38/86 (43%), Gaps = 22/86 (25%)
Frame = +2
Query: 114 LLIPRKLSRELKLSLH---LQETLVKGHPTV------VDNLYI--------EPNIITH-- 154
+LIP +L +L+L LH LQ +L K P + + NL + ++ H
Sbjct: 338 MLIPLELRLQLRLLLHFQLLQLSLRKKIPNIRRSY*DIQNLCLILLTSIEAPTQVLVHST 517
Query: 155 ---LATLMSTCGVVFGYFWPLVTTPT 177
L T M+ G++ GY P+ T
Sbjct: 518 ARTLVTKMNYYGLLLGYTRPVEKAST 595
>TC89276 weakly similar to GP|21740771|emb|CAD41248. OSJNBa0067K08.12 {Oryza
sativa}, partial (15%)
Length = 1389
Score = 38.1 bits (87), Expect = 0.005
Identities = 17/30 (56%), Positives = 23/30 (76%)
Frame = +1
Query: 312 DLRNNNSYTEPTLAGNAGLVAALISLTSTS 341
D R+N +TEPT++ NAGLV+ALI+L S
Sbjct: 1084 DQRSNKRFTEPTISSNAGLVSALIALQDPS 1173
Score = 36.2 bits (82), Expect = 0.021
Identities = 22/74 (29%), Positives = 34/74 (45%), Gaps = 9/74 (12%)
Frame = +2
Query: 57 LIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGS---------EKPEDMEYPRPTTTI*S 107
+ +WGTDYL F ++++VG ++ + ++PEDM Y RP +
Sbjct: 2 IFRWGTDYLHKVFIPPNGSNLTLYSQVGSTISTNHEPNDISCWQRPEDMSYGRPVSVCDG 181
Query: 108 EGPDLALLIPRKLS 121
DLA I LS
Sbjct: 182 SATDLAGEIVAALS 223
>TC77203 similar to GP|22208353|emb|CAC94006. endo-beta-1 4-glucanase
{Fragaria x ananassa}, partial (92%)
Length = 2243
Score = 32.3 bits (72), Expect = 0.30
Identities = 23/66 (34%), Positives = 32/66 (47%), Gaps = 5/66 (7%)
Frame = +1
Query: 53 HARELIKWGTDYLLLTFDSSASEINQIHAKVGG---SLKGSEKPEDMEYPRPTTTI*SEG 109
HA E +KWGTDY F + + N ++ +VG K ++PEDM R +
Sbjct: 499 HAMEAVKWGTDY----FIKAHPQPNLLYGEVGDGNTDHKCWQRPEDMTTDRRAYKVDPSN 666
Query: 110 P--DLA 113
P DLA
Sbjct: 667 PGSDLA 684
>TC89088 homologue to PIR|H85135|H85135 hypothetical protein AT4g12650
[imported] - Arabidopsis thaliana, partial (53%)
Length = 1275
Score = 25.4 bits (54), Expect(2) = 1.4
Identities = 16/54 (29%), Positives = 27/54 (49%), Gaps = 2/54 (3%)
Frame = +2
Query: 307 NFSVYDLRNNNSYTEPTLAGNAGLVAALISLTSTSTIG--SGFDTDHTIFNQTR 358
N+ V+DL++ + TL L+ A+ + ST TIG F H +F+ +
Sbjct: 674 NYLVFDLQSLSGPVSATLYLGYSLLMAIAIMLSTGTIGFLMSFYFVHYLFSSVK 835
Score = 23.1 bits (48), Expect(2) = 1.4
Identities = 10/23 (43%), Positives = 12/23 (51%)
Frame = +3
Query: 267 ICC*CFIYGFSFC*LYESPRCSW 289
I C + F +C*L PRC W
Sbjct: 504 ITCLVSCWLFFYC*LLFVPRCRW 572
>TC86381 similar to PIR|E96786|E96786 protein F10A5.13 [imported] -
Arabidopsis thaliana, partial (92%)
Length = 2291
Score = 30.0 bits (66), Expect = 1.5
Identities = 19/57 (33%), Positives = 29/57 (50%), Gaps = 4/57 (7%)
Frame = +2
Query: 307 NFSVYDLRNNNSYTEPTLAGNAGLVAALISLTSTSTIGSGFD----TDHTIFNQTRQ 359
N + D RNN+ EP+ +A +V L SL +TS+ F +IFN T++
Sbjct: 1880 NETYIDSRNNSMQGEPSTYNSAVIVGLLSSLVTTSSALQSFS*TIVCSFSIFNSTQK 2050
>TC81637 homologue to PIR|JC7226|JC7226 endo-1 3(4)-beta-glucanase (EC
3.2.1.6) - garden pea, partial (82%)
Length = 1251
Score = 29.6 bits (65), Expect = 1.9
Identities = 19/52 (36%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Frame = +1
Query: 62 TDYLLLTFDSSASEINQIHAKVGGSLKGS---EKPEDMEYPRPTTTI*SEGP 110
TDYLL + + N I+ +VG + K E+PEDM+ PR I + P
Sbjct: 286 TDYLL----KATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKIDANAP 429
>BE205176 similar to GP|20530716|gb GAGA-binding protein {Glycine max},
partial (43%)
Length = 643
Score = 29.3 bits (64), Expect = 2.5
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = -1
Query: 186 LLCLRMPKHLIRLPILISYLLLTRVRMFLN 215
LLCLR +HL LP+L S + L R FL+
Sbjct: 622 LLCLRRLRHLRSLPLLTSSIHLLNHRYFLH 533
>TC76365 homologue to GP|456568|gb|AAA64427.1|| ubiquitin conjugating enzyme
{Pisum sativum}, complete
Length = 856
Score = 28.5 bits (62), Expect = 4.3
Identities = 9/28 (32%), Positives = 20/28 (71%)
Frame = +3
Query: 177 TFPWQWQPILLCLRMPKHLIRLPILISY 204
T PWQW+ I++ + +HL+++ L+++
Sbjct: 648 TQPWQWELIIIL*KFLRHLVQIFCLLTF 731
>TC77786
Length = 1094
Score = 28.5 bits (62), Expect = 4.3
Identities = 10/17 (58%), Positives = 13/17 (75%)
Frame = +3
Query: 164 VVFGYFWPLVTTPTFPW 180
V+F Y+ P+VTTP PW
Sbjct: 630 VLFHYYLPVVTTPYLPW 680
>TC84536 similar to GP|18256903|gb|AAH21873.1 Similar to nucleolar protein
family A member 1 (H/ACA small nucleolar RNPs) {Mus
musculus}, partial (13%)
Length = 698
Score = 27.7 bits (60), Expect = 7.4
Identities = 23/68 (33%), Positives = 34/68 (49%), Gaps = 4/68 (5%)
Frame = +1
Query: 184 PILLCLRMPKHLIRLPILISYLLLTR----VRMFLNPG*WLPLRGNFEYVP*IY*PYHVL 239
P+LL +++P L+R ILI L TR V + LNP W + P I+ H L
Sbjct: 232 PLLLIIQLPLILLRFNILIIKLNPTRFILLVLILLNPTFW--------FNPTIFRSGHHL 387
Query: 240 LTKIVVAI 247
T++ V +
Sbjct: 388 NTEVGVVV 411
>TC76657 homologue to SP|P16059|PSBP_PEA Oxygen-evolving enhancer protein 2
chloroplast precursor (OEE2), complete
Length = 1089
Score = 27.3 bits (59), Expect = 9.6
Identities = 11/26 (42%), Positives = 17/26 (65%), Gaps = 1/26 (3%)
Frame = +3
Query: 265 SPICC*CFIYGFSF-C*LYESPRCSW 289
+P CC C++ GF+ C L+ S +C W
Sbjct: 231 TPWCCCCWLKGFTC*CCLWRSCQCLW 308
>TC92703 similar to PIR|T04993|T04993 probable aspartate--tRNA ligase (EC
6.1.1.12) T16L1.250 - Arabidopsis thaliana, partial
(30%)
Length = 694
Score = 27.3 bits (59), Expect = 9.6
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +3
Query: 52 DHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGSEKPEDM 96
DHAR I W TD+ + ++ S + +H + PEDM
Sbjct: 459 DHARHSILWITDFPMFEWNDSEQRLEALHHPF-----TAPNPEDM 578
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.343 0.153 0.541
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,518,264
Number of Sequences: 36976
Number of extensions: 239872
Number of successful extensions: 2209
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 2187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2207
length of query: 367
length of database: 9,014,727
effective HSP length: 97
effective length of query: 270
effective length of database: 5,428,055
effective search space: 1465574850
effective search space used: 1465574850
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.5 bits)
S2: 59 (27.3 bits)
Medicago: description of AC127169.10