BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126782.8 + phase: 0 /pseudo
(250 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC76434 similar to GP|7715089|gb|AAF67863.1| cyclopropyl isomera... 184 4e-64
TC76381 similar to GP|7715089|gb|AAF67863.1| cyclopropyl isomera... 217 4e-57
BE205170 similar to GP|7715089|gb|A cyclopropyl isomerase {Arabi... 78 5e-21
AJ501097 31 0.51
TC79325 29 1.9
TC82355 similar to SP|P82538|TL26_ARATH Thylakoid lumenal 25.6 k... 28 2.5
BG451854 similar to SP|P82538|TL26 Thylakoid lumenal 25.6 kDa pr... 28 2.5
TC89767 similar to GP|9759165|dbj|BAB09721.1 gene_id:MEE6.26~unk... 28 4.3
TC82289 similar to GP|12382007|dbj|BAB21276. putative ABC transp... 27 5.7
TC91491 similar to PIR|A71213|A71213 alanine--tRNA ligase trunca... 27 7.4
TC86961 similar to GP|1742951|emb|CAA70946.1 Ca2+-ATPase {Arabid... 27 7.4
TC85810 similar to GP|13516922|dbj|BAB40340. 12-oxophytodienoic ... 27 7.4
TC89007 similar to PIR|T01617|T01617 probable protein kinase [im... 27 7.4
BG454580 27 9.7
TC77500 weakly similar to SP|P21747|EA92_VICFA Embryonic abundan... 27 9.7
BQ122819 similar to PIR|C84748|C84 hypothetical protein At2g3368... 27 9.7
BQ135562 27 9.7
TC85625 SP|Q40345|IDHP_MEDSA Isocitrate dehydrogenase [NADP] ch... 27 9.7
CB894590 similar to GP|6143903|gb|A 12-oxophytodienoate reductas... 27 9.7
TC79991 similar to GP|10177307|dbj|BAB10568. contains similarity... 27 9.7
>TC76434 similar to GP|7715089|gb|AAF67863.1| cyclopropyl isomerase
{Arabidopsis thaliana}, partial (49%)
Length = 516
Score = 184 bits (467), Expect(2) = 4e-64
Identities = 87/91 (95%), Positives = 88/91 (96%)
Frame = +1
Query: 21 SSSSASPTPSLWFAPNLSKRWGELFFLLYTPFWLSLSLGIVVPYKLYENFTELEYLLLGL 80
S ASPTPSLWFAPNLSKRWGELFFLLYTPFWLSLSLGIVVPYKLYENFTELEYLLLGL
Sbjct: 73 SEMGASPTPSLWFAPNLSKRWGELFFLLYTPFWLSLSLGIVVPYKLYENFTELEYLLLGL 252
Query: 81 ISAVPAFLIPMLFVGKADKGISWQDRYWVKS 111
ISAVPAFLIPMLFVGKADKGISWQDRYWVK+
Sbjct: 253 ISAVPAFLIPMLFVGKADKGISWQDRYWVKA 345
Score = 77.8 bits (190), Expect(2) = 4e-64
Identities = 35/36 (97%), Positives = 36/36 (99%)
Frame = +3
Query: 110 KSMDNNLQLCWELFLDSLLFHSSGCILYLSFMENEQ 145
+SMDNNLQLCWELFLDSLLFHSSGCILYLSFMENEQ
Sbjct: 345 QSMDNNLQLCWELFLDSLLFHSSGCILYLSFMENEQ 452
>TC76381 similar to GP|7715089|gb|AAF67863.1| cyclopropyl isomerase
{Arabidopsis thaliana}, partial (93%)
Length = 1374
Score = 217 bits (552), Expect = 4e-57
Identities = 104/105 (99%), Positives = 105/105 (99%)
Frame = +1
Query: 146 SYLETLAISNFPYYEFVDRESMYKVGSLFYAIYFIVSFPMFLRIDEKPGDKWDLPRVAVD 205
+YLETLAISNFPYYEFVDRESMYKVGSLFYAIYFIVSFPMFLRIDEKPGDKWDLPRVAVD
Sbjct: 724 AYLETLAISNFPYYEFVDRESMYKVGSLFYAIYFIVSFPMFLRIDEKPGDKWDLPRVAVD 903
Query: 206 ALGAAMLVTIILDLWRIFLGPIVPVSEAKQCSPTGLPWFSTHANL 250
ALGAAMLVTIILDLWRIFLGPIVPVSEAKQCSPTGLPWFSTHANL
Sbjct: 904 ALGAAMLVTIILDLWRIFLGPIVPVSEAKQCSPTGLPWFSTHANL 1038
Score = 183 bits (464), Expect = 6e-47
Identities = 86/87 (98%), Positives = 87/87 (99%)
Frame = +1
Query: 25 ASPTPSLWFAPNLSKRWGELFFLLYTPFWLSLSLGIVVPYKLYENFTELEYLLLGLISAV 84
ASPTPSLWFAPNLSKRWGELFFLLYTPFWLSLSLGIVVPYKLYENFTELEYLLLGLISAV
Sbjct: 193 ASPTPSLWFAPNLSKRWGELFFLLYTPFWLSLSLGIVVPYKLYENFTELEYLLLGLISAV 372
Query: 85 PAFLIPMLFVGKADKGISWQDRYWVKS 111
PAFLIPMLFVGKADKGISWQDRYWVK+
Sbjct: 373 PAFLIPMLFVGKADKGISWQDRYWVKA 453
Score = 99.0 bits (245), Expect = 2e-21
Identities = 72/159 (45%), Positives = 81/159 (50%), Gaps = 14/159 (8%)
Frame = +3
Query: 1 MVSSILFSKSLFCVSVLEISSSSSASPTPSLWFAPNLSKRWGELF------FLLYTPFWL 54
MVSSILFSKSLFCVSVLEISSSSS L + G FL ++ W
Sbjct: 120 MVSSILFSKSLFCVSVLEISSSSSGFTNS*LVVCTKSQ*KMGRTLLSSLHSFLAFSFSWH 299
Query: 55 SLSLGIVVPYKLYENFTELEYLLLGLISAVPAF--------LIPMLFVGKADKGISWQDR 106
SL + + FT L A ++ +GK
Sbjct: 300 CCSLQALREFY*IGVFTSWVNLSSSCFPHSHAVRW*G**RHILAGPLLGKG--------- 452
Query: 107 YWVKSMDNNLQLCWELFLDSLLFHSSGCILYLSFMENEQ 145
+SMDNNLQLCWELFLDSLLFHSSGCILYLSFMENEQ
Sbjct: 453 ---QSMDNNLQLCWELFLDSLLFHSSGCILYLSFMENEQ 560
>BE205170 similar to GP|7715089|gb|A cyclopropyl isomerase {Arabidopsis
thaliana}, partial (21%)
Length = 699
Score = 77.8 bits (190), Expect(2) = 5e-21
Identities = 35/36 (97%), Positives = 36/36 (99%)
Frame = +1
Query: 110 KSMDNNLQLCWELFLDSLLFHSSGCILYLSFMENEQ 145
+SMDNNLQLCWELFLDSLLFHSSGCILYLSFMENEQ
Sbjct: 571 QSMDNNLQLCWELFLDSLLFHSSGCILYLSFMENEQ 678
Score = 40.0 bits (92), Expect(2) = 5e-21
Identities = 15/17 (88%), Positives = 17/17 (99%)
Frame = +2
Query: 95 GKADKGISWQDRYWVKS 111
GK+DKGISWQDRYWVK+
Sbjct: 521 GKSDKGISWQDRYWVKA 571
>AJ501097
Length = 539
Score = 30.8 bits (68), Expect = 0.51
Identities = 15/49 (30%), Positives = 27/49 (54%)
Frame = +1
Query: 137 YLSFMENEQSYLETLAISNFPYYEFVDRESMYKVGSLFYAIYFIVSFPM 185
Y S E ++I+NF + +F+ S++ + SLF++ YF +F M
Sbjct: 391 YYGIFNGCSSITEEISINNFYFSKFILPFSIFYIPSLFFSHYFKYNFRM 537
>TC79325
Length = 1854
Score = 28.9 bits (63), Expect = 1.9
Identities = 12/33 (36%), Positives = 22/33 (66%)
Frame = +3
Query: 1 MVSSILFSKSLFCVSVLEISSSSSASPTPSLWF 33
+++ +FS +F +S SSS++ +P PSL+F
Sbjct: 63 LLTFFIFSYLIFFISFFSSSSSATTTPPPSLFF 161
>TC82355 similar to SP|P82538|TL26_ARATH Thylakoid lumenal 25.6 kDa protein
chloroplast precursor. [Mouse-ear cress] {Arabidopsis
thaliana}, partial (37%)
Length = 739
Score = 28.5 bits (62), Expect = 2.5
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +2
Query: 63 PYKLYENFTELEYLLLGLISAVPAFLIPMLFV 94
P++L+ +FTEL + L L S V ++ +LFV
Sbjct: 128 PFRLHLHFTELSFKTLSLRSMVVVVVVVVLFV 223
>BG451854 similar to SP|P82538|TL26 Thylakoid lumenal 25.6 kDa protein
chloroplast precursor. [Mouse-ear cress] {Arabidopsis
thaliana}, partial (49%)
Length = 636
Score = 28.5 bits (62), Expect = 2.5
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +1
Query: 63 PYKLYENFTELEYLLLGLISAVPAFLIPMLFV 94
P++L+ +FTEL + L L S V ++ +LFV
Sbjct: 73 PFRLHLHFTELSFKTLSLRSMVVVVVVVVLFV 168
>TC89767 similar to GP|9759165|dbj|BAB09721.1 gene_id:MEE6.26~unknown protein
{Arabidopsis thaliana}, partial (46%)
Length = 1587
Score = 27.7 bits (60), Expect = 4.3
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = -1
Query: 4 SILFSKSLFCVSVLEISSSSSASPT 28
SI FS SL+C S E+ SSS P+
Sbjct: 1113 SICFSSSLWCTSRFEVLSSSQTLPS 1039
>TC82289 similar to GP|12382007|dbj|BAB21276. putative ABC transporter
protein {Oryza sativa (japonica cultivar-group)},
partial (25%)
Length = 1108
Score = 27.3 bits (59), Expect = 5.7
Identities = 12/17 (70%), Positives = 14/17 (81%)
Frame = +2
Query: 3 SSILFSKSLFCVSVLEI 19
SS LF +SLFC +VLEI
Sbjct: 665 SSTLFERSLFCFTVLEI 715
>TC91491 similar to PIR|A71213|A71213 alanine--tRNA ligase truncated homolog
PH1969 - Pyrococcus horikoshii, partial (4%)
Length = 684
Score = 26.9 bits (58), Expect = 7.4
Identities = 18/51 (35%), Positives = 24/51 (46%)
Frame = +1
Query: 41 WGELFFLLYTPFWLSLSLGIVVPYKLYENFTELEYLLLGLISAVPAFLIPM 91
W E F L+Y LS +V ++ L LG+I AVPA +PM
Sbjct: 172 WEEKFLLIYYCMMKLLSFVVVFFLIMFLRKAHLALFRLGIILAVPA-AVPM 321
>TC86961 similar to GP|1742951|emb|CAA70946.1 Ca2+-ATPase {Arabidopsis
thaliana}, partial (77%)
Length = 1488
Score = 26.9 bits (58), Expect = 7.4
Identities = 28/85 (32%), Positives = 37/85 (42%), Gaps = 2/85 (2%)
Frame = +1
Query: 18 EISSSSSASPTPSLWFAPNLSKRWGELFFLLYTPFWLSLSLGIVVPYKLYENFTELEYLL 77
E S + P + W +S +G F +LY PF L+ GI VP E L
Sbjct: 784 EDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPF-LAKVFGI-VPLSFNE-------WL 936
Query: 78 LGLISAVPAFLIP--MLFVGKADKG 100
L L A+P LI + FVG+ G
Sbjct: 937 LVLAVALPVILIDEVLKFVGRCTSG 1011
>TC85810 similar to GP|13516922|dbj|BAB40340. 12-oxophytodienoic acid 10
11-reductase {Pisum sativum}, partial (72%)
Length = 929
Score = 26.9 bits (58), Expect = 7.4
Identities = 15/34 (44%), Positives = 18/34 (52%)
Frame = +2
Query: 99 KGISWQDRYWVKSMDNNLQLCWELFLDSLLFHSS 132
KGI Y +KSM L W LF+ +L HSS
Sbjct: 254 KGIQTHQVYGLKSMSRRGNLLWMLFMPKVL-HSS 352
>TC89007 similar to PIR|T01617|T01617 probable protein kinase [imported] -
Arabidopsis thaliana, partial (24%)
Length = 720
Score = 26.9 bits (58), Expect = 7.4
Identities = 24/77 (31%), Positives = 38/77 (49%), Gaps = 4/77 (5%)
Frame = -3
Query: 111 SMDNNLQLCWELFLDSLLFHSSGCILYLSFMENEQSYLETLAISNFPYYEFVDRESMYKV 170
+M N LC +LFL S + SS L+ +F Q L L+I+ F + F E++ +
Sbjct: 571 NMTNCSNLCQKLFLFSFIL*SSC*SLHSNFFTIFQCSLVNLSITTFSNHIF-*VEAIGSI 395
Query: 171 GSLF----YAIYFIVSF 183
LF + +F+ SF
Sbjct: 394 KQLFIRETFPCWFLTSF 344
>BG454580
Length = 651
Score = 26.6 bits (57), Expect = 9.7
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = +3
Query: 111 SMDNNLQLCWELFLDSLLFHSSGCILYLSF 140
S D NL + +FLD L S GC L+L F
Sbjct: 63 SSDFNLFIMQFIFLDFYLAFSIGCFLFLIF 152
>TC77500 weakly similar to SP|P21747|EA92_VICFA Embryonic abundant protein
USP92 precursor. [Broad bean] {Vicia faba}, partial
(40%)
Length = 1128
Score = 26.6 bits (57), Expect = 9.7
Identities = 21/75 (28%), Positives = 39/75 (52%)
Frame = +1
Query: 46 FLLYTPFWLSLSLGIVVPYKLYENFTELEYLLLGLISAVPAFLIPMLFVGKADKGISWQD 105
F LY+ + L SL +++ K +F ++ Y+L +A +FLI LF ++ + +
Sbjct: 55 F*LYSLWLLRESLHLILTLKANYSFWKMTYILARK*AASKSFLIFNLF----EQSVGCRS 222
Query: 106 RYWVKSMDNNLQLCW 120
K + NNL++ W
Sbjct: 223 ----KKLLNNLEM*W 255
>BQ122819 similar to PIR|C84748|C84 hypothetical protein At2g33680 [imported]
- Arabidopsis thaliana, partial (15%)
Length = 771
Score = 26.6 bits (57), Expect = 9.7
Identities = 21/76 (27%), Positives = 36/76 (46%), Gaps = 4/76 (5%)
Frame = -2
Query: 47 LLYTPFWLSLSLGIVV----PYKLYENFTELEYLLLGLISAVPAFLIPMLFVGKADKGIS 102
+LY+P WLSLS G + P KLY + + + L++ P +S
Sbjct: 293 VLYSPLWLSLSSGHALHFPYPLKLYIYLITI-HKQMTLVTLAPL--------------VS 159
Query: 103 WQDRYWVKSMDNNLQL 118
Q ++ V+S D++ +L
Sbjct: 158 LQHKHLVRSSDDSYKL 111
>BQ135562
Length = 870
Score = 26.6 bits (57), Expect = 9.7
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +3
Query: 153 ISNFPYYEFVDRESMYKVGSLFYAIYFIVSFPM 185
ISNFP+ F YK+ FY IY+ +P+
Sbjct: 588 ISNFPF--FSHPSPCYKIYYKFYHIYYFYLYPI 680
>TC85625 SP|Q40345|IDHP_MEDSA Isocitrate dehydrogenase [NADP] chloroplast
precursor (EC 1.1.1.42) (Oxalosuccinate decarboxylase),
partial (97%)
Length = 1733
Score = 26.6 bits (57), Expect = 9.7
Identities = 16/46 (34%), Positives = 25/46 (53%), Gaps = 1/46 (2%)
Frame = -3
Query: 20 SSSSSASPTPSLWFAPNL-SKRWGELFFLLYTPFWLSLSLGIVVPY 64
S S + S + +LW P + +K LF L+ PFW +L + VP+
Sbjct: 1167 SKSVALSSSFALWARPLVQAKMDAMLFVLVSPPFW*TLK*RVTVPW 1030
>CB894590 similar to GP|6143903|gb|A 12-oxophytodienoate reductase (OPR2);
15748-17127 {Arabidopsis thaliana}, partial (20%)
Length = 461
Score = 26.6 bits (57), Expect = 9.7
Identities = 15/34 (44%), Positives = 18/34 (52%)
Frame = +1
Query: 99 KGISWQDRYWVKSMDNNLQLCWELFLDSLLFHSS 132
KGI Y +KSM L W LF+ +L HSS
Sbjct: 103 KGIQKHQVYGLKSMSRRGNLLWMLFMPKVL-HSS 201
>TC79991 similar to GP|10177307|dbj|BAB10568. contains similarity to zinc
finger protein~gene_id:MDC12.23 {Arabidopsis thaliana},
partial (8%)
Length = 1023
Score = 26.6 bits (57), Expect = 9.7
Identities = 12/28 (42%), Positives = 18/28 (63%)
Frame = +2
Query: 11 LFCVSVLEISSSSSASPTPSLWFAPNLS 38
L SV ++SSSSS+S + S W ++S
Sbjct: 53 LLSASVCDLSSSSSSSSSSSCWLQKSIS 136
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.141 0.449
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,201,497
Number of Sequences: 36976
Number of extensions: 173189
Number of successful extensions: 1379
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 1366
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1378
length of query: 250
length of database: 9,014,727
effective HSP length: 94
effective length of query: 156
effective length of database: 5,538,983
effective search space: 864081348
effective search space used: 864081348
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC126782.8