BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126782.6 - phase: 1
(422 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78306 homologue to GP|20259085|gb|AAM14258.1 putative arginino... 551 e-166
TC79176 similar to GP|20259085|gb|AAM14258.1 putative argininosu... 418 e-122
TC89116 similar to GP|20259085|gb|AAM14258.1 putative argininosu... 376 e-105
TC78308 homologue to GP|20259085|gb|AAM14258.1 putative arginino... 253 7e-68
TC78307 similar to GP|20259085|gb|AAM14258.1 putative argininosu... 214 7e-56
AA661039 similar to GP|20259085|gb| putative argininosuccinate s... 112 1e-41
AL387742 similar to PIR|T40457|T40 argininosuccinate synthase - ... 159 1e-39
AL387743 weakly similar to PIR|T40457|T404 argininosuccinate syn... 69 3e-12
TC89526 similar to GP|14596139|gb|AAK68797.1 Unknown protein {Ar... 29 3.0
TC85792 homologue to GP|18150857|dbj|BAB83689. mitochondrial pho... 29 3.9
TC78652 similar to PIR|E86270|E86270 hypothetical protein AAF812... 28 5.1
AW584299 similar to GP|20160694|db hypothetical protein~similar ... 28 5.1
AL380183 28 6.7
TC91306 weakly similar to PIR|T06371|T06371 probable UDP-glucuro... 28 8.7
>TC78306 homologue to GP|20259085|gb|AAM14258.1 putative argininosuccinate
synthase {Arabidopsis thaliana}, partial (63%)
Length = 1267
Score = 551 bits (1421), Expect(2) = e-166
Identities = 263/310 (84%), Positives = 294/310 (94%), Gaps = 2/310 (0%)
Frame = +3
Query: 1 IKAVLYKD--VEISETKKVAGLKGKLNKVVLAYSGGLDTSVVVPWLRENYGCDVVCFTAD 58
+KAV + D VE+SE KK +GL+GKLNKVVLAYSGGLDTSV+VPWLRENYGCDVVCFTAD
Sbjct: 249 VKAVSHSDTDVEVSEAKKGSGLRGKLNKVVLAYSGGLDTSVIVPWLRENYGCDVVCFTAD 428
Query: 59 LGQGASELEGLEEKAKASGASQLVVKDLKEEFVRDYVFPCLRAGAIYERKYLLGTAIARP 118
+GQG EL+GLE KAKASGASQLVVKDLKEEFV+DYVFPCLRAGAIYERKYLLGT++ARP
Sbjct: 429 VGQGIKELDGLEAKAKASGASQLVVKDLKEEFVKDYVFPCLRAGAIYERKYLLGTSMARP 608
Query: 119 VIAKAMVDIANEVGADAVSHGCTGKGNDQVRFELSFFALNPKLNVVAPWREWDITGREDA 178
VIAKAMVD+A EVGADAVSHGCTGKGNDQVRFEL+FFALNPKLN+VAPW+EWDITGREDA
Sbjct: 609 VIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELTFFALNPKLNIVAPWKEWDITGREDA 788
Query: 179 IEYAKKHDVPVPVTKKSIYSRDRNLWHITHEGDVLEDPANEPKKDMYMLTVDPEDAPNEP 238
IEYAKKH+VPVPVTKKSIYSRDRNLWH++HEGD+LEDPANEPKKDMYM++VDPEDAP++
Sbjct: 789 IEYAKKHNVPVPVTKKSIYSRDRNLWHLSHEGDILEDPANEPKKDMYMMSVDPEDAPDQA 968
Query: 239 EYVEIGFELGIPVSLNGKRLSPGNLVAELNEIGGRHGIGRVDLVEDRIVGMKSRGVYETP 298
EY+EIG E G+PVSLNGK LSP +L+AELNEIGGRHGIGR+D+VE+R+VGMKSRGVYETP
Sbjct: 969 EYLEIGIESGLPVSLNGKTLSPASLLAELNEIGGRHGIGRIDMVENRLVGMKSRGVYETP 1148
Query: 299 GGTILFTAAR 308
GGTILF AAR
Sbjct: 1149GGTILFAAAR 1178
Score = 52.8 bits (125), Expect(2) = e-166
Identities = 25/28 (89%), Positives = 28/28 (99%)
Frame = +2
Query: 310 LETLTLDRETLQLKDSLALKYAELVYAG 337
LE+LTLDRET+Q+KDSLALKYAELVYAG
Sbjct: 1184 LESLTLDRETIQVKDSLALKYAELVYAG 1267
>TC79176 similar to GP|20259085|gb|AAM14258.1 putative argininosuccinate
synthase {Arabidopsis thaliana}, partial (41%)
Length = 836
Score = 418 bits (1074), Expect(2) = e-122
Identities = 208/210 (99%), Positives = 208/210 (99%)
Frame = +2
Query: 1 IKAVLYKDVEISETKKVAGLKGKLNKVVLAYSGGLDTSVVVPWLRENYGCDVVCFTADLG 60
IKAVLYKDVEISETKKVAGLKGKLNKVVLAYSGGLDTSVVVPWLRENYGCDVVCFTADLG
Sbjct: 167 IKAVLYKDVEISETKKVAGLKGKLNKVVLAYSGGLDTSVVVPWLRENYGCDVVCFTADLG 346
Query: 61 QGASELEGLEEKAKASGASQLVVKDLKEEFVRDYVFPCLRAGAIYERKYLLGTAIARPVI 120
QGASELEGLEEKAKASGASQLVVKDLKEEFVRDYVFPCLRAGAIYERKYLLGTAIARPVI
Sbjct: 347 QGASELEGLEEKAKASGASQLVVKDLKEEFVRDYVFPCLRAGAIYERKYLLGTAIARPVI 526
Query: 121 AKAMVDIANEVGADAVSHGCTGKGNDQVRFELSFFALNPKLNVVAPWREWDITGREDAIE 180
AKAMVDIANEVGADAVSHGCTGKGNDQVRFELSFFALNPKLNVVAPWREWDITGREDAIE
Sbjct: 527 AKAMVDIANEVGADAVSHGCTGKGNDQVRFELSFFALNPKLNVVAPWREWDITGREDAIE 706
Query: 181 YAKKHDVPVPVTKKSIYSRDRNLWHITHEG 210
YAKKHDVPVPVTKKSIYSRDRNLWHIT G
Sbjct: 707 YAKKHDVPVPVTKKSIYSRDRNLWHITS*G 796
Score = 38.9 bits (89), Expect(2) = e-122
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = +3
Query: 208 HEGDVLEDPANEPKKD 223
HEGDVLEDPANEPKKD
Sbjct: 789 HEGDVLEDPANEPKKD 836
>TC89116 similar to GP|20259085|gb|AAM14258.1 putative argininosuccinate
synthase {Arabidopsis thaliana}, partial (37%)
Length = 727
Score = 376 bits (966), Expect = e-105
Identities = 190/190 (100%), Positives = 190/190 (100%)
Frame = +1
Query: 233 DAPNEPEYVEIGFELGIPVSLNGKRLSPGNLVAELNEIGGRHGIGRVDLVEDRIVGMKSR 292
DAPNEPEYVEIGFELGIPVSLNGKRLSPGNLVAELNEIGGRHGIGRVDLVEDRIVGMKSR
Sbjct: 1 DAPNEPEYVEIGFELGIPVSLNGKRLSPGNLVAELNEIGGRHGIGRVDLVEDRIVGMKSR 180
Query: 293 GVYETPGGTILFTAARDLETLTLDRETLQLKDSLALKYAELVYAGRWFDPLRESMDSFME 352
GVYETPGGTILFTAARDLETLTLDRETLQLKDSLALKYAELVYAGRWFDPLRESMDSFME
Sbjct: 181 GVYETPGGTILFTAARDLETLTLDRETLQLKDSLALKYAELVYAGRWFDPLRESMDSFME 360
Query: 353 KISQTTTGSVSLKLYKGSASVTGRKSPYSLYREDISSFESGEIYNHADAVGFIKLYGLPM 412
KISQTTTGSVSLKLYKGSASVTGRKSPYSLYREDISSFESGEIYNHADAVGFIKLYGLPM
Sbjct: 361 KISQTTTGSVSLKLYKGSASVTGRKSPYSLYREDISSFESGEIYNHADAVGFIKLYGLPM 540
Query: 413 RIRAMMEQGI 422
RIRAMMEQGI
Sbjct: 541 RIRAMMEQGI 570
>TC78308 homologue to GP|20259085|gb|AAM14258.1 putative argininosuccinate
synthase {Arabidopsis thaliana}, partial (54%)
Length = 1028
Score = 253 bits (647), Expect = 7e-68
Identities = 137/248 (55%), Positives = 171/248 (68%), Gaps = 11/248 (4%)
Frame = +3
Query: 144 GNDQVRFELSFFALN--PKLNVVAPWREWDITGREDAIEYAKKHDVPVPVTKKSIYSRDR 201
G ++RF+LS +L P + + G EDAIEYAKKH+VPVPVTKKSIYSRDR
Sbjct: 15 GKGKIRFDLSSLSLR*TPS*TLWLHGGSGILQGEEDAIEYAKKHNVPVPVTKKSIYSRDR 194
Query: 202 NLWHITHEGDVLEDPANEPKKDMYMLTVDPEDAPNEPEYVEIGFELGIPVSLNGKRLSPG 261
NLWH++HEGD+LEDPANEPKKDMYM++VDPEDAP++ EY+EIG E G+PVSLNGK LSP
Sbjct: 195 NLWHLSHEGDILEDPANEPKKDMYMMSVDPEDAPDQAEYLEIGIESGLPVSLNGKTLSPA 374
Query: 262 NLVAELNEIGGRHGIGRVDLVEDRIVGMKSRGVYETPGGTILFTAARDLETL-TLDRETL 320
L+AELNEIGG+HGIGR+D+VE+R+VGMKSRGVYETPGGTILF AAR++ T R
Sbjct: 375 TLLAELNEIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFKAAREVGVFDT*SRNNT 554
Query: 321 Q--------LKDSLALKYAELVYAGRWFDPLRESMDSFMEKISQTTTGSVSLKLYKGSAS 372
+ S+ + + RW PL ++ + SV+LKLYKGS +
Sbjct: 555 GERYISP*ICRVSVCW*MGLIHFVSRW-TPL-------CRRLLRPQQVSVTLKLYKGSVT 710
Query: 373 VTGRKSPY 380
V G K +
Sbjct: 711 VAGMKESF 734
Score = 110 bits (275), Expect = 1e-24
Identities = 64/130 (49%), Positives = 79/130 (60%), Gaps = 17/130 (13%)
Frame = +1
Query: 310 LETLTLDRETLQLKDSLALKYAELVYAGRWFDPLRESMDSFMEKISQTTTGSVSLKLYKG 369
LE+LTLDRET+Q+KD+LALKYAELVYAGRW +T V +LY
Sbjct: 520 LESLTLDRETIQVKDTLALKYAELVYAGRWV*----------------STS*VDGRLYAE 651
Query: 370 SASVTGR-----------------KSPYSLYREDISSFESGEIYNHADAVGFIKLYGLPM 412
R KSP+SLYR+DISSFE EIY+ ADA GFI+LYGLP+
Sbjct: 652 DYCDHNRFPLL*NCTKVLLL*LV*KSPFSLYRQDISSFEGSEIYDQADAAGFIRLYGLPL 831
Query: 413 RIRAMMEQGI 422
++RAM+EQG+
Sbjct: 832 KVRAMLEQGL 861
>TC78307 similar to GP|20259085|gb|AAM14258.1 putative argininosuccinate
synthase {Arabidopsis thaliana}, partial (22%)
Length = 660
Score = 214 bits (544), Expect = 7e-56
Identities = 106/130 (81%), Positives = 118/130 (90%), Gaps = 2/130 (1%)
Frame = +2
Query: 1 IKAVLYKD--VEISETKKVAGLKGKLNKVVLAYSGGLDTSVVVPWLRENYGCDVVCFTAD 58
+KAVL D VE+SE KK +GL+GKLN VVLAYSGGLDTS +VPWLRENYGCDVVCFTAD
Sbjct: 269 VKAVLRTDTDVEVSEAKKGSGLRGKLNNVVLAYSGGLDTSAIVPWLRENYGCDVVCFTAD 448
Query: 59 LGQGASELEGLEEKAKASGASQLVVKDLKEEFVRDYVFPCLRAGAIYERKYLLGTAIARP 118
+GQG EL+GLE KAK+SGASQLVVKDLKEEFV+DYVFPCLRAGAIY RKYLLGT++ARP
Sbjct: 449 VGQGIKELDGLEAKAKSSGASQLVVKDLKEEFVKDYVFPCLRAGAIYXRKYLLGTSMARP 628
Query: 119 VIAKAMVDIA 128
VIAKAMVD+A
Sbjct: 629 VIAKAMVDVA 658
>AA661039 similar to GP|20259085|gb| putative argininosuccinate synthase
{Arabidopsis thaliana}, partial (11%)
Length = 655
Score = 112 bits (279), Expect(2) = 1e-41
Identities = 54/76 (71%), Positives = 63/76 (82%), Gaps = 2/76 (2%)
Frame = +2
Query: 1 IKAVLYKD--VEISETKKVAGLKGKLNKVVLAYSGGLDTSVVVPWLRENYGCDVVCFTAD 58
+KAV + D VE+SE +GL+GKLNKVVLAYSGGLDTSV+VPWLR NYGCDVVCFTAD
Sbjct: 236 VKAVSHSDTDVEVSEAXXGSGLRGKLNKVVLAYSGGLDTSVIVPWLRXNYGCDVVCFTAD 415
Query: 59 LGQGASELEGLEEKAK 74
+GQG +L+GLE K K
Sbjct: 416 VGQGIKKLDGLEAKTK 463
Score = 76.3 bits (186), Expect(2) = 1e-41
Identities = 37/59 (62%), Positives = 46/59 (77%)
Frame = +3
Query: 79 SQLVVKDLKEEFVRDYVFPCLRAGAIYERKYLLGTAIARPVIAKAMVDIANEVGADAVS 137
SQLV+KDL EEFV+DY FPCLR+GAI E+K LLGT ARP++ KA ++A + G AVS
Sbjct: 474 SQLVLKDLXEEFVKDYXFPCLRSGAIXEKKLLLGTXWARPLLPKARWNVAKKFGXXAVS 650
>AL387742 similar to PIR|T40457|T40 argininosuccinate synthase - fission
yeast (Schizosaccharomyces pombe), partial (37%)
Length = 488
Score = 159 bits (403), Expect = 1e-39
Identities = 74/160 (46%), Positives = 109/160 (67%), Gaps = 4/160 (2%)
Frame = +3
Query: 65 ELEGLEEKAKASGASQLVVKDLKEEFVRDYVFPCLRAGAIYERKYLLGTAIARPVIAKAM 124
+ E EKA GA ++ ++DL++ +V + +FP ++A AIYE YLLGT++ARPVIA++
Sbjct: 6 DFEAAREKALKIGAKKVYIEDLRQTYVDELIFPAIQANAIYENVYLLGTSLARPVIARSQ 185
Query: 125 VDIANEVGADAVSHGCTGKGNDQVRFELSFFALNPKLNVVAPWR----EWDITGREDAIE 180
++IA + VSHGCTGKGNDQVRFEL+++AL P + V+APWR GR+ ++
Sbjct: 186 IEIATKENCQFVSHGCTGKGNDQVRFELAYYALKPSIKVIAPWRIPKFYESFPGRQSLLD 365
Query: 181 YAKKHDVPVPVTKKSIYSRDRNLWHITHEGDVLEDPANEP 220
YA + ++PV TK +S D NL+HI++E +LEDP P
Sbjct: 366 YAAQRNIPVVQTKSKPWSTDENLFHISYEAGILEDPNVTP 485
>AL387743 weakly similar to PIR|T40457|T404 argininosuccinate synthase -
fission yeast (Schizosaccharomyces pombe), partial (23%)
Length = 523
Score = 68.9 bits (167), Expect = 3e-12
Identities = 39/107 (36%), Positives = 64/107 (59%), Gaps = 2/107 (1%)
Frame = +3
Query: 309 DLETLTLDRETLQLKDS-LALKYAELVYAGRWFDPLRESMDSFMEKISQTTTGSVSLKLY 367
DLE L LDR+ L+D + ++++L+Y G++F P RE + + +E ++ G V LKLY
Sbjct: 12 DLEGLVLDRQVRALRDQFITPQFSQLLYNGQYFSPEREFLSASLEASQKSVNGEVRLKLY 191
Query: 368 KGSASVTGRKS-PYSLYREDISSFESGEIYNHADAVGFIKLYGLPMR 413
KG+ V GRKS LY + SS + ++ D+ GFIK+ + ++
Sbjct: 192 KGNVIVEGRKSDTVKLYDMEESSMDVQGGFSPTDSDGFIKIQSIRLK 332
>TC89526 similar to GP|14596139|gb|AAK68797.1 Unknown protein {Arabidopsis
thaliana}, partial (40%)
Length = 1105
Score = 29.3 bits (64), Expect = 3.0
Identities = 13/42 (30%), Positives = 22/42 (51%)
Frame = +1
Query: 158 NPKLNVVAPWREWDITGREDAIEYAKKHDVPVPVTKKSIYSR 199
NP + P R+ G E ++H++P+PV K S+Y +
Sbjct: 97 NPLTTALQP-RDQTWKGMRTVAELRREHNLPIPVNKDSLYKK 219
>TC85792 homologue to GP|18150857|dbj|BAB83689. mitochondrial phosphate
transporter {Lotus japonicus}, partial (88%)
Length = 1654
Score = 28.9 bits (63), Expect = 3.9
Identities = 16/39 (41%), Positives = 25/39 (64%), Gaps = 2/39 (5%)
Frame = +1
Query: 363 SLKLYKGSASVTGRKSPYSLYREDISSFES--GEIYNHA 399
+L LYKG + GR+ PY++ + +SFE+ +IY HA
Sbjct: 808 ALGLYKGLVPLWGRQIPYTMMK--FASFETIVEQIYKHA 918
>TC78652 similar to PIR|E86270|E86270 hypothetical protein AAF81298.1
[imported] - Arabidopsis thaliana, partial (28%)
Length = 832
Score = 28.5 bits (62), Expect = 5.1
Identities = 19/60 (31%), Positives = 29/60 (47%)
Frame = +3
Query: 202 NLWHITHEGDVLEDPANEPKKDMYMLTVDPEDAPNEPEYVEIGFELGIPVSLNGKRLSPG 261
N+W I+H+ + EDP LT +PE E V +G +L + G+R+ PG
Sbjct: 171 NMWAISHDSSIWEDP----------LTFNPERFLKEDVSV-MGSDLRLAPFGAGRRVCPG 317
>AW584299 similar to GP|20160694|db hypothetical protein~similar to
Arabidopsis thaliana chromosome 3 At3g56840, partial
(22%)
Length = 549
Score = 28.5 bits (62), Expect = 5.1
Identities = 28/111 (25%), Positives = 46/111 (41%)
Frame = +2
Query: 86 LKEEFVRDYVFPCLRAGAIYERKYLLGTAIARPVIAKAMVDIANEVGADAVSHGCTGKGN 145
+ E Y P R + ++G A+AR + K I E A + G + + +
Sbjct: 101 VSRETTTSYSVPRERVDCVVIGAGVVGIAVARALALKGREVIVIE-SAPSFGTGTSSRNS 277
Query: 146 DQVRFELSFFALNPKLNVVAPWREWDITGREDAIEYAKKHDVPVPVTKKSI 196
+ V + + P ++ A + + GRE EY KHD+P T K I
Sbjct: 278 EVVHAGIYY----PHHSLKAIFC---VKGREMLYEYCAKHDIPHEQTGKLI 409
>AL380183
Length = 467
Score = 28.1 bits (61), Expect = 6.7
Identities = 15/40 (37%), Positives = 18/40 (44%)
Frame = -1
Query: 240 YVEIGFELGIPVSLNGKRLSPGNLVAELNEIGGRHGIGRV 279
+ G E+ L R P NL+ L I GR IGRV
Sbjct: 197 FANFGSEIRELKDLQRHRSCPSNLMGSLQRISGRTSIGRV 78
>TC91306 weakly similar to PIR|T06371|T06371 probable
UDP-glucuronosyltransferase (EC 2.4.1.-) - garden pea,
partial (31%)
Length = 724
Score = 27.7 bits (60), Expect = 8.7
Identities = 23/69 (33%), Positives = 34/69 (48%), Gaps = 1/69 (1%)
Frame = +2
Query: 233 DAPNEPEYVEIGFELGIPVSLNGKRLSPGNLVAELNEIGGRHGIGRVDLVEDRIVGMKSR 292
D P Y+ +E+G+ + N KR NLV EL E G G + +I+ MK++
Sbjct: 269 DQPANCRYICNTWEIGMEIDTNVKRDEVENLVNELME--GEKG----KKMWKKIIEMKTK 430
Query: 293 GVYET-PGG 300
+T PGG
Sbjct: 431 AEEDTRPGG 457
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.137 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,645,768
Number of Sequences: 36976
Number of extensions: 124363
Number of successful extensions: 546
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 536
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 544
length of query: 422
length of database: 9,014,727
effective HSP length: 99
effective length of query: 323
effective length of database: 5,354,103
effective search space: 1729375269
effective search space used: 1729375269
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC126782.6