BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126782.12 + phase: 0
(116 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB895143 52 4e-08
BE998238 similar to PIR|T39202|T39 heat shock protein 90 homolog... 33 0.021
CB891165 homologue to PIR|H82068|H820 RNA polymerase-associated ... 31 0.061
TC76341 similar to GP|7528270|gb|AAF63202.1| poly(A)-binding pro... 31 0.080
TC93879 similar to GP|3877368|emb|CAA92514.1 cDNA EST EMBL:T0125... 30 0.10
TC92792 similar to GP|15294158|gb|AAK95256.1 unknown protein {Ar... 30 0.18
TC91613 similar to GP|19347756|gb|AAL86302.1 unknown protein {Ar... 30 0.18
AW736138 similar to PIR|E84800|E848 hypothetical protein At2g380... 28 0.40
TC78204 similar to GP|20197010|gb|AAM14872.1 Expressed protein {... 28 0.40
TC89538 weakly similar to GP|21689895|gb|AAM67508.1 unknown prot... 28 0.52
TC83465 weakly similar to GP|18252903|gb|AAL62378.1 putative pro... 28 0.68
TC81765 similar to GP|12323731|gb|AAG51827.1 hypothetical protei... 27 0.88
TC78370 similar to GP|5001828|gb|AAD37165.1| 3-phosphoinositide-... 27 0.88
TC85290 similar to GP|7767653|gb|AAF69150.1| F27F5.2 {Arabidopsi... 27 1.2
TC79376 homologue to GP|13877645|gb|AAK43900.1 Unknown protein {... 27 1.5
TC83887 weakly similar to GP|13310891|gb|AAK13153.2 hypothetical... 27 1.5
TC83524 weakly similar to GP|8164030|gb|AAF73960.1| SGS3 {Arabid... 26 2.0
TC91529 similar to GP|21553452|gb|AAM62545.1 unknown {Arabidopsi... 26 2.0
AL385482 similar to GP|5106924|gb|A putative cell wall protein {... 26 2.6
TC90410 similar to PIR|T04638|T04638 hypothetical protein F10N7.... 26 2.6
>CB895143
Length = 770
Score = 51.6 bits (122), Expect = 4e-08
Identities = 30/61 (49%), Positives = 40/61 (65%), Gaps = 6/61 (9%)
Frame = +2
Query: 1 MGGNVGSGSSRSKRSHK------DSVGSSARPMGREAAKKKGIKKSKDVAGLEEVEKEWV 54
+GGN GSGSS SKR+HK +SVGSS+ PMGR+AAKK+ +K G+ + E +
Sbjct: 575 VGGNTGSGSSGSKRAHKSDASDSNSVGSSSCPMGRDAAKKR*KEKQGRNLGISQ*RVE*I 754
Query: 55 Q 55
Q
Sbjct: 755 Q 757
Score = 32.3 bits (72), Expect = 0.027
Identities = 17/28 (60%), Positives = 20/28 (70%)
Frame = +3
Query: 34 KKGIKKSKDVAGLEEVEKEWVQFKEIKE 61
KKG KKSK A LE V +EW +FK+ KE
Sbjct: 690 KKGEKKSKG-ATLESVNEEWNEFKQCKE 770
>BE998238 similar to PIR|T39202|T39 heat shock protein 90 homolog - fission
yeast (Schizosaccharomyces pombe), partial (30%)
Length = 654
Score = 32.7 bits (73), Expect = 0.021
Identities = 19/64 (29%), Positives = 37/64 (57%)
Frame = +3
Query: 47 EEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNRLKKMKMYLKLSSEEHLDDRKKEHLVKH 106
+EVEKE +E+KE+E + +V+ E+++ +K K +K + E+ + K +K
Sbjct: 102 KEVEKEVDDDEEVKEEEDGEKPKIEEVEDEESKKEKKKKKVKQTEVENQELNK----MKP 269
Query: 107 IWTK 110
+WT+
Sbjct: 270 LWTR 281
>CB891165 homologue to PIR|H82068|H820 RNA polymerase-associated protein HepA
VC2506 [imported] - Vibrio cholerae, partial (1%)
Length = 692
Score = 31.2 bits (69), Expect = 0.061
Identities = 22/68 (32%), Positives = 35/68 (51%)
Frame = -2
Query: 33 KKKGIKKSKDVAGLEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNRLKKMKMYLKLSSE 92
KK+ K+ + E+ K V+ E E++I L E +KN ++ K+ LK S E
Sbjct: 442 KKQKKSMMKEKSQFEDEAKRLVEEGEKVEEKIRILVE------QKNSIELEKIKLKESME 281
Query: 93 EHLDDRKK 100
H D++KK
Sbjct: 280 RHEDEKKK 257
>TC76341 similar to GP|7528270|gb|AAF63202.1| poly(A)-binding protein {Cucumis
sativus}, partial (90%)
Length = 2501
Score = 30.8 bits (68), Expect = 0.080
Identities = 19/67 (28%), Positives = 36/67 (53%), Gaps = 4/67 (5%)
Frame = +3
Query: 50 EKEW----VQFKEIKEQEIEHLKEYTKVQQEKNRLKKMKMYLKLSSEEHLDDRKKEHLVK 105
+KEW Q K +EQE++ E T + ++ + + +YLK +LDD + +K
Sbjct: 1032 DKEWYVGKAQKKSEREQELKGRFEQTVKESVVDKFQGLNLYLK-----NLDDSITDEKLK 1196
Query: 106 HIWTKFG 112
++++FG
Sbjct: 1197 EMFSEFG 1217
>TC93879 similar to GP|3877368|emb|CAA92514.1 cDNA EST EMBL:T01259 comes
from this gene~cDNA EST yk67d3.3 comes from this
gene~cDNA EST yk67d3.5, partial (68%)
Length = 426
Score = 30.4 bits (67), Expect = 0.10
Identities = 13/42 (30%), Positives = 27/42 (63%)
Frame = +1
Query: 28 GREAAKKKGIKKSKDVAGLEEVEKEWVQFKEIKEQEIEHLKE 69
GR+ K K +KK+K +++++ +FK+ +++E + LKE
Sbjct: 25 GRQGGKLKPLKKTKKDNNKDDLDENDAEFKKKQQEEAKKLKE 150
>TC92792 similar to GP|15294158|gb|AAK95256.1 unknown protein {Arabidopsis
thaliana}, partial (82%)
Length = 599
Score = 29.6 bits (65), Expect = 0.18
Identities = 14/41 (34%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Frame = -3
Query: 60 KEQEIEHLKEYTKVQQEKNRLKKMK-MYLKLSSEEHLDDRK 99
KEQ+++HL+E+ QEKN K K ++ + HL ++
Sbjct: 282 KEQQLQHLQEHDLQDQEKNHHKHHKQLFHEFHQSNHLHSKQ 160
>TC91613 similar to GP|19347756|gb|AAL86302.1 unknown protein {Arabidopsis
thaliana}, partial (57%)
Length = 657
Score = 29.6 bits (65), Expect = 0.18
Identities = 16/52 (30%), Positives = 29/52 (55%)
Frame = +2
Query: 41 KDVAGLEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNRLKKMKMYLKLSSE 92
+ + GL + W+ +E K Q + +K K+Q +K R KKMKM + + ++
Sbjct: 200 QQILGLNRFFEPWL*SEEEKGQNQKRVK--AKIQNQKFRKKKMKMMIMIRTK 349
>AW736138 similar to PIR|E84800|E848 hypothetical protein At2g38070
[imported] - Arabidopsis thaliana, partial (7%)
Length = 604
Score = 28.5 bits (62), Expect = 0.40
Identities = 12/28 (42%), Positives = 21/28 (74%)
Frame = +2
Query: 61 EQEIEHLKEYTKVQQEKNRLKKMKMYLK 88
+QEIE ++E TKV++++ RL++ Y K
Sbjct: 359 QQEIEEMEEETKVEEKQCRLRRRCCYRK 442
>TC78204 similar to GP|20197010|gb|AAM14872.1 Expressed protein {Arabidopsis
thaliana}, partial (45%)
Length = 1313
Score = 28.5 bits (62), Expect = 0.40
Identities = 22/91 (24%), Positives = 41/91 (44%)
Frame = +3
Query: 18 DSVGSSARPMGREAAKKKGIKKSKDVAGLEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEK 77
+ V S + REA + IKKSK+ ++ E +Q ++ +E++I
Sbjct: 726 NEVSSEKKKRKREALEGDVIKKSKNDTSVDAENGEVIQGEKARERKI------------- 866
Query: 78 NRLKKMKMYLKLSSEEHLDDRKKEHLVKHIW 108
K K ++K + + H D K + L K ++
Sbjct: 867 ----KWKKFIKAALKSHPDGLKMKKLRKVVF 947
>TC89538 weakly similar to GP|21689895|gb|AAM67508.1 unknown protein
{Arabidopsis thaliana}, partial (37%)
Length = 990
Score = 28.1 bits (61), Expect = 0.52
Identities = 19/65 (29%), Positives = 34/65 (52%)
Frame = -1
Query: 11 RSKRSHKDSVGSSARPMGREAAKKKGIKKSKDVAGLEEVEKEWVQFKEIKEQEIEHLKEY 70
R +R S G R +E +++ KK ++ EEVEKEW + ++ + QE ++
Sbjct: 366 RWRRI*GSSSG*GKRWKKKEEVEEEWRKKVEEWRKKEEVEKEWRKEEDEE*QERRWMRNV 187
Query: 71 TKVQQ 75
KV++
Sbjct: 186 KKVEE 172
Score = 23.9 bits (50), Expect = 9.8
Identities = 14/42 (33%), Positives = 25/42 (59%), Gaps = 4/42 (9%)
Frame = -1
Query: 47 EEVEKEW----VQFKEIKEQEIEHLKEYTKVQQEKNRLKKMK 84
EEVE+EW ++++ +E E E KE + QE+ ++ +K
Sbjct: 309 EEVEEEWRKKVEEWRKKEEVEKEWRKEEDEE*QERRWMRNVK 184
>TC83465 weakly similar to GP|18252903|gb|AAL62378.1 putative protein
{Arabidopsis thaliana}, partial (32%)
Length = 1009
Score = 27.7 bits (60), Expect = 0.68
Identities = 20/60 (33%), Positives = 30/60 (49%)
Frame = +2
Query: 29 REAAKKKGIKKSKDVAGLEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNRLKKMKMYLK 88
REA KK KK + A LEE K + K + +Q + L+ ++ E RL+ + LK
Sbjct: 710 REAVKKYREKKKAETAYLEEEVK---KLKLVNQQLLRKLQGQALLEAELLRLRNILXQLK 880
>TC81765 similar to GP|12323731|gb|AAG51827.1 hypothetical protein;
55509-55015 {Arabidopsis thaliana}, partial (25%)
Length = 710
Score = 27.3 bits (59), Expect = 0.88
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +1
Query: 76 EKNRLKKMKMYLKLSSEEHLDDRKKEHL 103
E ++ YLKLSSEE + +R KE L
Sbjct: 31 ESRSSSSVREYLKLSSEEEMAERSKELL 114
>TC78370 similar to GP|5001828|gb|AAD37165.1| 3-phosphoinositide-dependent
protein kinase-1 {Arabidopsis thaliana}, partial (91%)
Length = 2137
Score = 27.3 bits (59), Expect = 0.88
Identities = 20/78 (25%), Positives = 35/78 (44%), Gaps = 3/78 (3%)
Frame = +1
Query: 6 GSGSSRSKRSHK-DSVGSSARPMGREAAKKKGIKKSKDVAGLEEVEKEWVQFKEIKEQ-- 62
G+G S +H D +SAR + +G +A ++ + +W QF E E
Sbjct: 1339 GAGESSWSPAHTGDGSAASARQPDGATSSSEGAGSITRLASIDSFDSKWQQFLEPGESVL 1518
Query: 63 EIEHLKEYTKVQQEKNRL 80
I +K+ K+ +K +L
Sbjct: 1519 MISMVKKLQKITSKKVQL 1572
>TC85290 similar to GP|7767653|gb|AAF69150.1| F27F5.2 {Arabidopsis
thaliana}, partial (32%)
Length = 1558
Score = 26.9 bits (58), Expect = 1.2
Identities = 14/45 (31%), Positives = 25/45 (55%)
Frame = +3
Query: 57 KEIKEQEIEHLKEYTKVQQEKNRLKKMKMYLKLSSEEHLDDRKKE 101
KEI E+ I +LKE K ++ K +K K + +E +++K+
Sbjct: 687 KEIFEEYITYLKEKAKEKERKREEEKAKKEKEREEKEKRKEKEKK 821
>TC79376 homologue to GP|13877645|gb|AAK43900.1 Unknown protein {Arabidopsis
thaliana}, partial (29%)
Length = 952
Score = 26.6 bits (57), Expect = 1.5
Identities = 21/74 (28%), Positives = 32/74 (42%)
Frame = +3
Query: 30 EAAKKKGIKKSKDVAGLEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNRLKKMKMYLKL 89
EA KK IKK + + K W+Q EIK++++ E V K ++M+ +
Sbjct: 336 EADLKKEIKK---LQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIEREMERFKIC 506
Query: 90 SSEEHLDDRKKEHL 103
E KE L
Sbjct: 507 EKETKTKAFSKEGL 548
>TC83887 weakly similar to GP|13310891|gb|AAK13153.2 hypothetical protein
{Oryza sativa (japonica cultivar-group)}, partial (8%)
Length = 778
Score = 26.6 bits (57), Expect = 1.5
Identities = 23/99 (23%), Positives = 41/99 (41%), Gaps = 4/99 (4%)
Frame = +2
Query: 6 GSGSSRSKRSHKDSVGSSARPMGREAAKKKGIK--KSKDVAGLEEVEKEW--VQFKEIKE 61
G+G + +R K RE K++G+K K + + G+E+ W ++ +K+
Sbjct: 275 GNGKRKGQRRRKIGRKEKEGNRSREGKKREGVKDGKRRHIRGIEQTVTVWT*MRSTSLKK 454
Query: 62 QEIEHLKEYTKVQQEKNRLKKMKMYLKLSSEEHLDDRKK 100
+ +K T L K+ + H DRKK
Sbjct: 455 TKGSSIKVRTMF------LSKLIKREPRNLHGHSSDRKK 553
>TC83524 weakly similar to GP|8164030|gb|AAF73960.1| SGS3 {Arabidopsis
thaliana}, partial (22%)
Length = 1231
Score = 26.2 bits (56), Expect = 2.0
Identities = 12/58 (20%), Positives = 33/58 (56%)
Frame = +2
Query: 44 AGLEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNRLKKMKMYLKLSSEEHLDDRKKE 101
+G +++ E ++E+ E +I+H+ + +KV ++ + + ++ ++EH K+E
Sbjct: 506 SGKSKLKFEIRSYQEMVESKIKHINDDSKVSEKLRKTTEENHVVQERTKEHHLQNKEE 679
>TC91529 similar to GP|21553452|gb|AAM62545.1 unknown {Arabidopsis
thaliana}, partial (63%)
Length = 833
Score = 26.2 bits (56), Expect = 2.0
Identities = 15/51 (29%), Positives = 24/51 (46%)
Frame = +1
Query: 29 REAAKKKGIKKSKDVAGLEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNR 79
+EA +KK +KK K E+EW E L E ++++EK +
Sbjct: 547 QEAKRKKNVKKVKRKMAAVAREREWA----------ERLVELQRLEEEKKK 669
>AL385482 similar to GP|5106924|gb|A putative cell wall protein {Medicago
truncatula}, partial (42%)
Length = 402
Score = 25.8 bits (55), Expect = 2.6
Identities = 14/59 (23%), Positives = 30/59 (50%)
Frame = +3
Query: 47 EEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNRLKKMKMYLKLSSEEHLDDRKKEHLVK 105
+++ K ++ K + Q+ EH + QQE++ ++ E+H RK+ HL++
Sbjct: 24 KKLPKMLLKVK*LHHQQEEHHHQEEHHQQEEHHHQEEHQQDHHQKEQHQHQRKRPHLLQ 200
>TC90410 similar to PIR|T04638|T04638 hypothetical protein F10N7.120 -
Arabidopsis thaliana, partial (23%)
Length = 1983
Score = 25.8 bits (55), Expect = 2.6
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = +1
Query: 33 KKKGIKKSKDVAGLEEVEKEWVQFKEIKEQE 63
K+K KSK+ A E+++K+ KE+ E++
Sbjct: 46 KEKKSNKSKNKAAKEKIDKQKEDVKEVNEEK 138
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.309 0.127 0.349
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,080,044
Number of Sequences: 36976
Number of extensions: 31558
Number of successful extensions: 226
Number of sequences better than 10.0: 75
Number of HSP's better than 10.0 without gapping: 223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 225
length of query: 116
length of database: 9,014,727
effective HSP length: 92
effective length of query: 24
effective length of database: 5,612,935
effective search space: 134710440
effective search space used: 134710440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 50 (23.9 bits)
Medicago: description of AC126782.12