BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126019.1 + phase: 0
(253 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC89025 similar to GP|2462911|emb|CAB06081.1 UDP-glucose:sterol ... 449 e-127
BQ148792 similar to PIR|D96499|D96 probable UDP-glucose sterol g... 242 1e-64
TC82528 weakly similar to PIR|C86356|C86356 hypothetical protein... 35 0.028
TC82794 weakly similar to GP|3928543|dbj|BAA34687.1 UDP-glucose ... 29 1.5
AW692390 similar to SP|Q9SGE9|SYFB Probable phenylalanyl-tRNA sy... 28 4.4
TC78934 similar to GP|14189890|dbj|BAB55874. response regulator ... 28 4.4
TC89542 homologue to PIR|F96805|F96805 unknown protein T5M16.20 ... 28 4.4
TC78695 similar to GP|4115563|dbj|BAA36423.1 UDP-glucose:anthocy... 27 5.8
TC89356 similar to GP|19911207|dbj|BAB86930. glucosyltransferase... 27 5.8
BF645180 weakly similar to GP|16226290|gb At1g18480/F15H18_1 {Ar... 27 5.8
TC91201 similar to PIR|T06371|T06371 probable UDP-glucuronosyltr... 27 5.8
CA920602 similar to GP|19911211|db glucosyltransferase-14 {Vigna... 27 7.5
TC81538 similar to GP|9758506|dbj|BAB08914.1 senescence-associat... 27 9.9
>TC89025 similar to GP|2462911|emb|CAB06081.1 UDP-glucose:sterol
glucosyltransferase {Avena sativa}, partial (47%)
Length = 1157
Score = 449 bits (1155), Expect = e-127
Identities = 211/226 (93%), Positives = 217/226 (95%)
Frame = +1
Query: 28 DWGPKIDVVGFCFLDLASNYEPPESLVKWLEDGDKPIYVGFGSLPVQDPKTMTEIIVEAL 87
DWGPKIDVVGFCFLDLASNYEPPESLVKWLEDGDKPIY+GFGSLPVQDPK MT+IIVEAL
Sbjct: 274 DWGPKIDVVGFCFLDLASNYEPPESLVKWLEDGDKPIYIGFGSLPVQDPKKMTQIIVEAL 453
Query: 88 ETTGQRGIISKGWGGLGDLTEPKDSIYLLDNVPHDWLFLHCKAVVHHGGAGTTAAGLKAA 147
ETTGQRGII+KGWGGLGDLTEPKDSIYLLDNVPHDWLFL CKAVVHHGGAGTTAAGLKAA
Sbjct: 454 ETTGQRGIINKGWGGLGDLTEPKDSIYLLDNVPHDWLFLQCKAVVHHGGAGTTAAGLKAA 633
Query: 148 CPTTIVPFFGDQPFWGERVHDRGVGPPPIPIDVFSLPKLIAAINFMLDPKVKEHAIELAK 207
CPTTIVPFFGDQPFWGERVHDRGVGPPPIP+D FSLPKLI AINFMLDPKVKEHAIELAK
Sbjct: 634 CPTTIVPFFGDQPFWGERVHDRGVGPPPIPVDEFSLPKLIDAINFMLDPKVKEHAIELAK 813
Query: 208 AMENEDGVTGAVKVFFKQLPKNKPEPNTEPSSSSCFSYIARCFGYS 253
AMENEDGVTGAVK FFKQLP+ KPE NTEPS SSCFS IARCFG+S
Sbjct: 814 AMENEDGVTGAVKAFFKQLPQKKPETNTEPSPSSCFSNIARCFGHS 951
>BQ148792 similar to PIR|D96499|D96 probable UDP-glucose sterol
glucosyltransferase [imported] - Arabidopsis thaliana,
partial (31%)
Length = 701
Score = 242 bits (617), Expect = 1e-64
Identities = 108/194 (55%), Positives = 140/194 (71%)
Frame = +3
Query: 40 FLDLASNYEPPESLVKWLEDGDKPIYVGFGSLPVQDPKTMTEIIVEALETTGQRGIISKG 99
FL SNY+P E + W++ G P+Y GFGS+P++DPK T++I++AL+ T QRGII +G
Sbjct: 3 FLRHESNYQPREDFLHWIKKGPPPLYFGFGSMPLEDPKITTDVILKALKETEQRGIIDRG 182
Query: 100 WGGLGDLTEPKDSIYLLDNVPHDWLFLHCKAVVHHGGAGTTAAGLKAACPTTIVPFFGDQ 159
WG LG+LTE D+++LL+ PHDWLF C AVVHHGGAGTTA GLK+ CPTTIVPFFGDQ
Sbjct: 183 WGNLGNLTEVSDNVFLLEECPHDWLFPQCSAVVHHGGAGTTATGLKSGCPTTIVPFFGDQ 362
Query: 160 PFWGERVHDRGVGPPPIPIDVFSLPKLIAAINFMLDPKVKEHAIELAKAMENEDGVTGAV 219
FWG+R+H + +GP PIPI ++ L AI FML P+VK +E+AK +E+EDGV V
Sbjct: 363 FFWGDRIHQKELGPAPIPISELNVENLSNAIKFMLQPEVKSRTMEVAKLIESEDGVAAXV 542
Query: 220 KVFFKQLPKNKPEP 233
F + LP P P
Sbjct: 543 DAFHRHLPDELPLP 584
>TC82528 weakly similar to PIR|C86356|C86356 hypothetical protein AAF87257.1
[imported] - Arabidopsis thaliana, partial (35%)
Length = 1376
Score = 35.0 bits (79), Expect = 0.028
Identities = 34/136 (25%), Positives = 54/136 (39%), Gaps = 18/136 (13%)
Frame = +1
Query: 43 LASN-YEPPESLVKWLE--DGDKPIYVGFGSLPVQDPKTMTEI-------------IVEA 86
L+SN ++ ++WLE + + +YV FGS+ V P + E I+
Sbjct: 643 LSSNLWKEDTKCLEWLESKEPESVVYVNFGSITVMTPNQLLEFAWGLADSKKPFLWIIRP 822
Query: 87 LETTGQRGIISKGWGGLGDLTEPKDSIYLLDNVPHDWLFLHCK--AVVHHGGAGTTAAGL 144
G I+S + E D + P + + +H + H G +T +
Sbjct: 823 DLVIGGSFILSSEFEN-----EISDRGLITSWCPQEQVLIHPSIGGFLTHCGWNSTTESI 987
Query: 145 KAACPTTIVPFFGDQP 160
A P PFFGDQP
Sbjct: 988 CAGVPMLCWPFFGDQP 1035
>TC82794 weakly similar to GP|3928543|dbj|BAA34687.1 UDP-glucose
glucosyltransferase {Arabidopsis thaliana}, partial
(59%)
Length = 1309
Score = 29.3 bits (64), Expect = 1.5
Identities = 29/124 (23%), Positives = 47/124 (37%), Gaps = 17/124 (13%)
Frame = +1
Query: 54 VKWLEDGDKP--IYVGFGSLPVQDPKTMTEI-------------IVEALETTGQRGIISK 98
++WLE ++ +YV FGS+ V P + E I+ G I+S
Sbjct: 835 LEWLESKEQGSVVYVNFGSITVMTPDQLLEFAWGLANSKKPFLWIIRPDLVIGGSVILSS 1014
Query: 99 GWGGLGDLTEPKDSIYLLDNVPHDWLFLHCKA--VVHHGGAGTTAAGLKAACPTTIVPFF 156
+ + E D + P + + H + H G +T + A P PFF
Sbjct: 1015DF-----VNETSDRGVIASWCPQEKVLNHPSVGGFLTHCGWNSTMESICAGVPMLCWPFF 1179
Query: 157 GDQP 160
+QP
Sbjct: 1180AEQP 1191
>AW692390 similar to SP|Q9SGE9|SYFB Probable phenylalanyl-tRNA synthetase
beta chain (EC 6.1.1.20), partial (33%)
Length = 656
Score = 27.7 bits (60), Expect = 4.4
Identities = 17/45 (37%), Positives = 22/45 (48%), Gaps = 1/45 (2%)
Frame = -3
Query: 2 CNLLYIFKKLTQVILLSIKYIIFLFADWGPKIDVVGF-CFLDLAS 45
CNL + KL + L +K + WG DV GF C L +AS
Sbjct: 492 CNLSWRSMKLLYLTLSKVKPLSTAQTTWGRIRDVSGFTCILSIAS 358
>TC78934 similar to GP|14189890|dbj|BAB55874. response regulator 9 {Zea
mays}, partial (25%)
Length = 1014
Score = 27.7 bits (60), Expect = 4.4
Identities = 11/23 (47%), Positives = 16/23 (68%)
Frame = -1
Query: 160 PFWGERVHDRGVGPPPIPIDVFS 182
PF+ ERV+ G G P +P+ +FS
Sbjct: 600 PFFSERVYTSGDGLPRLPLVIFS 532
>TC89542 homologue to PIR|F96805|F96805 unknown protein T5M16.20 [imported]
- Arabidopsis thaliana, partial (58%)
Length = 854
Score = 27.7 bits (60), Expect = 4.4
Identities = 10/29 (34%), Positives = 16/29 (54%)
Frame = -3
Query: 34 DVVGFCFLDLASNYEPPESLVKWLEDGDK 62
D+ G F L + Y P +KW++D D+
Sbjct: 441 DMSGLSFSTLITTYAPMAEQIKWIQDTDR 355
>TC78695 similar to GP|4115563|dbj|BAA36423.1 UDP-glucose:anthocyanin
5-O-glucosyltransferase {Verbena x hybrida}, partial
(49%)
Length = 1741
Score = 27.3 bits (59), Expect = 5.8
Identities = 13/41 (31%), Positives = 23/41 (55%), Gaps = 2/41 (4%)
Frame = +1
Query: 51 ESLVKWLEDGDKP--IYVGFGSLPVQDPKTMTEIIVEALET 89
+ ++WL+ D+ +YV FG+L V + M EI L++
Sbjct: 868 DDYIQWLDSKDEKSVVYVSFGTLAVLSKRQMEEIARALLDS 990
>TC89356 similar to GP|19911207|dbj|BAB86930. glucosyltransferase-12 {Vigna
angularis}, partial (58%)
Length = 1642
Score = 27.3 bits (59), Expect = 5.8
Identities = 33/140 (23%), Positives = 55/140 (38%), Gaps = 11/140 (7%)
Frame = +1
Query: 40 FLDLASNYEPPESLVKWLEDGD--KPIYVGFGSLPVQDPKTMTEIIVEALETTGQ----- 92
F ++ ++ + + WL+ IYV FGS + + EI L + Q
Sbjct: 829 FFSKSNFFKVDRTCMAWLDSQPLKSVIYVSFGSTTPMKREEIIEIWHGLLNSKKQFLWVI 1008
Query: 93 RGIISKGWGGLGDLTEP--KDSIYLLDNVPHDWLFLH--CKAVVHHGGAGTTAAGLKAAC 148
R + + G L +L E K+ ++ VP + + H A + H G +T +
Sbjct: 1009RPNMVQEKGLLSELEEGTRKEKGLIVGWVPQEEVLSHKAIGAFLTHNGWNSTLESVVCGV 1188
Query: 149 PTTIVPFFGDQPFWGERVHD 168
P P+F DQ V D
Sbjct: 1189PMICWPYFADQQINSRFVSD 1248
>BF645180 weakly similar to GP|16226290|gb At1g18480/F15H18_1 {Arabidopsis
thaliana}, partial (32%)
Length = 650
Score = 27.3 bits (59), Expect = 5.8
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +2
Query: 173 PPPIPIDVFSLPKLIAAINFMLDPKVKEHAIELAKAMENEDGVTG 217
PPPIP + S +LIA + D K + A+ +A +++ TG
Sbjct: 155 PPPIPTRLPSPSRLIAIGDLHGDLKKSKEALSIAGLIDSSGNYTG 289
>TC91201 similar to PIR|T06371|T06371 probable UDP-glucuronosyltransferase
(EC 2.4.1.-) - garden pea, partial (63%)
Length = 822
Score = 27.3 bits (59), Expect = 5.8
Identities = 30/129 (23%), Positives = 49/129 (37%), Gaps = 17/129 (13%)
Frame = +3
Query: 48 EPPESLVKWL--EDGDKPIYVGFGSLPVQDPKTMTEI-------------IVEALETTGQ 92
E PE L +WL ++ + +YV FGS+ V + M E ++ G+
Sbjct: 72 EEPECL-EWLNSKEPNSVVYVNFGSITVMTSEQMIEFAWGLSNSKMPFLWVIRPDLVAGE 248
Query: 93 RGIISKGWGGLGDLTEPKDSIYLLDNVPHDWLFLHCK--AVVHHGGAGTTAAGLKAACPT 150
++ L L E K+ L P + + H + H G +T + P
Sbjct: 249 NAVLP-----LEFLEETKNRGLLSSWCPQEEVLGHSSIGGFLTHNGWNSTLESVCGGVPM 413
Query: 151 TIVPFFGDQ 159
PFF +Q
Sbjct: 414 ICWPFFAEQ 440
>CA920602 similar to GP|19911211|db glucosyltransferase-14 {Vigna angularis},
partial (35%)
Length = 817
Score = 26.9 bits (58), Expect = 7.5
Identities = 21/78 (26%), Positives = 29/78 (36%)
Frame = -3
Query: 88 ETTGQRGIISKGWGGLGDLTEPKDSIYLLDNVPHDWLFLHCKAVVHHGGAGTTAAGLKAA 147
E T +G + KGW + +L + HC G +T + A
Sbjct: 689 ERTKGKGFLIKGWA---------PQVLILSHFSVGGFLTHC-------GWNSTLEAICAG 558
Query: 148 CPTTIVPFFGDQPFWGER 165
P P FGDQ F+ ER
Sbjct: 557 VPMITWPLFGDQ-FFNER 507
>TC81538 similar to GP|9758506|dbj|BAB08914.1 senescence-associated protein
5-like protein {Arabidopsis thaliana}, partial (92%)
Length = 1073
Score = 26.6 bits (57), Expect = 9.9
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = +3
Query: 226 LPKNKPEPNTEPSSSSCFSYIARCFGY 252
L +N PN PS +C Y+A FGY
Sbjct: 228 LLENLMSPNILPSCYACAYYLASLFGY 308
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.142 0.453
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,736,582
Number of Sequences: 36976
Number of extensions: 142938
Number of successful extensions: 730
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 725
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 728
length of query: 253
length of database: 9,014,727
effective HSP length: 94
effective length of query: 159
effective length of database: 5,538,983
effective search space: 880698297
effective search space used: 880698297
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC126019.1