BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126012.8 + phase: 0
(440 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79804 weakly similar to GP|6175165|gb|AAF04891.1| Mutator-like... 130 1e-30
CB891859 similar to GP|6175165|gb| Mutator-like transposase {Ara... 69 4e-12
BG451959 homologue to GP|6175165|gb| Mutator-like transposase {A... 67 1e-11
AL375901 similar to GP|9759134|dbj mutator-like transposase-like... 61 7e-10
AL375902 similar to GP|9759134|dbj mutator-like transposase-like... 50 1e-06
BI309424 SP|P03749|VHS Host specificity protein J. {Bacteriophag... 35 0.075
CB065281 similar to SP|Q51422|SYD_ Aspartyl-tRNA synthetase (EC ... 31 0.83
TC79947 similar to GP|15450948|gb|AAK96745.1 Unknown protein {Ar... 31 1.1
TC87026 weakly similar to GP|21592576|gb|AAM64525.1 SOUL-like pr... 30 1.4
TC81234 similar to GP|6752888|gb|AAF27920.1| unknown {Malus x do... 30 1.8
TC86301 similar to GP|8809622|dbj|BAA97173.1 Myb-related transcr... 29 3.2
TC81134 weakly similar to GP|14334664|gb|AAK59510.1 unknown prot... 29 3.2
TC88387 similar to GP|13357253|gb|AAK20050.1 putative zinc finge... 29 4.1
BF632724 similar to GP|20804478|db hypothetical protein~similar ... 28 5.4
TC78787 similar to GP|13569996|gb|AAK31280.1 putative Myb-relate... 28 9.2
TC86179 similar to GP|6752888|gb|AAF27920.1| unknown {Malus x do... 28 9.2
TC86178 similar to GP|6752888|gb|AAF27920.1| unknown {Malus x do... 28 9.2
TC87639 GP|9663153|emb|CAC01132.1 transport-secretion protein 2.... 28 9.2
>TC79804 weakly similar to GP|6175165|gb|AAF04891.1| Mutator-like
transposase {Arabidopsis thaliana}, partial (57%)
Length = 2129
Score = 130 bits (326), Expect = 1e-30
Identities = 88/327 (26%), Positives = 152/327 (45%), Gaps = 7/327 (2%)
Frame = +1
Query: 26 AQFHRLFWAFYPCINGF-KYCKPVVHVDGTWLYDKYKGTLLLAVAQDGNNKTIPIAFALV 84
++F RLF +FY I+GF C P+V + G L KY T L A + D + P+AFA+V
Sbjct: 688 SRFQRLFISFYASIHGFVNGCLPIVALGGIQLKSKYLSTFLSATSFDADGGLFPLAFAVV 867
Query: 85 EGETKEGWSFFLKNLRRHVTKGIS----VCMVSDRHESIKSAFND--PRNGWQATGSAHV 138
+ E E W++FL L + + +SD + I A PR S+H
Sbjct: 868 DVENDESWTWFLSELHNALEVNTECMPQIIFLSDGQKGIVDAIRRKFPR-------SSHA 1026
Query: 139 YCIRHIKQNFMRTIKDGDLKDVVNNMGYALNVPLFNYYRGVIQEANQRALDWVDNIPKEK 198
+C+RH+ +N + K+ L ++ + YA + F I+E + A W+ + +
Sbjct: 1027FCMRHLSENIGKEFKNSRLIHLLWSAAYATTINAFREKMAEIEEVSPNASMWLQHFHPSQ 1206
Query: 199 WTQAYDEGRRWGHMTSNIVESWNSVFKGTRNLPVTPIVQSTYYRLACLFADRAQKAFARV 258
W Y EG R+GH++SNI E +N + LP+ +++ +L F DR K+ +
Sbjct: 1207WALVYFEGTRYGHLSSNI-EEFNKWILEAQELPIIQVIERIQSKLKTEFDDRRLKSSSWC 1383
Query: 259 GSGDLFSEYCQNAIQDDIVKSNTHHVEQFDRERYTFSVRETVNYREGRPMGTFKVDLRAG 318
+ + + + + I +++T+ V + D + + + V++ +
Sbjct: 1384---SVLTPSSERRMVEAINRASTYQVLKSDEVEFEVISADRSDI----------VNIGSH 1524
Query: 319 WCDCGKFQALHLPCSHVIAACSSFRHD 345
C C +Q +PCSH +AA S R D
Sbjct: 1525SCSCRDWQLYGIPCSHAVAALISSRKD 1605
>CB891859 similar to GP|6175165|gb| Mutator-like transposase {Arabidopsis
thaliana}, partial (27%)
Length = 625
Score = 68.9 bits (167), Expect = 4e-12
Identities = 44/186 (23%), Positives = 86/186 (45%)
Frame = +1
Query: 137 HVYCIRHIKQNFMRTIKDGDLKDVVNNMGYALNVPLFNYYRGVIQEANQRALDWVDNIPK 196
H +C+RH+ +F + + L +++ L + F I+E +Q A W+ +P
Sbjct: 103 HGFCMRHLSDSFRKEFNNTMLVNLLWEAANCLTIIEFEGKVMEIEEISQDAAYWIRRVPP 282
Query: 197 EKWTQAYDEGRRWGHMTSNIVESWNSVFKGTRNLPVTPIVQSTYYRLACLFADRAQKAFA 256
W AY EG R+GH+T+NIVE+ NS LP+ +++ +L F +R + +
Sbjct: 283 RLWATAYFEGHRFGHLTANIVEALNSWILEASGLPIIQMMECIRRQLMTWFNERRETSMQ 462
Query: 257 RVGSGDLFSEYCQNAIQDDIVKSNTHHVEQFDRERYTFSVRETVNYREGRPMGTFKVDLR 316
+ + ++ + + ++ T+ V + + + E +++ GT VD+R
Sbjct: 463 WT---SILVPSAERSVAEALERARTYQVLRANEAEF-----EVISHE-----GTNIVDIR 603
Query: 317 AGWCDC 322
C C
Sbjct: 604 NRCCLC 621
>BG451959 homologue to GP|6175165|gb| Mutator-like transposase {Arabidopsis
thaliana}, partial (28%)
Length = 658
Score = 67.4 bits (163), Expect = 1e-11
Identities = 42/130 (32%), Positives = 66/130 (50%), Gaps = 7/130 (5%)
Frame = +2
Query: 28 FHRLFWAFYPCINGF-KYCKPVVHVDGTWLYDKYKGTLLLAVAQDGNNKTIPIAFALVEG 86
F RLF +F I G C+P++ +D +L KY GTLLLA DG+ P+AF +V+
Sbjct: 287 FQRLFISFQASIYGLLNACRPLLGLDRIYLKSKYLGTLLLATGFDGDGALFPLAFGVVDE 466
Query: 87 ETKEGWSFFLKNLRR----HVTKGISVCMVSDRHESIKSAFNDPRNGWQAT--GSAHVYC 140
E + W +FL L + + ++SDR + I +G +A + H +C
Sbjct: 467 ENDDNWMWFLSKLHNLLEINTENMPRLTILSDRQQGIV-------DGVEANFPTAFHGFC 625
Query: 141 IRHIKQNFMR 150
+RH+ NF +
Sbjct: 626 MRHLSDNFRK 655
>AL375901 similar to GP|9759134|dbj mutator-like transposase-like protein
{Arabidopsis thaliana}, partial (27%)
Length = 493
Score = 61.2 bits (147), Expect = 7e-10
Identities = 32/118 (27%), Positives = 56/118 (47%)
Frame = +3
Query: 135 SAHVYCIRHIKQNFMRTIKDGDLKDVVNNMGYALNVPLFNYYRGVIQEANQRALDWVDNI 194
++H +C+R++ +NF T K+ L ++ N YAL F + E +Q + W +
Sbjct: 18 ASHGFCLRYVSENFRDTFKNTKLVNIFWNAVYALTAAEFESKITEMIEVSQDVISWFQHF 197
Query: 195 PKEKWTQAYDEGRRWGHMTSNIVESWNSVFKGTRNLPVTPIVQSTYYRLACLFADRAQ 252
P W AY +G R+GH T + E + LPV +++ ++ F DR +
Sbjct: 198 PPFLWAVAYFDGVRYGHFTLGVTELLYNWALECHELPVVQMMEYIRQQMTSWFNDRRE 371
>AL375902 similar to GP|9759134|dbj mutator-like transposase-like protein
{Arabidopsis thaliana}, partial (20%)
Length = 542
Score = 50.4 bits (119), Expect = 1e-06
Identities = 36/132 (27%), Positives = 52/132 (39%), Gaps = 13/132 (9%)
Frame = +2
Query: 316 RAGWCDCGKFQALHLPCSHVIAACSSFRHDYTTLIPHVFKNESVYSIYNTAFKVVHDKSY 375
R+ C C ++Q PC+H AA S H+ F +S Y+ + DKS
Sbjct: 2 RSRECSCRRWQLYGYPCAHAAAALISCGHNAHMFAEPCFTVQSYRMAYSQMIYPIPDKSQ 181
Query: 376 WLPYDG----------PVICHNPNMRRLKKGRPNSTRIRTEMDEEVVERTPTPRR---CG 422
W + ++ H P +RR GRP +R VE P+R CG
Sbjct: 182 WREHGEGAEGGGGARVDIVIHPPKIRR-PPGRPKKKVLR-------VENFKRPKRVVQCG 337
Query: 423 LCRHTGHIRRNC 434
C GH ++ C
Sbjct: 338 RCHMLGHSQKKC 373
>BI309424 SP|P03749|VHS Host specificity protein J. {Bacteriophage lambda},
partial (8%)
Length = 651
Score = 34.7 bits (78), Expect = 0.075
Identities = 20/79 (25%), Positives = 40/79 (50%), Gaps = 10/79 (12%)
Frame = +1
Query: 63 TLLLAVAQDGNNKTIPIAFALVEGETKEGWSFFLKNLRRHVTKGI----------SVCMV 112
+LL +++Q + T+P +AL++G+T + ++ L G+ S +
Sbjct: 244 SLLCSLSQSWPSDTVPSGYALMQGQTFDKSAY--PKLAVAYPSGVMPDDGAPFRYSFSAL 417
Query: 113 SDRHESIKSAFNDPRNGWQ 131
DRH +++ + DP NGW+
Sbjct: 418 KDRHNAVEVNWIDPNNGWE 474
>CB065281 similar to SP|Q51422|SYD_ Aspartyl-tRNA synthetase (EC 6.1.1.12)
(Aspartate--tRNA ligase) (AspRS). {Pseudomonas
aeruginosa}, partial (32%)
Length = 599
Score = 31.2 bits (69), Expect = 0.83
Identities = 18/53 (33%), Positives = 25/53 (46%)
Frame = -1
Query: 381 GPVICHNPNMRRLKKGRPNSTRIRTEMDEEVVERTPTPRRCGLCRHTGHIRRN 433
GP + + R L+ RP S R+R + + PRRCG C+H RN
Sbjct: 437 GPGRVRSRSRRDLRCRRPRSQRVRRADHRQGAQ---APRRCGECQHGLRCHRN 288
>TC79947 similar to GP|15450948|gb|AAK96745.1 Unknown protein {Arabidopsis
thaliana}, partial (89%)
Length = 845
Score = 30.8 bits (68), Expect = 1.1
Identities = 23/82 (28%), Positives = 37/82 (45%), Gaps = 2/82 (2%)
Frame = +2
Query: 44 YCKPVVHVDGTWLYDKYKGTLLLAVAQDGNNKTIPIAFALVEGETKE--GWSFFLKNLRR 101
+C P + +LY LL VA+D N + + A +E ET E G L LR
Sbjct: 149 FCLPENYQMKYYLYHILSWPQLLYVAEDYNGRIVGYVLAKMEEETNECHGHITSLAVLRT 328
Query: 102 HVTKGISVCMVSDRHESIKSAF 123
H GI+ +++ +++ F
Sbjct: 329 HRKLGIATKLMTAAQNAMEQVF 394
>TC87026 weakly similar to GP|21592576|gb|AAM64525.1 SOUL-like protein
{Arabidopsis thaliana}, partial (64%)
Length = 794
Score = 30.4 bits (67), Expect = 1.4
Identities = 15/52 (28%), Positives = 28/52 (53%), Gaps = 2/52 (3%)
Frame = +1
Query: 176 YRGVIQEAN--QRALDWVDNIPKEKWTQAYDEGRRWGHMTSNIVESWNSVFK 225
+ G +++ N + A D+I KW+ A ++ RR GH + V +N+ F+
Sbjct: 580 FGGFVKDTNIGEEAAALKDSIAGTKWSSAIEQSRRAGHASVYSVAQYNAPFE 735
>TC81234 similar to GP|6752888|gb|AAF27920.1| unknown {Malus x domestica},
partial (50%)
Length = 1149
Score = 30.0 bits (66), Expect = 1.8
Identities = 13/30 (43%), Positives = 21/30 (69%)
Frame = -3
Query: 378 PYDGPVICHNPNMRRLKKGRPNSTRIRTEM 407
P+ PV+CH PNM + K+ RP + +I T++
Sbjct: 313 PFPKPVLCHAPNM*K-KQHRPTTIKICTKL 227
>TC86301 similar to GP|8809622|dbj|BAA97173.1 Myb-related transcription
activator-like {Arabidopsis thaliana}, partial (56%)
Length = 1396
Score = 29.3 bits (64), Expect = 3.2
Identities = 10/18 (55%), Positives = 11/18 (60%)
Frame = +2
Query: 419 RRCGLCRHTGHIRRNCPS 436
RRC C H GH R CP+
Sbjct: 191 RRCSHCSHNGHNSRTCPN 244
>TC81134 weakly similar to GP|14334664|gb|AAK59510.1 unknown protein
{Arabidopsis thaliana}, partial (42%)
Length = 900
Score = 29.3 bits (64), Expect = 3.2
Identities = 22/69 (31%), Positives = 34/69 (48%), Gaps = 6/69 (8%)
Frame = +2
Query: 323 GKFQALHLPCSHVIAACSSFRHDYTTLIPHVFK------NESVYSIYNTAFKVVHDKSYW 376
G F+A+ PC H I+ SSF YT+L F N+S+ SI + + + + +
Sbjct: 428 GVFRAISFPCIHPIS*SSSF--FYTSLTSDTFSVTTA*DNQSIISISEP*YSICNARKWP 601
Query: 377 LPYDGPVIC 385
L D +IC
Sbjct: 602 L*SDS-IIC 625
>TC88387 similar to GP|13357253|gb|AAK20050.1 putative zinc finger protein
{Oryza sativa (japonica cultivar-group)}, partial (96%)
Length = 1286
Score = 28.9 bits (63), Expect = 4.1
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 419 RRCGLCRHTGHIRRNCPS 436
+ C CR TGH+ R+CP+
Sbjct: 724 KACNNCRKTGHLARDCPN 777
Score = 28.5 bits (62), Expect = 5.4
Identities = 10/23 (43%), Positives = 12/23 (51%)
Frame = +1
Query: 416 PTPRRCGLCRHTGHIRRNCPSLN 438
P C LC +GH+ R CP N
Sbjct: 772 PNDPICNLCNISGHVARQCPKSN 840
>BF632724 similar to GP|20804478|db hypothetical protein~similar to
Arabidopsis thaliana chromosome 1 F6I1.10, partial
(27%)
Length = 539
Score = 28.5 bits (62), Expect = 5.4
Identities = 11/23 (47%), Positives = 13/23 (55%)
Frame = +2
Query: 23 EWRAQFHRLFWAFYPCINGFKYC 45
EW QF LFW+ Y + F YC
Sbjct: 386 EWI*QFQLLFWSCYAGLGKFAYC 454
>TC78787 similar to GP|13569996|gb|AAK31280.1 putative Myb-related protein
{Oryza sativa}, partial (47%)
Length = 1438
Score = 27.7 bits (60), Expect = 9.2
Identities = 10/18 (55%), Positives = 11/18 (60%)
Frame = +2
Query: 419 RRCGLCRHTGHIRRNCPS 436
RRC C + GH R CPS
Sbjct: 122 RRCSHCSNNGHNSRTCPS 175
>TC86179 similar to GP|6752888|gb|AAF27920.1| unknown {Malus x domestica},
partial (60%)
Length = 1297
Score = 27.7 bits (60), Expect = 9.2
Identities = 11/30 (36%), Positives = 21/30 (69%)
Frame = -3
Query: 378 PYDGPVICHNPNMRRLKKGRPNSTRIRTEM 407
P+ P++CH PN+ + K+ +P + +I TE+
Sbjct: 269 PFPKPLLCHAPNI*K-KQHKPTTIKICTEL 183
>TC86178 similar to GP|6752888|gb|AAF27920.1| unknown {Malus x domestica},
partial (29%)
Length = 691
Score = 27.7 bits (60), Expect = 9.2
Identities = 11/30 (36%), Positives = 21/30 (69%)
Frame = -1
Query: 378 PYDGPVICHNPNMRRLKKGRPNSTRIRTEM 407
P+ P++CH PN+ + K+ +P + +I TE+
Sbjct: 256 PFPKPLLCHAPNI*K-KQHKPTTIKICTEL 170
>TC87639 GP|9663153|emb|CAC01132.1 transport-secretion protein 2.2 (TTS-2.2)
{Homo sapiens}, partial (2%)
Length = 1522
Score = 27.7 bits (60), Expect = 9.2
Identities = 13/26 (50%), Positives = 13/26 (50%), Gaps = 4/26 (15%)
Frame = -1
Query: 413 ERTPTPRR----CGLCRHTGHIRRNC 434
E P PRR C C GHI RNC
Sbjct: 352 EGAPPPRRDMRECYSCHERGHIARNC 275
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.138 0.452
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,369,915
Number of Sequences: 36976
Number of extensions: 251998
Number of successful extensions: 1239
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1216
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1233
length of query: 440
length of database: 9,014,727
effective HSP length: 99
effective length of query: 341
effective length of database: 5,354,103
effective search space: 1825749123
effective search space used: 1825749123
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 60 (27.7 bits)
Medicago: description of AC126012.8